Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,619,403 G→A 8.0% intergenic (‑91/‑58) PP_2292 ← / → PP_2293 hypothetical protein/DNA maturase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,619,4030GA 8.0% 139.8 / 2.2 50intergenic (‑91/‑58)PP_2292/PP_2293hypothetical protein/DNA maturase B
Reads supporting (aligned to +/- strand):  ref base G (25/21);  new base A (2/2);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.59e-01

CAGTTTCAAAGATCCCTTCAAAAGCTTTCAAGTCGTTCAATTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCACCCCGCCCAGGAAGACTTTCGAAAGTTTATCTACCTGGTATGGAAACACCTCAACCTCCCCGAAC  >  NC_002947/2619258‑2619548
                                                                                                                                                 |                                                                                                                                                  
cAGTTTCAAAGATCCCTTCAAAAGCTTTCAAGTCGTTCAATTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGggcggc                                                                                                                                                  <  2:176215/148‑1 (MQ=255)
  gTTTCAAAGATCCCTTCAAAAGCTTTCAAGTCGTTCAATTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCtt                                                                                                                                               <  4:183948/149‑1 (MQ=255)
     tCAAAGATCCCTTCAAAAGCTTTCAAGTCGTTCAATTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCg                                                                                                                                            >  1:164885/1‑149 (MQ=255)
       aaaGATCCCTTCAAAAGCTTTCAAGTCGTTCAATTCGTTACGCTTCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGc                                                                                                                                          >  7:164506/1‑149 (MQ=255)
                  cAAAAGCTTTCAAGTCGTTCAATTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTgg                                                                                                                                <  4:32579/148‑1 (MQ=255)
                  cAAAAGCTTTCAAGTCGTTCAATTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTgg                                                                                                                                <  7:14690/148‑1 (MQ=255)
                               gTCGTTCAATTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCGTTGCGGCTGTGCCTTGGCTCGCTCC‑TGcgcg                                                                                                                 >  2:88451/1‑149 (MQ=255)
                               gTCGTTCAATTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCtgt                                                                                                                                       >  6:324849/1‑128 (MQ=255)
                               gTCGTTCAATTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCtgt                                                                                                                                       <  5:324849/128‑1 (MQ=255)
                                  gTTCAATTCGTTACGCTTCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCggg                                                                                                               <  8:164506/148‑1 (MQ=255)
                                  gTTCAATTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGc                                                                                                              >  5:337166/1‑149 (MQ=255)
                                  gTTCAATTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGc                                                                                                              >  3:165047/1‑149 (MQ=255)
                                   ttCAATTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGcc                                                                                                             >  3:350730/1‑149 (MQ=255)
                                              aCGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTccc                                                                                                  >  5:207406/1‑149 (MQ=255)
                                                gCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCtt                                                                                                >  3:46190/1‑149 (MQ=255)
                                                    cATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCgg                                                                                                                                           >  2:295311/1‑103 (MQ=255)
                                                    cATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGcc                                                                                            <  4:165047/149‑1 (MQ=255)
                                                    cATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGcc                                                                                            <  6:337166/149‑1 (MQ=255)
                                                      tttAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGCCg                                                                                           >  5:154216/1‑148 (MQ=255)
                                                          gTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGCCGTAtga                                                                                      >  8:323002/1‑149 (MQ=255)
                                                              aTGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGCCGTAtgactga                                                                                  >  3:355027/1‑149 (MQ=255)
                                                                  aTTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACcgc                                                                              >  2:107553/1‑149 (MQ=255)
                                                                  aTTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGCGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCGCTTTGCCGTATGACTGACcgc                                                                              >  6:15953/1‑149 (MQ=255)
                                                                            gtacCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCATGGGCGGCCTTGCGGATGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGAGTTAAAGtt                                                                     >  6:117404/1‑148 (MQ=255)
                                                                                ccTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCAccc                                                                >  5:160667/1‑149 (MQ=255)
                                                                                 cTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCAcccc                                                               >  5:226486/1‑149 (MQ=255)
                                                                                  tAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCACCCCg                                                              >  1:43554/1‑149 (MQ=255)
                                                                                        aGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGCCGTCtg                                                                                       <  5:280295/118‑1 (MQ=255)
                                                                                        aGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGCCGTAtg                                                                                       >  6:280295/1‑118 (MQ=255)
                                                                                         ggttctAATAAAATTTTTGCTCGTAAACAACAGGTTCCCCCAGGCGGGTCCTGGGCGGCCTTTCGGCTGTGCCTTGGCTCGCTCCTTGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCACCCCGCCCAg                                                         <  1:107553/144‑1 (MQ=255)
                                                                                                ataaAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCACCCCGCCCAGGAAGACttt                                                <  2:164885/149‑1 (MQ=255)
                                                                                                ataaAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCACCCCGCCCAGGAAGACtt                                                 <  4:350730/148‑1 (MQ=255)
                                                                                                 taaAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCACCCCGCCCAGGAAGACTTTc                                               <  4:46190/149‑1 (MQ=255)
                                                                                                  aaaaTTTTTGCTCGTAAACAACAGGCGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCACCCCGcc                                                            >  1:266054/1‑135 (MQ=255)
                                                                                                  aaaaTTTTTGCTCGTAAACAACAGGCGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCACCCCGcc                                                            <  2:266054/135‑1 (MQ=255)
                                                                                                      tttttGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCttt                                                                                               <  5:324963/96‑1 (MQ=255)
                                                                                                      tttttGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCttt                                                                                               >  6:324963/1‑96 (MQ=255)
                                                                                                            cTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCACCCCGCCCAGGAAGACTTTCGAAAGTTTATc                                    <  6:207406/149‑1 (MQ=255)
                                                                                                             tCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCAGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGAGTGACCGCGTTAAAGTTc                                                                    <  8:73089/116‑1 (MQ=255)
                                                                                                             tCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCAGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTc                                                                    <  2:268100/116‑1 (MQ=255)
                                                                                                             tCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCAGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTc                                                                    >  7:73089/1‑116 (MQ=255)
                                                                                                             tCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCAGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTc                                                                    >  1:268100/1‑116 (MQ=255)
                                                                                                                   acaacaGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCACCCCGCCCAGGAAGACTTTCGAAAGTTTATCTACCTgg                             >  2:33958/1‑149 (MQ=255)
                                                                                                                    caacaGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCACCCCGCCCAGGAAGACTTTCGAAAGTTTATCTACCTGGt                            <  6:226486/149‑1 (MQ=255)
                                                                                                                     aacaGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGTCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCACCCCGCCCAGGAAGACTTTCGAAAGTTTATCTACCTGGTa                           <  5:188685/149‑1 (MQ=255)
                                                                                                                     aacaGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCACCCCGCCCAGGAAGACTTTCGAAAGTTTATCTACCTGGTa                           >  5:55821/1‑149 (MQ=255)
                                                                                                                          gTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGCCGTAtgac                                                                                     >  3:102737/1‑86 (MQ=255)
                                                                                                                          gTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGCCGTAtgac                                                                                     <  4:102737/86‑1 (MQ=255)
                                                                                                                              ccccAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCACCCCGCCCAGGAAGACTTTCGAAAGTTTATCTACCTGGTATGGAAacac                  <  2:43554/149‑1 (MQ=255)
                                                                                                                                   ggCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGATCGCGTTAAAGTTCACCCCGCCCAGGAAGACTTTCGAAAGTTTATCTACCTGGTATGGAAACACCTCaa             <  7:323002/149‑1 (MQ=255)
                                                                                                                                        gTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCACCCCGCCCAGGAAGACTTTCGAAAGTTTATCTACCTGGTATGGAAACACCTCAACCTcc        <  6:55821/149‑1 (MQ=255)
                                                                                                                                              ggcggcCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCACCCCGCCCAGGAAGACTTTCGAAAGTTTATCTACCTGGTATGGAAACACCTCAACCTCCCCGaa   <  1:33958/148‑1 (MQ=255)
                                                                                                                                              ggcggcCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCACCCCGCCCAGGAAGACTTTCGAAAGTTTATCTACCTGGTATGGAAACACCTCAACCTCCCCGAac  <  6:154216/149‑1 (MQ=255)
                                                                                                                                                cggcCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCACCCCGCCCAGGAAGACTTTCGAAAGTTTATCTACCTGGTATGGAAacac                  <  7:133180/131‑1 (MQ=255)
                                                                                                                                                cggcCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCACCCCGCCCAGGAAGACTTTCGAAAGTTTATCTACCTGGTATGGAAacac                  >  8:133180/1‑131 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CAGTTTCAAAGATCCCTTCAAAAGCTTTCAAGTCGTTCAATTCGTTACGCTCCATTTAGTGAATGCATTTCCGTAGGTAGCCTAGTCTAGCTGCTAATAAAATTTTTGCTCGTAAACAACAGGTGCCCCCAGGCGCGTCCTGGGCGGCCTTGCGGCTGTGCCTTGGCTCGCTCC‑TGCGCGGGCCCAAGGAGTCCCTTTGCCGTATGACTGACCGCGTTAAAGTTCACCCCGCCCAGGAAGACTTTCGAAAGTTTATCTACCTGGTATGGAAACACCTCAACCTCCCCGAAC  >  NC_002947/2619258‑2619548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑