Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,682,917	G→C	9.3%	D495H (GAT→CAT)	prpE →	propionyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,682,917	0	G	C	9.3%	155.9 / 4.8	53	D495H (GAT→CAT)	prpE	propionyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base G (23/25);  new base C (2/3);  total (25/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

TTGCCACCCGGTTGCAGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGGTGTGCATGACGAGATCAAGGGCCAGGTACCGTTGGCGCTGGTGGTACTCAA  >  NC_002947/2682773‑2683065                                                                                                                                                  |                                                                                                                                                     ttGCCCCCCGGTTGCAGTCAGACGCTGTGGGGGGATCCCGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTGCGTCTACATCAT‑GGGGCGCACCgatga                                                                                                                                                  >  5:183211/1‑149 (MQ=255) ttGCCACCCGGTTGCAGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCgatga                                                                                                                                                  >  3:310994/1‑149 (MQ=255) ttGCCACCCGGTTGCAGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCgatga                                                                                                                                                  <  4:101980/149‑1 (MQ=255) ttGCCACCCGGTTGCAGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCgatga                                                                                                                                                  >  4:96041/1‑149 (MQ=255)     cACCCGGTTGCAGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACATGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCTCACCGATGAtgt                                                                                                                                               >  4:349616/1‑148 (MQ=255)          ggTTGCAGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCATCGGTGCGC‑CCCATGAtgtagacga                                                                                                                                         <  7:68675/149‑7 (MQ=255)          ggTTGCAGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCaa                                                                                                                                         <  4:28462/149‑1 (MQ=255)             tGCAGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGt                                                                                                                                      >  4:130062/1‑149 (MQ=255)                aGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCATCGGTGCGC‑CCCATGAtgt                                                                                                                                               >  2:32059/1‑137 (MQ=255)                aGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTCCGTCTACATCATCGGTGCGC‑CCCATGAtgt                                                                                                                                               <  1:32059/137‑1 (MQ=255)                 gTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGTCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTcc                                                                                                                                  <  2:316969/149‑1 (MQ=255)                          tgtgGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGt                                                                                                                         >  5:254721/1‑149 (MQ=255)                            tgGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGt                                                                                                                         <  4:310994/147‑1 (MQ=255)                              gggggATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGt                                                                                                                     >  8:15313/1‑149 (MQ=255)                                 ggATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCa                                                                                                                  >  1:163086/1‑149 (MQ=255)                                             cTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCATCGGTGCGC‑CCCATGAtgta                                                                                                                                              >  4:186357/1‑108 (MQ=255)                                             cTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCATCGGTGCGC‑CCCATGAtgta                                                                                                                                              <  3:186357/109‑2 (MQ=255)                                              tACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATgg                                                                                                      >  4:331732/1‑148 (MQ=255)                                                 cTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGa                                                                                                  >  5:39464/1‑149 (MQ=255)                                                   gCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGAcc                                                                                                >  1:54548/1‑149 (MQ=255)                                                   gCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGAcc                                                                                                >  3:47784/1‑149 (MQ=255)                                                     aGGCCTACCTGCTCACGTATCCTGTGTACTACCAGACGGGCTATGGCGGCTATCTGTCAGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCt                                                                                               <  3:349616/148‑1 (MQ=255)                                                     aGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTg                                                                                              <  3:96041/149‑1 (MQ=255)                                                          tACCTGCGCACGTATCCGGGGTACTACCCCACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTcgc                                                                                         >  4:331023/1‑149 (MQ=255)                                                                 gcACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCAc                                                                                  >  4:58605/1‑149 (MQ=255)                                                                   aCGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCAccc                                                                                >  8:6407/1‑149 (MQ=255)                                                                   aCGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCAccc                                                                                >  1:66111/1‑149 (MQ=255)                                                                          cGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATgg                                                                                                      <  1:318409/120‑1 (MQ=255)                                                                          cGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATgg                                                                                                      >  2:318409/1‑120 (MQ=255)                                                                            gggTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGc                                                                       <  7:15313/149‑1 (MQ=255)                                                                            gggTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGc                                                                       <  3:130062/149‑1 (MQ=255)                                                                             ggTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCg                                                                      <  5:189146/149‑1 (MQ=255)                                                                                 cttccACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAgt                                                                  <  6:254721/146‑1 (MQ=255)                                                                                   acCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAgtgt                                                                >  4:120684/1‑149 (MQ=255)                                                                                    ccACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGa                                                                    <  4:277153/144‑1 (MQ=255)                                                                                    ccACACGGGCGATGGCGGCTAGCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGa                                                                    >  3:277153/1‑144 (MQ=255)                                                                                      acacGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAgt                                                                  >  1:286008/1‑144 (MQ=255)                                                                                      acacGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAgt                                                                  <  2:286008/144‑1 (MQ=255)                                                                                       cacGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGGCACCCGGCGGTAGCGGAGTTTGCtg                                                            <  2:66111/149‑1 (MQ=255)                                                                                       cacGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCtg                                                            <  2:54548/149‑1 (MQ=255)                                                                                        acGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCcggcg                                                                            >  2:257341/1‑132 (MQ=255)                                                                                        acGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGATTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCcggcg                                                                            <  1:257341/132‑1 (MQ=255)                                                                                               aTGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGgt                                                    <  7:6407/149‑1 (MQ=255)                                                                                                 ggcggcTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGgtg                                                   <  6:39464/148‑1 (MQ=255)                                                                                                     gcTATCTTGACTATGATGTTTTCGTCTACATCAT‑GGTGCGGACCGATTATGTGATCTACGTTTCCCGTCATCGTTTGTCCACCGGTTAAATGGAAGACCTGGTCGCCCGCCAACCGGCGGTAGCGGAGTGTGCTGTGATCGGTGTGCAt                                              <  7:95529/149‑1 (MQ=255)                                                                                                                gatgatGGTTTCGTCTACATCAT‑GGGGCGGACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGGTGTGCATGACGAGATCaa                                   <  3:120684/149‑1 (MQ=255)                                                                                                                gatgatGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGGTGTGCATGACGAGATCaa                                   <  3:331023/149‑1 (MQ=255)                                                                                                                   gatgGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCGACCGGTGATATGGAAGAGCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGGTGTGCATGACGAGATCAAggg                                >  2:223101/1‑149 (MQ=255)                                                                                                                    atgGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGGTGTGCATGACGAGATCAAGGGc                               >  4:489/1‑149 (MQ=255)                                                                                                                       gTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGGTGTGCATGACGAGATCAAGGGCCAg                            <  3:58605/149‑1 (MQ=255)                                                                                                                                  atcat‑GGGGCGCACCGATGATGTGATCAACGTTCCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGgtg                                                   <  2:82873/115‑1 (MQ=255)                                                                                                                                  atcat‑GGGGCGCACCGATGATGTGATCAACGTTACCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGgtg                                                   >  1:82873/1‑115 (MQ=255)                                                                                                                                        ggggCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCCGTAGCGGAGTGTGCTGTGATCGGTGTGCATGACGAGATCAAGGGCCACGTACCGTTGGCGCtgg            >  6:297948/1‑149 (MQ=255)                                                                                                                                         gggCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGGTGTGCATGACGAGATCAAGGGCCAGGTACCGTTGGCGCtggt           <  3:489/149‑1 (MQ=255)                                                                                                                                          ggCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGGTGTGCATGACGAGATCAAGGGCCAGGTACCGTTGGCGCtggcg          <  3:331732/149‑3 (MQ=255)                                                                                                                                               aCCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGGTGTGCATGACgaga                                       <  6:89659/115‑1 (MQ=255)                                                                                                                                               aCCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGGTGTGCATGACgaga                                       >  5:89659/1‑115 (MQ=255)                                                                                                                                                  gattatGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGGTGTGCATGACGAGATCAAGGGCCAGGTACCGTTGGCGCTTGTGGTACTCaa  <  4:47784/149‑1 (MQ=255)                                                                                                                                                  gatgatGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGGTGTGCATGACGAGATCAAGGGCCAGGTACCGTTGGCGCTGGTGGTACTc    >  3:276896/1‑147 (MQ=255)                                                                                                                                                  |                                                                                                                                                     TTGCCACCCGGTTGCAGTCAGACGCTGTGGGGGGATCACGAGCGCTACCTGCAGGCCTACCTGCGCACGTATCCGGGGTACTACCACACGGGCGATGGCGGCTATCTGGACGATGATGGTTTCGTCTACATCAT‑GGGGCGCACCGATGATGTGATCAACGTTTCCGGTCATCGTTTGTCCACCGGTGAAATGGAAGACCTGGTCGCCCGCCACCCGGCGGTAGCGGAGTGTGCTGTGATCGGTGTGCATGACGAGATCAAGGGCCAGGTACCGTTGGCGCTGGTGGTACTCAA  >  NC_002947/2682773‑2683065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑