Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,710,330 G→T 8.5% V303V (GTG→GTT)  PP_2372 → sugar‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,710,3300GT 8.5% 144.8 / 3.0 47V303V (GTG→GTT) PP_2372sugar‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (25/18);  new base T (2/2);  total (27/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

CTGCCGTCAACAGCTCGCCCGAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCG‑TACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTG  >  NC_002947/2710182‑2710477
                                                                                                                                                     |                                                                                                                                                   
cTGCCGTCAACAGCTCGCCCGAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCG‑TACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTg                                                                                                                                                     <  6:47705/149‑1 (MQ=255)
             cTCGCCCGAAGCCTTGCGCGCGCGGATCGATGGGCGCCTGAGCG‑TACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTTCGCGCACGCCGtc                                                                                                                                        <  1:90339/149‑2 (MQ=255)
              tCGCCCGAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCG‑TACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTAt                                                                                                                                       >  5:348068/1‑149 (MQ=255)
                  ccGAAGCCTTGCGGGGGCGAATCGTTGGGCGCCTTTGCGTTCCAGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTTCGCGCACGCCGtcgcgg                                                                                                                                    <  1:120256/149‑6 (MQ=255)
                  ccGAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCG‑TACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCg                                                                                                                                   >  8:194455/1‑149 (MQ=255)
                  ccGAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCG‑TACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCg                                                                                                                                   >  1:862/1‑149 (MQ=255)
                            gcgcgcgcGTATCGATGGGCGCCTGAGCG‑TACTGATGTGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCa                                                                                                                           <  6:348068/147‑1 (MQ=255)
                            gcgcgcgcGTATCGATGGGCGCCTGAGCG‑TACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCa                                                                                                                           <  6:266175/147‑1 (MQ=255)
                            gcgcgcgcGTATCGATGGGCGCCTGAGCG‑TACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCa                                                                                                                           <  2:862/147‑1 (MQ=255)
                            gcgcgcgcGTATCGATGGGCGCCTGAGCG‑TACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAg                                                                                                                          <  5:354350/148‑1 (MQ=255)
                                gcgcgTATCGATGGGCGCCTGAGCG‑TACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAAt                                                                                                                     <  4:129895/149‑1 (MQ=255)
                                    gTATCGATGGGCGCCTGAGTG‑TACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGCAGTTGAACCGCGACGCCGTGCGCGAACGCCGTATTCCGGTa                                                                                                                                >  1:244142/1‑134 (MQ=255)
                                     tATCGATGGGCGCCTGAGCG‑TACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGAt                                                                                                                 >  8:242480/1‑148 (MQ=255)
                                              gcgcCTGAGCG‑TACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCAGGTATTGCAGGAAATCGATCaggccaagg                                                                                                       >  6:329078/1‑149 (MQ=255)
                                                               aTGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCAGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGaa                                                                                       <  5:329078/149‑1 (MQ=255)
                                                                    tgggCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGACAAGGCCAGGAGTTGGCTGAAGCTGt                                                                                  >  8:233622/1‑149 (MQ=255)
                                                                                ccTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGcc                                                                                                                                           >  8:221367/1‑80 (MQ=255)
                                                                                ccTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGcc                                                                                                                                           <  7:221367/80‑1 (MQ=255)
                                                                                ccTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGgac                                                                       >  3:236548/1‑148 (MQ=255)
                                                                                  ttGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTCGCGTTGAACCGCGACGGCGTGCCCGAACGCCGTATTCCGGTATTCCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGgacgac                                                                    >  3:20077/1‑149 (MQ=255)
                                                                                           gggCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGt                                                            <  4:236548/148‑1 (MQ=255)
                                                                                                  ggTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTc                                                    >  2:284640/1‑149 (MQ=255)
                                                                                                     gATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGgacga                                                                     <  8:211304/129‑1 (MQ=255)
                                                                                                     gATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGgacga                                                                     >  7:211304/1‑129 (MQ=255)
                                                                                                      aTGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCg                                                             <  4:265242/136‑1 (MQ=255)
                                                                                                      aTGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCg                                                             >  3:265242/1‑136 (MQ=255)
                                                                                                       tgctgcACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGt                                               <  7:233622/149‑1 (MQ=255)
                                                                                                          tgcACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTa                                             >  6:223235/1‑148 (MQ=255)
                                                                                                               gatgatGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGAtttt                                                     >  1:295588/1‑135 (MQ=255)
                                                                                                               gatgatGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGAtttt                                                     <  2:295588/135‑1 (MQ=255)
                                                                                                                  gatgCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCg                                     >  3:357928/1‑148 (MQ=255)
                                                                                                                       ccACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGggc                               >  1:273074/1‑149 (MQ=255)
                                                                                                                       ccACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGggc                               >  3:80972/1‑149 (MQ=255)
                                                                                                                         aCGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGggcgg                             <  4:357928/149‑1 (MQ=255)
                                                                                                                             gCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGggcgg                             >  2:57700/1‑145 (MQ=255)
                                                                                                                             gCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGggcgg                             <  1:57700/145‑1 (MQ=255)
                                                                                                                                 gCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCgg                     <  7:242480/149‑1 (MQ=255)
                                                                                                                                 gCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCgg                     >  8:155480/1‑149 (MQ=255)
                                                                                                                                 gCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCgg                     >  5:256955/1‑149 (MQ=255)
                                                                                                                                   gTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTa                                             >  7:161627/1‑123 (MQ=255)
                                                                                                                                   gTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTa                                             <  8:161627/123‑1 (MQ=255)
                                                                                                                                    ttGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTa                  >  1:278086/1‑149 (MQ=255)
                                                                                                                                          cgcgACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTa            <  1:284640/149‑1 (MQ=255)
                                                                                                                                             taCGGCGTTCGCGCACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTAccc         >  1:344873/2‑149 (MQ=255)
                                                                                                                                             gACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTAccc         >  6:86843/1‑149 (MQ=255)
                                                                                                                                               cGGCGTTCGCGCACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGATGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGACGAAGGGCGGCCGCCCGGGTACCGTTACCCGt       >  2:309817/1‑149 (MQ=255)
                                                                                                                                                   gTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGattat   >  7:226737/1‑147 (MQ=255)
                                                                                                                                                    tGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTg  <  2:273074/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CTGCCGTCAACAGCTCGCCCGAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCG‑TACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTG  >  NC_002947/2710182‑2710477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑