Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,710,335 A→C 8.2% E305A (GAA→GCA)  PP_2372 → sugar‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,710,3350AC 8.2% 148.2 / 4.7 49E305A (GAA→GCA) PP_2372sugar‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (26/19);  new base C (2/2);  total (28/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01

CTCGCCCGAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCG‑TACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTC  >  NC_002947/2710195‑2710482
                                                                                                                                             |                                                                                                                                                   
cTCGCCCGAAGCCTTGCGCGCGCGGATCGATGGGCGCCTGAGCG‑TACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTTCGCGCACGCCGtc                                                                                                                                             <  1:90339/149‑2 (MQ=255)
 tCGCCCGAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCG‑TACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTAt                                                                                                                                            >  5:348068/1‑149 (MQ=255)
     ccGAAGCCTTGCGGGGGCGAATCGTTGGGCGCCTTTGCGTTCCAGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTTCGCGCACGCCGtcgcgg                                                                                                                                         <  1:120256/149‑6 (MQ=255)
     ccGAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCG‑TACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCg                                                                                                                                        >  8:194455/1‑149 (MQ=255)
     ccGAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCG‑TACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCg                                                                                                                                        >  1:862/1‑149 (MQ=255)
               gcgcgcgcGTATCGATGGGCGCCTGAGCG‑TACTGATGTGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCa                                                                                                                                <  6:348068/147‑1 (MQ=255)
               gcgcgcgcGTATCGATGGGCGCCTGAGCG‑TACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCa                                                                                                                                <  6:266175/147‑1 (MQ=255)
               gcgcgcgcGTATCGATGGGCGCCTGAGCG‑TACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCa                                                                                                                                <  2:862/147‑1 (MQ=255)
               gcgcgcgcGTATCGATGGGCGCCTGAGCG‑TACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAg                                                                                                                               <  5:354350/148‑1 (MQ=255)
                   gcgcgTATCGATGGGCGCCTGAGCG‑TACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAAt                                                                                                                          <  4:129895/149‑1 (MQ=255)
                       gTATCGATGGGCGCCTGAGTG‑TACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGCAGTTGAACCGCGACGCCGTGCGCGAACGCCGTATTCCGGTa                                                                                                                                     >  1:244142/1‑134 (MQ=255)
                        tATCGATGGGCGCCTGAGCG‑TACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGAt                                                                                                                      >  8:242480/1‑148 (MQ=255)
                                 gcgcCTGAGCG‑TACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCAGGTATTGCAGGAAATCGATCaggccaagg                                                                                                            >  6:329078/1‑149 (MQ=255)
                                                  aTGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCAGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGaa                                                                                            <  5:329078/149‑1 (MQ=255)
                                                       tgggCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGACAAGGCCAGGAGTTGGCTGAAGCTGt                                                                                       >  8:233622/1‑149 (MQ=255)
                                                                   ccTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGcc                                                                                                                                                <  7:221367/80‑1 (MQ=255)
                                                                   ccTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGcc                                                                                                                                                >  8:221367/1‑80 (MQ=255)
                                                                   ccTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGgac                                                                            >  3:236548/1‑148 (MQ=255)
                                                                     ttGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTCGCGTTGAACCGCGACGGCGTGCCCGAACGCCGTATTCCGGTATTCCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGgacgac                                                                         >  3:20077/1‑149 (MQ=255)
                                                                              gggCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGt                                                                 <  4:236548/148‑1 (MQ=255)
                                                                                     ggTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTc                                                         >  2:284640/1‑149 (MQ=255)
                                                                                        gATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGgacga                                                                          >  7:211304/1‑129 (MQ=255)
                                                                                        gATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGgacga                                                                          <  8:211304/129‑1 (MQ=255)
                                                                                         aTGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCg                                                                  <  4:265242/136‑1 (MQ=255)
                                                                                         aTGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCg                                                                  >  3:265242/1‑136 (MQ=255)
                                                                                          tgctgcACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGt                                                    <  7:233622/149‑1 (MQ=255)
                                                                                             tgcACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTa                                                  >  6:223235/1‑148 (MQ=255)
                                                                                                  gatgatGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGAtttt                                                          >  1:295588/1‑135 (MQ=255)
                                                                                                  gatgatGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGAtttt                                                          <  2:295588/135‑1 (MQ=255)
                                                                                                     gatgCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCg                                          >  3:357928/1‑148 (MQ=255)
                                                                                                          ccACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGggc                                    >  1:273074/1‑149 (MQ=255)
                                                                                                          ccACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGggc                                    >  3:80972/1‑149 (MQ=255)
                                                                                                            aCGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGggcgg                                  <  4:357928/149‑1 (MQ=255)
                                                                                                                gCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGggcgg                                  <  1:57700/145‑1 (MQ=255)
                                                                                                                gCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGggcgg                                  >  2:57700/1‑145 (MQ=255)
                                                                                                                    gCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCgg                          <  7:242480/149‑1 (MQ=255)
                                                                                                                    gCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCgg                          >  8:155480/1‑149 (MQ=255)
                                                                                                                    gCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCgg                          >  5:256955/1‑149 (MQ=255)
                                                                                                                      gTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTa                                                  >  7:161627/1‑123 (MQ=255)
                                                                                                                      gTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTa                                                  <  8:161627/123‑1 (MQ=255)
                                                                                                                       ttGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTa                       >  1:278086/1‑149 (MQ=255)
                                                                                                                             cgcgACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTa                 <  1:284640/149‑1 (MQ=255)
                                                                                                                                taCGGCGTTCGCGCACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTAccc              >  1:344873/2‑149 (MQ=255)
                                                                                                                                gACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTAccc              >  6:86843/1‑149 (MQ=255)
                                                                                                                                  cGGCGTTCGCGCACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGATGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGACGAAGGGCGGCCGCCCGGGTACCGTTACCCGt            >  2:309817/1‑149 (MQ=255)
                                                                                                                                      gTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGattat        >  7:226737/1‑147 (MQ=255)
                                                                                                                                       tGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTg       <  2:273074/149‑1 (MQ=255)
                                                                                                                                         cgcgAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGc     <  5:223235/149‑1 (MQ=255)
                                                                                                                                            gAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGACGGGCGGCCGCCCGGGTCCCGTTTCCCGTTTCTGGCggc  >  4:47281/1‑147 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
CTCGCCCGAAGCCTTGCGCGCGCGTATCGATGGGCGCCTGAGCG‑TACTGATGGGTGGGCATTTCACCCTTGGCGGTTGGGCCATGGTGATGCTGCACGATGATGCCCACGGGCTGGCGTTGAACCGCGACGGCGTGCGCGAACGCCGTATTCCGGTATTGCAGGAAATCGATCAGGCCAAGGCCAGGCGTTGGCTGAAGCTGTTGGAAAGGGACGACTACGGCGTCGATTTTCGGCGTTACAGTGCCGAAGGGCGGCCGCCCGGGTACCGTTACCCGTTTCTGGCGTC  >  NC_002947/2710195‑2710482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑