| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 204,976 | G→C | 11.1% | V3494V (GTG→GTC) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 204,976 | 0 | G | C | 11.1% | 146.4 / 10.5 | 54 | V3494V (GTG→GTC) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base G (23/24); new base C (2/4); total (25/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.81e-01 | |||||||||||
CGCTGGCCAGATCAGCCTGGGTATCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGCCGATCAATAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAA > NC_002947/204829‑205077 | cgCTGGCCAGATCAGCCTGGGTATCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGa < 1:339030/149‑1 (MQ=255) gATCCGCCTGGGTATCACGTCTCCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTCACCGAAgggg < 4:199362/149‑3 (MQ=255) gATCAGCCTGGGTATCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTCACCGAAgggg < 7:38315/149‑3 (MQ=255) aGCCTGGGTATCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGATCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTga < 6:266653/149‑1 (MQ=255) gCCTGGGTATCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTgag < 3:251837/149‑1 (MQ=255) gTATCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCACt < 8:107989/149‑1 (MQ=255) tATCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCg > 4:5686/1‑137 (MQ=255) tATCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCg < 3:5686/137‑1 (MQ=255) aTCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCACTTa > 7:314019/1‑149 (MQ=255) tCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCACTTa > 7:84312/1‑148 (MQ=255) cACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCACTTaca > 4:281156/1‑149 (MQ=255) aCGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCACTTaca < 8:84312/148‑1 (MQ=255) ctgTGGATGTAGACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTAcc > 8:107041/1‑149 (MQ=255) tgGATGTAGACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTCACCGAAgg > 3:95611/1‑121 (MQ=255) tgGATGTAGACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTCACCGAAgg < 4:95611/121‑1 (MQ=255) gATGTAGACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGAc < 8:314019/149‑1 (MQ=255) gATGTAGACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACAGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGCAGGCGGTGAGATCACTTACACCATTACACTGAc > 2:103441/1‑149 (MQ=255) gTAGACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACca > 3:244706/1‑148 (MQ=255) aGACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGATCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCACTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACcaac > 2:339442/1‑148 (MQ=255) gACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTCACCGAAggg < 3:107318/114‑2 (MQ=255) gACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTCACCGAAggg > 4:107318/1‑113 (MQ=255) tCGCACTTTAGAAAACCTGGAGCTTGGCGGTGGGGCTTCGTTTAAATTTACGTATACAACCGACTAAGTTGTGGCCATGTTTACCGCTATACCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGAc < 1:103441/149‑1 (MQ=17) gCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCGAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGt > 8:118644/1‑110 (MQ=255) gCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCGAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGt < 7:118644/110‑1 (MQ=255) gCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTTGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCAGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGc < 7:7759/149‑1 (MQ=255) gCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACaaa > 6:83635/1‑143 (MQ=255) gCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACaaa < 5:83635/143‑1 (MQ=255) aCCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCtt > 5:193443/1‑149 (MQ=255) ccTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTg > 2:312659/1‑149 (MQ=255) ttCGAAAACCTGGAGCTGGGCGGTGCGGATTCGGTACAAGTGACCGATACAACCGACGAAGTGGTGGCCAAGTTGACCCCGA‑CCCATACGGTGACCGAAGGCGGTGAGATCAGTTCCACCATTACCCTGACAAACAAAAACGGCTTGcc > 6:133182/1‑149 (MQ=17) cGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGcc < 7:107041/147‑1 (MQ=255) cGAAAACCTGCACCTGGGCGGGGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGCCGa < 8:121879/149‑1 (MQ=255) gAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGCCGAt < 4:244706/149‑1 (MQ=255) aCCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGCCGATCaaa > 5:73691/1‑148 (MQ=255) aCCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGCCGATCa < 6:73691/147‑1 (MQ=255) gAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCa < 7:152363/100‑1 (MQ=40) gAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCa > 8:152363/1‑100 (MQ=40) gCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGCCGATCAATAACcaca < 4:31324/149‑1 (MQ=255) ggCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGCCGATCAATAACCACAGTGc < 3:281156/149‑1 (MQ=255) ggTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGCCGATCAATAACCACAGTGCAtt < 1:312659/149‑1 (MQ=255) cGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGCCGATCAATAAc > 6:212415/1‑134 (MQ=255) cGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGCCGATCAATAAc < 5:212415/134‑1 (MQ=255) cTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGc > 7:189745/1‑120 (MQ=255) cTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGc < 8:189745/120‑1 (MQ=255) ttCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGa < 2:357563/111‑1 (MQ=255) ttCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGa > 1:357563/1‑111 (MQ=255) ttCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGCCGATCAATAACCACAGTGCATTGAc > 6:333521/1‑144 (MQ=255) ttCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGCCGATCAATAACCACAGTGCATTGAc < 5:333521/144‑1 (MQ=255) gTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGCCGATCAATAACCACAGTGCATTGACCTTCACGCt < 5:133182/149‑1 (MQ=39) aGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTTACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACcaa < 3:7245/96‑1 (MQ=255) aGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGCCGATCAATAACCACAGTGCATTGACCTTCACGCTGAGc > 5:291734/1‑148 (MQ=35) gTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGCCGATCAATAACCACAGTGCATTGACCTTCACGCTGAGCGa > 8:274110/1‑149 (MQ=34) aCCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGCCGATCAATAACCACAGTGCATTGACCTTCACGCTGAGCGACgg > 6:80660/1‑149 (MQ=32) aTACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGCCGATCAATAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCaa > 1:9225/1‑148 (MQ=31) | CGCTGGCCAGATCAGCCTGGGTATCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGA‑CCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGCCGATCAATAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAA > NC_002947/204829‑205077 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |