Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,724,716 A→T 7.9% intergenic (‑125/+23) azlC ← / ← PP_2386 branched‑chain amino acid transport protein AzlC/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,724,7160AT 7.9% 189.9 / 4.8 63intergenic (‑125/+23)azlC/PP_2386branched‑chain amino acid transport protein AzlC/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (29/29);  new base T (2/3);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

CTTGGCGGGCGATGTGGGGCATGATGGCTGCATGACGGCTACGAGGCAGGCCAGCATGACAGACGGGGCAGGGGATGGCTTGAACGATCTTGCTGGTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTGCGCAGCCATTGATGTCTACGGCTTTCTGCAGCAGGCTCGTGCGCTGCTGTGGGTCGAGGTCGCCAAGCAGGCGGTACACCTCGGCCTG  >  NC_002947/2724570‑2724861
                                                                                                                                                  |                                                                                                                                                 
cTTGGCGGGCGATGTGGGGCATGATGGCTGCATGACGGCTACGAGGCAGGCCAGCATGACAGACGGGGCAGGGGATGGCTTGAACGATCTTGCTGGTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAgg                                                                                                                                                 <  3:156535/149‑1 (MQ=255)
   ggcgggcgATGTGGGGCATGATGGCTGCATGACGGCTACGAGGCAGGCCAGCATGACAGACGGGGCAGGGGATGGCTTGAACGATCTTGCTGGTCGAGGCGTGTGCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAggc                                                                                                                                                <  5:109/147‑1 (MQ=255)
   gccgggcgATGTGGGGCATGATGGCTGCATGACGGCTACGAGGCAGGCCAGCATGACAGACTGGGCAGGGGATGGATTGAACGATCTTGCTGGTCGAGTCGTGTTCGTCTCAACCTCAGTGCCCAACTTACAACAGGGCCGCAAgcagg                                                                                                                                              >  8:243652/3‑145 (MQ=255)
    gcgggcgATGTGGGGCATGATGGCTGCATGACGGCTACGAGGCAGGCCAGCATGACAGACGGGGCAGGGGATGGCTTGAACGATCTTGCTGGTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAggcggc                                                                                                                                             >  6:157990/1‑149 (MQ=255)
     cgggcgATGTGGGGCATGATGGCTGCATGACGGCTACGAGGCAGGCCAGCATGACAGACGGGGCAGGGGATGGCTTGAACGATCTTGCTGGTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCCTTGCGGcc                                                                                                                                            <  1:106415/149‑1 (MQ=255)
      gggcgATGTGGGGCATGATGGCTGCATGACGGCTACGAGGCAGGCCAGCATGACAGACGGGGCAGGGGATGGCTTGAACGATCTTGCTGGTTGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGccc                                                                                                                                           >  6:140284/1‑149 (MQ=255)
            tgtgGGGCATGATGGCTGCATGACGTCTACGTGGCAGTCCAGCATGTCAGACGTGTCAGGGGATGTCTTGAACGATCTTGCTGGTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCCTTGCGGccctgttgt                                                                                                                                     <  1:170907/149‑7 (MQ=255)
             gtgGGGCAGGATGGCTGCATGACGGCTACGAGGCAGGCCAGCATGACAGACGGGGCAGGGGATGGCTTGAACGATCTTGCTGGTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATg                                                                                                                                     <  8:288832/148‑1 (MQ=255)
                   cATGATGGCTGCATGACGGCTACGAGGCAGGCCAGCATGACAGACGGGGCAGGGGATGGCTTGAACGATCTTGCTGGTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTgg                                                                                                                              >  4:69767/1‑149 (MQ=255)
                         ggCTGCATGACGGCTACGAGGCAGGCCAGCATGACAGACGGGGCAGGGGATGGCTTGAACGATCTTGCTGGTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCCTTGCGGccct                                                                                                                                          >  5:103336/1‑130 (MQ=255)
                         ggCTGCATGACGGCTACGAGGCAGGCCAGCATGACAGACGGGGCAGGGGATGGCTTGAACGATCTTGCTGGTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCCTTGCGGccct                                                                                                                                          <  6:103336/131‑2 (MQ=255)
                          gCTGCATGACGGCTACGAGGCAGGCCAGCATGACAGACGGGGCAGGGGATGGCTTGAACGATCTTGCTGGTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATg                                                                                                                        <  5:157990/148‑1 (MQ=255)
                              cATGACGGCTACGAGGCAGGCCAGCATGACAGACGGGGCAGGGGATGGCTTGAACGATCTTGCTGGTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGggcgg                                                                                                                   <  1:232905/149‑1 (MQ=255)
                               aTGACGGCTACGAGGCAGGCCAGCATGACAGACGGGGCAGGGGATGGCTTGAACGATCTTGCTGGTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGggcggc                                                                                                                  >  8:282338/1‑149 (MQ=255)
                               aTGACGGCTACGAGGCAGGCCAGCATGACAGACGGGGCAGGGGATGGCTTGAACGATCTTGCTGGTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGggc                                                                                                                     <  3:197750/146‑1 (MQ=255)
                               aTGACGGCTACGAGGCAGGCCAGCATGACAGACGGGGCAGGGGATGGCTTGAACGATCTTGCTGGTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGggc                                                                                                                     >  4:197750/1‑146 (MQ=255)
                                  aCGGCTACGAGGCAGGCCAGCATGACAGACGGGGCAGGGGATGGCTTGAACGATCTTGCTGGTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTg                                                                                                                <  2:55519/148‑1 (MQ=255)
                                       tACGAGGCAGGCCAGCATGACAGACGGGGCAGGGGATGGCTTGAACGATCTTGCTGGTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTcag                                                                                                           >  8:21079/1‑148 (MQ=255)
                                           aggcaggcCAGCATGACAGACGGGGCAGGGGATGGCTTGAACGATCTTGCTGGTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTcagc                                                                                                          <  5:320335/145‑1 (MQ=255)
                                           aggcaggcCAGCATGACAGACGGGGCAGGGGATGGCTTGAACGATCTTGCTGGTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTcagc                                                                                                          >  6:320335/1‑145 (MQ=255)
                                               aggcCAGCATGACAGACGGGGCAGGGGATGGCTTGAACGATCTTGCTGGTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTgg                                                                                                                              >  8:267904/1‑121 (MQ=255)
                                               aggcCAGCATGACAGACGGGGCAGGGGATGGCTTGAACGATCTTGCTGGTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTgg                                                                                                                              <  7:267904/121‑1 (MQ=255)
                                               aggcCAGCATGACAGACGGGGCAGGGGATGGCTTGAACGATCTTGCTGGTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTg                                                                                                  >  2:311076/1‑149 (MQ=255)
                                                  ccAGCATGACAGACGGGGCAGGGGATGGCTTGAACGATCTTGCTGGTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTgcgc                                                                                               >  3:68101/1‑149 (MQ=255)
                                                      cATGACAGACGGGGCAGGGGATGGCTTGAACGATCTTGCTGGTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGtt                                                                                           <  4:13854/149‑1 (MQ=255)
                                                       aTGACAGACGGGGCAGGGGATGGCTTGAACGATCTTGCTGGTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTg                                                                                          >  4:348244/1‑149 (MQ=255)
                                                        tGACAGACGGGGCAGGGGATGGCTTGAACGATCTTGCTGGTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTgc                                                                                         >  1:132870/1‑149 (MQ=255)
                                                          aCAGACGGGGCAGGGGATGGCTTGAACGATCTTGCTGGTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTgc                                                                                         >  7:154221/1‑147 (MQ=255)
                                                          aCAGACGGGGCAGGGGATGGCTTGAACGATCTTGCTGGTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTgc                                                                                         <  8:154221/147‑1 (MQ=255)
                                                           cAGACGGGGCAGGGGATGGCTTGAACGATCTTGCTGGTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTGCGCa                                                                                      <  2:255361/149‑1 (MQ=255)
                                                                ggggCAGGGGATGGCTTGAACGATCTTGCTGGTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTGCGCAGCCAt                                                                                 <  2:132870/149‑1 (MQ=255)
                                                                  ggCAGGGGATGGCTTGAACGTTCTTGCTGGTCGTGGCGTGTTCGTCTCAACCTCAGTGGCCTACTGACATCAGGGCCGCAAGGCGGCCCCGAATGACCCTTGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTGCGCAGCCAt                                                                                 <  3:69767/147‑1 (MQ=255)
                                                                    cAGGGGATGGCTTGAACGATCTTGCTGGTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTGCGCAGCCATTGAt                                                                             <  1:311076/149‑1 (MQ=255)
                                                                      ggggATGGCTTGAACGATCTTGCTGGTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGTTGGTCAGCAGCTGTTGCGCCGTTGCGCAGCCATTGATGt                                                                           <  7:274740/149‑1 (MQ=255)
                                                                      ggggATGGCTTGAACGATCTTGCTGGTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTGCGCAGCCATTGATGt                                                                           <  7:243652/149‑1 (MQ=255)
                                                                             gCTTGAACGATCTTGCTGGTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTGCGCAGCCATTGATGTCTACgg                                                                     <  7:166007/148‑1 (MQ=255)
                                                                              cTTGAACGATCTTGCTGGTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTGCGCAGCCATTGATGTCTACGGCt                                                                   >  4:282734/1‑149 (MQ=255)
                                                                                           gCTGGTTGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTGCGCAGCCATTGATGTCTACGGCTTTCTgca                                                            <  5:140284/143‑1 (MQ=255)
                                                                                           gCTGGTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTGCGCAGCCATTGATGTCTACGGCTTTCTGCAGCAGGc                                                      >  7:299498/1‑149 (MQ=255)
                                                                                               gTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTGCGCAGCCATTGATGTCTACGGCTTTCTGCAGCAGGCTc                                                    <  3:348244/147‑1 (MQ=255)
                                                                                                tCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTGCGCAGCCATTGATGTCTACGGCTTTCTGCAGCAGGCTCGTg                                                 >  7:59358/1‑149 (MQ=255)
                                                                                                     gCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTGCGCAGCCATTGATGTCTACGGCTTTCTGCAGCAGGCTCGTGCgctg                                            <  7:21079/149‑1 (MQ=255)
                                                                                                         gtTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTGCGCAGCCATTGATGTCTACGGCTTTCTGCAGCAGGCTCGTGCGCTGCtgt                                        >  6:353443/1‑149 (MQ=255)
                                                                                                          ttCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTGCGCAGCCATTGATGTCTACGGCTTTCTGCAGCAGGCTCGTGCGCTGCtgt                                        <  5:353443/148‑1 (MQ=255)
                                                                                                            cGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTGCGCAGCCATTGATGTCTACGGCTTTCTGCAGCAgg                                                       >  6:17601/1‑131 (MQ=255)
                                                                                                            cGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTGCGCAGCCATTGATGTCTACGGCTTTCTGCAGCAgg                                                       <  5:17601/131‑1 (MQ=255)
                                                                                                             gTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTGCGCAGCCATTGATGTCTACGGCTTTCTGCAGCAGGCTCGTGCGCTGCTGTGGGt                                    >  2:85135/1‑149 (MQ=255)
                                                                                                                 cAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTGCGCAGCCATTGATGTCTACGGCTTTCTGCAGCAGGCTCGTGCGCTGCTGTGGGTCGAg                                >  5:67481/1‑149 (MQ=255)
                                                                                                                  aaCCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTGCGCAGCCATTGATGTCTACGGCTTTCTGCAGCAGGCTCGTGCGCTGCTGTGGGTCGAg                                <  7:282338/148‑1 (MQ=255)
                                                                                                                    ccTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTGCGCAGCCATTGATGTCTACGGCTTTCTGCAGCAGGCTCGTGCGCTGCTGTGGGTCGAGGTc                             <  3:282734/149‑1 (MQ=255)
                                                                                                                            gCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTGCGCAGCCATTGATGTCTACGGCTTTCTGCAGCAGGCTCGTGCGCTGCTGTGGGTCGAGGTc                             <  7:87633/141‑1 (MQ=255)
                                                                                                                            gCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTGCGCAGCCATTGATGTCTACGGCTTTCTGCAGCAGGCTCGTGCGCTGCTGTGGGTCGAGGTc                             >  8:87633/1‑141 (MQ=255)
                                                                                                                              cAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTGCGCAGCCATTGATGTCTACGGCTTTCTGCAGCAGGCTCGTGCGCTGCTGTGGGTCGAGGTCGCCAAGCAgg                   >  3:225006/1‑149 (MQ=255)
                                                                                                                               aaCTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTGCGCAGCCATTGATGTCTACGGCTTTCTGCAGCAGGCTCGTGCGCTGCTGTGGGTCGAGGTCGCCAAGCAGGc                  >  5:285096/1‑149 (MQ=255)
                                                                                                                               aaCTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTGCGCAGCCATTGATGTCTACGGCTTTCTGCAGCAGGCTCGTGCGCTGCTGTGGGTCGAGGTCGCCAAGCAGGc                  >  4:194349/1‑149 (MQ=255)
                                                                                                                                aCTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTGCGCAGCCATTGATGTCTACGGCTTTCTGCAGCAGGCTCGTGCGCTGCTGTGGGTCGAGGTCGCCAAGCAGGCg                 <  8:59358/149‑1 (MQ=255)
                                                                                                                                 cTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTGCGCAGCCATTGATGTCTACGGCTTTCTGCAGCAGGCTCGTGCGCTGCTGTGGGTCGAGGTCGCCAAGCAGGCgg                >  2:274621/1‑149 (MQ=255)
                                                                                                                                    acaacaGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTGCGCAGCCATTGATGTCTACGGCTTTCTGCAGCAGGCTCGTGCGCTGCTGTGGGTCGAGGTCGCCAAGCAGGCGGTac             >  8:328883/1‑149 (MQ=255)
                                                                                                                                      ttcggggCCGCCTTGCGGCCCTGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTGCGCAGCCATTGATGTCTACGGCTTTCTGCAGCAGGCTCGTGCGCTGCTGTGGGTCGAGGTCGCCAAGCAGGCGGTacac           >  1:357157/5‑149 (MQ=255)
                                                                                                                                      aacaGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTGCGCAGCCATTGATGTCTACGGCTTTCTGCAGCAGGCTCGTGCGCTGCTGTGGGTCGAGGTCGCCAAGCAGGCGGTacac           >  1:247544/1‑149 (MQ=255)
                                                                                                                                         aGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTGCGCAGCCATTGATGTCTACGGCTTTCTGCAGCAGGCTCGTGCGCTGCTGTGGGTCGAGGTCGCCAAGCAGGCGGTACACCTc        >  6:324002/1‑149 (MQ=255)
                                                                                                                                             ccGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTGCGCAGCCATTGATGTCTACGGCTTTCTGCAGCAGGCTCGTGCGCTGCTGTGGGTCGAGGTCGCCAAGCAGGCGGTACACCTCGGcc    <  6:67481/149‑1 (MQ=255)
                                                                                                                                               gCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTGCGCAGCCATTGATGTCTACGGCTTTCTGCAGCAGGCTCGTGCGCTGCTGTGGGTCGAGGTCGCCAAGCAGGCGGTACACCTCGGCCTg  <  8:299498/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CTTGGCGGGCGATGTGGGGCATGATGGCTGCATGACGGCTACGAGGCAGGCCAGCATGACAGACGGGGCAGGGGATGGCTTGAACGATCTTGCTGGTCGAGGCGTGTTCGTCTCAACCTCAGTGGCCAACTGACAACAGGGCCGCAAGGCGGCCCCGAATGACCCTGGGTTATGGGCGGCTGGTCAGCAGCTGTTGCGCCGTTGCGCAGCCATTGATGTCTACGGCTTTCTGCAGCAGGCTCGTGCGCTGCTGTGGGTCGAGGTCGCCAAGCAGGCGGTACACCTCGGCCTG  >  NC_002947/2724570‑2724861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑