Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,743,768 T→C 7.1% Y45H (TAT→CAT)  PP_2398 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,743,7680TC 7.1% 162.1 / 2.3 56Y45H (TAT→CAT) PP_2398hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (26/26);  new base C (2/2);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

TTGGAGAACTTTTATGTTTCCTGCGATGTTGAACGTTGGCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCTTGAGCAGACCCGGCAGTTTCTCGACCGATACCA  >  NC_002947/2743623‑2743901
                                                                                                                                                  |                                                                                                                                     
tttgagaACTTTTATGTTTCCTGCGATGTTGAACGTTGGCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATTGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTa                                                                                                                                      <  6:29783/144‑1 (MQ=255)
  ggtgaACTTTTATGTTTCCTGCGAGGGTGAACGTTGGCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGAAGTGGACTTTCGCGAGGGGGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTAt                                                                                                                                     <  7:105815/143‑1 (MQ=255)
  ggAGAACTTTTATGTTTGCTGCGATGTTGAACGTTGTACTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCG‑CCGGAACACTGGCTTGCCGTGGGCGGCGCCTAt                                                                                                                                     >  8:105815/1‑146 (MQ=255)
  ggAGAACTTTTATGTTTCCTGCGATGTTGAACGTTGGCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATgg                                                                                                                                   >  3:162897/1‑148 (MQ=255)
        cTTTTATGTTTCCTGCGATGTTGAACGTTGGCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCATAGGCGCCGCCCACGGCaatcca                                                                                                                            <  7:88942/149‑5 (MQ=255)
           ttATGTTTCCTGCGATGTTGAACGTTGGCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCGTGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTg                                                                                                                         >  5:259794/1‑149 (MQ=255)
              tGTTTCCTGCGATGTTGAACGTTGGCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGcc                                                                                                                      <  7:177461/149‑1 (MQ=255)
                tttCCTGCGATGTTGAACGTTGGCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGcc                                                                                                                      >  6:322254/1‑147 (MQ=255)
                  tCCTGCGATGTTGAACGTTGGCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCATAGGCGCCGCCCACGGCaatc                                                                                                                              >  4:318435/1‑135 (MQ=255)
                  tCCTGCGATGTTGAACGTTGGCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCATAGGCGCCGCCCACGGCaatc                                                                                                                              <  3:318435/137‑3 (MQ=255)
                     tGCGATGTTGAACGTTGGCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTgg                                                                                                               >  4:194532/1‑149 (MQ=255)
                            ttGAACGTTGGCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAgc                                                                                                         <  2:314222/148‑1 (MQ=255)
                            ttGAAAGTTGTCGTAGGGTTGCGTCGCGGCCTGTTGCCGGAACTGCTGTCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAgg                                                                                                         <  6:176318/148‑2 (MQ=255)
                                       ccTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCaa                                                                                                                                >  4:159523/1‑114 (MQ=255)
                                       ccTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCaa                                                                                                                                <  3:159523/114‑1 (MQ=255)
                                       ccTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCa                                                                                             >  7:178617/1‑149 (MQ=255)
                                        cTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCAc                                                                                            >  3:2025/1‑149 (MQ=255)
                                             cTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGt                                                                                        >  7:199600/1‑148 (MQ=255)
                                                 cGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATg                                                                                    <  8:178617/148‑1 (MQ=255)
                                                  gTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATggg                                                                                  <  3:342829/149‑1 (MQ=255)
                                                             ttGCCGGAACTGCTGGCCGTGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTa                                                                         <  6:259794/147‑1 (MQ=255)
                                                             ttGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTTGACTTTCTGGATTGTGCGCCCGGAAAACTGGATTGCCGTGGGCGGCGCCTAt                                                                                                                                     <  1:37534/88‑1 (MQ=255)
                                                             ttGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTAt                                                                                                                                     >  2:37534/1‑87 (MQ=255)
                                                                ccGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCt                                                                    <  7:233644/149‑1 (MQ=255)
                                                                ccGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCt                                                                    >  6:163681/1‑149 (MQ=255)
                                                                  ggAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTgg                                                                  >  2:244404/1‑149 (MQ=255)
                                                                   gAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTggg                                                                 >  6:365328/1‑149 (MQ=255)
                                                                           ggCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCA‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAgcg                                                                                                        <  2:230100/102‑1 (MQ=255)
                                                                           ggCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCA‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAgcg                                                                                                        >  1:230100/1‑102 (MQ=255)
                                                                               aTGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGcccc                                                     >  6:127393/1‑149 (MQ=255)
                                                                                  gAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCt                                                  >  5:325185/1‑149 (MQ=255)
                                                                                       gggTGCAGTGGACTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGAcc                                             <  7:324542/149‑1 (MQ=255)
                                                                                          tGCAGTGGACTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGAc                                          <  6:325185/149‑1 (MQ=255)
                                                                                          tGCAGTGGACTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGAc                                          <  3:194532/149‑1 (MQ=255)
                                                                                               tGGACTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTc                                     >  4:65325/1‑149 (MQ=255)
                                                                                               tGGACTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTc                                     >  4:60502/1‑149 (MQ=255)
                                                                                                 gACTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCt                                   <  8:199600/149‑1 (MQ=255)
                                                                                                   cTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCtt                                  <  8:99367/148‑1 (MQ=255)
                                                                                                   cTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCtt                                  >  7:99367/1‑148 (MQ=255)
                                                                                                   cTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCTTg                                 <  7:205086/149‑1 (MQ=255)
                                                                                                     ttCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCTTGAg                               >  7:272632/1‑149 (MQ=255)
                                                                                                      tCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGGCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCTTGAg                               <  4:162897/148‑1 (MQ=255)
                                                                                                        tGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCTTGAg                               >  2:156905/1‑146 (MQ=255)
                                                                                                          gAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCTTGAGCAGAc                          >  5:267743/1‑149 (MQ=255)
                                                                                                          gAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCTTGAGCAGAc                          >  7:173599/1‑149 (MQ=255)
                                                                                                               tgCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCTTGAGCAGACCCGGc                     <  5:127393/149‑1 (MQ=255)
                                                                                                                     cGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCTTGAGCAGACCCGGCAGttt                >  4:246566/1‑149 (MQ=255)
                                                                                                                     cGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCGGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCTTGAGCAGACCCGGCAGttt                >  3:169259/1‑149 (MQ=255)
                                                                                                                      ggAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCTTGAGCAGACCCGGCAGTTtc               <  3:60502/149‑1 (MQ=255)
                                                                                                                      ggAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCTTGAGCAGACCCGGCAGTTtc               <  3:65325/149‑1 (MQ=255)
                                                                                                                      ggAAAACTGGATTGCCGGGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCTTGAGCAGACCCGGCAGTTtc               <  8:223889/149‑1 (MQ=255)
                                                                                                                       gAAAACTGGATTGCCGTGGGCGGCGCCCATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCTTGAGCAGACCCGGCAGTTtct              >  8:291388/1‑149 (MQ=255)
                                                                                                                        aaaaCTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCTTGAGCAGACCCGGCAGTTtctc             <  3:246566/149‑1 (MQ=255)
                                                                                                                          aaCTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCTTGAGCAGACCCGGCAGTTTCTCGa           <  8:173599/149‑1 (MQ=255)
                                                                                                                                 ttGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACGTTTCCTTGAGCAGACCCGGCAGTTTCTCGACCGATAc    >  7:170761/1‑149 (MQ=255)
                                                                                                                                    ccGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCTTGAGCAGACCCGGCAGTTTCTCGACCGATACCa  <  8:272632/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                     
TTGGAGAACTTTTATGTTTCCTGCGATGTTGAACGTTGGCCTAGGCTTGCGTCGCGGCCTGTTGCCGGAACTGCTGGCCATGGAGGCGGGTGCAGTGGACTTTCTGGAGTGTGCG‑CCGGAAAACTGGATTGCCGTGGGCGGCGCCTATGGCAAGGGCTTGGCCCAGTTGGCGGAGCGCTTTGCTGTCACCTGTCATGGGTTGTCTTTATCGCTGGGTGGCAGTGCCCCGCTGGACCGACATTTCCTTGAGCAGACCCGGCAGTTTCTCGACCGATACCA  >  NC_002947/2743623‑2743901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑