Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,746,461 G→A 8.1% intergenic (‑149/+21) PP_2401 ← / ← PP_2402 membrane protein/sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,746,4610GA 8.1% 134.0 / 2.2 50intergenic (‑149/+21)PP_2401/PP_2402membrane protein/sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base G (26/19);  new base A (2/2);  total (28/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-02

AGGGACTCCCGGGGAGTGGTTGTGTGACCGGGAGTGTCTCGAGGCGGGGTATCTGCAGTGTGTCAGGGGGATGCAGTTGTGTATCTGTTTGTAGAGGCGACCGCGCGTTTTACAGGAACGGCCCGATCAAGACACAAGGCCGTTCCCGCCAACGATCGCAGAAGCCATTCAGGTGAGTTCGATCTGCGCGCACAATCCGCCCTGTTCGCGGTTGCTCAGGGTCAGCCGGCCCCCCATGGCCCGGATCAGCTGGTGGGCAATGGCCAGGCCCAAGCCGGTGCCACCCGTGCTGCGA  >  NC_002947/2746314‑2746608
                                                                                                                                                   |                                                                                                                                                   
aGGGACTCCCGGGGAGTGGTTGTGTGACCGGGAGTGTCTCGAGGCGGGGTATCTGCAGTGTGTCAGGGGGATGCAGTTGTGTATCTGTTTGTAGAGGCGACCGCGCGTTTTACAGGAACGGCCCGATCAAGACACAAGGCCGTTCCCGc                                                                                                                                                    <  1:264862/149‑1 (MQ=255)
        ccGGGGAGTGGTTGTGTGACCGGGAGTGTCTCGAGGCGGGGTATCTGCAGTGTGTCAGGGGGATGCAGTTGTGTATCTGTTTGTAGAGGCGACCGCGCGTTTTACAGGAAAGGACCGATCAAGAAACAAGGACGTTCCCGCCAACGATc                                                                                                                                            >  8:272982/1‑149 (MQ=255)
                tGGTTGTGTTACCGGGAGTGTCTCGAGGCGGGGTATGTGCAGTGTGTCAGGGGGATGCAGTTGTGTATCTGTTTGTAGAGGCGACCGCGCGTTTTACAGGAACGGCCCGATCAAGACACAAGGCCGTTCCCGCCAACGATCGCAGAAg                                                                                                                                     <  7:272982/148‑1 (MQ=255)
                  gTTGTGTGACCGGGAGTGTCTCGAGGCGGGGTATCTGCAGTGTGTCAGGCGGATGCAGTTGTGTATCTGTTTGTAGAGGCGACCGCGCTTTTTACAGGAACGGCCCGATCAAGACACAAGGCCGTACCAGCCAACGATCGCACAAGCCa                                                                                                                                  >  3:105791/1‑149 (MQ=255)
                         gACCGGGAGTGTCTCGAGGCGGGGTATCTGCAGTGTGTCAGGGGGATGCAGTTGTGTATCTGTTTGTAGAGGCGACCGCGCGTTTTACAGGAACGGCCCGATCAAGACACAAGGCCGTTCCCACCAACGATCGCAGAAGCCATTCAGGt                                                                                                                           >  1:336437/1‑149 (MQ=255)
                                aGTGTCTCGAGGCGGGGTATCTGCAGTGTGTCAGGGGGATGCAGTTGTGTATCTGTTTGTAGAGGCGACCGCGCGTTTTACAGGAACGGCCCGATCAAGACACAAGGCCGTTCCCGCCAACGATCGCAGAAGCCATTCAGGTGAGTTCg                                                                                                                    <  7:315887/149‑1 (MQ=255)
                                  tgtCTCGAGGGGGGGTATCTGCTGTGTGTCAGGGGGATGCAGTTGTGTTTCTGTTTGTAGAGGCGACCGCGAGTTTTACAGGTACGGCCTGATCAAGTCACAAGGCCTTTCCCTCCAACGATCGCAGAAGCCATTCAGGTGAGTTCGa                                                                                                                   <  4:105791/148‑1 (MQ=255)
                                        gAGGCGGGGTATCTGCAGTGTGTCAGGGGGATGCAGTTGTGTATCTGTTTGTAGAGGCGACCGCGCGTTTTACAGGAACGGCCCGATCAAGACACAAGGCCGTTCCCGCCAACGATCGCAGAAGCCATTCAGGTGAGTTCGATCTgcgc                                                                                                            >  1:283915/1‑149 (MQ=255)
                                           gCGGGGTATCTGCAGTGTGTCAGGGGGATGCAGTTGTGTATCTGTTTGTAGAGGCGACCGCGCGTTTTACAGGAACGGCCCGATCAAGACACAAGGCCGTTCCCGCCAACGATCGCa                                                                                                                                         >  8:220449/1‑117 (MQ=255)
                                           gCGGGGTATCTGCAGTGTGTCAGGGGGATGCAGTTGTGTATCTGTTTGTAGAGGCGACCGCGCGTTTTACAGGAACGGCCCGATCAAGACACAAGGCCGTTCCCGCCAACGATCGCa                                                                                                                                         <  7:214995/117‑1 (MQ=255)
                                           gCGGGGTATCTGCAGTGTGTCAGGGGGATGCAGTTGTGTATCTGTTTGTAGAGGCGACCGCGCGTTTTACAGGAACGGCCCGATCAAGACACAAGGCCGTTCCCGCCAACGATCGCa                                                                                                                                         <  7:220449/117‑1 (MQ=255)
                                           gCGGGGTATCTGCAGTGTGTCAGGGGGATGCAGTTGTGTATCTGTTTGTAGAGGCGACCGCGCGTTTTACAGGAACGGCCCGATCAAGACACAAGGCCGTTCCCGCCAACGATCGCa                                                                                                                                         >  8:214995/1‑117 (MQ=255)
                                                     tGCAGTGTGTCAGGGGGATGCAGTTGTGTATCTGTTTGTAGAGGCGACCGCGCGTTTTACAGGAACGGCCCGATCAAGACACAAGGCCGTTCCCGCCa                                                                                                                                                  >  4:54079/1‑98 (MQ=255)
                                                     tGCAGTGTGTCAGGGGGATGCAGTTGTGTATCTGTTTGTAGAGGCGACCGCGCGTTTTACAGGAACGGCCCGATCAAGACACAAGGCCGTTCCCGCCa                                                                                                                                                  <  3:54079/98‑1 (MQ=255)
                                                      gCAGTGTGTCAGGGGGATGCAGTTGTGTATCTGTTTGTAGAGGCGACCGCGCGTTTTACAGGAACGGACCGATCAAGACACAAGGCCGTTCCCGCCAACGATCGCAGAAGCCATTCAGGTGAGTTCGATCTGCGCGCACAATCCGCACt                                                                                              >  3:198505/1‑149 (MQ=255)
                                                       cAGTGTGGCAGGGGGATTCTGATGTGTATCTGTTTGTAGAGGCGACCGCGCGTTTTACAGGAACGGCCCGATCAAGACACAAGGCCGTTCCCGCCAAGGATCGCAGAAGCCATTCAGGTGAGTTCTATCTGCGCGCACAATCCGcc                                                                                                <  2:283915/146‑1 (MQ=255)
                                                               cAGGGGGATGCAGTTGTGTATCTGTTTGTAGAGGCGACCGCGCGTTTTACAGGAACGGCCCGATCAAGACACAAGGCCGTTCCCGCCAACGATCGCAGAAGCCATTCAGGt                                                                                                                           <  1:158021/111‑1 (MQ=255)
                                                               cAGGGGGATGCAGTTGTGTATCTGTTTGTAGAGGCGACCGCGCGTTTTACAGGAACGGCCCGATCAAGACACAAGGCCGTTCCCGCCAACGATCGCAGAAGCCATTCAGGt                                                                                                                           >  2:158021/1‑111 (MQ=255)
                                                                aGGGGGATGCAGTTGTGTATCTGTTTGTAGAGGCGACCGCGCGTTTTACAGGAACGGCCCGATCAAGACACAAGGCCGTTCCCGCCAACGATCGCAGAAGCCATTCAGGTGAGTTCGATCTGCGCGCACAATCCGCCCTGTTCGCGGtt                                                                                    >  5:28843/1‑149 (MQ=255)
                                                                aGGGGGATGCAGTTGTGTATCTGTTTGTAGAGGCGACCGCGCGTTTTACAGGAACGGCCCGATCAAGACACAAGGCCGTTCCCGCCAACGATCGCAGAAGCCATTCAGGTGAGTTCGATCTGCGCGCACAATCCGCCCTGTTCGCGGt                                                                                     <  5:147600/148‑1 (MQ=255)
                                                                      aTGCAGTTGTGTATCTGTTTGTAGAGGCGACCGCGCGTTTTACAGGAACGGCCCGATCAAGACACAAGGCCGTTCCCGCCAACGATCGCAGAAGCCATTCAGGTGAGTTCGATCTGCGCGCACAATCCGCCCTGTTCGCGGTTGCTCAg                                                                              <  4:183437/149‑1 (MQ=255)
                                                                               tgtATCTGTTTGTAGAGGCGACCGCGCGTTTTACAGGAACGGCCCGATCAAGACACAAGGCCGTTCCCGCCAACGATCGCAGAAGCCATTCAGGTGAGTTCGATCTGCGCGCACAATCCGCCCTGTTCGCGGTTGCTCAGGGTCAGCCg                                                                     >  7:56592/1‑149 (MQ=255)
                                                                                   tCTGTTTGTAGAGGCGACCGCGCGTTTTACAGGAACGGCCCGATCAAGACACAAGGCCGTTCCCGCCAACGATCGCAGAAGCCATTCAGGTGAGTTCGATCTGCGCGCACAATCCGCCCTGTTcgc                                                                                        <  3:281341/126‑1 (MQ=255)
                                                                                   tCTGTTTGTAGAGGCGACCGCGCGTTTTACAGGAACGGCCCGATCAAGACACAAGGCCGTTCCCGCCAACGATCGCAGAAGCCATTCAGGTGAGTTCGATCTGCGCGCACAATCCGCCCTGTTcgc                                                                                        >  4:281341/1‑126 (MQ=255)
                                                                                   tCTGTTTGTAGAGGCGACCGCGCGTTTTACAGGAACGGCCCGATCAAGACACAAGGCCGTTCCCGCCAACGATCGCAGAAGCCATTCAGGTGAGTTCGATCTGCGCGCACAATCCGCCCTGTTCGCGGTTGCTCAGGGTCAGCCGGccc                                                                 >  3:8002/1‑149 (MQ=255)
                                                                                   tCTGTTTGTAGAGGCGACCGCGCGTTTTACAGGAACGGCCCGATCAAGACACAAGGCCGTTCCCGCCAACGATCGCAGAAGCCATTCAGGTGAGTTCGATCTGCGCGCACAATCCGCCCTGTTCGCGGTTGCTCAGGGTCAGCCGGccc                                                                 >  2:246955/1‑149 (MQ=255)
                                                                                   tCTGTTTGTAGAGGCGACCGCGCGTTTTACAGGAACGGCCCGATCAAGACACAAGGCCGTTCCCACCAACGATCGCAGAAGCCATTCAGGTGAGTTCGATCTGCGCGCACAATCCGCCCTGTTCGCgg                                                                                      <  8:19937/128‑1 (MQ=255)
                                                                                   tCTGTTTGTAGAGGCGACCGCGCGTTTTACAGGAACGGCCCGATCAAGACACAAGGCCGTTCACACCAACGATCGCAGAAGCCATTCAGGTGAGTTCGATCTGCGCGCACAATCCGCCCTGTTCGCgg                                                                                      >  7:19937/1‑128 (MQ=255)
                                                                                       tttGTAGAGGCGACCGCGCGTTTTACAGGAACGGCCCGATCAAGACACAAGGCCGTTCCCGCCAACGATCGCAGAAGCCATTCAGGTGAGTTCGATCTGCGCGCACAATCCGCCCTGTTCGCGGTTGCTCAGGGTCAGCCGGCCCCCCa                                                             <  6:28843/149‑1 (MQ=255)
                                                                                                  gACCGCGCGTTTTACAGGAACGGCCCGATCAAGACACAAGGCCGTTCCCGCCAACGATCGCAGAAGCCATTCAGGTGAGTTCGATCTGCGCGCACAATCCGCCCTGTTCGCGGTTGCTCAGGGTCAGCCGGCCCCCCATGGCCCGGAt                                                   >  2:92698/1‑148 (MQ=255)
                                                                                                      gcgcgTTTTACAGGAACGGCCCGATCACGACACAAGGCCGTTCCCGCCAACGATCGCAGAAGCCATTCAGGTGAGTTCGATATGCGCGCACAATCCGCCCTGTTCGCGGTTGCTCAGGGACAGCCGGCCCCCCATGGCCCGGAACAGCt                                              >  4:74627/1‑149 (MQ=255)
                                                                                                          gTTTTACAGGAACGGCCCGATCAAGACACAAGGCCGTTCCCGCCAACGATCGCAGAAGCCATTCAGGTGAGTTCGATCTGCGCGCACAATCCGCCCTGTTCGCGGTTGCTCAGGGTCAGCCGGCCCCCCATGGCCCGGATCAGCtggt                                           <  4:198505/148‑1 (MQ=255)
                                                                                                              tACAGGAACGGCCCGATCAAGACACAAGGCCGTTCCCGCCAACGAACGCAGAAGCCATTCAGGTGAGTTCGATCTGCGCGCACAATCCGCCCTGTTCGCGGTTGCTc                                                                                >  5:333790/1‑107 (MQ=255)
                                                                                                              tACAGGAACGGCCCGATCAAGACACAAGGCCGTTCCCGCCAACGAACGCAGAAGCCATTCAGGTGAGTTCGATCTGCGCGCACAATCCGCCCTGTTCGCGGTTGCTc                                                                                <  6:333790/107‑1 (MQ=255)
                                                                                                               aCAGGAACGGCCCGATCAAGACACAAGGCCGTTCCCGCCAACGATCGCAGAAGCCATTCAGGTGAGTTCGATCTGCGCGCACAATCCGCCCTGTTCGCGGTTGCTCAGGGTCAGCCGGCCCCCCATGGCCCGGATCAGCTGGTGGGCaa                                     >  4:341721/1‑149 (MQ=255)
                                                                                                                cAGGAACGGCCCGATCAAGACACAAGGCCGTTCCCGCCAACGATCGCAGAAGCCATTCAGGTGAGTTCGATCTGCGCGCACAATCCGCCCTGTTCGCGGTTGCTCAGGGTCAGACGGCCCCCCATGGCCCGGATCAGCTGGTGGGCAAt                                    >  1:218173/1‑149 (MQ=255)
                                                                                                                         cccGATCAAGACACAAGGCCGTTCCCGCCAACGATCGCAGAAGCCATTCAGGTGAGTTCGATCTGCGCGCACAATCCGCCCTGTTCGCGGTTGCTCAGGGTCAGCCGGCCCCCCATGGCCCGGa                                                    <  4:191771/124‑1 (MQ=255)
                                                                                                                         cccGATCAAGACACAAGGCCGTTCCCGCCAACGATCGCAGAAGCCATTCAGGTGAGTTCGATCTGCGCGCACAATCCGCCCTGTTCGCGGTTGCTCAGGGTCAGCCGGCCCCCCATGGCCCGGa                                                    >  3:191771/1‑124 (MQ=255)
                                                                                                                         cccGATCAAGACACAAGGCCGTTCCCGCCAACGATCGCAGAAGCCATTCAGGTGAGTTCGATCTGCGCGCACAATCCGCCCTGTTCGCGGTTGCTCAGGGTCAGCCGGCCCCCCATGGCCCGGATCAGCTGGTGGGCAATGGCCAGGcc                           >  1:90181/1‑149 (MQ=255)
                                                                                                                          ccGATCAAGACACAAGGCCGTTCCCGCCAACGATCGCAGAAGCCATTCAGGTGAGTTCGATCTGCGCGCACAATCCGCCCTGTTCGCGGTTGCTCAGGGTCAGCCGGCCCCCCATGGCCCGGATCAGCTCGTGGGAAATGCCCAGGcc                           >  4:54087/1‑148 (MQ=255)
                                                                                                                                aaGACACAAGGCCGTTCCCGCCAACGATCGCAGAAGCCATTCAGGTGAGTTCGATCTGCGCGCACAATCCGCCCTGTTCGCGGTTGCTCAGGGTCAGCCGGCCCCCCATGGCCCGGATCAGCTGGTGGGCAATGGCCAGGCCCAAGcc                     <  8:56592/148‑1 (MQ=255)
                                                                                                                                    cacaAGGCCGTTCCCGCCAACGATCGCAGAAGCCATTCAGGTGAGTTCGATCTGCGCGCACAATCCGCCCTGTTCGCGGTTGCTCAGGGTCAGCCGGCCCCCCATg                                                           >  6:240360/1‑106 (MQ=255)
                                                                                                                                    cacaAGGCCGTTCCCGCCAACGATCGCAGAAGCCATTCAGGTGAGTTCGATCTGCGCGCACAATCCGCCCTGTTCGCGGTTGCTCAGGGTCAGCCGGCCCCCCATg                                                           <  5:240360/106‑1 (MQ=255)
                                                                                                                                     acaAGGCCGTTCCCGCCAACGATCGCAGAAGCCATTCAGGTGAGTTCGATCTGCGCGCACAATCCGCCCTGTTCGCGGTTGCTCAGGGTCAGCCGGCCCCCCATGGCCCGGATCAGCTGGTGGGCAATGGCCAGGCCCAAGCCGGTGc                >  2:53753/1‑148 (MQ=255)
                                                                                                                                     acaAGGCCGTTCCCGCCAACGATCGCAGAAGCCATTCAGGTGAGTTCGATCTGCGCGCACAATCCGCCCTGTTCGCGGTTGCTCAGGGTCAGCCGGCCCCCCATGGCCCGGATCAGCTGGTGGGCAATGGCCAGGCCCAAGCCGGTGc                >  1:140015/1‑148 (MQ=255)
                                                                                                                                              ttCCCACCAACGATCGCAGAAGCCATTCAGGTGAGTTCGATCTGCGCGCACAATCCGCCCTGTTCGCGGTTGCTCAGGGTCAGACGGCCCCCCATGGCCCGGATCAGCTGGTGGGCAATGGCCAGGCCCAAGCCGGTGCCACCCGtgct      <  2:336437/149‑1 (MQ=255)
                                                                                                                                                 ccGCCAACGATCGCAGAAGCCATTCAGGTGAGTTCGATCTGCGCGCACAATCCGCCCTGTTCGCGGTTGCTCAGGGTCAGCCGGCCCCCCATGGCCCGGATCAGCTGGTGGGCAATGGCCAGGCCCAAGCCGGTGCCACCCGtgctgc    <  3:223547/148‑1 (MQ=255)
                                                                                                                                                 ccGCCAACGATCGCAGAAGCCATTCAGGTGAGTTCGATCTGCGCGCACAATCCGCCCTGTTCGCGGTTGCTCAGGGTCAGCCGGCCCCCCATGGCCCGGATCAGCTGGTGGGCAATGGCCAGGCCCAAGCCGGTGCCACCCGtgctgc    <  2:90181/148‑1 (MQ=255)
                                                                                                                                                 ccGCCAACGATCGCAGAAGCCATTCAGGTGAGTTCGATCTGCGCGCACAATCCGCCCTGTTCGCGGTTGCTCAGGGTCAGCCGGCCCCCCATGGCCCGGATCAGCTGGTGGGCAATGGCCAGGCCCAAGCCGGTGCCACCCGtgctgc    >  7:99965/1‑148 (MQ=255)
                                                                                                                                                  cGCCAACGATCGCAGAAGCCATTCAGGTGAGTTCGATCTGCGCGCACAATCCGCCCTGTTCGCGGTTGCTCAGGGTCAGCCGGCCCCCCATGGCCCGGATCAGCTGGTGGGCAATGGCCAGGCCCAAGCCGGTGCCACCCGTGCTGCGa  >  4:174336/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AGGGACTCCCGGGGAGTGGTTGTGTGACCGGGAGTGTCTCGAGGCGGGGTATCTGCAGTGTGTCAGGGGGATGCAGTTGTGTATCTGTTTGTAGAGGCGACCGCGCGTTTTACAGGAACGGCCCGATCAAGACACAAGGCCGTTCCCGCCAACGATCGCAGAAGCCATTCAGGTGAGTTCGATCTGCGCGCACAATCCGCCCTGTTCGCGGTTGCTCAGGGTCAGCCGGCCCCCCATGGCCCGGATCAGCTGGTGGGCAATGGCCAGGCCCAAGCCGGTGCCACCCGTGCTGCGA  >  NC_002947/2746314‑2746608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑