Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	226,659	T→C	7.6%	intergenic (+66/‑67)	PP_0174 → / → PP_0175	hypothetical protein/transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	226,659	0	T	C	7.6%	185.0 / 3.7	65	intergenic (+66/‑67)	PP_0174/PP_0175	hypothetical protein/transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (32/28);  new base C (2/3);  total (34/31)
Fisher's exact test for biased strand distribution p-value = 6.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGGCGGGACCGGGCGGTGCAGATGGGCAGCACATTGGCCTTGACCATGAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTCAGCAGTGGCCTGGTAGTCGCTGCCCGCCACTGGCGGCGCATCTGTC  >  NC_002947/226518‑226801                                                                                                                                               |                                                                                                                                                gTGGCGGGACCGGGCGGTGCAGATGGGCAGCACATTGGCCTTGACCATGAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTgacga                                                                                                                                            >  4:330991/1‑147 (MQ=255)    gCGGGACCGGGCGGTGCAGATGGGCAGCACATTGGCCTTGACCATGAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAggg                                                                                                                                       >  3:99830/1‑149 (MQ=255)     cGGGACCGGTCGGTGCAGATGGGCAGCACATTGGCCTTGAACATGAAGCTGGAAGACCAGATCCATCCCTTAGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTCGTCAACAgggg                                                                                                                                      <  5:194004/149‑1 (MQ=255)     cGGGACCGGGCGGTGCAGATGGGCAGCACATTGGCCTTGACCATGAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTCGTCAACAgggg                                                                                                                                      <  5:307135/149‑1 (MQ=255)              gCGGTGCAGATGGGCAGCACATTGGCCTTGACCATGAAGCTGGACGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCggg                                                                                                                             <  7:121637/149‑1 (MQ=255)              gCGGTGCAGATGGGCAGCACATTGGCCTTGACCATGAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCggg                                                                                                                             <  3:330991/149‑1 (MQ=255)                 gTGCAGATGGGCAGCACATTGGCCTTGACCATGAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCg                                                                                                                          >  6:272689/1‑149 (MQ=255)                       aTGGGCAGCACATTGGCCTTGACCATGAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAggg                                                                                                                                       >  6:97389/1‑130 (MQ=255)                       aTGGGCAGCACATTGGCCTTGACCATGAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAggg                                                                                                                                       <  5:97389/130‑1 (MQ=255)                           gcagcaCATTGGCCTTGACCATGAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGACCAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGa                                                                                                                  >  6:157645/1‑147 (MQ=255)                           gcagcaCATTGGCCTTGACCATGAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGa                                                                                                                  >  8:186085/1‑147 (MQ=255)                           gcagcaCATTGGCCTTGACCATGAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATa                                                                                                                <  4:99830/149‑1 (MQ=255)                               cacaTTGGCCTTGACCATGAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTa                                                                                                            <  5:279236/149‑1 (MQ=255)                                acaTTGGCCTTGACCATGAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAg                                                                                                           >  4:320368/1‑149 (MQ=255)                                  aTTGGCCTTGACCATGAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGc                                                                                                          >  5:22214/1‑148 (MQ=255)                                      gCCTTGACCATGAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAAc                                                                                                     >  6:10282/1‑148 (MQ=255)                                        cTTGACCATGAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTAt                                                                                                  <  3:246099/149‑1 (MQ=255)                                            aCCATGAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGaa                                                                                               <  1:352395/148‑1 (MQ=255)                                              cATGAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTCATAGTTTGC‑TAACTATCAata                                                                                             >  3:234345/1‑148 (MQ=255)                                                    gCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTCATAGTTTGC‑TAACTATCAATATAcgccc                                                                                      <  4:234345/149‑5 (MQ=39)                                                      tGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAATATACTTGGt                                                                                     >  1:359284/1‑148 (MQ=255)                                                        gAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAATATACTTGGtt                                                                                    <  2:359284/147‑1 (MQ=255)                                                        gAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAATATACTTGGTtc                                                                                   >  2:158345/1‑148 (MQ=255)                                                           gACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAATATACTTGGTTCTCtt                                                                               <  5:10282/149‑1 (MQ=255)                                                              cAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGtt                                                                                                             <  6:174690/117‑1 (MQ=255)                                                              cAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGtt                                                                                                             >  5:174690/1‑117 (MQ=255)                                                              cAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAATATACTTGGTTCTCTTCAt                                                                            >  2:151868/1‑149 (MQ=255)                                                                gATCCATCCCTGAGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTgacga                                                                                                                                            <  3:297093/84‑1 (MQ=255)                                                                gATCCATCCCTGAGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTgacga                                                                                                                                            >  4:297093/1‑84 (MQ=255)                                                                gATCCATCCCTGAGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAATATACTTGGTTCTCTTCATGt                                                                          <  1:158345/149‑1 (MQ=255)                                                                   ccatccCTGAGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAATATACTTGGTTCTCTTCATGTccc                                                                       >  3:179580/1‑149 (MQ=255)                                                                      tccCTGAGTATTTCTCTTCAGTGCTGACCTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAATATACTTGGTTCTCTTCATGTCCCtt                                                                     >  1:199027/1‑149 (MQ=255)                                                                         cTGAGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGAtt                                                                 >  5:197609/1‑149 (MQ=255)                                                                         cTGAGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCAGTCGCCCCCTGTTGACGACAGGGGGATGTAggg                                                                                                                             >  5:346631/1‑90 (MQ=255)                                                                            aGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTcc                                                              >  6:104332/1‑149 (MQ=255)                                                                            aGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTcc                                                              <  7:186085/149‑1 (MQ=255)                                                                                tttCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTgc                                                           <  5:104332/148‑1 (MQ=255)                                                                                 ttCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAat                                                                                              >  7:143026/1‑112 (MQ=255)                                                                                 ttCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAat                                                                                              <  8:143026/112‑1 (MQ=255)                                                                                    tctTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAatat                                                                                            >  8:134234/1‑111 (MQ=255)                                                                                    tctTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAatat                                                                                            <  7:134234/111‑1 (MQ=255)                                                                                      ttCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTa                                                                                                   <  4:33905/102‑1 (MQ=255)                                                                                      ttCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTa                                                                                                   >  3:33905/1‑102 (MQ=255)                                                                                           tGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGc                                                     <  4:353105/143‑1 (MQ=255)                                                                                           tGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGc                                                     >  3:353105/1‑143 (MQ=255)                                                                                                a‑ctgaAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTcagca                                            <  6:22214/147‑1 (MQ=255)                                                                                                   tgaAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCgg                                                                                                                              >  5:65605/1‑64 (MQ=255)                                                                                                   tgaAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCgg                                                                                                                              <  6:65605/64‑1 (MQ=255)                                                                                                     aaaaaTGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTCAGCAGTGGc                                       <  4:179580/148‑1 (MQ=255)                                                                                                       aaaTGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCaa                                                   <  3:346095/134‑1 (MQ=255)                                                                                                       aaaTGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCaa                                                   >  4:346095/1‑134 (MQ=255)                                                                                                             acaacaTTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTgc                                                           <  4:191005/120‑1 (MQ=255)                                                                                                             acaacaTTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTgc                                                           >  3:191005/1‑120 (MQ=255)                                                                                                              caacaTTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTCAGCAGTGGCCTGGTAGTCg                             <  5:272689/149‑1 (MQ=255)                                                                                                               aacaTTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTCAGCAGTGGCCTGGTAGTCGc                            >  6:145549/1‑149 (MQ=255)                                                                                                                acaTTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTCAGCAGTGGCCTGGTAGTCGCt                           >  3:49678/1‑149 (MQ=255)                                                                                                                  aTTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTCAGCAGTGGCCTGGTAGTCGCTGc                         >  1:303316/1‑149 (MQ=255)                                                                                                                         cAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTCAGCAGTGGCCTGGTAGTCGCTGCCCGCCAc                  >  2:230751/1‑149 (MQ=255)                                                                                                                         cAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTCAGCAGTGGCCTGGTAGTCGCTGCCCGCCAc                  <  3:320368/149‑1 (MQ=255)                                                                                                                                 cgacatcccccTGTCGTCAACAGGGGGATGTCGGGGCGTATATTGATAGTTAGCGAAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTCAGAAGTGGCCTGGTAGTCGCTGCCCGCCACTggcggc           >  1:275809/7‑149 (MQ=255)                                                                                                                                  gacatcccccTGTCGTCAACAGGGGGCTGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTCAGCAGTGGCCTGGTAGTCGCTGCCCGCCACTGgcggcg          >  4:169733/6‑148 (MQ=255)                                                                                                                                  cGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTCAGCAGTGGCCTGGTAGTCGCTGCCCGCCACTGGCGgcgc         <  1:151868/149‑1 (MQ=255)                                                                                                                                         cccTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTCAGCAGTGGCCTGGTAGTCGCTGCCCGCCACTGGCGGCGCATCTGt   >  2:285490/1‑148 (MQ=255)                                                                                                                                         cccTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTCAGCAGTGGCCTGGTAGTCGCTGCCCGCCACTGGCGGCGCATCTGTc  <  2:199027/149‑1 (MQ=255)                                                                                                                                         cccTGTCGTCAACAGGGGGCTGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTCAGCAGTGGCCTGGTAGTCGCTGCCCGCCACTGGCGGCGCATCTGTc  <  3:169733/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                GTGGCGGGACCGGGCGGTGCAGATGGGCAGCACATTGGCCTTGACCATGAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGA‑CTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGC‑AAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTCAGCAGTGGCCTGGTAGTCGCTGCCCGCCACTGGCGGCGCATCTGTC  >  NC_002947/226518‑226801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑