Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,762,976 T→G 7.1% Q312P (CAG→CCG)  PP_2417 ← iron ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,762,9760TG 7.1% 176.7 / 4.9 56Q312P (CAG→CCG) PP_2417iron ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (25/27);  new base G (2/2);  total (27/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.84e-01

GGCCAGTTGCAGCCCCAGCCCATGGCAGGCCAGCGGAGGGATGCGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCACCGCCCAGTGCGCTGGCGGGCAACAGACGGCTG‑TGCCCGGGGCCGAGCAGAAGGCGCAAGGCATGGGGGATAACCAGCCCGACAAAACCGATG  >  NC_002947/2762829‑2763122
                                                                                                                                                   |                                                                                                                                                    
ggCCAGTTGCAGCCCCAGCCCATGGCAGGCCAGCGGAGGGATGCGAACAGCTGCCATGGCGGTCATGGGCACCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTg                                                                                                                                                     >  7:10983/1‑149 (MQ=255)
    aGTTGCAGCCCCAGCCCATGGCAGGCCAGCGGAGGGATGCGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCg                                                                                                                                                 >  1:268138/1‑149 (MQ=255)
     gTTGCAGCCCCAGCCCATGGCAGGCCAGCGGAGGGATGCGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCgg                                                                                                                                                <  1:40009/149‑1 (MQ=255)
     gTTGCAGCCCCAGCCCATGGCAGGCCAGCGGAGGGATGCGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCgg                                                                                                                                                >  2:40009/1‑149 (MQ=255)
        gCAGCCCCAGCCCATGGCAGGCCAGCGGAGGGATGCGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGa                                                                                                                                             >  8:14156/1‑149 (MQ=255)
        gCAGCCCCAGCCCATGGCAGGCCAGCGGAGGGATGCGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGa                                                                                                                                             >  8:220984/1‑149 (MQ=255)
           gccccagcccATGGCAGGCCAGCGGAGGGATGCGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCa                                                                                                                                          <  3:341564/149‑1 (MQ=255)
           gccccagcccATGGCAGGCCAGCGGAGGGATGCGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCGGCCAGGTGATcc                                                                                                                                          <  5:187549/149‑2 (MQ=255)
           gccccagcccATGGCAGGCCAGCGGAGGGATGCGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCGGCCAGGTGATcc                                                                                                                                          <  3:274581/149‑2 (MQ=255)
           gccccagcccATGGCAGGCCAGCGGAGGGATGCGAACAGCTGCCATGGCGGTCATGGGCACCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCa                                                                                                                                          <  8:10983/149‑1 (MQ=255)
             cccagcccATGGCAGGCCAGCGGAGGGATGCGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGc                                                                                                                                        >  1:208399/1‑149 (MQ=255)
             cccagcccATGGCAGGCCAGCGGAGGGATGCGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGc                                                                                                                                        >  7:8223/1‑149 (MQ=255)
              ccagcccATGGCAGGCCAGCGGAGGGATGCGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCg                                                                                                                                       >  3:292345/1‑149 (MQ=255)
                        gCAGGCCAGCGGAGGGATGCGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGaaa                                                                                                                              <  4:18416/148‑1 (MQ=255)
                                 cGGAGGGATGCGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGc                                                                                                                    <  7:220984/149‑1 (MQ=255)
                                      ggATGCGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACaa                                                                                                               <  7:289868/149‑1 (MQ=255)
                                         tGCGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGTCCGGTGATCAGCGTGCGCGAAAGAATGTATGCCACCAt                                                                                                              >  8:238485/1‑147 (MQ=255)
                                         tGCGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCa                                                                                                            <  4:127716/149‑1 (MQ=255)
                                         tGCGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCa                                                                                                            <  2:208399/149‑1 (MQ=255)
                                                gccTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAg                                                                                                    <  6:291481/147‑1 (MQ=255)
                                                      cATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCAcc                                                                                              >  3:228764/1‑149 (MQ=255)
                                                        tGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCACCGc                                                                                            >  4:351623/1‑149 (MQ=255)
                                                           cGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCACCGCCCa                                                                                         <  2:268138/149‑1 (MQ=255)
                                                            ggTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCACCGCCCAg                                                                                        >  2:248337/1‑149 (MQ=255)
                                                            ggTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCACCGCCCAg                                                                                        >  5:308215/1‑149 (MQ=255)
                                                                    gcgcCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCACTGGGCGGTGCGCTgt                                                                                <  7:14156/149‑2 (MQ=255)
                                                                        ccccGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCACCGCCCAGTGCGCTGGCggg                                                                            >  2:251820/1‑149 (MQ=255)
                                                                          ccGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCACCGCCCAGTGCGCTGGCg                                                                              <  7:61154/145‑1 (MQ=255)
                                                                          ccGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCACCGCCCAGTGCGCTGGCg                                                                              >  8:61154/1‑145 (MQ=255)
                                                                          ccGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCACCGCCCAGTGCGCTGGCGGGc                                                                           >  4:283531/1‑148 (MQ=255)
                                                                            gACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCACCGCCCATTGCGCTGGCg                                                                              <  2:97455/143‑1 (MQ=255)
                                                                            gACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCACCGCCCAGTGCGCTGGCg                                                                              >  1:97455/1‑143 (MQ=255)
                                                                            gACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCACCGCCCAGTGCGCTGGCGGGCaa                                                                         >  5:117047/1‑148 (MQ=255)
                                                                            gACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCACCGCCCAGTGCGCTGGCGGGCAAc                                                                        <  4:292345/149‑1 (MQ=255)
                                                                                      cAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCACCGCCCAGTGCGCTGGCGGGCAACAGACGGCtg‑t                                                             >  7:188946/1‑149 (MQ=255)
                                                                                       aGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGGCAACAGCGCACCGCCCAGTGCGCTGGCGGGC‑ACAGCCGTCtg‑tt                                                            <  1:251820/149‑2 (MQ=255)
                                                                                          aTCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCACCGCCCAGTGCGCTGGCGGGC‑ACAGCCGTCtg‑ttgccc                                                        <  7:279648/149‑6 (MQ=255)
                                                                                             aGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCACCGCCCAGTGCGCTGGCGGGC‑ACAGCCGTCTGTTGCCCgc                                                       <  4:146539/148‑2 (MQ=255)
                                                                                                  gAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCACCGCCCAGTGCGCTGGCGGGCAACAGACGGCTG‑TGCCCGGGGcc                                                   >  8:253737/1‑147 (MQ=255)
                                                                                                  gAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCACCGCCCAGTGCGCTGGCGGGCAACAGACGGCTG‑TGCCCGGGGCCGa                                                 <  8:8223/149‑1 (MQ=255)
                                                                                                       cGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCACCGCCCAGTGCGCTGGCGGGCAACAGACGGCTG‑TGCCCGGGGCCGAGCAg                                             <  7:253737/148‑1 (MQ=255)
                                                                                                        gggggCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCACCGCCCAGTGCGCTGGCGGGCAACAGACGGCTG‑TGCCCGGGGCCGAGCAg                                             >  6:319502/1‑147 (MQ=255)
                                                                                                          gggCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCACCGCCCAGTGCGCTGGCgg                                                                             <  6:281129/114‑1 (MQ=255)
                                                                                                          gggCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCACCGCCCAGTGCGCTGGCgg                                                                             >  5:281129/1‑114 (MQ=255)
                                                                                                             cACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCACCGCCCAGTGCGCTGGCGGGCAACAGACGGCt                                                                <  5:67791/124‑1 (MQ=255)
                                                                                                             cACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCACCGCCCAGTGCGCTGGCGGGCAACAGACGGCt                                                                >  6:67791/1‑124 (MQ=255)
                                                                                                               ccGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCACCGCCCAGTGCGCTGGCGGGCAACAGACGGCTG‑TGCCCGGGGCCGAGCAGAAGGCGCaa                                    <  3:283531/149‑1 (MQ=255)
                                                                                                                cGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCACCGCCCAGTGCGCTGGCGGGCAACAGACGGCTG‑TGCCCGGGGCCGAGCAGAAGGCGCAAg                                   >  5:237046/1‑149 (MQ=255)
                                                                                                                        gcCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCACCGCCCAGTGCGCTGGCGGGCAACAGACGGCTG‑TGCCCGGGGCCGAGCAGAAGGCGCAAGGCATgggg                           <  6:117047/149‑1 (MQ=255)
                                                                                                                              aCCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCACCGCCCAGTGCGCTGGCGGGCAACAGACGGCTG‑TGCCCGGGGCCGAGCAGAAGGCGCAAGGCATGGGGGATAAc                     <  4:228764/149‑1 (MQ=255)
                                                                                                                              aCCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCACCGCCCAGTGCGCTGGCGGGCAACAGACGGCTG‑TGCCCGGGGCCGAGCAGAAGGCGCAAGGCATGGGGGATAAc                     <  1:248337/149‑1 (MQ=255)
                                                                                                                                 aCGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCACCGCCCAGTGCGCTGGCgg                                                                             >  6:88549/1‑91 (MQ=255)
                                                                                                                                 aCGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCACCGCCCAGTGCGCTGGCgg                                                                             <  5:88549/91‑1 (MQ=255)
                                                                                                                                         gctgATCACCGGCCAGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCACCGCCCAGTGCGCTGGCGGGCAACAGACGGCTG‑TGCCCGGGGCCGAGCAGAAGGCGCAAGGCATGGGGGATAACCAGCCCGACa           >  2:341162/4‑148 (MQ=255)
                                                                                                                                           tgATCACCGGCCAGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCACCGCCCAGTGCGCTGGCGGGCAACAGACGGCTA‑TGCCCGGGGCCGAGCAGAAGGCGCAAGGCATGGGGGATAACCAGCCCGACaaaa        >  1:285991/2‑149 (MQ=255)
                                                                                                                                                 ccTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCACCGCCCAGTGCGCTGGCGGGCAACAGACGGCTG‑TGCCCGGGGCCGAGCAGAAGGCGCAAGGCATGGGGGATAACCAGCCCGACAAAACCGATg  <  6:237046/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GGCCAGTTGCAGCCCCAGCCCATGGCAGGCCAGCGGAGGGATGCGAACAGCTGCCATGGCGGTCATGGGCGCCCCCGACGGCTGACCAGAATCAGTGCGAATACGGGGGCACCGATCAGCGCCGTAACCACGCCCACCGGGATCACCTGGCCGGTGATCAGCGTGCGCGAAAGAATGTCTGCCACAATCAGG‑AACAGCGCACCGCCCAGTGCGCTGGCGGGCAACAGACGGCTG‑TGCCCGGGGCCGAGCAGAAGGCGCAAGGCATGGGGGATAACCAGCCCGACAAAACCGATG  >  NC_002947/2762829‑2763122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑