Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,824,259 T→A 12.7% L112Q (CTG→CAG)  iorA‑I → isoquinoline 1‑oxidoreductase subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,824,2590TA12.7% 140.8 / 10.8 55L112Q (CTG→CAG) iorA‑Iisoquinoline 1‑oxidoreductase subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (26/22);  new base A (4/3);  total (30/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.26e-01

CCGTGGCGGGCCGCGCTATCACCACCATTGAGGCCATCGGTGAGACGGTCGTGGGCCAAGCCGTGCAGCAGGCCTGGCTGGCGCATGAGGTGGTGCAATGCGGTTACTGCCAATCAGGGCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACACCCGGATTCGCACGGCCATTCACAGTGTTGGCCAGGTTGAGGAGGCGTGAGATGAAAAAGC  >  NC_002947/2824115‑2824397
                                                                                                                                                |                                                                                                                                          
ccgTGGCGGGCCGCGCTATCACCACCATTGAGGCCATCGGTGAGACGGTCGTGGGCCAAGCCGTGCAGCAGGCCTGGCTGGCGCATGAGGTGGTGCAATGCGGTTACTGCCAATCAGGGCAGATCATGGCCGCCACGGCGCTGCTGCAg                                                                                                                                        >  7:13080/1‑149 (MQ=255)
   tGGCGGGCCGCGCTATCACCACCATTGAGGCCATCGGTGAGACGGTCGTGGGCCAAGCCGTGCAGCAGGCCTGGCTGGCGCATGAGGTGGTGCAATGCGGTTACTGCCAATCAGGGCAGATCATGGCCGCCACGGCGCTGCTGCAgcgc                                                                                                                                     <  1:326505/149‑1 (MQ=255)
                     ccaccaTTGAGGCCATCGGTGAGACGGTCGTGGGCCAAGCCGTGCAGCAGGCCTGGCTGGCGCATGAGGTGGTGCAATGCGGTTACTGCCAATCAGGGCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAg                                                                                                                                  >  1:221989/1‑134 (MQ=255)
                     ccaccaTTGAGGCCATCGGTGAGACGGTCGTGGGCCAAGCCGTGCAGCAGGCCTGGCTGGCGCATGAGGTGGTGCAATGCGGTTACTGCCAATCAGGGCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAg                                                                                                                                  <  2:221989/134‑1 (MQ=255)
                          aTTGAGGCCATCGGTGAGACGGTCGTGGGCCAAGCCGTGCAGCAGGCCTGGCTGGCGCATGAGGTGGTGCAATGCGGTTACTGCCAATCAGGGCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGc                                                                                                              <  5:38328/149‑1 (MQ=255)
                              aGGCCATCGGTGAGACGGTCGTGGGCCAAGCCGTGCAGCAGGCCTGGCTGGCGCATGAGGTGGTGCAATGCGGTTACTGCCAATCAGGGCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAAtt                                                                                                          <  4:312900/149‑1 (MQ=255)
                              aGGCCATCGGTGAGACGGTCGTGGGCCAAGACGTGCAGCAGGCCTGGCTGGCGCATGAGGTGGTGCAATGCGGTAACTGCCAATCAGGGCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGCTAAGCCAAtt                                                                                                          >  4:206550/1‑149 (MQ=255)
                               ggCCATCGGTGAGACGGTCGTGGGCCAAGCCGTGCAGCAGGCCTGGCTGGCGCATGAGGTGGTGCAATGCGGTTACTGCCAATCAGGGCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGa                                                                                                                              <  3:334580/128‑1 (MQ=255)
                               ggCCATCGGTGAGACGGTCGTGGGCCAAGCCGTGCAGCAGGCCTGGCTGGCGCATGAGGTGGTGCAATGCGGTTACTGCCAATCAGGGCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGa                                                                                                                              >  4:334580/1‑128 (MQ=255)
                                   aTCGGTGAGACGGTCGTGGGCCAAGCCGTGCAGCAGGCCTGGCTGGCGCATGAGGTGGTGCAATGCGGTTACTGCCAATCAGGGCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGAcgc                                                                                                     >  6:278828/1‑149 (MQ=255)
                                       gTGAGACGGTCGTGGGCCAAGCCGTGCAGCAGGCCTGGCTGGCGCATGAGGTGGTGCAATGCGGTTACTGCCAATCAGGGCAGATCATGGCCGCCACGGCgctgct                                                                                                                                            <  4:289322/106‑1 (MQ=255)
                                       gTGAGACGGTCGTGGGCCAAGCCGTGCAGCAGGCCTGGCTGGCGCATGAGGTGGTGCAATGCGGTTACTGCCAATCAGGGCAGATCATGGCCGCCACGGCgctgct                                                                                                                                            >  3:289322/1‑106 (MQ=255)
                                       gTGAGACGGTCGTGGGCCAAGCCGTGCAGCAGGCCTGGCTGGCGCATGAGGTGGTGCAATGCGGTTACTGCCAATCAGGGCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGAcgccgc                                                                                                  >  6:49775/1‑148 (MQ=255)
                                           gaCGGTCGTGGGCCAAGCCGTGCAGCAGGCCTGGCTGGCGCATGAGGTGGTGCAATGCGGTTACTGCCAATCAGGGCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATgg                                                                                             <  5:49775/149‑1 (MQ=255)
                                           gaCGGTCGTGGGCCAAGCCGTGCAGCAGGCCTGGCTGGCGCATGAGGTGGTGCAATGCGGTTACTGCCAATCAGGGCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATgg                                                                                             >  3:253924/1‑149 (MQ=255)
                                            aCGGTCGTGGGCCAAGCCGTGCAGCAGGCCTGGCTGGCGCATGAGGTGGTGCAATGCGGTTACTGCCAATCAGGGCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGc                                                                                            <  3:8055/149‑1 (MQ=255)
                                                       ccAAGCCGTGCAGCAGGCCTGGCTGGCGCATGAGGTGGTGCAATGCGGTTACTGCCAATCAGGGAAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCtgt                                                                                 >  4:309163/1‑149 (MQ=255)
                                                         aaGCCGTGCAGCAGGCCTGGCTGGCGCATGAGGGGGTGCAATGCGGTTACTGCCAATCAGGGCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGc                                                                               <  3:289394/149‑1 (MQ=255)
                                                                 cagtagGCCTGGCTGGCGCATGAGGTGGTGCAATGCGGTTACTGCCAATCAGGGCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTgcgc                                                                       >  3:194048/1‑149 (MQ=255)
                                                                   gcagGCCTGGCTGGCGCATGAGGTGGTGCAATGCGGTTACTGCCAATCAGGGCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCa                                                                     >  2:348840/1‑149 (MQ=255)
                                                                    cagGCCTGGCTGGCGCATGAGGTGGTGCAATGCGGTTACTGCCAATCAGGGCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCAc                                                                    >  6:256053/1‑149 (MQ=255)
                                                                        ccTGGCTGGCGCATGAGGTGGTGCAATGCGGTTACTGCCAATCAGGGCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTac                                                                >  7:145951/1‑149 (MQ=255)
                                                                            gctggcGCATGAGGTGGTGCAATGCGGTTACTGCCAATCAGGGCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACAcc                                                             <  8:145951/148‑1 (MQ=255)
                                                                                gcgcATGAGGTGGTGCAATGCGGTTACTGCCAATCAGGGCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACACCCGGa                                                         <  8:13080/148‑1 (MQ=255)
                                                                                  gcATGAGGTGGTGCAATGCGGTTACTGCCAATCAGGGCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACACCCGGATTc                                                      >  3:78699/1‑149 (MQ=255)
                                                                                  gcATGAGGTGGTGCAATGCGGTTACTGCCAATCAGGGCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACACCCGGATTc                                                      <  4:194048/149‑1 (MQ=255)
                                                                                         gtggtgCAATGCGGTTACTGCCAATCAGGGCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACACCCGGATTCGCGCGGc                                               >  5:104275/1‑149 (MQ=255)
                                                                                             tgCAATGCGGTTACTGCCAATCAGGGCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACACCCGGATTCGCACGGCCAtt                                           <  3:206550/149‑1 (MQ=255)
                                                                                              gCAATGCGGTTACTGCCAATCAGGGCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACACCCGGATTCGCACGGCCAtt                                           >  3:219093/1‑148 (MQ=255)
                                                                                                 aTGCGGTTACTGCCAATCAGGGCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACACCCGGATTCGCGCGGCCATTcaca                                       <  6:104275/149‑1 (MQ=255)
                                                                                                    cGGTTACTGCCAATCAGGGCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACACCCg                                                           <  6:234345/126‑1 (MQ=255)
                                                                                                    cGGTTACTGCCAATCAGGGCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACACCCg                                                           >  5:234345/1‑126 (MQ=255)
                                                                                                     ggTTACTGCCAATCAGGGAAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACACCCGGATTCGCACGGCCATTCACAgtgt                                   <  3:309163/149‑1 (MQ=255)
                                                                                                      gTTACTGCCAATCAGGGCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACCCCCGGATTCGCACGGCCATTCACAGTGtt                                  >  8:274465/1‑149 (MQ=255)
                                                                                                          cTGCCAATCAGGGCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACACCCGGATTCGCACGGCCATTCACAGTGTTGGc                               <  5:278828/148‑1 (MQ=255)
                                                                                                           tGCCAATCAGGGCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACACCCGGATTCGCACGGCCATTCACAGTGTTGGc                               >  6:85914/1‑147 (MQ=255)
                                                                                                              cAATCAGGGCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACACCCGGATTCGCACGGCCATTCACAGTGTTGGCCAGGt                          >  7:24033/1‑149 (MQ=255)
                                                                                                                 tCAGGGCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACACCCGGATTCGCACGGCCATTCACAGTGTTGGCCAGGTTga                       <  4:219093/149‑1 (MQ=255)
                                                                                                                    gggCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACACCCGGATTCGCACGGCCATTCACAGTGTTGGCCAGGTTgag                      >  3:300217/1‑147 (MQ=255)
                                                                                                                     ggCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACACCCGGATTCGCACGGCCATTCACAGTGTTGGCCAGGTTga                       <  7:137412/145‑1 (MQ=255)
                                                                                                                     ggCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACACCCGGATTCGCACGGCCATTCACAGTGTTGGCCAGGTTga                       >  8:137412/1‑145 (MQ=255)
                                                                                                                      gCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACACCCGGATTCGCACGGCCATTCACAGTGTTGGCCAGGTTGaggagg                  >  4:12624/1‑149 (MQ=255)
                                                                                                                          aTCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACACCCGGATTCGCa                                                   <  5:306808/112‑1 (MQ=255)
                                                                                                                          aTCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACACCCGGATTCGCa                                                   >  6:306808/1‑112 (MQ=255)
                                                                                                                          aTCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACACCCGGATTCGCACGGCCATTCACAGTGTTGGCCAGGTTGAGGAGGCGt               >  1:218183/1‑148 (MQ=255)
                                                                                                                           tCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACACCCGGATTCGCACGGCCATTCACAGTGTTGGCCAGGTTGAGGAGGCGTga             <  4:300217/149‑1 (MQ=255)
                                                                                                                           tCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACACCCGGATTCGCACGGCCATTCACAGTGTTGGCCAGGTTGAGGAGGCGTg              <  5:85914/148‑1 (MQ=255)
                                                                                                                              tGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACACCCGGATTCGCACGGCCATTCACAGTGTTGGCCAGGTTGAGGAGGCGTgag            >  8:322532/1‑147 (MQ=255)
                                                                                                                                gccgccACGGCGCTTCTGCAGCGCCAGCCGAATCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACACCCGGATTCGCACGGCCATTCACAGTGTTGGCCAGGTTGAGGAGGCGTGAGATGa        <  8:24033/149‑1 (MQ=255)
                                                                                                                                     gctggCGCTGCAGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACACCCGGATTCGCACGGCCATTCACAgtgt                                   >  2:76377/4‑117 (MQ=255)
                                                                                                                                     gctggCGCTGCAGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACACCCGGATTCGCACGGCCATTCACAgtgt                                   <  1:76377/114‑1 (MQ=255)
                                                                                                                                     cACGGCGCTGCTGCAGCGCCAGCCAAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACACCCGGATTCGCACGGCCATTCACAGTGTTGGCCAGGTTGAGGAGGCGTGAGATGAAAAAg   >  7:226252/1‑149 (MQ=255)
                                                                                                                                      ctggCGCTGCAGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACACCCGGATTCGCACGGCCATTCACAGTGTTGGCCAGGTTGAGGAGGCGTga             >  7:84823/3‑138 (MQ=255)
                                                                                                                                      ctggCGCTGCAGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACACCCGGATTCGCACGGCCATTCACAGTGTTGGCCAGGTTGAGGAGGCGTga             <  8:84823/136‑1 (MQ=255)
                                                                                                                                      ctggCGCTGCAGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACACCCGGATTCGCACGGCCATTCACAGTGTTGGCCAGGTTGAGGAGGCGTGAGATGAAAAAGc  >  3:49253/3‑149 (MQ=255)
                                                                                                                                      ccaggcctgctgCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACACCCGGATTCGCACGGCCATTCACAGTGTTGGCCAGGTTGAGGAGGCGTGAGATGAAAAAGc  <  4:166050/143‑1 (MQ=255)
                                                                                                                                       tggCGCTGCAGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACACCCGGATTCGCACGGCCATTCACAGTGTTg                                 <  2:163326/116‑1 (MQ=255)
                                                                                                                                       tggCGCTGCAGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACACCCGGATTCGCACGGCCATTCACAGTGTTg                                 >  1:163326/2‑117 (MQ=255)
                                                                                                                                                |                                                                                                                                          
CCGTGGCGGGCCGCGCTATCACCACCATTGAGGCCATCGGTGAGACGGTCGTGGGCCAAGCCGTGCAGCAGGCCTGGCTGGCGCATGAGGTGGTGCAATGCGGTTACTGCCAATCAGGGCAGATCATGGCCGCCACGGCGCTGCTGCAGCGCCAGCCGAAGCCCGATGATCAGGCAATTGACGCCGCCATGGCGGGCAATCTGTGCCGCTGCGCCACTTACACCCGGATTCGCACGGCCATTCACAGTGTTGGCCAGGTTGAGGAGGCGTGAGATGAAAAAGC  >  NC_002947/2824115‑2824397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑