| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,858,693 | G→A | 100% | intergenic (‑49/‑77) | PP_2510 ← / → PP_2511 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,858,693 | 0 | G | A | 91.7% | 88.5 / 0.9 | 37 | intergenic (‑49/‑77) | PP_2510/PP_2511 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/3); new base A (13/20); total (14/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.03e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CAGGATCAGTTTGTTCATCGGGTGTTCCAGCAGGCTGCAAAGGGCTCGGGTGAATACGCAGTTCACCCAGCGGAAAGGCCGCGATCTTAGCAAATCCGGGTGCGGCGCTCGATCTCACCGGCAACACAAATCCATCGGCGCGCCCCCAGCCCCCCATTGCGAATTTCCTGGTTTCAGCTAACTTATACAAAAACTTGTACAAGCATGAGAGGTTGTACATGTATTGCAAGTGGCCCTTCACCCTGTACTTCGACGGCGCCTGCCCATTGT > NC_002947/2858552‑2858821 | cAGGATCAGTTTGTTCATCGGGTGTTCCAGCAGGCTGCAAAGGGCTCGGGTGAATACGCAGTTCACCCAGCGGAAAGGCCGCGATCTTAGCAAATCCGGGTGCGGCGCTCGATCTCACCGGCAACACAAATCCATCGGCGCACCCCCAg < 2:15536/149‑1 (MQ=255) aGTTTGTTCATCGGGTGTTCCAGCAGGCTGCAAAGGGCTCGGGTGAATACGCAGTTCACCCAGCGGAAAGGCCGCGATCTTAGCAAATCCGGGTGCGGCGCTCGATCTCACCGGCAACACAAATCCATCGGCGCACCCCCAGCCCCCCa < 4:308502/149‑1 (MQ=255) tCATCGGGTGTTCCAGCAGGCTGCAAAGGGCTCGGGTGAATACGCAGTTCACCCAGCGGAAAGGCCGCGATCTTAGCAAATCCGGGTGCGGCGCTCGATCTCACCGGCAACACAAATCCATCGGCGCACCCCCAGCCCCCCATTGCGaa > 6:347016/1‑149 (MQ=255) tCGGGTGTTCCAGCAGGCTGCAAAGGGCTCGGGTGAATACGCAGTTCACCCAGCGGAAAGGCCGCGATCTTAGCAAATCCGGGTGCGGCGCTCGATCTCACCGGCAACACAAATCCATCGGCGCACCCCCCCCCCCCCAATGCGAAttt > 2:579/1‑149 (MQ=255) ttCCAGCAGGCTGCAAAGGGCTCGGGTGACTACGCAGTTCACCCAGCGGAAAGGCCGCGATCTTAGCAAATCCGGGTGCGGCGCTCGATCTCACCGGCAACACAAATCCCTCGGCGCCCCCCCAGCCCCCCCTTGCGAATTTGCTgggt > 7:322671/1‑147 (MQ=255) ttCCAGCAGGCTGCAAAGGGCTCGGGTGAATACGCAGTTCACCCAGCGGAAAGGCCGCGATCTTAGCAAATCCGGGTGCGGCGCTCGATCTCACCGGCAACACAAATCCATCGGCGCACCCCCAGccc > 4:134058/1‑128 (MQ=255) ttCCAGCAGGCTGCAAAGGGCTCGGGTGAATACGCAGTTCACCCAGCGGAAAGGCCGCGATCTTAGCAAATCCGGGTGCGGCGCTCGATCTCACCGGCAACACAAATCCATCGGCGCACCCCCAGccc < 3:134058/128‑1 (MQ=255) agcagGCTGCAAAGGGCTCGGGTGAATACGCAGTTCACCCAGCGGAAAGGCCGCGATCTTAGCAAATCCGGGTGCGGCGCTCGATCTCACCGGCAACACAAATCCATCGGCGCACCCCCAGCCCCCCATTGCGAATTTCCTGGTTTCAg > 5:223630/1‑149 (MQ=255) agcagGCTGCAAAGGGCTCGGGTGAATACGCAGTTCACCCAGCGGAAAGGCCGCGATCTTAGCAAATCCGGGTGCGGCGCTCGATCTCACCGGCAACACAAATCCATCGGCGCACCCCCAGCCCCCCATTGCGAATTTCCTGGTTTCAg < 6:223630/149‑1 (MQ=255) agGCTGCAAAGGGCTCGGGTGAATACGCAGTTCACCCAGCGGAAAGGCCGCGATCTTAGCAAATCCGGGTGCGGCGCTCGATCTCACCGGCAACACAAATCCATCGGCGCACCCCCAG‑CCCCCATTGCGAATTTCCTGGTTTCAGCTaa < 6:148379/149‑1 (MQ=255) ggCTGCAAAGGGCTCGGGTGAATACGCAGTTCACCCAGCGGAAAGGCCGCGATCTTAGCAAATCCGGGTGCGGCGCTCGATCTCACCGGCAACACAAATCCATCGGCGCACCCCCAGCCCCCCATTGCGAATTTCCTGGTTTCAGCTaa < 6:196703/149‑1 (MQ=255) gCTGCAAAGGGCTCGGGTGAATACGCAGTTCACCCAGCGGAAAGGCCGCGATCTTAGCAAATCCGGGTGCGGCGCTCGATCTCACCGGCAACACAAATCCATCGGCGCACCCCCAGCCCCCCATTGCGAATTTCCTGGTTTCAGCTAAc < 5:334066/149‑1 (MQ=255) aaaGGGCTCGGGTGAATACGCAGTTCACCCAGCGGAAAGGCCGCGATCTTAGCAAATCCGGGTGCGGCGCTCGATCTCACCGGCAACACAAATCCATCGGCGCACCCCCAGCCCCCCATTGCGAATTTCCTGGTTTCAGCTAACTtata < 1:579/149‑1 (MQ=255) aaaGGGCTCGGGTGAATACGCAGTTCACCCAGCGGAAAGGCCGCGATCTTAGCAAATCCGGGTGCGGCGCTCGATCTCACCGGCAACACAAATCCATAGGCGCAGCCCCAGCGCCACATTGCGAATTTCCTGGTTTCAGCTAAATtata > 1:316165/1‑149 (MQ=255) gggTTAATACGCAGTTCTCCCAGCGTAAAGGCCGCGATCTTAGCAAATCCGGGTGCGGCGCTCGATCTCACCGGCAACACAAATCCATCGGCGCACCCCCAGCCCCCCATTGCGAATTTCCTGGTTTCAGCTAACTTATACAAAAACtt < 6:67831/149‑1 (MQ=255) gggTGAATACGCAGTTCACCCAGCGGAAAGGCCGCGATCTTAGCAAATCCGGGTGCGGCGCTCGATCTCACCGGCAACACAAATCCATCGGCGCGCCCCCATCCCCCCATTGCGAATTTCCTGGTTTCAGCTAACTTATACAAAAACtt < 3:39079/149‑1 (MQ=255) gggTGAATACGCAGTTCACCCAGCGGAAAGGCCGCGATCTTAGCAAATCCGGGTGCGGCGCTCGATCTCACCGGCAACACAAATCCATCGGCGCGCCCCCAGCCCCCCATTGCGAATTTCCTGGTTTCAGCTAACTTATACAAAAAc < 5:153434/147‑1 (MQ=255) gggTGAATACGCAGTTCACCCAGCGGAAAGGCCGCGATCTTAGCAAATCCGGGTGCGGCGCTCGATCTCACCGGCAACACAAATCCATCGGCGCACCCCCAGCCCCCCATTGCGAATTTCCTGGTTTCAGCTAACTTATACAAAAACtt > 6:24058/1‑149 (MQ=255) gTGAATACGCAGTTCACCCAGCTGAAAGGCCGCGATCTTAGCAAATCCGGGTGCGGCGATCTATCTCACCTGCAACACAAATCCATCGGCGCACCCCCAGCCCCCCATTGCGAATTTCCTGGTTTCAGCTAACTTATACAAAAACTTGt < 7:161170/149‑1 (MQ=255) tGAATACGCAGTTCACCCAGCGGAAAGGCCGCGATCTTAGCAAATCCGGGTGCGGCGCTCGATCTCACCGGCAACACAAATCCATCGGCGCACCCCCAGCCCCCCATTGCTAATTTCCTGGTTTCAGCTAACTTATACAAAAACTTGTa < 3:142093/149‑1 (MQ=255) aTACGCAGTTCACCCAGCGGAAAGGCCGCGATCTTAGCAAATCCGGGTGCGGCGCTCGATCTCACCGGCAACACAAATCCATCGGCGCACCCCCAGCCCCCCATTGCGAATTTCCTGGTTTCAGCTAACTTATACAAAAACTTGTACa < 5:24058/148‑1 (MQ=255) gCAGTTCACCCAGCGGAAAGGCCGCGATCTTAGCAAATCCGGGTGCGGCGCTCGATGTCACCGGCAACACAAATCCATCGGCGCACCCCCAGCCCCCCATTGCGAATTTCCTGGTTTCAGCGAAGTTATACAAAAACGTGTACAAGCAt > 6:163879/1‑149 (MQ=255) gCAGTTCACCCAGCGGAAAGGCCGCGATCTTAGCAAATCCGGGTGCGGCGCTCGATCTCACCGGCAACACAAATCCATCGGCGCACCCCCAGCCCCCCATTGCGAATTTCCTGGTTTCAGCTAACTTATACAAAAACTTGTACAAGCAt > 1:349535/1‑149 (MQ=255) aGTTCACCCATCGGAAAGGCCGCGATCTTAGCTATTCCGGGTGCGGCGCTCGTTCTCACCGGCAACACATTTCCATCGGCGCACCCCCAGCCCCCCATTGCGAATTTCCTGGTTTGAGGTAACTTATACAAAAACTTGTACAAGCATga < 2:316165/149‑1 (MQ=255) gTTCACCCAGCGGAAAGGCCGCGATCTTAGCAAATCCGGGTGCGGCGCTCGATCTCACCGGCAACACAAATCCATCGGCGCACCCCCAGCCCCCCATTGCGAATTTCCTGGTTTCAGCTAACTTATACAAAAACTTGt < 7:12713/138‑1 (MQ=255) gTTCACCCAGCGGAAAGGCCGCGATCTTAGCAAATCCGGGTGCGGCGCTCGATCTCACCGGCAACACAAATCCATCGGCGCACCCCCAGCCCCCCATTGCGAATTTCCTGGTTTCAGCTAACTTATACAAAAACTTGt > 8:12713/1‑138 (MQ=255) gCGGAAAGGCCGCGATCTTAGCAAATCCGGGTGCGGCGCTCGATCTCACCGGCAACACAAATCCATCGGCGCACCCCCAGCCCCCCATTGCGAATTTCCTGGTTTCAGCTAACTTATACAAAAACTTGTACAAGCATGAGAGGTTGTAc < 5:347016/149‑1 (MQ=255) aGGCCGCCATCTTAGCAAATCCGGGTGCGGCGCTCGCTCTCACCTGCAACTCAAATCCATCGGCTCACCCCCAGCCCCCCATTGCGAATTTACTGGTTTGAGCTAACTTATACTAAAACTTGTACATGCTTGAGAGGTTGTACATGTAt < 2:258646/149‑1 (MQ=255) aGCAAATCCGTGTGCGGCGCTCGATCTCACCGGCAACACAAATCCATCGGCGCACCCCCAGCCCCCCATTGCGAATTTCCTGGTTTCAGCTAACTTATACAAAAACTTGTACAAGCATGAGAGGTTGTACATGTATTGCAAGTGGccc < 5:355398/148‑1 (MQ=255) gCAAATCCGGGTGCGGCGCTCGATCTCACCGGCAACACAAATCCATCGGCGCACCCCCAGCCCCCCATTGCGAATTTACTGGTTTCAGCTAACTTATTCAAAAACTTGTACAAGAATGAGAGGTTGTACATGTATTTCAAGTGGccgtg > 8:145502/1‑146 (MQ=255) aTCCGGGTGCGGCGCTCGATCTCACAGGCAACACAAATCCATCGGCGCACCCCCCGCCCCCCATTGCGAATTTCCAGCTTTAAGCTAAGATATTCAAAAAATTGTTCAAGTATTAGATGATGTAAAATTATTGCAACTGGCCCTTAAc > 4:356536/1‑148 (MQ=255) tCGATCTCACCGGCAACACAAATCCATCGGCGCGCCCCCAGCCCCCCATTGCGAATTTCCTGGTTTCAGCTAACTTATACAAAAACTTGTACAAGCATGAGAGGTTGTACATGTATTGCTAGTGGCCCTTCACCCTGTACTTCGACGgc < 1:211620/149‑1 (MQ=255) cGATCTCCCCGGCAACCCAAATCCATCGGCCCACCCCCAGCCCCCCATTGCGAATTTCCTGGTTTCAGCTAACTTATACAAAAACTTGTACAAGCATGAGAGGTTGTACATGTATTGCAAGTGGCCCTTCACCCTGTACTTCGACGgcg < 8:322671/149‑1 (MQ=255) tcACCGGCAACACAAATCCATCGGCGCACCCCCAGCCCCCCATTGCGAATTTCCTGGTTTCAGCTAACTTATACAAAAACTTGTACAAGCATGAGAGGTTGTACATGTATTGCAAGTGGCCCTTCACCCTGTACTTCGACGGCGCCTGc > 2:63745/1‑149 (MQ=255) gCAACACAAATCCATCGGCGCACCCCCAGCCCCCCATTGCGAATTTCCTGGTTTCAGCTAACTTATACAAAAACTTGTACAAGCATGAGAGGTTGTACATGTATTGCAAGTGGCCCTTCACCCTGTACTTCGACGGCGCCTGCCCATtg < 6:177542/149‑1 (MQ=255) gCAACACAAATCCATCGGCGCACACCCAGCCCCCCATTGCGAATTTCCTGGTTTCAGCTAACTTATACAAAAACTTGTACAAGCATGAGAGGTTGTACATGTATTGCAAGTGGCCCTTCACCCTGTACTTCGACGGCGCCTGCCCATtg > 8:118548/1‑149 (MQ=255) cAACACAAATCCATCGGCGCACCCCCAGCCCCCCATTGCGAATTTCCTGGTTTCAGCTAACTTATACAAAAACTTGTACAAGCATGAGAGGTTGTACATGTATTGCAAGTGGCCCTTCACCCTGTACTTCGACGGCGCCTGCCCATtgt < 1:63745/149‑1 (MQ=255) | CAGGATCAGTTTGTTCATCGGGTGTTCCAGCAGGCTGCAAAGGGCTCGGGTGAATACGCAGTTCACCCAGCGGAAAGGCCGCGATCTTAGCAAATCCGGGTGCGGCGCTCGATCTCACCGGCAACACAAATCCATCGGCGCGCCCCCAGCCCCCCATTGCGAATTTCCTGGTTTCAGCTAACTTATACAAAAACTTGTACAAGCATGAGAGGTTGTACATGTATTGCAAGTGGCCCTTCACCCTGTACTTCGACGGCGCCTGCCCATTGT > NC_002947/2858552‑2858821 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |