Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,907,717 T→C 6.5% E326E (GAA→GAG)  hasE ← secretion metalloprotease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,907,7170TC 6.5% 175.8 / 2.1 61E326E (GAA→GAG) hasEsecretion metalloprotease
Reads supporting (aligned to +/- strand):  ref base T (28/29);  new base C (2/2);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

TTTTTACCCGGCCGGGAATGTTCGGCGTCTGCGCATGGTTGAAGGCCGGGAAGGCGATGTCCACGGCAAGGCCTGGGACCATCTTGTCGATCGCCTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTCGATCGGCGAGCGGATCACCGTGTGGGTCACTTCATAGTCCAGTGCGCGCAAGCGGTCGGCCAGGGTGGTGTTTTCCT  >  NC_002947/2907572‑2907865
                                                                                                                                                 |                                                                                                                                                    
tttttACCCGGCCGGGAATGTTCGGCGTCTGCGCATGGTTGAAGGCCGGGAAGGCGATGTCCACGGCAAGGCCTGGGACCATCTTGTCGATCGCCTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACCTc                                                                                                                                                    >  7:67549/1‑148 (MQ=255)
tttttACCCGGCCGGGAATGTTCGGCGTCTGCGCATGGTTGAAGGCCGGGAAGGCGATGTCCACGGCAAGGCCTGGGACCATCTTGTCGATCGCCTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACCTc                                                                                                                                                    >  7:72514/1‑148 (MQ=255)
    tACCCGGCCGGGAATGTTCGGCGTCTGCGCATGGTTGAAGGCCGGGAAGGCGATGTCCACGGCAAGGCCTGGGACCATCTTGTCGATCGCCTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATg                                                                                                                                                <  3:223959/148‑1 (MQ=255)
      cccGGCCGGGAATGTTCGGCGTCTGCGCATGGTTGAAGGCCGGGAAGGCGATGTCCACGGCAAGGCCTGGGACCATCTTGTCGATCGCCTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATc                                                                                                                                             <  2:260263/149‑1 (MQ=255)
       ccggccggGAATGTTCGGCGTCTGCGCATGGTTGAAGGCCGGGAAGGCGATGTCCACGGCAAGGCCTGGGACCATCTTGTCGATCGCCTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCt                                                                                                                                            >  7:64414/1‑149 (MQ=255)
        cggccggGAATGTTCGGCGTCTGCGCATGGTTGAAGGCCGGGAAGGCGATGTCCACGGCAAGGCCTGGGACCATCTTGTCGATCGCCTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACCTCCATGATCtt                                                                                                                                           <  8:72514/149‑1 (MQ=255)
        cggccggGAATGTTCGGCGTCTGCGCATGGTTGAAGGCCGGGAAGGCGATGTCCACGGCAAGGCCTGGGACCATCTTGTCGATCGCCTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACCTCCATGATCtt                                                                                                                                           <  8:67549/149‑1 (MQ=255)
         ggccggGAATGTTCGGCGTCTGCGCATGGTTGAAGGCCGGGAAGGCGATGTCCACGGCAAGGCCTGGGACCATCTTGTCGATCGCCTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTg                                                                                                                                          <  8:266266/149‑1 (MQ=255)
               gAATGTTCGGCGTCTGCGCATGGTTGAAGGCCGGGAAGGCGATGTCCACGGCAAGGCCTGGGACCATCTTGTCGATCGCCTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCg                                                                                                                                    >  2:39840/1‑149 (MQ=255)
                  tGTTCGGCGTCTGCGCATGGTTGAAGGCCGGGAAGGCGATGTCCACGGCAAGGCCTGGGACCATCTTGTCGATCGCCTGCACCGGGATCATCTCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGc                                                                                                                                 <  8:265314/149‑1 (MQ=255)
                    ttCGGCGTCTGCGCATGGTTGAAGGCCGGGAAGGCGATGTCCACGGCAAGGCCTGGGACCATCTTGTCGATCGCCTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTg                                                                                                                               <  8:138919/149‑1 (MQ=255)
                         cGTCTGCGCATGGTTGAAGGCCGGGAAGGCGATGTCCACGGCAAGGCCTGGGACCATCTTGTCGATCGCCTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGgatga                                                                                                                          <  7:116215/149‑1 (MQ=255)
                             tGCGCATGGTTGAAGGCCGGGAAGGCGATGTCCACGGCAAGGCCTGGGACCATCTTGTCGATCGCCTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATgcc                                                                                                                      >  4:21077/1‑149 (MQ=255)
                               cgcATGGTTGAAGGCCGGGAAGGCGATGTCCACGGCAAGGCCTGGGACCATCTTGTCGATCGCCTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATgcc                                                                                                                      <  8:64414/147‑1 (MQ=255)
                                gcATGGTTGAAGGCCGGGAAGGCGATGTCCACGGCAAGGCCTGGGACCATCTTGTCGATCGCCTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGAACTTGGCCCCGGGCTGGATGATgccgcc                                                                                                                   >  1:242085/1‑149 (MQ=255)
                                    ggTTGAAGGCCGGGAAGGCGATGTCCACGGCAAGGCCTGGGACCATCTTGTCGATCGCCTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGAcc                                                                                                               >  6:129237/1‑149 (MQ=255)
                                        gAAGGCCGGGAAGGCGATGTCCACGGCAAGGCCTGGGACCATCTTGTCGATCGCCTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTg                                                                                                            <  1:39840/148‑1 (MQ=255)
                                         aaGGCCGGGAAGGCGATGTCCACGGCAAGGCCTGGGACCATCTTGTCGATCGCCTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGc                                                                                                          >  8:296294/1‑149 (MQ=255)
                                                ggAAGGCGATGTCCACGGCAAGGCCTGGGACCATCTTGTCGATCGCCTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCt                                                                                                    >  2:321252/1‑148 (MQ=255)
                                                                ggCAAGGCCTGGGACCATCTTGTCGATCGCCTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGa                                                                                                                 <  3:246349/119‑1 (MQ=255)
                                                                ggCAAGGCCTGGGACCATCTTGTCGATCGCCTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGa                                                                                                                 >  4:246349/1‑119 (MQ=255)
                                                                  cAAGGCCTGGGACCATCTTGTCGATCGCCTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCg                                                                                 <  7:296294/149‑1 (MQ=255)
                                                                  cAAGGCCTGGGACCATCTTGTCGATCGCCTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCg                                                                                 >  1:1920/1‑149 (MQ=255)
                                                                    aGGCCTGGGACCATCTTGTCGATCGCCTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTc                                                                               <  3:163563/149‑1 (MQ=255)
                                                                    aGGCCTGGGACCATCTTGTCGATCGCCTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTc                                                                               <  2:278766/149‑1 (MQ=255)
                                                                      gCCTGGGACCATCTTGTCGATCGCCTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATTATCTTGGACCCGGGGTGGATATTGCCGCCCAACGTGGGAATGCTCAGGCCCTGTACCATGCCGtgg                                                                              >  8:225387/1‑146 (MQ=255)
                                                                      gCCTGGGACCATCTTGTCGATCGCCTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTCGa                                                                             >  3:11821/1‑149 (MQ=255)
                                                                                    tGTCGATCGCCTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTCGATCGGCGAGCGGAt                                                                <  3:21077/148‑1 (MQ=255)
                                                                                     gTCGATCGCCTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTCGATCGGCGAGCGGATCa                                                              >  1:364312/1‑149 (MQ=255)
                                                                                     gTCGATCGCCTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTCGATCGGCGAGCGGATCa                                                              >  1:275475/1‑149 (MQ=255)
                                                                                        gATCGCCTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTCGATCGGCGAGCGGATCACCg                                                           >  7:82141/1‑149 (MQ=255)
                                                                                        gATCGCCTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTCGATCGGCGAGCGGATCACCg                                                           >  3:300920/1‑149 (MQ=255)
                                                                                           cGCCTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTCGATCGGCGAGCGGATCACCgtgt                                                        >  3:359010/1‑149 (MQ=255)
                                                                                             ccTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTCGATCGGCGAGCGGATCACCGTGTgg                                                      <  8:82141/149‑1 (MQ=255)
                                                                                              cTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTCGATCGGCGAGCGGATCACCGTGTgg                                                      >  6:96086/1‑148 (MQ=255)
                                                                                              cTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTCGATCGGCGAGCGGATCACCGTGTgg                                                      <  5:96086/148‑1 (MQ=255)
                                                                                                 cACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTCGATCGGCGAGCGGATCACCGTGTGGGTCa                                                  >  7:287940/1‑149 (MQ=255)
                                                                                                 cACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTCGATCGGCGAGCGGATCACCGTGTGGGTCa                                                  <  7:327151/149‑1 (MQ=255)
                                                                                                      ggATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTCGATCGGCGAGCGGATCACCGTGTGGGTCACTTCa                                             <  1:321252/149‑1 (MQ=255)
                                                                                                      cgATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTCGATCGGCGAGCGGATCACCGTGTGGGTCACTTCa                                             <  6:285965/148‑1 (MQ=255)
                                                                                                           atcgcatcgACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGAGCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTCGATCGGCGAGCGGATCACCGTGTGGGTCACTTCATAGTc                                        <  2:242085/149‑1 (MQ=255)
                                                                                                            tcgcatcgACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTCGATCGGCGAGCGGATCAcc                                                            <  8:202344/128‑1 (MQ=255)
                                                                                                            tcgcatcgACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTCGATCGGCGAGCGGATCAcc                                                            >  7:202344/1‑128 (MQ=255)
                                                                                                            tcgcatcgACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTCGATCGGCGAGCGGATCACCGTGTgg                                                      <  4:40543/134‑1 (MQ=255)
                                                                                                            tcgcatcgACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTCGATCGGCGAGCGGATCACCGTGTgg                                                      >  3:40543/1‑134 (MQ=255)
                                                                                                                  cgACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTgg                                                                                                           <  3:100586/75‑1 (MQ=255)
                                                                                                                  cgACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTgg                                                                                                           >  4:100586/1‑75 (MQ=255)
                                                                                                                    aCCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTCGATCGGCGAGCGGATCACCGTGTGGGTCACTTCATAGTCCAGTgcgcg                               >  1:25827/1‑149 (MQ=255)
                                                                                                                           gCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTCGATCGGCGAGCGGATCACCGTGTGGGTCACTTCATAGTCCAGTGCGCGCAAGCgg                        <  8:287940/149‑1 (MQ=255)
                                                                                                                             ggCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTCGATCGGCGAGCGGATCACCGTGTGGGTCACTTCATAGTCCAGTGCGCGCAAGCGGTc                      <  4:11821/149‑1 (MQ=255)
                                                                                                                              gCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTCGATCGGCGAGCGGATCACCGTGTGGGTCACTTCATAGTCCAGTGCGCGCAAGCGGTCg                     >  6:201214/1‑149 (MQ=255)
                                                                                                                               cTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTCGATCGGCGAGCGGATCACCGTGTGGGTCACTTCATAGTCCAGTGCGCGCAAGCGGTCgg                    <  2:364312/149‑1 (MQ=255)
                                                                                                                                       gCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTCGATCGGCGAGCGGATCACCGTGTGGGTCACTTCATAGTCCAGTGCGCGCAAGCGGTCGGCCAGggtg            >  6:315696/1‑149 (MQ=255)
                                                                                                                                         ggTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTCGATCGGCGAGCGGATCACCGTGTGGGTCACTTCATAGTCCAGTGCGCGCAAGCGGTCGGCCAGggtggt          <  5:201214/149‑1 (MQ=255)
                                                                                                                                         ggTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTCGATCGGCGAGCGGATCACCGTGTGGGTCACTTCATAGTCCAGTGCGCGCAAGCGGTCGGCCAGggtggt          >  6:149857/1‑149 (MQ=255)
                                                                                                                                          gTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTCGATCGGCGAGCGGATCACCGTGTGGGTCACTTCATAGTCCa                                      >  8:65380/1‑120 (MQ=255)
                                                                                                                                          gTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTCGATCGGCGAGCGGATCACCGTGTGGGTCACTTCATAGTCCAGTGCGCGCAAGCGGTCGGCCAGGgtggtg         >  7:141329/1‑149 (MQ=255)
                                                                                                                                           tACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTCGATCGGCGAGCGGATCACCGTGTGGGTCACTTCATAGTCCAGTGCGCGCAAGCGGt                       <  5:194681/134‑1 (MQ=255)
                                                                                                                                           tACCACTTCCATGATATTGGCCCCGCGCTGGATGATGCCGCCGACCGTGCCGATGCTCAGGCCCTGGACCATGCCGTCGATCGGCGAGCGGATCACAGTGTGCGTAACTTCATAGTCCAGTGCGCGCAAGCCGt                       >  6:194681/1‑134 (MQ=255)
                                                                                                                                               aCTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTCGATCGGCGAGCGGATCACCGTGTGGGTCACTTCATAGTCCAGTGCGCGCAAGCGGTCGGCCAGGGTGGTGTTTTc    <  5:129237/149‑1 (MQ=255)
                                                                                                                                               aCTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTCGATCGGCGAGCGGATCACCGTGTGGGTCACTTCATAGTCCAGTGCGCGCAAGCGGTCGGCCAGGGTGGTGTTTTc    <  2:1920/149‑1 (MQ=255)
                                                                                                                                                 ttCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTCGATCGGCGAGCGGATCACCGTGTGGGTCACTTCATAGTCCAGTGCGCGCAAGCGGTCGGCCAGGGTGGTGTTTTCCt  >  7:265355/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
TTTTTACCCGGCCGGGAATGTTCGGCGTCTGCGCATGGTTGAAGGCCGGGAAGGCGATGTCCACGGCAAGGCCTGGGACCATCTTGTCGATCGCCTGCACCGGGATCATCGCATCGACCTGCAGCGGCTGGTCGAGCGGTACCACTTCCATGATCTTGGCCCCGGGCTGGATGATGCCGCCGACCGTGGCGATGCTCAGGCCCTGGACCATGCCGTCGATCGGCGAGCGGATCACCGTGTGGGTCACTTCATAGTCCAGTGCGCGCAAGCGGTCGGCCAGGGTGGTGTTTTCCT  >  NC_002947/2907572‑2907865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑