| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,909,284 | T→C | 9.6% | Q385R (CAG→CGG) ‡ | aprDA ← | alkaline protease secretion ABC transporter ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,909,284 | 0 | T | C | 9.6% | 142.3 / 3.6 | 52 | Q385R (CAG→CGG) ‡ | aprDA | alkaline protease secretion ABC transporter ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (25/22); new base C (3/2); total (28/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCCACCTCGGCAAAGCGGGCGATGTTTTCGGCGATGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGCCAGGCTGAAGCTCACCCCGCGTACGATGCTGGTGCTGCTGCCTGGGGCGCCGGCGA > NC_002947/2909140‑2909428 | tCCACCTCGGCAAAGCGGGCGATGTTTTCGGCGATGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTggg < 2:242979/148‑1 (MQ=255) ccACCTCGGCAAAGCGGGCGATGTTTTCGGCGATGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGggcc > 8:133985/1‑149 (MQ=255) aCCTCGGCAAAGCGGGCGATGTTTTCGGCGATGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCt < 2:281777/142‑1 (MQ=255) aCCTCGGCAAAGCGGGCGATGTTTTCGGCGATGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGggccgg > 4:247762/1‑149 (MQ=255) aCCTCGGCAAAGCGGGCGATGTTTTCGGCGATGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGggccgg < 7:133985/149‑1 (MQ=255) aCCTCGGCAAAGCGGGCGATGTTTTCGGCGATGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGggccg > 1:217811/1‑148 (MQ=255) aCCTCGGCAAAGCGGGCGATGTTTTCGGCGATGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGggccg < 2:217811/148‑1 (MQ=255) aCCTCGGCAAAGCGGGCGATGTTTTCGGCGATGGTGCCTTCGAACAGCTCCACGTCCAGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCt > 1:281777/1‑142 (MQ=255) ccTCGGCAAAGCGGGCGATGTTTTCGGCGATGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGggccggc < 8:209293/149‑1 (MQ=255) ccTCGGCAAAGCGGGCGATGTTTTCGGCGATGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTACAGGCGCCCCATTGCGGCCCCGggcgg > 5:79994/1‑146 (MQ=255) cTCGGCAAAGCGGGCGATGTTTTCGGCGATGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCCAggccggc > 3:246923/1‑148 (MQ=255) ggCAAAGCGGGCGATGTTTTCGGCGATGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCCAggccggcaagg < 4:246923/149‑5 (MQ=255) gCAAAGCGGGCGATGTTTTCGGCGATGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGGGCCGGCCacac > 1:278073/1‑149 (MQ=255) aGCGGGCGATGTTTTCGGCGATGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGGGCCGGCCACACGCCt < 2:264582/149‑1 (MQ=255) gCGGGCGATGTTTTCGGCGATGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGggccgg > 5:318649/1‑138 (MQ=255) gCGGGCGATGTTTTCGGCGATGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGggccgg < 6:318649/138‑1 (MQ=255) gATGTTTTCGGCGATGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGGGCCGGCCACACGCCTACCaa > 1:278389/1‑147 (MQ=255) gATGTTTTCGGCGATGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGGGCCGGCCACACGCCTACCAACa < 2:27599/149‑1 (MQ=255) gATGTTTTCGGCGATGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TGGCCGGCCCAggccggcaag > 1:11790/1‑133 (MQ=255) gATGTTTTCGGCGATGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGAGCCGTCCAGGCGCACC‑TGGCCGGCCCAggccggcaag < 2:11790/136‑4 (MQ=255) ttttCGGCGATGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGGGCCGGCCACACGCCTACCAACAggc < 5:184072/148‑1 (MQ=255) tGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATAGCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTa < 4:71600/149‑1 (MQ=255) tGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGAc > 4:223198/1‑149 (MQ=255) gCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTg < 7:346788/108‑1 (MQ=255) gCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTg > 8:346788/1‑108 (MQ=255) tCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCccgaggcc > 8:46584/1‑147 (MQ=255) cACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAgg < 8:343411/127‑1 (MQ=255) cACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAgg > 7:343411/1‑127 (MQ=255) cGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGAt < 7:46584/147‑1 (MQ=255) ggTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACAccc < 2:278073/149‑1 (MQ=255) tAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGc > 1:232244/1‑121 (MQ=255) tAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGc < 2:232244/121‑1 (MQ=255) gCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAgg > 4:1940/1‑109 (MQ=255) gCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAgg < 3:1940/109‑1 (MQ=255) gCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGc > 5:151213/1‑149 (MQ=255) aCGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCAccc < 6:151213/149‑1 (MQ=255) ggCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCggg < 3:247762/149‑1 (MQ=255) ccAGTTCGGCCTTGTTCCAGGTTAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCAGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGAAACCCAGGCTGTCACCCGGGccc > 7:229518/1‑149 (MQ=255) ccAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGc > 7:302294/1‑148 (MQ=255) ggCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGCCAGGCTg > 5:292073/1‑148 (MQ=255) gCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGCCAGGCTg > 5:362478/1‑147 (MQ=255) ccAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGCCAGGCTGAAGCTCAccc > 5:3026/1‑149 (MQ=255) aGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGAATTGCCCGAGGCCGAGGGGCCGATGACACCCAGGATGTCACCCGGGGCCAGCCTGACGCTCCCCCcg > 6:141974/1‑149 (MQ=255) ggTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGCCAGGCTGAAGCTCACCCcgc < 2:278389/149‑1 (MQ=255) aGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGCCAGGCTGAAGCTCACCCCGCGTAc > 6:344655/1‑148 (MQ=255) tatCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGa < 6:250000/99‑1 (MQ=255) tatCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGa > 5:250000/1‑99 (MQ=255) gcgcCGTCCAGGCGCACC‑TTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGCCAGGCTGAAGCTCACCCCGCGTACGATGCTGGt > 2:184600/1‑149 (MQ=255) gcCGTCCAGGCGCACC‑TTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGCCAGGCTGAAGCTCACCCCGCGTACGATGCTGGtgc < 3:223198/149‑1 (MQ=255) ccGTCCAGGCGCACC‑TTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGCCAGGCTGAAGCTCACCCCGCGTACGATGCTGGtgct > 6:60389/1‑149 (MQ=255) gTCCAGGCGCACC‑TTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGCCAGGCTGAAGCTCACCCCGCGTACGATGCTGGtgctgc > 5:212434/1‑149 (MQ=255) tCCAGGCGCACC‑TTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGCCAGGCTGAAGCTCACCCCGCGTACGATGCTGGtgctgct < 6:362478/149‑1 (MQ=255) cAGGCGCACC‑TTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGCCAGGCTGAAGCTCACCCCGCGTACGATGCTGGtgctgctgc > 5:19385/1‑149 (MQ=255) ggCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGCCAGGCTGAAGCTCACCCCGCGTACGATGCTGGTGCTGCTGCCTGGGGCGCCGGCGa < 7:200820/149‑1 (MQ=255) | TCCACCTCGGCAAAGCGGGCGATGTTTTCGGCGATGGTGCCTTCGAACAGCTCCACGTCCTGCGGCAGGTAGCCCAGCCACGGGCCCAGTTCGGCCTTGTTCCAGGTGAAGATATCGGCGCCGTCCAGGCGCACC‑TTGCCGGCCTGGGCCGGCCACACGCCTACCAACAGGCGGGCGAGGGTAGACTTGCCCGAGGCCGAGGGGCCGATGACACCCAGGCTGTCACCCGGGGCCAGGCTGAAGCTCACCCCGCGTACGATGCTGGTGCTGCTGCCTGGGGCGCCGGCGA > NC_002947/2909140‑2909428 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |