Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,985,148	G→T	6.5%	P86T (CCC→ACC)	PP_2611 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,985,148	0	G	T	6.5%	209.3 / 3.5	71	P86T (CCC→ACC)	PP_2611	membrane protein
Reads supporting (aligned to +/- strand):  ref base G (37/29);  new base T (2/3);  total (39/32)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01

ACCGCCTGCGACTTGGCTCCAGTCAAATATTATGAGTTTTTTGAATCGCTTATAGTAGATTTTCTTTGTGTGTCGATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAACATGATAATACCCAGGTAAATAGAAACATGGCAGTGTAGATTTTTCCCATGAATACAGAGTCGGACACCCAA  >  NC_002947/2985002‑2985296                                                                                                                                                   |                                                                                                                                                     aCCGCCTGCGACTTGGCTCCAGTCAAATATTATGAGTTTTTTGAATCGCTTATAGTAGATTTTCTTTGTGTGTCGATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGa                                                                                                                                                    <  8:269816/149‑1 (MQ=255)               ggCTCCAGTCAAATATTATGAGTTTTTTGAATCGCTTATAGTAGATTTTCTTTGTGTGTCGATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATg                                                                                                                                      >  3:264151/1‑149 (MQ=255)                gCTCCAGTCAAATATTATGAGTTTTTTGAATCGCTTATAGTAGATTTTCTTTGTGTGTCGATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATgg                                                                                                                                     <  5:272102/149‑1 (MQ=255)                  tCCAGTCAAATATTATGAGTTTTTTGAATCGCTTATAGTAGATTTTCTTTGTGTGTCGATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGc                                                                                                                                    >  2:335929/1‑148 (MQ=255)                  tCCAGTCAAATATTATGAGTTTTTTGAATCGCTTATAGTAGATTTTCTTTGTGTGTCGATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGc                                                                                                                                    >  1:71691/1‑148 (MQ=255)                    cAGTCAAATATTATGAGTTTTTTGAATCGCTTATAGTAGATTTTCTTTGTGTGTCGATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCaa                                                                                                                                  <  4:264151/148‑1 (MQ=255)                     aGTCAAATATTATGAGTTTTTTGAATCGCTTATAGTAGATTTTCTTTGTGTGTCGATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAAc                                                                                                                                 >  2:311169/1‑148 (MQ=255)                       tCAAATATTATGAGTTTTTTGAATCGCTTATAGTAGATTTTCTTTGTGTGTCGATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGAt                                                                                                                                       >  3:9630/1‑140 (MQ=255)                       tCAAATATTATGAGTTTTTTGAATCGCTTATAGTAGATTTTCTTTGTGTGTCGATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGAt                                                                                                                                       <  4:9630/140‑1 (MQ=255)                       tCAAATATTATGAGTTTTTTGAATCGCTTATAGTAGATTTTCTTTGTGTGTCGATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGa                                                                                                                               >  8:232094/1‑148 (MQ=255)                       tCAAATATTATGAGTTTTTTGAATCGCTTATAGTAGATTTTCTTTGTGTGTCGATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGa                                                                                                                               >  8:217895/1‑148 (MQ=255)                         aaaTATTATGAGTTTTTTGAATCGCTTATAGTAGATTTTCTTTGTGTGTCGATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGaaaa                                                                                                                            >  8:9595/1‑149 (MQ=255)                              ttatGAGTTTTTTGAATCGCTTATAGTAGATTTTCTTTGTGTGTCGATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTcg                                                                                                                       >  3:100332/1‑149 (MQ=255)                                 tGAGTTTTTTGAATCGCTTATAGTAGATTTTCTTTGTGTGTCGATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTcgcc                                                                                                                     <  2:71691/148‑1 (MQ=255)                                      tttttGAATCGCTTATAGTAGATTTTCTTTGTGTGTCGATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGtt                                                                                                               >  5:318210/1‑149 (MQ=255)                                          tGAATCGCTTATAGTAGATTTTCTTTGTGTGTCGATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGttgat                                                                                                            <  3:23515/148‑1 (MQ=255)                                           gAATCGCTTATAGTAGATTTTCTTTGTGTGTCGATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGttgatt                                                                                                           <  6:285492/148‑1 (MQ=255)                                             aTCGCTTATAGTAGATTTTCTTTGTGTGTCGATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGttgattga                                                                                                         <  6:65775/148‑1 (MQ=255)                                                gCTTATAGTAGATTTTCTTTGTGTGTCGATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGagta                                                                                                      <  6:340884/148‑1 (MQ=255)                                                      agtagATTTTCTTTGTGTGTCGATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCtata                                                                                               >  8:171664/1‑149 (MQ=255)                                                       gtagATTTTCTTTGTGTGTCGATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTatat                                                                                              >  4:297886/1‑149 (MQ=255)                                                        tagATTTTCTTTGTGTGTCGATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGttga                                                                                                             <  2:116941/133‑1 (MQ=255)                                                        tagATTTTCTTTGTGTGTCGATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGttga                                                                                                             >  1:116941/1‑133 (MQ=255)                                                        tagATTTTCTTTGTGTGTCGATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATAtt                                                                                             >  4:260898/1‑149 (MQ=255)                                                           aTTTTCTTTGTGTGTCGATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGTGa                                                                                                                                                    <  3:271401/91‑1 (MQ=255)                                                           aTTTTCTTTGTGTGTCGATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGTGa                                                                                                                                                    <  3:276402/91‑1 (MQ=255)                                                           aTTTTCTTTGTGTGTCGATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGTGa                                                                                                                                                    >  4:276402/1‑91 (MQ=255)                                                           aTTTTCTTTGTGTGTCGATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGTGa                                                                                                                                                    >  4:271401/1‑91 (MQ=255)                                                             tttCTTTGTGTGTCGATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCGGCCGTTGATTGAGTAGTCTATATTTTCAt                                                                                        >  8:327574/1‑149 (MQ=255)                                                                 tttGTGTGTCGATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTg                                                                                                                                                 >  6:294392/1‑88 (MQ=255)                                                                 tttGTGTGTCGATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTg                                                                                                                                                 <  5:294392/88‑1 (MQ=255)                                                                  ttGTGTGTCGATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAAt                                                                                    <  3:260898/148‑1 (MQ=255)                                                                    gtgtgtCGATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATga                                                                                  <  3:297886/148‑1 (MQ=255)                                                                      gtgtCGATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGAt                                                                               >  4:190050/1‑149 (MQ=255)                                                                           gATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTaa                                                                          <  6:318210/149‑1 (MQ=255)                                                                             tttAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAACa                                                                        <  7:103471/149‑1 (MQ=255)                                                                               tAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAACAt                                                                       >  1:347505/1‑148 (MQ=255)                                                                                aaaTATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAACATg                                                                      <  2:347505/148‑1 (MQ=255)                                                                                        gTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATgag                                                                                 <  8:232263/129‑1 (MQ=255)                                                                                        gTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATgag                                                                                 >  7:232263/1‑129 (MQ=255)                                                                                         ttCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAACATGATAATAcc                                                              >  1:64979/1‑147 (MQ=255)                                                                                         ttCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCAGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAAAATGATAATAcc                                                              >  2:273428/1‑147 (MQ=255)                                                                                         tacGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCAGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAACATGATAATACCCa                                                            >  7:66806/3‑149 (MQ=255)                                                                                             aTAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCta                                                                                                 <  2:210080/108‑1 (MQ=255)                                                                                              tAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAACATGATAATACCCAGGTa                                                        >  3:9306/1‑148 (MQ=255)                                                                                                 gTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGTGATTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAACATGATAATACCCAGGTAAATa                                                    <  7:232094/149‑1 (MQ=255)                                                                                                 gTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAACATGATAATACCCAGGTAAATa                                                    <  3:190050/149‑1 (MQ=255)                                                                                                  ttttttATGAAAAGTATTCTGTAGGCTAAGAATAGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGAAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTATCATGATAATACCCAGGTaaa                                                      >  7:303459/1‑146 (MQ=255)                                                                                                    ttttATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAACATGATAATACCCAGGTAAATAGa                                                  >  2:279611/1‑148 (MQ=255)                                                                                                     tttATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAACATGATAATACCCAGGTAAATAGaa                                                 <  1:335929/148‑1 (MQ=255)                                                                                                         tGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAACATGATAATACCCAGGTAAATAGAAACAt                                             <  7:217895/148‑1 (MQ=255)                                                                                                         tGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAACATGATAATACCCAGGTAAATAGAAACATg                                            >  4:102115/1‑149 (MQ=255)                                                                                                            ataGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAACATGATAATACCCAGGTAAATAGAAACATGGCa                                         <  1:311169/147‑1 (MQ=255)                                                                                                            aaaGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAACATGATAATACCCAGGTAAATAGAAACATGGCa                                         <  7:9595/149‑1 (MQ=255)                                                                                                             aaGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAACATGATAATACCCAGGTAAATAGAAACATGGAAg                                        >  1:141025/1‑149 (MQ=255)                                                                                                                tATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAACATGATAATACCCAGGTAAATAGAAACATGGCAgtg                                      >  6:338403/1‑148 (MQ=255)                                                                                                                     tGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAACATGATAATACCCAGGTAAATAGAAACATGGCAGTGTAGAtt                                >  6:266489/1‑149 (MQ=255)                                                                                                                     tGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAACATGATAATACCCAGGTAAATAGAAACATGGCAGTGTAGAtt                                >  2:284357/1‑149 (MQ=255)                                                                                                                       tAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAACATGATAATACCCAGGTAAAt                                                     >  3:64589/1‑126 (MQ=255)                                                                                                                       tAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAACATGATAATACCCAGGTAAAt                                                     <  4:64589/126‑1 (MQ=255)                                                                                                                       tAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAACATGATAATACCCAGGTAAATAGAAACATGGCAGTGTAGAtt                                <  1:284357/147‑1 (MQ=255)                                                                                                                            tAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAACATGATAATACCCAGGTAAATAGAAACATGGCAGTGTAGATTTTTCCCa                         >  6:47745/1‑149 (MQ=255)                                                                                                                            tAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAACATGATAATACCCAGGAAAATAGAAACATGGCAGTGTAGATTTTTCCCa                         >  1:358792/1‑149 (MQ=255)                                                                                                                                 aTGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAACATGATAATACCCAGGTAAATAGAAACATGGCAGTGTAGATTTTTCCCATGaa                     <  2:64979/148‑1 (MQ=255)                                                                                                                                      gTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAACATGATAATACCCAGGTAAATAGAAACATGGCAGTGTAGATTTTTCCCATGAATACaga               <  1:279611/149‑1 (MQ=255)                                                                                                                                          aaaTAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAACATGATAATACCCAGGTAAATAGAAACATGGCAGTGTAGATTTTTCCCATGAATACAGAGTCg           >  5:337115/1‑149 (MQ=255)                                                                                                                                          aaaTAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAACATGATAATACCCAGGTAAATAGAAACATGGCAGTGTAGATTTTTCCCATGAATACAGAGTCg           <  4:100332/149‑1 (MQ=255)                                                                                                                                           aaTAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAACATGATAATACCCAGGTAAATAGAAACATGGCAGTGTAGATTTTTCCCATGAATACAGAGTCg           >  2:20080/1‑148 (MQ=255)                                                                                                                                           aaTAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAACATGATAATACCCAGGTAAATAGAAACATGGCAGTGTAGATTTTTCCCATGAATACAGAGTCg           >  2:87205/1‑148 (MQ=255)                                                                                                                                              aaaGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAACATGATAATACCCAGGTAAATAGAAACATGGCAGTGTAGATTTTTCCCATGAATACAGAGTCGGaca       <  7:327574/149‑1 (MQ=255)                                                                                                                                               aaGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAACATGATAATACCCAGGTAAATAGAAACATGGCAGTGTAGATTTTTCCCATGAATACAGAGTCGGacac      >  2:347182/1‑149 (MQ=255)                                                                                                                                                 ggggAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAACATGATAATACCCAGGTAAATAGAAACATGGCAGTGTAGATTTTTCCCATGAATACAGAGTCGGa         >  5:283270/1‑144 (MQ=255)                                                                                                                                                 ggggAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAACATGATAATACCCAGGTAAATAGAAACATGGCAGTGTAGATTTTTCCCATGAATACAGAGTCGGa         <  6:283270/144‑1 (MQ=255)                                                                                                                                                   ggAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAACATGATAATACCCAGGTAAATAGAAACATGGCAGTGTAGATTTTTCCCATGAATACAGAGTCGGACACCCaa  <  7:171664/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                     ACCGCCTGCGACTTGGCTCCAGTCAAATATTATGAGTTTTTTGAATCGCTTATAGTAGATTTTCTTTGTGTGTCGATTTAAATATATGTTCGATAAGTTTTTTATGAAAAGTATTCTGTAGGCTAAGAATGGGGTAAAAATAAAGGGGAGTGAGATCAGGATGGCAACGAAAATGTCGCCGCCGTTGATTGAGTAGTCTATATTTTCATTAATGAGATTGTAACATGATAATACCCAGGTAAATAGAAACATGGCAGTGTAGATTTTTCCCATGAATACAGAGTCGGACACCCAA  >  NC_002947/2985002‑2985296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑