Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,021,733 C→A 7.8% T757T (ACC→ACA)  PP_2638 → cellulose synthase operon protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,021,7330CA 7.8% 157.5 / 3.7 51T757T (ACC→ACA) PP_2638cellulose synthase operon protein C
Reads supporting (aligned to +/- strand):  ref base C (23/24);  new base A (2/2);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

TTGCGGCGCGACGCAGCCCGCCTGGTAGCGCAGGATGATCCTCAACGAGCACTGGACCTTTACGCGGCAGCGATGGCCGATGCGCAGTTGCTGGACCGTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTGGATGCCCTGTATCGCCAGCGCAATACCCATGTGACGCTGATGCATGACTACGGCTGGCGCGAGGATGACGGTACGCCCGGTACTTCGG  >  NC_002947/3021599‑3021881
                                                                                                                                      |                                                                                                                                                    
ttgCGGCGCGACGCAGCCCGCCTGGTAGCGCAGGATTATCCTCAACGAGCACTGGACCTTTACGCGTCAGCGATGGCCGATGCGCAGTTGCTGGACCGTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACCCTGGCCAGCCGCGa                                                                                                                                        <  3:280969/149‑1 (MQ=255)
            gCAGCCCGCGTGGTAGCGGAGGATGATCCTAAAGGAGGACTGGACCTTTACGCGGCAGCGATGGCCGATGCGCAGTTGCTGGACCGTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGcga                                                                                                                            <  4:322702/149‑1 (MQ=255)
             cAGCCCGCCTGGTAGCGCAGGATGATCCTCAACGAGCACTGGACCTTTACGCGGCAGCGATGGCCGATGCGCAGTTGCTGGACCGTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGcgac                                                                                                                           <  4:187803/149‑1 (MQ=255)
                    ccTGGTAGCGCAGGATGATCCTCAACGAGCACTGGACCTTTACGCGGCAGCGATGGCCGATGCGCAGTTGCTGGACCGTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACCCTGGCCAGc                                                                                                                                             <  4:118862/124‑1 (MQ=255)
                    ccTGGTAGCGCAGGATGATCCTCAACGAGCACTGGACCTTTACGCGGCAGCGATGGCCGATGCGCAGTTGCTGGACCGTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACCCTGGCCAGc                                                                                                                                             >  3:118862/1‑124 (MQ=255)
                        gTAGCGCAGGATGATCCTCAACGAGCACTGGACCTTTACGCGGCAGCGATGGCCGATGCGCAGTTGCTGGACCGTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACtggctggc                                                                                                                >  5:107740/1‑149 (MQ=255)
                           gcgcAGGATGATCCTCAACGAGCACTGGACCTTTACGCGGCAGCGATGGCCGATGCGCAGTTGCTGGACCGTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGccc                                                                                                              <  6:107740/148‑1 (MQ=255)
                                ggATGATCCTCAACGAGCACTGGACCTTTACGCGGCAGCGATGGCCGATGCGCAGTTGCTGGACCGTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGcc                                                                                                        >  5:364481/1‑149 (MQ=255)
                                ggATGATCCTCAACGAGCACTGGACCTTTACGCGGCAGCGATGGCCGATGCGCAGTTGCTGGACCGTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGcc                                                                                                        >  2:220715/1‑149 (MQ=255)
                                   tgatCCTCAACGAGCACTGGACCTTTACGCGGCAGCGATGGCCGATGCGCAGTTGCTGGACCGTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTgc                                                                                                     >  3:91736/1‑149 (MQ=255)
                                           aaCGAGCACTGGACCTTTACGCGGCAGCGATGGCCGATGCGCAGTTGCTGGACCGTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGAt                                                                                             >  1:290011/1‑149 (MQ=255)
                                             cGAGCACTGGACCTTTACGCGGCAGCGATGGCCGATGCGCAGTTGCTGGACCGTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGAtgt                                                                                           <  3:96231/149‑1 (MQ=255)
                                              gAGCACTGGACCTTTACGCGGCAGCGATGGCCGATGCGCAGTTGCTGGACCGTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGAtgtg                                                                                          >  7:300946/1‑149 (MQ=255)
                                               aGCACTGGACCTTTACGCGGCAGCGATGGCCGATGCGCAGTTGCTGGACCGTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTgg                                                                                         <  4:91736/149‑1 (MQ=255)
                                                gCACTGGACCTTTACGCGGCAGCGATGGCCGATGCGCAGTTGCTGGACCGTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTGGa                                                                                        <  8:300946/149‑1 (MQ=255)
                                                 cACTGGACCTTTACGCGGCAGCGATGGCCGATGCGCAGTTGCTGGACCGTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACACTGGCCAGCCGCGAGCAGGATGGCGACGACtggctggc                                                                                                                >  1:115078/1‑124 (MQ=255)
                                                 cACTGGACCTTTACGCGGCAGCGATGGCCGATGCGCAGTTGCTGGACCGTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACACTGGCCAGCCGCGAGCAGGATGGCGACGACtggctggc                                                                                                                >  1:110834/1‑124 (MQ=255)
                                                 cACTGGACCTTTACGCGGCAGCGATGGCCGATGCGCAGTTGCTGGACCGTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACACTGGCCAGCCGCGAGCAGGATGGCGACGACtggctggc                                                                                                                <  2:110834/124‑1 (MQ=255)
                                                 cACTGGACCTTTACGCGGCAGCGATGGCCGATGCGCAGTTGCTGGACCGTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACACTGGCCAGCCGCGAGCAGGATGGCGACGACtggctggc                                                                                                                <  2:115078/124‑1 (MQ=255)
                                                      gACCTTTACGCGGCAGCGATGGCCGATGCGCAGTTGCTGGACCGTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTGGATGCCCt                                                                                  <  2:155014/149‑1 (MQ=255)
                                                      gACCTTTACGCGGCAGCGATGGCCGATGCGCAGTTGCTGGACCGTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTGGATGCCCt                                                                                  >  1:155014/1‑149 (MQ=255)
                                                      gACCTTTACGCGGCAGCGATGGCCGATGCGCAGTTGCTGGACCGTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTAGATGCCCt                                                                                  <  6:364481/149‑1 (MQ=255)
                                                       aCCTTTACGCGGCAGCGATGGCCGATGCGCAGTTGCTGGACCGTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTGGATGCCCTg                                                                                 <  2:290011/149‑1 (MQ=255)
                                                         cTTTACGCGGCAGCGATGGCCGATGCGCAGTTGCTGGACCGTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTGGATGCCCTGTa                                                                               >  4:93189/1‑149 (MQ=255)
                                                         cTTTACGCGGCAGCGATGGCCGATGCGCAGTTGCTGGACCGTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTGGATGCCCTGTa                                                                               >  1:203734/1‑149 (MQ=255)
                                                                           gCCGATGCGCAGTTGCTGGACCGTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTGGATGCCCTGTATCGCCAGCGCAATAccc                                                              <  5:23440/148‑1 (MQ=255)
                                                                           gCCGATGCGCAGTTGCTGGACCGTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTGGATGCCCTGTATCGCCAGCGCAATAccc                                                              <  2:203734/148‑1 (MQ=255)
                                                                            ccGATGCGCAGTTGCTGGACCGTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTGGATGCCCTGTATCGCCAGCGCAATACCCAt                                                            <  3:259229/149‑1 (MQ=255)
                                                                             cGATGCGCAGTTGCTGGACCGTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTGGATGCCCTGTATCGCCAGCGCAATACCCAtg                                                           >  2:329020/1‑149 (MQ=255)
                                                                                         gCTGGACCGTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTGGATGCCCTGTATCGCCAGCGCAATACCCATGTGACGCTGATg                                                >  2:267135/1‑148 (MQ=255)
                                                                                           tGGACCGTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTGGATGCCCTGTATCGCCAGCGCAATACCCATGTGACGCTGATGCAt                                             >  6:3482/1‑149 (MQ=255)
                                                                                            ggACCGTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTGGATGCCCTGTATCGCCAGCGCAATACCCATGTGACTCTGATGCAt                                             >  1:237184/1‑148 (MQ=255)
                                                                                             gACCGTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTGGATGCCCTGTATCGCCAGCGCAATACCCATGTGACGCTGATGCAt                                             <  4:64404/147‑1 (MQ=255)
                                                                                                 gTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTGGATGCCCTGTATCGCCAGCGCAATACCCATGTGACGCTGATGCATGa                                           >  2:286289/1‑145 (MQ=255)
                                                                                                 gTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTGGATGCCCTGTATCGCCAGCGCAATACCCATGTGACGCTGATGCATGa                                           <  1:286289/145‑1 (MQ=255)
                                                                                                         gTAGCGCCACGGGATGACCGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTGGATGCCCTGTATCGCCAGCGCAATACCCATGTGACGCTGATGCATGACTACGGCTGgcg                               >  7:96937/1‑149 (MQ=255)
                                                                                                            gcgcCACGGGAGGACCGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCTCAGCCTGCGCAGTGATGTGGATGCCCTGTATCGCCAGCGCAATACGCATGTGACGCTGATGCATGACTACGGCTGGCGCGa                            <  3:358100/149‑1 (MQ=255)
                                                                                                               ccACGGGATTACCGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTGGATGCCCt                                                                                  <  4:299346/92‑1 (MQ=255)
                                                                                                                cACGGGATGACCGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTGGATGCCCTGTATCGCCAGCGCAATACCCATGTGACGCTGATGCATGACTACGGCTGGCGCGAGGAt                        <  5:3482/149‑1 (MQ=255)
                                                                                                                        gACCGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTGGATGCCCTGTATCGCCAGCGCAATACCCATGTGACGCTGATGCATGACTACGGCTGGCGCGAGGATGa                      <  5:204699/143‑1 (MQ=255)
                                                                                                                        gACCGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTGGATGCCCTGTATCGCCAGCGCAATACCCATGTGACGCTGATGCATGACTACGGCTGGCGCGAGGATGa                      >  6:204699/1‑143 (MQ=255)
                                                                                                                           cGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTGGATGCCCTGTATCGCCAGCGCAAt                                                                  >  8:169101/1‑96 (MQ=255)
                                                                                                                           cGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTGGATGCCCTGTATCGCCAGCGCAATACCCATGTGACGCTGATGCATGACTACGGCTGGCGCGAGGATGACGGTACGcc             >  5:272134/1‑149 (MQ=255)
                                                                                                                           cGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTGGATGCCCTGTATCGCCAGCGCAATACCCATGTGACGCTGATGCATGACTACGGCTGGCGCGAGGATGACGGTACGcc             >  5:277142/1‑149 (MQ=255)
                                                                                                                            gTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTGGATGCCCTGTATCGCCAGCGCAATACCCATGTGACGCTGATGCATGACTACGGCTGGCGCGAGGATGACGGTACGccc            <  8:96937/149‑1 (MQ=255)
                                                                                                                            gTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTGGATGCCCTGTATCGCCAGCGCAATACCCATGTGACGCTGATGCATGACTACGGCTGGCGCGAGGATGACGGTACGc              >  6:124254/1‑147 (MQ=255)
                                                                                                                            gTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTGGATGCCCTGTATCGCCAGCGCAATACCCATGTGACGCTGATGCATGACTACGGCTGGCGCGAGGATGACGGTACGc              <  5:124254/147‑1 (MQ=255)
                                                                                                                             tGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGCCTGGCTGGCCCGCAGCCTGCGCAGTGATGTGGATGCCCTGTATCGCCAGCGCAATACCCATGTGACGCTGATGCATGACTACGGCTGGCGCGa                            <  4:264862/132‑1 (MQ=255)
                                                                                                                               cATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTGGATGCCCTGTATCGCCAGCGCAATACCCATGTGACGCTGATGCATGACTACGGCTGGCGCGAGGATGACGGTACGCCCgg          <  3:337509/148‑1 (MQ=255)
                                                                                                                                 ttGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTGGATGCCCTGTATCGCCAGCGCAATACCCATGTGACGCTGATGCATGACTACGGCTGGCGCGAGGATGACGGTACGCCCGGTAc       >  8:258632/1‑149 (MQ=255)
                                                                                                                                  tGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTGGATGCCCTGTATCGCCAGCGCAATACCCATGTGACGCTGATGCATGACTACGGCTGGCGCGAGGATGACGGTACGCCCGGTACt      >  3:156059/1‑149 (MQ=255)
                                                                                                                                     cccTTGCCAGGCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGGAGTGATGTGGATGCCCTGTATCGCCAGCGCAATACCCATGTGACGCTGATGCATGACTACGGCTGGCGCGAGGATGACGGTACGCCCGGTACTTCg   <  1:329020/149‑1 (MQ=255)
                                                                                                                                     cccTGGCCAGCCGAGATCAGGATGGCGACGACTGGCTGTCCCGCAGCCTGCGCAGTGATGTGGATGCCCTGTATCGCCAGCGCAATACCCATGTGACTCTGATGCATGACTACGGCTGGCGCGAGGATGACGGTACGCCCGGTACTTc    <  2:237184/148‑1 (MQ=255)
                                                                                                                                      ccTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTGGATGCCCTGTATCGCCAGCGCAATACCCATGTGACGCTGATGCATGACTACGGCTGGCGCGAGGATGACGGTACGCCCGGTACTTCgg  <  6:272134/149‑1 (MQ=255)
                                                                                                                                      ccTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTGGATGCCCTGTATCGCCAGCGCAATACCCATGTGACGCTGATGCATGACTACGGCTGGCGCGAGGATGACGGTACGCCCGGTACTTCgg  <  6:277142/149‑1 (MQ=255)
                                                                                                                                      ccTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTGGATGCCCTGTATCGCCAGCGCAATACCCATGTGACGCTGATGCATGACTACGGCTGGCGCGAGGATGACGGTACGCCCGGTACTTCgg  <  4:156059/149‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                                    
TTGCGGCGCGACGCAGCCCGCCTGGTAGCGCAGGATGATCCTCAACGAGCACTGGACCTTTACGCGGCAGCGATGGCCGATGCGCAGTTGCTGGACCGTGCTGCCGTAGCGCCACGGGATGACCGTGCATTGACCCTGGCCAGCCGCGAGCAGGATGGCGACGACTGGCTGGCCCGCAGCCTGCGCAGTGATGTGGATGCCCTGTATCGCCAGCGCAATACCCATGTGACGCTGATGCATGACTACGGCTGGCGCGAGGATGACGGTACGCCCGGTACTTCGG  >  NC_002947/3021599‑3021881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑