Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,068,885 A→T 7.7% intergenic (+136/‑27) qedH‑II → / → aldB‑II quinoprotein ethanol dehydrogenase/aldehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,068,8850AT 7.7% 152.3 / 2.7 52intergenic (+136/‑27)qedH‑II/aldB‑IIquinoprotein ethanol dehydrogenase/aldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (16/31);  new base T (2/2);  total (19/33)
Fisher's exact test for biased strand distribution p-value = 6.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

GTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAGGCTCAGCATGATCTACGCACAACCCGGAACTCCAGGCGCCGTCGTATCCTTCAAACCCCGTTATGGCAACTTCATCGGTGGCGAGTTCGTGCAGCCGTTGGCTGGCCAGTACTTCATCAACAGC  >  NC_002947/3068738‑3069028
                                                                                                                                                   |                                                                                                                                               
gTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGa                                                                                                                                                 >  5:90769/1‑148 (MQ=255)
   aaGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGTCAgg                                                                                                                                             >  1:40362/1‑149 (MQ=255)
   aaGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGTCAgg                                                                                                                                             >  1:45599/1‑149 (MQ=255)
      ccATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGACAGGCAc                                                                                                                                          <  4:31504/149‑1 (MQ=255)
      ccATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGACAGGCAc                                                                                                                                          <  6:105816/149‑1 (MQ=255)
        aTAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGACAGGCACCg                                                                                                                                        >  8:308548/1‑149 (MQ=255)
         tAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGACAGGCACCGa                                                                                                                                       <  5:328584/149‑1 (MQ=255)
            cGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGACAGGCAc                                                                                                                                          <  4:74093/143‑1 (MQ=255)
            cGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGACAGGCAc                                                                                                                                          >  3:74093/1‑143 (MQ=255)
            cGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGACAGGCACCGAccc                                                                                                                                    <  6:90769/149‑1 (MQ=255)
                  cctACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCaga                                                                                                                               <  8:344537/148‑1 (MQ=255)
                  cctACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCAGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCag                                                                                                                                <  4:218081/147‑1 (MQ=255)
                      cTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAGGCTc                                                                                                                          <  8:130893/149‑1 (MQ=255)
                          cGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAGGCTCAGCa                                                                                                                      >  5:163530/1‑149 (MQ=255)
                               gctgcCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAGGCTCAGCATGAt                                                                                                                  <  3:203880/148‑1 (MQ=255)
                                 tgcCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAGGCTCAGCATGATCTa                                                                                                               >  6:185817/1‑149 (MQ=255)
                                  gcCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAGGCTCAGCATGATCTAc                                                                                                              >  5:4543/1‑149 (MQ=255)
                                     gCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAGGCTCAGCATGATCTACGca                                                                                                           >  3:29907/1‑149 (MQ=255)
                                     gCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAGGCTCAGCATGATCTACGca                                                                                                           <  4:333332/149‑1 (MQ=255)
                                      cTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAGGCTCAGCATGATCTACGcac                                                                                                          <  4:206398/149‑1 (MQ=255)
                                            ggTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAGGCTCAGCATGATCTACGCACAACCCg                                                                                                    <  4:96375/149‑1 (MQ=255)
                                             gTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAGGCTCAGCATGATCTACGCACAACCCgg                                                                                                   <  4:104439/149‑1 (MQ=255)
                                                gCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAGGCTCAGCATGATCTACGCACAACCCGGaa                                                                                                 <  6:91546/148‑1 (MQ=255)
                                                gCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAGGCTCAGCATGATCTACGCACAACCCGGaa                                                                                                 <  6:163530/148‑1 (MQ=255)
                                                  aaGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAgg                                                                                                                             <  7:159929/118‑1 (MQ=255)
                                                  aaGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAgg                                                                                                                             >  8:159929/1‑118 (MQ=255)
                                                                ccAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAGGCTCAGCATGATCTACGCACAACCCGGAACTCCAGGCGCcgtcgt                                                                                 <  6:4543/148‑1 (MQ=255)
                                                                 cAAATGACGAGAGCCCCGCTGCCAACGATGCGTTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAGGCTCAGCATGATCTACGCACAACCCGGAACTCCAGGCGCCGTCGTAt                                                                               >  4:113273/1‑149 (MQ=255)
                                                                     tGACGAGAGCCCCGCTGCCAACGATGCTTTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAGGCTCAGCATGATCTACGCACAACCCGGAACTCCAGGCGCCGTCGTATCCtt                                                                           <  3:113273/149‑1 (MQ=255)
                                                                         gagaGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAGGCTCAGCATGATCTACGCACAACCCGGAACTCCAGGCGCCGTCGTATCCTTCaaa                                                                       >  8:91373/1‑149 (MQ=255)
                                                                             gCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGTCAGGCACCGACCCGCAGAGGCTCAGCATGATCTACGCACAACCCGGAACTCCAGGCGCCGTCGTATCCTTCAAAcccc                                                                   <  2:45599/149‑1 (MQ=255)
                                                                             gCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGTCAGGCACCGACCCGCAGAGGCTCAGCATGATCTACGCACAACCCGGAACTCCAGGCGCCGTCGTATCCTTCAAAcccc                                                                   <  2:40362/149‑1 (MQ=255)
                                                                                 cGCTGCCAACGATGCATTCGCCCGGCACACGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAGGCTCAGCATGATCTACGCACAACCCGGAACTCCAGGCGCcgtcgt                                                                                 <  8:117652/131‑1 (MQ=255)
                                                                                 cGCTGCCAACGATGCATTCGCCCGGCACACGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAGGCTCAGCATGATCTACGCACAACCCGGAACTCCAGGCGCcgtcgt                                                                                 >  7:117652/1‑131 (MQ=255)
                                                                                        aaCGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAGGCTCAGCATGATCTACGCACAACCCGGAACTCCAGGCGCCGTCGTATCCTTCAAACCCCGTTATGGCAAc                                                        >  1:52292/1‑149 (MQ=255)
                                                                                               cATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAGGCTCAGCATGATCTACGCACAACCCGGAACTCCAGGCGCCGTCGTATCCTTCAAACCCCGTTATGGCAACTTCATCg                                                 <  4:29907/149‑1 (MQ=255)
                                                                                                 ttCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGCCAGGCACCGACCCGCAGAGGCTCAGCATGATCTACGCACAACCCGGAACTCCAGGCGCCGTCGTAGCCTTCAAACCCCGTTATGGCACCTTCATCGGt                                               >  7:261584/1‑149 (MQ=255)
                                                                                                 ttCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAGGCTCAGCATGATCTACGCACAACCCGGAACTCCAGGCGCCGTCGTATCCTTCAAACCCCGTTATGGCAACTTCATCGGt                                               >  2:260248/1‑149 (MQ=255)
                                                                                                    gCCCCGCACGCGGGGCTGCTTACCCTCGGCGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAGGCTCAGCATGATCTCCGCACAACCCGCACCTCCAGGCGCCGGCGTATCCTTCACACCCCGTTATGGCACCTTCATCGGTGGc                                            <  8:261584/149‑1 (MQ=255)
                                                                                                           aCGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAGGCTCAGCATGATCTACGCACAACCCGGAACTCCAGGCGCCGTCGTATCCTTCAAACCCCGTTATGGCAACTTCATCGGTGGCGAGTTCg                                     >  7:112286/1‑149 (MQ=255)
                                                                                                             gcgGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAGGCTCAGCATGATCTACGCACAACCCGGAACTCCAGGCGCCGTCGTATCCTTCAAACCCCGTTATGGCAACTTCATCGGTGGCGAGTTCGTg                                   <  2:52292/149‑1 (MQ=255)
                                                                                                                      cttACCATCGGTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAGGCTCAGCATGATCTACGCACAACCCGGAACTCCAGGCGCCGTCGTATCCTTCAAACCCCGTTATGGCAACTTCATCGGTGGCGAGTTCGTg                                   >  1:287651/1‑140 (MQ=255)
                                                                                                                      cttACCATCGGTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAGGCTCAGCATGATCTACGCACAACCCGGAACTCCAGGCGCCGTCGTATCCTTCAAACCCCGTTATGGCAACTTCATCGGTGGCGAGTTCGTg                                   <  2:287651/140‑1 (MQ=255)
                                                                                                                         aCCATCGGTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAGGCTCAGCATGATCTACGCACAACCCGGAACTCCAGGCGCCGTCGTATCCTTCAAACCCCGTTATGGCAACTTCATCGGTGGCGAGTTCGTGCAGCCGTtgg                         <  5:185817/147‑1 (MQ=255)
                                                                                                                            aTCGGTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAGGCTCAGCATGATCTACGCACAACCCGGACCTCCAGGCGCCGTCGTATCCTTCAAACCCCGTTATGGCAACTTCATCGGTGGCGAGTTCGTGCAGCCGTtggctggc                    <  7:308548/149‑1 (MQ=255)
                                                                                                                            aTCGGTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAGGCTCAGCATGATCTACGCACAACCCGGAACTCCAGGCGCCGTCGTATCCTTCAAACCCTGTTATGGCAACTTCATCGGTGGCGAGTTCGTGCAGCCGTtggctggc                    <  5:177529/149‑1 (MQ=255)
                                                                                                                               ggTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAGGCTCAGCATGATCTACGCACAACCCGGAACTCCAGGCGCCGTCGTATCCTTCAAACCCCGTTATGGCAACTTCATCGGTGGCGAGTTCGTGCAGCCGTTGGCTGGCCAg                 >  2:276604/1‑149 (MQ=255)
                                                                                                                                gTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAGGCTCAGCATGATCTACGCACAACCCGGAACTCCAGGCGCCGTCGTATCCTTCAAACCCCGTTATGGCAACTTCATCGGTGGCGAGTTCGt                                    >  6:39043/1‑129 (MQ=255)
                                                                                                                                gTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAGGCTCAGCATGATCTACGCACAACCCGGAACTCCAGGCGCCGTCGTATCCTTCAAACCCCGTTATGGCAACTTCATCGGTGGCGAGTTCGt                                    <  5:39043/129‑1 (MQ=255)
                                                                                                                                 tGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAGGCTCAGCATGATCTACGCACAACCCGGAACTCCAGGCGCCGTCGTATCCTTCAAACCCCGTTATGGCAACTTCATCGGTGGCGAGTTCGTGCAGCCGTTGGCTGGCCAGTa               <  7:91373/149‑1 (MQ=255)
                                                                                                                                       gCCTGTCGTGGGACAGGCACCGACCCGCAGAGGCTCAGCATGATCTACGCACAACCCGGAACTCCAGGCGCCGTCGTATCCTTCAAACCCCGTTATGGCAACTTCATCGGTGGCGAGTTCGTGCAGCCGTTGGCTGGCCAGTACTtcat         <  1:276604/149‑1 (MQ=255)
                                                                                                                                        ccTGTCGTGGGACAGGCACCGACCCGCAGAGGCTCAGCATGATCTACGCACAACCCGGAACTCCAGGCGCCGTCGTATCCTTCAAACCCCGTTATGGCAACTTCATCGGTGGCGAGTTCGTGCAGCCGTTGGCTGGCCAGTACTtcatc        <  8:112286/149‑1 (MQ=255)
                                                                                                                                              gTGGGACAGGCACCGACCCGCAGAGGCTCAGCATGATCTACGCACAACCCGGAACTCCAGGCGCCGTCGTATCCTTCAAACCCCGTTATGGCAACTTCATCGGTGGCGAGTTCGTGCAGCCGTTGGCTGGCCAGTACTTCATCAACAGc  <  6:58470/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
GTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCATCGCCTGTCGTGGGACAGGCACCGACCCGCAGAGGCTCAGCATGATCTACGCACAACCCGGAACTCCAGGCGCCGTCGTATCCTTCAAACCCCGTTATGGCAACTTCATCGGTGGCGAGTTCGTGCAGCCGTTGGCTGGCCAGTACTTCATCAACAGC  >  NC_002947/3068738‑3069028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑