Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,089,376 C→G 8.0% V279V (GTG→GTC)  metE ← 5‑methyltetrahydropteroyltriglutamate‑ homocysteine methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,089,3760CG 8.0% 181.8 / 5.3 61V279V (GTG→GTC) metE5‑methyltetrahydropteroyltriglutamate‑ homocysteine methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (27/29);  new base G (2/3);  total (29/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

TTGAGCTTGCCGCTGGCTACCTGGCGGGGCAGGGGCGCCATGCCGCAGTTGGTGCAAGGGTAGAGCTTGTCGGCATCGACGAATTGCAGTGCCTTGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCGGAACATGCGAGTTATGGCATTCCAGCGAAATGATATCGATGCTGGACTGCTGCAGTTTGGGGAAGGCCTGTTCATATTGCCGCCATTCT  >  NC_002947/3089233‑3089523
                                                                                                                                               |                                                                                                                                                   
ttGAGCTTGCCGCTGGCTACCTGGCGGGGCAGGGGCGCCATGCCGCAGTTGGTGCAAGGGTAGAGCTTGTCGGCATCGACGAATTGCAGTGCCTTGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATc                                                                                                                                                >  4:306608/1‑149 (MQ=255)
  gAGCTTGCCGCTGGCTACCTGGCGGGGCAGGGGCGCCATGCCGCAGTTGGTGCAAGGGTAGAGCTTGTCGGCATCGACGAATTGCAGTGCCTTGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGa                                                                                                                                              >  4:78727/1‑149 (MQ=255)
   aGCTTGCCGCTGGCTACCTGGCGGGGCAGGGGCGCCATGCCGCAGTTGGTGCAAGGGTAGAGCTTGTCGGCATCGACGAATTGCAGTGCCTTGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGa                                                                                                                                              <  8:26300/148‑1 (MQ=255)
     cTTGCCGCTGGCTACCTGGCGGGGCAGGGGCGCCATGCCGCAGTTGGTGCAAGGGTAGAGCTTGTCGGCATCGACGAATTGCAGTGCCTTGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATg                                                                                                                                            <  4:44512/148‑1 (MQ=255)
      ttGCCGCTGGCTACCAGGAGGTGCAGGGGCGCCATGCCGAAGTTGCTGAAAGGGTAGAGCTTGTCGGCATCGACGAAATGCAGTGCCTAGCGCACGGTGGCGGCGACCTGTACGGAAGTCTCAATGACATGGGTGGCCACATCCATGgc                                                                                                                                          >  4:258750/1‑149 (MQ=255)
        gCCGCTGGCTACCTGGCGGGGCAGGGGCGCCATGCCGCAGTTGGTGCAAGGGTAGAGCTTGTCGGCATCGACGAATTGCAGTGCCTTGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGgc                                                                                                                                          <  3:203737/147‑1 (MQ=255)
         ccGCTGGCTACCTGGCGGGGCAGGGGCGCCATGCCGCAGTTGGTGCAAGGGTAGAGCTTGTCGGCATCGACGAATTGCAGTGCCTTGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGcc                                                                                                                                       >  8:68381/1‑149 (MQ=255)
                 tACCTGGCGGGGCAGGGGCGCCATGCCGCAGTTGGTGCAAGGGTAGAGGTTGTCGGCATCGAGGAATTGCGGTGCCTGGGGCAGGGTGGCGGCGAGCGGGGCGGGGGTGGCGATGAGAGGGGTGGGGAGATCGATGGCGCCGAcctggg                                                                                                                               >  4:75322/1‑145 (MQ=255)
                   ccTGGCGGGGCAGGGGCGCCATGCCGCAGTTGGTGCAAGGGTAGAGCTTGTCGGCATCGACGAATTGCAGTGCCTTGCGCAAGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACt                                                                                                                             <  6:41210/149‑1 (MQ=255)
                    cTGGCGGGGCAGGGGCGCCATGCCGCAGTTGGTGCAAGTGTAGAGCTTGTCGGCATCGACGAATTGCAGTGCCTTGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGTCGGCGCCATCGATGTGGCCACCCATgtcat                                                                                                                            <  8:262355/149‑5 (MQ=38)
                      ggCGGGGCAGGGGCGCCATGCCGCAGTTGGTGCAAGGGTAGAGCTTGTCGGCATCGACGAATTGCAGTGCCTTGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGTCGGCGCCATCGATGTGGCCACCCATgtcat                                                                                                                            <  7:233710/147‑5 (MQ=38)
                      ggCGGGGCAGGGGCGCCATGCCGCAGTTGGTGCAAGGGTAGAGCTTGTCGGCATCGACGAATTGCAGTGCCTTGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGTCGGCGCCATCGATGTGGCCACCCATgtcat                                                                                                                            >  8:233710/1‑143 (MQ=38)
                       gCGGGGCAGGGGCGCCATGCCGCAGTTGGTGCAAGGGTAGAGCTTGTCGGCATCGACGAATTGCAGTGCCTTGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGTCGGCGCCATCGATGTGGCCACCCATgtcatt                                                                                                                           <  2:105744/147‑6 (MQ=38)
                       gCGGGGCAGGGGCGCCATGCCGCAGTTGGTGCAAGGGTAGAGCTTGTCGGCATCGACGAATTGCAGTGCCTTGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACttt                                                                                                                           <  2:105313/147‑1 (MQ=255)
                        cGGGGCAGGGGCGCCATGCCGCAGTTGGTGCAAGGGTAGAGCTTGTCGGCATCGACGAATTGCAGTGCCTTGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCtt                                                                                                                        <  8:104298/149‑1 (MQ=255)
                             cAGGGGCGCCATGCCGCAGTTGGTGCAAGGGTAGAGCTTGTCGGCATCGACGAATTGCAGTGCCTTGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCcgc                                                                                                                   <  4:116520/149‑1 (MQ=255)
                                gggCGCCATGCCGCAGTTGGTGCAAGGGTAGAGCTTGTCGGCATCGACGAATTGCAGTGCCTTGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGaa                                                                                                                <  2:261080/149‑1 (MQ=255)
                                     ccATGCCGCAGTTGGTGCAAGGGTAGAGCTTGTCGGCATCGACGAATTGCAGTGCCTTGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAg                                                                                                            >  7:215198/1‑148 (MQ=255)
                                                   gTGCAAGGGTAGAGCTTGTCGGCATCGACGAATTGCAGTGCCTTGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCAt                                                                                             <  3:75322/149‑1 (MQ=255)
                                                    tGCAAGGGTAGAGCTTGTCGGCATCGACGAATTGCAGTGCCTTGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATc                                                                                            >  5:339479/1‑149 (MQ=255)
                                                      cAAGGGTAGAGCTTGTCGGCATCGACGAATTGCAGTGCCTTGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCgg                                                                                          <  2:331041/149‑1 (MQ=255)
                                                       aaGGGTAGAGCTTGTCGGCATCGACGAATTGCAGTGCCTTGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCgg                                                                                          >  7:330838/1‑148 (MQ=255)
                                                       aaGGGTAGAGCTTGTCGGCATCGACGAATTGCAGTGCCTTGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCGGa                                                                                         <  6:339479/149‑1 (MQ=255)
                                                         gggTAGAGCTTGTCGGCATCGACGAATTGCAGTGCCTTGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCGGAAc                                                                                       <  3:180567/149‑1 (MQ=255)
                                                           gTAGAGCTTGTCGGCATCGACGAATTGCAGTGCCTTGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCGGAACAt                                                                                     <  3:78727/149‑1 (MQ=255)
                                                              gagCTTGTCGGCATCGACGAATTGCAGTGCCTTGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCGGAACATGc                                                                                   <  3:306608/148‑1 (MQ=255)
                                                                gCTTGTCGGCATCGACGAATTGCAGTGCCTTGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCGGAACATGCGAg                                                                                >  5:347221/1‑149 (MQ=255)
                                                                gCTTGTCGGCATCGACGAATTGCAGTGCCTTGAGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCGGAACATGCGAg                                                                                >  5:126510/1‑149 (MQ=255)
                                                                     tCGGCATCGACGTATTGCAGTGGCTTGCTCAGGGTGGCGGCGACCTGTTCGGGTGTCTCATTTACATGGGTGGCCACATCGATGTCGCCGACCTTGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCGGAACATGCTAGTTATg                                                                           <  3:258750/149‑1 (MQ=255)
                                                                        gCATCGACGAATTGCAGTGCCTTGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCATGTCCATCGGAACATGCGAGTTATGGCa                                                                        >  1:322084/1‑149 (MQ=255)
                                                                          agcgacgaATTGCAGTGCCTTGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCGGAACATGCGAGTTATGGCAtt                                                                      <  8:330838/147‑1 (MQ=255)
                                                                          aTCGACGAATTGCAGTGCCTTGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCg                                                                                           <  5:93443/128‑1 (MQ=255)
                                                                          aTCGACGAATTGCAGTGCCTTGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCg                                                                                           >  6:93443/1‑128 (MQ=255)
                                                                             gacgaATTGCAGTGCCTTGCGCAGGGTGGCGGTGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCGGAACATGCGAGTTATGGCATTc                                                                     >  5:27110/1‑147 (MQ=255)
                                                                             gacgaATTGCAGTGCCTTGCGCAGGGTGGCGGTGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACAATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCGGAACATGCGAGTTATGGCATTc                                                                     >  5:21865/1‑147 (MQ=255)
                                                                             gacgaATTGCAGTGCCTTGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCGGAACATGCGAGTTATGGCATTc                                                                     >  4:142501/1‑147 (MQ=255)
                                                                              acgaATTGCAGTGCCTTGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCGGAACATGCGAGTTATGGCATTCCAg                                                                  <  7:68381/149‑1 (MQ=255)
                                                                                        gTGCCTTGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCGGAACATGCGAGTTATGGCATTCCAGCGAAATGata                                                        >  1:325259/1‑149 (MQ=255)
                                                                                           ccTTGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCCTCGGAACATGCGAGTTATGGCATTCCAGCGAAATGATATc                                                      >  1:179953/1‑148 (MQ=255)
                                                                                           ccTTGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCGGAACATGCGAGTTATGGCATTCCAGCGAAATGATATCg                                                     >  5:273411/1‑149 (MQ=255)
                                                                                            cTTGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCGGAACATGCGAGTTATGGCATTCCAGCGAAATGATATCGa                                                    >  1:253208/1‑149 (MQ=255)
                                                                                             ttGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCGGAACATGCGAGTTATGGCATTCCAGCGAAATGATATCGAt                                                   >  2:312721/1‑149 (MQ=255)
                                                                                                   aGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCGGAACATGCGAGTTATGTCATTCCAGCGAAATGATATCGATGCTGGa                                             <  8:215198/149‑1 (MQ=255)
                                                                                                   aGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCGGAACATGCGAGTTATGGCATTCCAGCGAAATGATATCGATGCTGGa                                             <  5:72681/149‑1 (MQ=255)
                                                                                                         gcggcgACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCGGAACATGCGAGTTATGGCATTCCAGCGAAATGATATCGATGCTGGActgctg                                       <  1:312721/149‑1 (MQ=255)
                                                                                                                ccTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCGGAACATGCGAGTTATGGCATTCCAGCGAAATGATATCGATGCTGGACTGCTGCAGTTTg                                <  2:325259/149‑1 (MQ=255)
                                                                                                                  tGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCGGAACATGCGAGTTATGGCATTCCAGCGAAATGATATCGATGCTGGACTGCTGCAGTTTggg                              >  6:318104/1‑149 (MQ=255)
                                                                                                                      cGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCGGAACATGCGAGTTATGGCATTCCAGCGAAATGATATCGATGCTGGACTGCTGCAGTTTGGGGaa                           <  5:318104/148‑1 (MQ=255)
                                                                                                                        ggTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCGGAACATGCGAGTTATGGCATTCCAGCGAAATGATATCGATGCTGGACTGCTGCAGTTTGGGGAAGGc                        <  3:142501/149‑1 (MQ=255)
                                                                                                                        ggTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCGGAACATGCGAGTTATGGCATTCCAGCGAAATGATATCGATGCTGGACTGCTGCAGTTTGGGGAAGGc                        <  6:126510/149‑1 (MQ=255)
                                                                                                                          tgtCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCGGAACATGCGAGTTATGGCATTCCAGCGAAATGATATCGATGCTGGACTGCTGCAGTTTGGGGAAGGCCt                      <  2:253208/149‑1 (MQ=255)
                                                                                                                           gtCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCGGAACATGCGAGTTATGGCATTCCAGCGAAATGATATCGATGCTGGACTGCTGCAGTTTGGGGAAGGCCTg                     >  1:164633/1‑149 (MQ=255)
                                                                                                                            tctcAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCGGaa                                                                                        <  2:180162/81‑1 (MQ=255)
                                                                                                                            tctcAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCGGaa                                                                                        >  1:180162/1‑81 (MQ=255)
                                                                                                                              tcAATGACATGGGTGGCCACATCGGTGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCGGAACATGCGAGTTATGGCATTCCAGCGAAATGATATCGATGCTGGACTGCTGCAGTTTGGGGAAGGCCTGtt                   >  8:56317/1‑148 (MQ=255)
                                                                                                                              tcAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCGGAACATGCGAGTTATGGCATTCCAGCGAAATGATATCGATGCTGGACTGCTGCAGTTTGGGGAAGGCCTGtt                   >  8:92925/1‑148 (MQ=255)
                                                                                                                                aaTGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCGGAACATGCGAGTTATGGCATTCCAGCGAAATGATATCGATGCTGGACTGCTGCAGTTTGGGGAAGGCCTGTTc                  <  6:273411/147‑1 (MQ=255)
                                                                                                                                aaTGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCGGAACATGCGAGTTATGGCATTCCAGCGAAATGATATCGATGCTGGACTGCTGCAGTTTGGGGAAGGCCTGTTCa                 >  1:140869/1‑148 (MQ=255)
                                                                                                                                        gggTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCGGAACATGCGAGTTATGGCATTCCAGCGAAATGATATCGATGGTGGACTGCTGCAGTTTGGGGAAGGCCTGTTCATATTgccgc        >  6:59006/1‑149 (MQ=255)
                                                                                                                                           tGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAgg                                                                                                  <  6:313090/56‑1 (MQ=255)
                                                                                                                                           tGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAgg                                                                                                  >  5:313090/1‑56 (MQ=255)
                                                                                                                                              ccACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCGGAACATGCGAGTTATGGCATTCCAGCGAAATGATATCGATGCTGGACTGCTGCAGTTTGGGGAGGGCCTGTTCATATTGCCGCCATTCt  >  7:264563/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
TTGAGCTTGCCGCTGGCTACCTGGCGGGGCAGGGGCGCCATGCCGCAGTTGGTGCAAGGGTAGAGCTTGTCGGCATCGACGAATTGCAGTGCCTTGCGCAGGGTGGCGGCGACCTGTTCGGGTGTCTCAATGACATGGGTGGCCACATCGATGGCGCCGACCATGACTTTCTTGCCGCGAATCAGCTCGAGCAGGTCCATCGGAACATGCGAGTTATGGCATTCCAGCGAAATGATATCGATGCTGGACTGCTGCAGTTTGGGGAAGGCCTGTTCATATTGCCGCCATTCT  >  NC_002947/3089233‑3089523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑