Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,130,516 G→A 6.9% R111R (CGG→CGA)  PP_2747 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,130,5160GA 6.9% 170.4 / 2.3 58R111R (CGG→CGA) PP_2747hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (25/29);  new base A (2/2);  total (27/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.13e-01

CGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCATTGAGCGGTGCCTTCGAGCCGAGCCTGCTGATGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGTTGGGTAGCGGCCTTGCCGAGCTGTGGCCACTGCGTTTGTTTTCCGCACAAGCCAATGCCGTGAGCAACGGCTTGTGGCAGGTG  >  NC_002947/3130370‑3130663
                                                                                                                                                  |                                                                                                                                                   
cgcCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCATTGAGCGGTGCCTTCGAGCCGAGCCTGCTGATGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGc                                                                                                                                                    <  4:69845/148‑1 (MQ=255)
  ccAGCAGGGTTTCACCCCTGGCGCGCGACTGGCATTGAGCGGTGCCTTCGAGCCGAGCCTGCTGATGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAAc                                                                                                                                                 <  7:164116/149‑1 (MQ=255)
     gcagGGTTTCACCCCTGGCGCGCGACTGGCATTGAGCGGTGCCTTCGAGCCGAGCCTGCTGATGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTgc                                                                                                                                              >  6:55014/1‑149 (MQ=255)
            ttCACCCCTGGCGCGCGACTGGCATTGAGCGGTGCCTTCGAGCCGAGCCTGCTGATGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAgg                                                                                                                                        >  5:71978/1‑148 (MQ=255)
               aCCCCTGGCGCGCGACTGGCATTGAGCGGTGCCTTCGAGCCGAGCCTGCTGATGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGcagt                                                                                                                                    >  7:70335/1‑147 (MQ=255)
                 cccTGGCGCGCGACTGGCATTGAGCGGTGCCTTCGAGCCGAGCCTGCTGATGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATc                                                                                                                                  >  1:331668/1‑149 (MQ=255)
                      gcgcgcGACTGGCATTGAGCGGTGCCTTCGAGCCGAGCCTGCTGATGCTGGCGTTTGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCgggg                                                                                                                             <  5:234235/149‑1 (MQ=255)
                          gcgACTGGCATTGAGCGGTGCCTTCGAGCCGAGCCTGCTGATGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCg                                                                                                                         <  6:90368/149‑1 (MQ=255)
                            gACTGGCATTGAGCGGTGCCTTCGAGCCGAGCCTGCTGATGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGcc                                                                                                                       >  3:295297/1‑149 (MQ=255)
                             aCTGGCATTGAGCGGTGCCTTCGAGCCGAGCCTGCTGATGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCt                                                                                                                      <  3:161531/149‑1 (MQ=255)
                              cTGGCATTGAGCGGTGCCTTCGAGCCGAGCCTGCTGATGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCtt                                                                                                                     >  8:153942/1‑149 (MQ=255)
                               tGGCATTGAGCGGTGCCTTCGAGCCGAGCCTGCTGATGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTc                                                                                                                    >  1:347288/1‑149 (MQ=255)
                                 gCATTGAGCGGTGCCTTCGAGCCGAGCCTGCTGATGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTc                                                                                                                    <  1:337190/147‑1 (MQ=255)
                                 gCATTGAGCGGTGCCTTCGAGCCGAGCCTGCTGATGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGt                                                                                                                  >  2:86460/1‑149 (MQ=255)
                                              ccTTCGAGCCGAGCCTGCTGATGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTg                                                                                                       >  3:85744/1‑147 (MQ=255)
                                                ttCGAGCCGAGCCTGCTGATGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGa                                                                                                   >  6:301534/1‑149 (MQ=255)
                                                ttCGAGCCGAGCCTGCTGATGCTGGCGTTCGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCTCCTTCGTTCAGCGCCGTGAAGa                                                                                                   >  2:12931/1‑149 (MQ=255)
                                                   gagccgagccTGCTGATGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACg                                                                                                 >  4:78765/1‑148 (MQ=255)
                                                    agccgagccTGCTGATGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTa                                                                                               <  1:198089/149‑1 (MQ=255)
                                                       cgagccTGCTGATGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCg                                                                                            <  6:1027/149‑1 (MQ=255)
                                                          gccTGCTGATGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCAc                                                                                         >  2:47018/1‑149 (MQ=255)
                                                              gctgATGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGAc                                                                                                  <  6:155779/136‑1 (MQ=255)
                                                              gctgATGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGAc                                                                                                  >  5:155779/1‑136 (MQ=255)
                                                               ctgATGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGtt                                                                                    <  7:153942/149‑1 (MQ=255)
                                                                  aTGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGTTgg                                                                                  <  1:47018/148‑1 (MQ=255)
                                                                  aTGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGTTg                                                                                   <  5:55014/147‑1 (MQ=255)
                                                                      tGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTc                                                                                                                          >  3:267311/1‑104 (MQ=255)
                                                                      tGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGTTGGGTAGc                                                                             >  1:318077/1‑149 (MQ=255)
                                                                           ttGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGTTGGGTAGCGGCCt                                                                        >  5:100049/1‑149 (MQ=255)
                                                                                    caccaGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGTTGGGTAGCGGCCTTGCCGAGCt                                                               >  3:246577/1‑149 (MQ=255)
                                                                                    caccaGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGACAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGTTGGGTAGCGGCCTTGCCGAGCt                                                               >  8:310796/1‑149 (MQ=255)
                                                                                    caccaGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGACAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGTTGGGTAGCGGCCTTGCCGAGCt                                                               >  8:315736/1‑149 (MQ=255)
                                                                                    caccaGCTTGGCGGCCAGAGCCTGGTGATTGACCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGTTGGGTAGCGGCCTTGCCGAGCt                                                               <  5:70714/149‑1 (MQ=255)
                                                                                      ccaGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGTTGGGTAGCGGCCTTGCCGAGCtgt                                                             <  2:331668/149‑1 (MQ=255)
                                                                                       caGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGTTGGGTAGCGGCCTTGCCGAGCtgtg                                                            <  4:295297/149‑1 (MQ=255)
                                                                                       caGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGTTGGGTAGCGGCCTTGCCGAGCtgtg                                                            <  2:347288/149‑1 (MQ=255)
                                                                                           ttGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGTTGGGTAGCGGCCTTGCCGAGCTGTGCCCa                                                        >  3:7761/1‑149 (MQ=255)
                                                                                             ggcggcCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTACCGCACTGGTTGGGTAGCGGCCTTGCCGAGCTGTGGCCAc                                                       <  6:71978/148‑1 (MQ=255)
                                                                                                 gcCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGACAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGTTGGGTAGCGGCCTTGCCGAGCTGTGGCCACTGCgt                                                  <  7:315736/149‑1 (MQ=255)
                                                                                                 gcCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGACAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGTTGGGTAGCGGCCTTGCCGAGCTGTGGCCACTGCgt                                                  <  7:310796/149‑1 (MQ=255)
                                                                                                   cAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGTTGGGTAGCGGCCTTGCCGAGCTGTGGCCACTGCgttt                                                <  5:159991/149‑1 (MQ=255)
                                                                                                        ccTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGTTGGGTAGCGGCCTTGCCGAGCTGTGGCCACTGCGTTTGtttt                                           <  1:86460/149‑1 (MQ=255)
                                                                                                          tGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGTTGGGTAGCGGCCTTGCCGAGCTGTGGCCCCTGCGTTTGTTTTcc                                         >  2:33018/1‑149 (MQ=255)
                                                                                                            gTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGTTGGGTAGCGGCCTTGCCGAGCTGTGGCCACTGCGTTTGTTTTCCg                                        <  3:78765/148‑1 (MQ=255)
                                                                                                                ttGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGTTGGGTAGCGGCCTTGCCGAGCTGTGGCCACTGCGTTTGTTTTCCGCACaa                                   <  3:32852/149‑1 (MQ=255)
                                                                                                                  ggCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGTTGGGTAGCGGCCTTGCCGAGCTGTGGCCACTGCGTTTGTTTTCCGCACAAg                                  <  4:104667/148‑1 (MQ=255)
                                                                                                                   gCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCTCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGTTGGGTAGCGGCCTTGCCGAGCTGTGGGCACTGCGTTTGTTTTCCGCACAAGc                                 <  1:12931/148‑1 (MQ=255)
                                                                                                                   gCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGTTGGGTAGCGGCCTTGCCGAGCTGTGGCCACTGCGTTTGTTTTcc                                         <  7:174252/140‑1 (MQ=255)
                                                                                                                   gCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGTTGGGTAGCGGCCTTGCCGAGCTGTGGCCACTGCGTTTGTTTTcc                                         >  8:174252/1‑140 (MQ=255)
                                                                                                                          cAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGTTGGGTAGCGGCATTGCCGAGCTGTGGCCACTGCGTTTGTTTTCCGCACAAGCCAATGcct                         >  3:248546/1‑148 (MQ=255)
                                                                                                                           aGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGTTGGGTAGCGGCCTTGCCGAGCTGTGGCCACTGCGTTTGTTTTCCGCACAAGCCAATGCCGt                        <  2:318077/149‑1 (MQ=255)
                                                                                                                             ccGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGTTGGGTAGCGGCCTTGCCGAGCTGTGGCCACTGCGTTTGTTTTCCGCACaa                                   >  3:105809/1‑136 (MQ=255)
                                                                                                                             ccGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGTTGGGTAGCGGCCTTGCCGAGCTGTGGCCACTGCGTTTGTTTTCCGCACaa                                   <  4:105809/136‑1 (MQ=255)
                                                                                                                             ccGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGTTGGGTAGCGGCCTTGCCGAGCTGTGGCCACTGCGTTTGTTTTCCGCACAAGCCAATGCCGTGa                      <  4:85744/149‑1 (MQ=255)
                                                                                                                                   cGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGTTGGGTAGCGGCCTTGCCGAGCTGTGGCCACTGCGTTTGTTTTCCGCACAAGCCAATGCCGTGAGCAACg                >  3:73960/1‑149 (MQ=255)
                                                                                                                                          cTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGGGCGTTGAAGACGTATCGCACTGGTTGGGTAGCGGCGTTGCCGTGCTGGGGGCACTGCGTTTGTTTTCCGNACAAGCCAATGCCGTGAGCAACGGCTtgtg         <  1:33018/149‑1 (MQ=255)
                                                                                                                                          cTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGTTGGGTAGCGGCCTTGCCGAGCTGTGGCCACTGCGTTTGTTTTCCGCACAAGCCAATGCCGTGAGCAACGGCtt            >  6:8784/1‑146 (MQ=255)
                                                                                                                                          cTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGTTGGGTAGCGGCCTTGCCGAGCTGTGGCCACTGCGTTTGTTTTCCGCACAAGCCAATGCCGTGAGCAACGGCtt            <  5:8784/146‑1 (MQ=255)
                                                                                                                                                 ggCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGTTGGGTAGCGGCCTTGCCGAGCTGTGGCCACTGCGTTTGTTTTCCGCACAAGCCAATGCCGTGAGCAACGGCTTGTGGc       <  3:62644/144‑1 (MQ=255)
                                                                                                                                                 ggCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGTTGGGTAGCGGCCTTGCCGAGCTGTGGCCACTGCGTTTGTTTTCCGCACAAGCCAATGCCGTGAGCAACGGCTTGTGGc       >  4:62644/1‑144 (MQ=255)
                                                                                                                                                  gCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGTTGGGTAGCGGCCTTGCCGAGCTGTGGCCACTGCGTTTGTTTTCCGCACAAGCCAATGCCGTGAGCAACGGCTTGTGGCAGGTg  >  2:312332/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CGCCAGCAGGGTTTCACCCCTGGCGCGCGACTGGCATTGAGCGGTGCCTTCGAGCCGAGCCTGCTGATGCTGGCGTTGGCGGCCCACCAGCTTGGCGGCCAGAGCCTGGTGATTGGCCGCGACAGCCGTGGCGATGACCTGCAACGGCAACTGCGCCAGGCACATCCGGGGTTCGCCTTCGTTCAGCGCCGTGAAGACGTATCGCACTGGTTGGGTAGCGGCCTTGCCGAGCTGTGGCCACTGCGTTTGTTTTCCGCACAAGCCAATGCCGTGAGCAACGGCTTGTGGCAGGTG  >  NC_002947/3130370‑3130663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑