Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 280,203 G→T 10.5% L16M (CTG→ATG) ‡ sctS ← sulfur compound ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947280,2030GT10.5% 245.2 / 15.0 85L16M (CTG→ATG) ‡sctSsulfur compound ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (33/43);  new base T (4/5);  total (37/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.52e-01

GCGGAAGAACGACACGTAGACCCGCGCCAGCCAGTTCAGCGGCAGAATCTTCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTTCTCGGACAGCTTGGCCAGCGT‑GCCATCAGCTTTGAGCTTGTCGATGGCCTTGTTGATGGCTGCCAACAGTTCCGG  >  NC_002947/280057‑280349
                                                                                                                                                  |                                                                                                                                                   
gcgGAAGAACGACACGTAGACCCGCGCCAGCCAGTTCAGCGGCAGAATCTTCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGAGTAACCCGCGCCCTTCAGCAGGa                                                                                                                                                   <  7:229135/149‑1 (MQ=255)
      gaaCGACACGTAGACCCGCGCCAGCCAGTTCAGCGGCAGAATCTTCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGgcg                                                                                                                                             <  2:87492/149‑1 (MQ=255)
       aaCGACACGTAGACCCGCGCCAGCCAGTTCAGCGGCAGAATCTTCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGgcgc                                                                                                                                            >  2:255269/1‑149 (MQ=255)
         cGACACGTAGACCCGCGCCAGCCAGTTCAGCGGCAGAATCTTCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCCTGCTGAAGGGCGCgg                                                                                                                                          <  5:163260/149‑1 (MQ=255)
         cGACACGTAGACCCGCGCCAGCCAGTTCAGCGGCAGAATCTTCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCCTGCTGAAGGGCGCgg                                                                                                                                          <  2:115405/149‑1 (MQ=255)
          gACACGTAGACCCGCGCCAGCCAGTTCAGCGGCAGAATCTTCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGcgcg                                                                                                                                           >  6:117909/1‑147 (MQ=255)
          gACACGTAGACCCGCGCCAGCCAGTTCAGCGGCAGAATCTTCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGa                                                                                                                                         <  6:234346/149‑1 (MQ=255)
             acGTAGCCCCGCGCCAGCCAGTTCAGCGGCAGAATCTTCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCCGCAGGAAGGGCGCGGAGTc                                                                                                                                      >  4:205773/1‑149 (MQ=255)
               gTAGACCCGCGCCAGCCAGTTCAGCGGCAGAATCTTCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGa                                                                                                                                    >  5:103571/1‑149 (MQ=255)
               gTAGACCCGCGCCAGCCAGTTCAGCGGCAGAATCTTCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGa                                                                                                                                    <  5:117909/149‑1 (MQ=255)
                     ccGCGCCAGCCAGTTCAGCGGCAGAATCTTCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCCTGCTGAAGGGcgcg                                                                                                                                           <  1:279755/136‑1 (MQ=255)
                     ccGCGCCAGCCAGTTCAGCGGCAGAATCTTCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCCTGCTGAAGGGcgcg                                                                                                                                           >  2:279755/1‑136 (MQ=255)
                     ccGCGCCAGCCAGTTCAGCGGCAGAATCTTCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGa                                                                                                                              >  8:323113/1‑149 (MQ=255)
                     ccGCCCCAGCCAGTGCCGCGGCATAATCTTCGACACCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACCTGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGa                                                                                                                              <  8:318557/149‑1 (MQ=255)
                      cgcgCCAGCCAGTTCAGCGGCAGAATCTTCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCCTGCTGAAGGGCGCgg                                                                                                                                          <  4:258834/136‑1 (MQ=255)
                      cgcgCCAGCCAGTTCAGCGGCAGAATCTTCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCCTGCTGAAGGGCGCgg                                                                                                                                          >  3:258834/1‑136 (MQ=255)
                      cgcgCCAGCCAGTTCAGCGGCAGAATCTTCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAg                                                                                                                             >  4:139743/1‑149 (MQ=255)
                       gcgcCAGCCAGTTCAGCGGCAGAATCTTCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGt                                                                                                                            >  6:170193/1‑149 (MQ=255)
                          ccagccagTTCAGCGGCAGAATCTTCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGc                                                                                                                         <  1:319370/149‑1 (MQ=255)
                           cagccagTTCAGCGGCAGAATCTTCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCATCACCTTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCa                                                                                                                        <  6:300871/149‑1 (MQ=255)
                           cagccagTTCAGCGGCAGAATCTTCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCa                                                                                                                        <  4:131121/149‑1 (MQ=255)
                            agccagTTCAGCGGCAGAATCTTCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGCAGGGCGCGGAGTCGACAACGAGTTGCAg                                                                                                                       >  5:158135/1‑149 (MQ=255)
                              ccagTTCAGCGGCAGAATCTTCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGc                                                                                                                     >  7:122648/1‑149 (MQ=255)
                                    cAGCGGCAGAATCTTCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCa                                                                                                                <  3:238320/148‑1 (MQ=255)
                                     aGCGGCAGAATCTTCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGc                                                                                                              >  6:97296/1‑149 (MQ=255)
                                      gCGGCAGAATCTTCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCg                                                                                                             <  8:77357/149‑1 (MQ=255)
                                        ggCAGAATCTTCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAg                                                                                                               >  1:358292/1‑145 (MQ=255)
                                        ggCAGAATCTTCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAg                                                                                                               <  2:358292/145‑1 (MQ=255)
                                         gCAGAATCTTCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCCTGCTGAAGGGCGCgg                                                                                                                                          <  6:215663/117‑1 (MQ=255)
                                         gCAGAATCTTCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCCTGCTGAAGGGCGCgg                                                                                                                                          >  5:215663/1‑117 (MQ=255)
                                          cAGAATCTTCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGaa                                                                                                                                                  <  7:133186/108‑1 (MQ=255)
                                          cAGAATCTTCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGaa                                                                                                                                                  >  8:133186/1‑108 (MQ=255)
                                          cAGAATCTTCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTc                                                                                                                                      >  7:22352/1‑120 (MQ=255)
                                          cAGAATCTTCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTc                                                                                                                                      <  8:22352/120‑1 (MQ=255)
                                          cAGAATCTTCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCa                                                                                                         <  3:139743/149‑1 (MQ=255)
                                                 ttCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGt                                                                                                   >  6:264575/1‑148 (MQ=255)
                                                   cGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTgg                                                                                                    <  1:46333/145‑1 (MQ=255)
                                                        gCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAATGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCgg                                                                                           >  4:170443/1‑149 (MQ=255)
                                                         ccGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTc                                                                                                                 >  5:25359/1‑126 (MQ=255)
                                                         ccGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTc                                                                                                                 <  6:25359/126‑1 (MQ=255)
                                                                 gcgcCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAg                                                                                   >  6:152838/1‑148 (MQ=255)
                                                                  cgcCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTa                                                                                 <  6:103571/149‑1 (MQ=255)
                                                                   gcCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTAc                                                                                <  6:157654/149‑1 (MQ=255)
                                                                     cAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACaa                                                                                                                                 <  6:339319/98‑1 (MQ=255)
                                                                     cAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACaa                                                                                                                                 >  5:339319/1‑98 (MQ=255)
                                                                      aGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACtt                                                                              >  2:50984/1‑148 (MQ=255)
                                                                      aGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACtt                                                                              <  1:50984/148‑1 (MQ=255)
                                                                       gggCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTtct                                                                            <  5:170193/149‑1 (MQ=255)
                                                                        ggCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTtctc                                                                           <  3:345536/149‑1 (MQ=255)
                                                                             aGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTTCTCGGa                                                                        >  2:346237/1‑147 (MQ=255)
                                                                                  aGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGGGATCATTTGGTGACATCGGCACCGAAGTACTTCTCGGACAGCTTg                                                                 <  1:255269/149‑1 (MQ=255)
                                                                                    cacaAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCATGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTAAGCGATCAtttc                                                                                                     >  4:89139/1‑110 (MQ=255)
                                                                                    cACCAGACCGAAGAACATGCGGGCTACACTGATCAGCACCGTATAACCCGCGGCCTTCAGCAGGAATGGCGCGGAGTCGACAACGTGTTGCAGGCTTTCAGCGATCATTTg                                                                                                     <  3:89139/111‑1 (MQ=255)
                                                                                      ccAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTTCTCGGACAGCTTGGCCa                                                             <  5:97296/149‑1 (MQ=255)
                                                                                           ccGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTTCTCGGACAGCTTgg                                                                <  5:308102/141‑1 (MQ=255)
                                                                                           ccGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTTCTCGGACAGCTTgg                                                                >  6:308102/1‑141 (MQ=255)
                                                                                           ccGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTTCTCGGACAGCTTGGCCAGCGT‑g                                                       <  5:264575/149‑1 (MQ=255)
                                                                                           ccGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTTCTCGGACAGCTTGGCCAGCGT‑g                                                       <  7:323113/149‑1 (MQ=255)
                                                                                            cGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTTCTCGGACAGCTTGGCCAGCGT‑Gc                                                      >  4:207748/1‑149 (MQ=255)
                                                                                              aagaaCATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGc                                                                                                                         <  3:7601/81‑1 (MQ=255)
                                                                                              aagaaCATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGc                                                                                                                         >  4:7601/1‑81 (MQ=255)
                                                                                                       ccgccgACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAATGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTTCTCGGACAGCTTGGCCAGCGT‑GCCATCAGCttt                                            <  3:170443/148‑1 (MQ=255)
                                                                                                        cgccgACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTTCTCGGACAGCTTGGCCAGCGT‑GCCATCAGCTTTGa                                          <  3:207748/149‑1 (MQ=255)
                                                                                                         gccgACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTTCTCGGACAGCTTGGCCAGCGT‑GCCATCa                                                 <  3:311383/141‑1 (MQ=255)
                                                                                                         gccgACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTTCTCGGACAGCTTGGCCAGCGT‑GCCATCa                                                 >  4:311383/1‑141 (MQ=255)
                                                                                                         gccgACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTTCTCGGACAGCTTGGCCAGCGT‑GCCATCAGCTTTGAg                                         <  2:346078/149‑1 (MQ=255)
                                                                                                               acTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTTCTCGGACATCTTGGCCAGCGT‑GCCATCAGCTTTGAGCTTGTc                                   >  4:225300/1‑149 (MQ=255)
                                                                                                               acTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTTCTCGGACAGCTTGGCCAGCGT‑GCCATCAGCTTTGa                                          >  6:220462/1‑142 (MQ=255)
                                                                                                               acTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTTCTCGGACAGCTTGGCCAGCGT‑GCCATCAGCTTTGa                                          <  5:220462/142‑1 (MQ=255)
                                                                                                                    gcagcaCCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTTCTCGGACAGCTTg                                                                 <  6:211221/115‑1 (MQ=255)
                                                                                                                     cagcaCCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGTCAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTTCTCGGACAGCTTGGCCAGCGT‑GCCATCAGCTTTGAGCTTGTCGATGGc                             <  3:205773/149‑1 (MQ=255)
                                                                                                                     cagcaCCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTTCTCGGACAGCTTGGCCAGCGT‑GCCATCAGCTTTGAGCTTGTCGATGGc                             >  6:293406/1‑149 (MQ=255)
                                                                                                                       gcaCCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTTCTCGGACAGCTTGGCCAGCGT‑GCCATCAGCTTTGAGc                                        <  4:70082/136‑1 (MQ=255)
                                                                                                                       gcaACGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTTCTCGGACAGCTTGGCCAGCGT‑GCCATCAGCTTTGAGc                                        >  3:70082/1‑136 (MQ=255)
                                                                                                                        caCCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTTCTCGGACAGCTTGGCCAGCGT‑GCCATCAGCTTTGAGCTTGTCGATGGCCtt                          >  8:31524/1‑149 (MQ=255)
                                                                                                                          ccGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTTCTCGGACAGCTTGGCCAGCGT‑GCCATCAGCTTTGAGCTTGTCGATGGCCttgt                        <  5:293406/149‑1 (MQ=255)
                                                                                                                               taACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGTCACCGAAGTACTTCTCGGACAGCTTGGCCAGCGT‑GCCATCAGCTTTGAGCTTGTCGATGGCCTTGTTGAt                    <  7:31524/148‑1 (MQ=255)
                                                                                                                               taACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTTCTCGGACAGCTTGGCCAGCGT‑GCCATCAGCTTTGAGCTTGTCGATGGCCTTGTTGATg                   >  3:289089/1‑149 (MQ=255)
                                                                                                                                   ccGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTTCTCGGACAGCTTGGCCAGCGT‑GCCATCAGCTTTGAGCTTGTCGATGGCCTTGTTGATGGCTg               >  1:100004/1‑149 (MQ=255)
                                                                                                                                   ccGCGCCCTACAGCAGGACGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTTCTCGGACAGCTTGGCCAGCGT‑GCCATCAGCTTTGAGCTTGTCGATGGCCTTGTTGATGGCTg               <  8:122648/149‑1 (MQ=255)
                                                                                                                                       gcCCTTCAGCAGGCAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTTCTCGGACAGCTTGGCCAGCGT‑GCCATCAGCTTTGAGCTTGTCGATGGCCTTGTTGATGGCTGCCaa           <  6:158135/149‑1 (MQ=255)
                                                                                                                                       gcCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTTCTCGGACAGCTTGGCCAGCGT‑GCCATCAGCTTTGAGCTTGTCGATGGCCtt                          >  1:56360/1‑134 (MQ=255)
                                                                                                                                       gcCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTTCTCGGACAGCTTGGCCAGCGT‑GCCATCAGCTTTGAGCTTGTCGATGGCCtt                          <  2:56360/134‑1 (MQ=255)
                                                                                                                                       gcCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTTCTCGGACAGCTTGGCCAGCGT‑GCCATCAGCTTTGAGCTTGTCGATGGCCTTGTTGATGGCTGCCaa           <  1:346237/149‑1 (MQ=255)
                                                                                                                                           ttCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTTCTCGGACAGCTTGGCCAGCGT‑GCCATCAGCTTTGAGCTTGTCGATGGCCTTGTTGATGGCTGCCAACAGt       >  8:172227/1‑149 (MQ=255)
                                                                                                                                              agcagGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTTCTCGGACAGCTTGGCCAGCGTAGCCATCAGCTTTGAGCTTGTCGATGGCCTTGTTGATGGCTGCCAACAGTTc     <  1:271191/149‑1 (MQ=255)
                                                                                                                                              agcagGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTTCTCGGACAGCTTGGCCAGCGT‑GCCATCAGCTTTGAGCTTGTCGATGGCCTTGTTGATGGCTGCCAACAGTTcc    <  2:100004/149‑1 (MQ=255)
                                                                                                                                               gcagGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCATTCAGCGATCATTTGGAGACATCGGCACCGAAGTACTTCTCGGACAGCTTGGACAGCGT‑GAGATCAGCTTTGAGCTTCTCGAAGGCCTTGTTGATGGCTGCCAAAAGTTc     >  1:272737/1‑147 (MQ=255)
                                                                                                                                                caggcaggGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTTCTCGGACAGCTTGGCCAGCGT‑GCCATCAGCTTTGAGCTTGTCGATGGCCTTGTTGATGGCTGCCAACAGTTCCgg  <  5:199605/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GCGGAAGAACGACACGTAGACCCGCGCCAGCCAGTTCAGCGGCAGAATCTTCGACAGCCGCATCAGCGCCAGGGCAAAGCCCAGCACCAGACCGAAGAACATGCCGCCGACACTGAGCAGCACCGTATAACCCGCGCCCTTCAGCAGGAAGGGCGCGGAGTCGACAACGAGTTGCAGGCTTTCAGCGATCATTTGGTGACATCGGCACCGAAGTACTTCTCGGACAGCTTGGCCAGCGT‑GCCATCAGCTTTGAGCTTGTCGATGGCCTTGTTGATGGCTGCCAACAGTTCCGG  >  NC_002947/280057‑280349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑