Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,137,653 A→G 6.3% intergenic (+81/‑209) PP_2753 → / → PP_2754 ABC transporter ATP‑binding protein/OprD family outer membrane porin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,137,6530AG 6.3% 229.6 / 2.5 78intergenic (+81/‑209)PP_2753/PP_2754ABC transporter ATP‑binding protein/OprD family outer membrane porin
Reads supporting (aligned to +/- strand):  ref base A (38/35);  new base G (3/2);  total (41/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGCAGTGCTGCCGAGCTGGCCGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGTTTTTTTTTGTTGGCATGGTTGTTG  >  NC_002947/3137506‑3137800
                                                                                                                                                   |                                                                                                                                                   
gggCAGTGCTGCCGAGCTGGCCGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAg                                                                                                                                                    >  6:13370/1‑149 (MQ=255)
 ggCAGTGCTGCCGAGCTGGCCGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAgg                                                                                                                                                   >  4:163846/1‑149 (MQ=255)
        ctgcCGAGCTGGCCGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCTGGGCCGCGtt                                                                                                                                            <  5:6915/149‑1 (MQ=255)
        ctgcCGAGCTGGCCGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGtt                                                                                                                                            >  3:130573/1‑149 (MQ=255)
         tgcCGAGCTGGCCGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGttt                                                                                                                                           >  1:304682/1‑149 (MQ=255)
           ccGAGCTGGCCGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGttttt                                                                                                                                         <  2:217334/149‑1 (MQ=255)
           ccGAGCTGGCCGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGttt                                                                                                                                           <  7:142655/147‑1 (MQ=255)
             gAGCTGGCCGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGATGTGGAGAGGAGCAGTCATAACGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGttttttt                                                                                                                                       >  6:302587/1‑149 (MQ=255)
                   gCCGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTAGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCt                                                                                                                                 <  3:69606/149‑1 (MQ=255)
                            gAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAgc                                                                                                                        >  1:6068/1‑149 (MQ=255)
                             aaGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAgcg                                                                                                                       <  2:172765/149‑1 (MQ=255)
                              aGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAgcgc                                                                                                                      >  5:285242/1‑149 (MQ=255)
                                 tatTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGCGCGGGGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCtgt                                                                                                                   <  6:243536/149‑1 (MQ=255)
                                 tatTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCtgt                                                                                                                   >  4:8146/1‑149 (MQ=255)
                                 tatTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCtgt                                                                                                                   <  5:339438/149‑1 (MQ=255)
                                   ttCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTgg                                                                                                                 >  4:332553/1‑149 (MQ=255)
                                    tCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGgc                                                                                                                >  5:192989/1‑149 (MQ=255)
                                    tCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGgc                                                                                                                >  3:219587/1‑149 (MQ=255)
                                     cAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCt                                                                                                                                 <  2:10545/131‑1 (MQ=255)
                                      aGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGgcgc                                                                                                              >  7:145331/1‑149 (MQ=255)
                                      aGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGgcgc                                                                                                              >  2:431/1‑149 (MQ=255)
                                       gCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGTTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGcc                                                                                                             <  6:183633/149‑1 (MQ=255)
                                         aTTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCgg                                                                                                           >  3:329976/1‑149 (MQ=255)
                                         aTTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCgg                                                                                                           <  3:332553/149‑1 (MQ=255)
                                             cTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCaa                                                                                                        <  6:192989/148‑1 (MQ=255)
                                                ccTTGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAAc                                                                                                       <  8:95962/146‑1 (MQ=255)
                                                ccTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAAca                                                                                                      <  1:431/147‑1 (MQ=255)
                                                  tGGGCGGCAAGGGCTGACTGAGCGGTGGGGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAAcagcag                                                                                                  >  1:202864/1‑149 (MQ=255)
                                                      cggcAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTtgt                                                                                              <  5:13370/149‑1 (MQ=255)
                                                        gcAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGc                                                                                            <  3:294294/149‑1 (MQ=255)
                                                               ctgactgaGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGc                                                                                     >  6:221262/1‑149 (MQ=255)
                                                                  actgaGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCtt                                                                                   <  4:130573/148‑1 (MQ=255)
                                                                          gTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTtgtg                                                                                             >  5:328196/1‑130 (MQ=255)
                                                                          gTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTtgtg                                                                                             <  6:328196/130‑1 (MQ=255)
                                                                          gTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACgg                                                                          <  2:202864/149‑1 (MQ=255)
                                                                           tGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACg                                                                           >  6:167731/1‑147 (MQ=255)
                                                                               gACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCTGGGCCGCGttga                                                                                                                                          >  5:267793/1‑78 (MQ=39)
                                                                               gACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCTGGGCCGCGttga                                                                                                                                          <  6:267793/80‑3 (MQ=39)
                                                                               gACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGcat                                                                     <  3:163846/149‑1 (MQ=255)
                                                                                   gCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATa                                                                 <  2:6068/149‑1 (MQ=255)
                                                                                   gCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATa                                                                 <  6:285242/149‑1 (MQ=255)
                                                                                    cTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGGTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGAAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAg                                                                >  8:181446/1‑149 (MQ=255)
                                                                                     tgtgGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAgt                                                               >  3:187598/1‑149 (MQ=255)
                                                                                       tgGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAgt                                                               <  2:304682/147‑1 (MQ=255)
                                                                                         gAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTTATGTTCCATTCAACGCGGCCCAGTACGCGTTTTTTTCGCCCTGACTAAAGCGCTGTGGCGCCGTTAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGtt                                                            <  5:302587/148‑1 (MQ=255)
                                                                                                gcAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCATTTGTGCAGATTGCTTCGCAGACGGAGCATCATa                                                                 <  1:155996/136‑1 (MQ=255)
                                                                                                gcAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATa                                                                 >  2:155996/1‑136 (MQ=255)
                                                                                                 cAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAgtt                                                   <  8:145331/149‑1 (MQ=255)
                                                                                                    tCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGaa                                                      <  1:136870/143‑1 (MQ=255)
                                                                                                    tCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGaa                                                      >  2:136870/1‑143 (MQ=255)
                                                                                                      aTACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTc                                               >  3:244606/1‑148 (MQ=255)
                                                                                                      aTACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTc                                               <  4:329976/148‑1 (MQ=255)
                                                                                                         ccGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAgtt                                                   >  1:353207/1‑141 (MQ=255)
                                                                                                         ccGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAgtt                                                   <  2:353207/141‑1 (MQ=255)
                                                                                                         ccGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAAt                                            <  5:167731/148‑1 (MQ=255)
                                                                                                            ccTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCt                                        >  6:266464/1‑149 (MQ=255)
                                                                                                              ttttACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAgttgtt                                                >  4:293566/1‑139 (MQ=255)
                                                                                                              ttttACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAgttgtt                                                <  3:293566/139‑1 (MQ=255)
                                                                                                              ttttACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCtt                                       >  8:264160/1‑148 (MQ=255)
                                                                                                              ttttACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCtt                                       >  7:115974/1‑148 (MQ=255)
                                                                                                                      aGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACaa                              >  7:200109/1‑149 (MQ=255)
                                                                                                                        tAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATg                            >  7:120321/1‑149 (MQ=255)
                                                                                                                          ggTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGt                          >  3:167192/1‑149 (MQ=255)
                                                                                                                           gTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGtt                         >  5:77487/1‑149 (MQ=255)
                                                                                                                                 ttCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGtttttttt                   >  6:342222/1‑149 (MQ=255)
                                                                                                                                   ccATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGtttttttttt                 >  2:19739/1‑148 (MQ=255)
                                                                                                                                    aaaaaaaCGCGGCCTGGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGTTTTTTTttgt                >  5:230514/6‑149 (MQ=255)
                                                                                                                                    aaaaaaaCCCGGCCGGGGCCGCGTTTTTTTAGCCCGGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGAATGCTTCGCAGAAGGAGCATGATATTGTTGAGGAAGTTTTTGAATTGCGTGAATAAAAAAGGTTTTTTTttgt                >  2:189341/6‑149 (MQ=255)
                                                                                                                                     aTTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGTTTTTTTttgtt               <  8:120321/149‑1 (MQ=255)
                                                                                                                                         aaCGCGGCCGAGGCCGCGTTTTTTTCTCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGGATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGTTTTTTTTTGTTGGc            <  5:221262/148‑1 (MQ=255)
                                                                                                                                         aaCGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGTTTTTTTTTGTTGGCa           <  3:8146/149‑1 (MQ=255)
                                                                                                                                          aCGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGTTTTTTTTTGTTGGCAt          >  7:68401/1‑149 (MQ=255)
                                                                                                                                          aCGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGTTTTTTTTTGTTGGCAt          >  6:136991/1‑149 (MQ=255)
                                                                                                                                              ggCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCACATTGCTTCGCAGCCGGAGCATCATAGTGTTGAGGAAGTTCTTCAATTGCTTGAATAACAATGGTTTTTTTTTGTTGGAATTgtt      >  2:116745/1‑149 (MQ=255)
                                                                                                                                                 ccAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGttttttttt                  >  7:10389/1‑134 (MQ=255)
                                                                                                                                                 ccAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGttttttttt                  <  8:10389/134‑1 (MQ=255)
                                                                                                                                                  cAGGCCGGGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGTTTTTTTTTGTTGGCATGGttgttg  <  7:181446/149‑1 (MQ=255)
                                                                                                                                                  cAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGTTTTTTTTTGTTGGCATGGttgttg  <  8:115974/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GGGCAGTGCTGCCGAGCTGGCCGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGTTTTTTTTTGTTGGCATGGTTGTTG  >  NC_002947/3137506‑3137800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑