Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,147,711 T→C 6.4% D53G (GAC→GGC)  sfnA ← acyl‑CoA dehydrogenase/oxidase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,147,7110TC 6.4% 182.3 / 2.7 65D53G (GAC→GGC) sfnAacyl‑CoA dehydrogenase/oxidase family protein
Reads supporting (aligned to +/- strand):  ref base T (31/28);  new base C (2/2);  total (34/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

TCGACGAAAGCAAAATGCCCGCGCAGTGCCTGCGGCACATTGGAGTCGGCTTCGGCCAGTTCGATCAGCAGCTGGAACAACTGTGGCAGGGAA‑GCGCCGGCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATGGGGCGGAAGCGGGCGGCAAGGGATTCGTAGTGGGTACCGGTAGACAGGTGGGGCAGTGGGGCTT  >  NC_002947/3147567‑3147858
                                                                                                                                                 |                                                                                                                                                    
tCGACGAAAGCAAAATGCCCGCGCAGTGCCTGCGGCACATTGGAGTCGGCTTCGGCCAGTTCGATCAGCAGCTGGAACAACTGTGGCAGGGAA‑GCGCCGGCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCtt                                                                                                                                                  >  2:719/1‑149 (MQ=255)
     gaAAGCAAAATGCCCGCGCAGTGCCTGCGGCACATTGGAGTCGGCTTCGGCCAGTTCGATCAGCAGCTGGAACAACTGTGGCAGGGAA‑GCGCCGGCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCATCCTTGAGc                                                                                                                                              >  2:46958/1‑148 (MQ=255)
        aGCAAAATGCCCGCGCAGTGCCTGCGGCACATTGGAGTCGGCTTCGGCCAGTTCGATCAGCAGCTGGAACAACTGTGGCAGGGAA‑GCGCCGGCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACt                                                                                                                                          <  2:75250/149‑1 (MQ=255)
             aaTGCCCGCGCAGTGCCTGCGGCACATTGGAGTCGGCTTCGGCCAGTTCGATCAGCAGCTGGAACAACTGTGGCAGGGAA‑GCGCCGGCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTgg                                                                                                                                        <  6:135074/146‑1 (MQ=255)
             aaTGCCCGCGCAGTGCCTGCGGCACATTGGAGTCGGCTTCGGCCAGTTCGATCAGCAGCTGGAACAACTGTGGCAGGGAA‑GCGCCGGCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTgg                                                                                                                                        >  5:135074/1‑146 (MQ=255)
                  ccGCGCAGTGCCTGCGGCACATTGGAGTCGGCTTCGGCCAGTTCGATCAGCAGCTGGAACAACTGTGGCAGGGAA‑GCGCCGGCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCt                                                                                                                                >  6:40142/1‑148 (MQ=255)
                          tgccgcaggCACATTGGAGTCGGCTTCGGCCAGTTCGATCAGCAGCTGGAACAACTGTGGCAGGGAA‑GCGCCGGCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCa                                                                                                                       <  8:88907/142‑1 (MQ=255)
                                   cacaTTGGAGTCGGCTTCGGCCAGTTCGATGAGCAGCTGGAACAACTGTGGCAGGGAA‑GCGCCGGCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCtt                                                                                                                                                  >  1:31061/1‑114 (MQ=255)
                                   cacaTTGGAGTCGGCTTCGGCCAGTTCGATCAGCAGCTGGAACAACTGTGGCAGGGAA‑GCGCCGGCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCtt                                                                                                                                                  <  2:31061/114‑1 (MQ=255)
                                   cacaTTGGAGTCGGCTTCGGCCAGTTCGATCAGCAGCTGGAACAACTGTGGCAGGGAA‑GCGCCGGCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGt                                                                                                              >  5:175833/1‑149 (MQ=255)
                                    acaTTGGAGTCGGCTTCGGCCAGTTCGATCAGCAGCTGGAACAACTGTGGCAGGGAATTCGCCGGCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGACCTTTAGCCACTGGAT‑GGGCGCATGGGGCAGGGTGCgga                                                                                                              >  1:102863/1‑148 (MQ=255)
                                    acaTTGGAGTCGGCTTCGGCCAGTTCGATCAGCAGCTGGAACAACTGTGGCAGGGAA‑GCGCCGGCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTc                                                                                                             <  6:10358/149‑1 (MQ=255)
                                        tGGAGTCGGCTTCGGCCAGTTCGATCAGCAGCTGGAACAACTGTGGCAGGGAA‑GCGCCGGCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCgcgc                                                                                                         >  8:192954/1‑149 (MQ=255)
                                          gAGTCGGCTTCGGCCAGTTCGATCAGCAGCTGGAACAACTGTGGCAGGGAA‑GCGCCGGCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTc                                                                                                       >  1:141060/1‑149 (MQ=255)
                                              cGGCTTCGGCCAGTTCGATCAGCAGCTGGAACAACTGTGGCAGGGAA‑GCGCCGGCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGt                                                                                                   <  5:40142/149‑1 (MQ=255)
                                               ggCTTCGGCCAGTTCGATCAGCAGCTGGAACAACTGTGGCAGGGAA‑GCGCCGGCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGt                                                                                                   <  7:192954/148‑1 (MQ=255)
                                                gCTTCGGCCAGTTCGATCAGCAGCTGGAACAACTGTGGCAGGGAA‑GCGCCGGCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGc                                                                                                 <  4:180223/149‑1 (MQ=255)
                                                     ggCCAGTTCGATCAGCAGCTGGAACAACTGTGGCAGGGAA‑GCGCCGGCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGc                                                                                            >  2:36604/1‑149 (MQ=255)
                                                     ggCCAGTTCGATCAGCAGCTGGAACAACTGTGGCAGGGAA‑GCGCCGGCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGc                                                                                            >  8:252385/1‑149 (MQ=255)
                                                          gTTCGATCAGCAGCTGGAACAACTGTGGCAGGGAA‑GCGCCGGCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGAt                                                                                       >  6:70385/1‑149 (MQ=255)
                                                                    cagcTGGAACAACTGTGGCAGGGAA‑GCGCCGGCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGc                                                                             >  2:287587/1‑149 (MQ=255)
                                                                     agcTGGAACAACTGTGGCAGGGAA‑GCGCCGGCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTg                                                                                          <  7:176938/135‑1 (MQ=255)
                                                                     agcTGGAACAACTGTGGCAGGGAA‑GCGCCGGCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTg                                                                                          >  8:176938/1‑135 (MQ=255)
                                                                          gAACAACTGTGGCAGGGAA‑GCGCCGGCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGCCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGc                                                                             >  3:70993/1‑143 (MQ=255)
                                                                          gAACAACTGTGGCAGGGAA‑GCGCCGGCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGCCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGc                                                                             >  3:76051/1‑143 (MQ=255)
                                                                          gAACAACTGTGGCAGGGAA‑GCGCCGGCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGCCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGc                                                                             <  4:76051/143‑1 (MQ=255)
                                                                          gAACAACTGTGGCAGGGAA‑GCGCCGGCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGCCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGc                                                                             <  4:70993/143‑1 (MQ=255)
                                                                                  ctctcAGGGAA‑GCGCCGGCACGGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTGCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATgggg                                                               <  7:58408/145‑1 (MQ=255)
                                                                                   tgGCAGGGAA‑GCGCCGGCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATggg                                                                <  1:719/147‑1 (MQ=255)
                                                                                    ggCAGGGAA‑GCGCCGGCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATGGGGCg                                                             >  6:275975/1‑149 (MQ=255)
                                                                                      cAGGGAA‑GCGCCGGCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATGGGGCgg                                                            >  2:85204/1‑148 (MQ=255)
                                                                                       aGGGAA‑GCGCCGGCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATgagg                                                               >  8:58408/1‑144 (MQ=255)
                                                                                       aGGGAA‑GCGCCGGCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATGGGGCgg                                                            >  7:287729/1‑147 (MQ=255)
                                                                                          gAA‑GCGCCGGCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATGGGGCGGAAgcg                                                       >  2:168493/1‑149 (MQ=255)
                                                                                                   ggCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATGGGGCGGAAGCGGGCGGCaa                                               <  1:85204/149‑1 (MQ=255)
                                                                                                    gCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATGGGGCGGAAGCGGGCGGCAAg                                              >  8:140565/1‑149 (MQ=255)
                                                                                                    gCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATGGGGCGGAAGCGGGCGGCAAg                                              <  1:36604/149‑1 (MQ=255)
                                                                                                    gCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATGGGGCGGAAGCGGGCGGCAAg                                              <  7:252385/149‑1 (MQ=255)
                                                                                                      aCCGACCTGTTCGACGGGTACACGCACGGCTCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GTGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCGACGCTGGATTCGGCGATGCGGGTAAAGATGGTGCGGAAgaggtcggaatgg                                             >  8:234109/1‑143 (MQ=255)
                                                                                                       ccGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATGGGGCGGAAGCGGGCGGCAAGGGa                                           <  8:287729/149‑1 (MQ=255)
                                                                                                        cGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATGGGGCGGAAGCGGGCGGCAAGGGAt                                          <  6:77671/149‑1 (MQ=255)
                                                                                                        cGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATGGGGCGGAAGCGGGCGGCAAGGGAt                                          >  6:218625/1‑149 (MQ=255)
                                                                                                          cccTGTTCGACGGGTACACGCACGGCGGCGAAGGCGGCGTCCTTGAGCCAGTGGAT‑GGGCTCATGGGGGAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATGGGGCGGAAGCGGGCGGCAAGGGATTc                                        <  4:307022/149‑1 (MQ=255)
                                                                                                           ccTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATGGGGCGGAAGCGGGCGGCAAGGGATTCg                                       <  1:287587/149‑1 (MQ=255)
                                                                                                             tGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATGGGGCGGAAGCGGGCGGCAAGGGATTCg                                       >  3:249459/1‑147 (MQ=255)
                                                                                                               ttCGTCGGGTACACGCACGGGGCCGATGCCGGCGTCCTTGAGGCAGTGGAT‑GGGCTCATGGGGCAGGGTGGGGTCGCGCTCGCGTGCCTCGCTGGATTCTGCGATGGGGGTAAAGATGGGGCGGAAGCGGGCGGGATGTGATTCGTAGt                                   <  5:338802/149‑1 (MQ=255)
                                                                                                               ttCGACGGGTACACGCACGGCGCCGAAGCCGGCATCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATGGGGCGGAAGCGGGCGGCAAGGGATTCGTAGt                                   <  1:46958/149‑1 (MQ=255)
                                                                                                                  gACGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATGGGGCGGAAGCGGGCGGCAAGGGATTCGTAGTgg                                 <  6:175833/148‑1 (MQ=255)
                                                                                                                   aCGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATGGGGCGGAAGCGGGCGGCAAGGGATTCGTAGTGGGt                               <  7:140565/149‑1 (MQ=255)
                                                                                                                       gTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGGGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATGGGGCGGAAgcgggc                                                    >  3:23481/1‑124 (MQ=255)
                                                                                                                       gTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATGGGGCGGAAgcgggc                                                    <  4:23481/124‑1 (MQ=255)
                                                                                                                           acgcacgGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATGGGGCGGAAGCGGGCGGCAAGGGATTCGTAGTGGGTACCgg                          >  7:102112/1‑146 (MQ=255)
                                                                                                                             ggacgGCGCCGATGCCGGCGGGCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGGCACGGTGGATTGGGCGATGCGGGTAAAGATGGGGCGGAAGCGGGCGGCAAGGGATTCGTAGTGGGTACCGGTAGAc                     <  2:102863/147‑1 (MQ=255)
                                                                                                                             gcacgGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATGGGGCGGAAGCGGGCGGCAAGGGATTCGTAGTGGGTACCGGTAGa                      >  5:77906/1‑148 (MQ=255)
                                                                                                                             gcacgGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATGGGGCGGAAGCGGGCGGCAAGGGATTCGTAGTGGGTACCGGTAGa                      >  3:68363/1‑148 (MQ=255)
                                                                                                                               acgGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATGGGGCGGAAGCGGGCGGCAAGGGATTCGTAGTGGGTACCGGTAGACAg                   >  5:53056/1‑149 (MQ=255)
                                                                                                                               acgGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATGGGGCGGAAGCGGGCGGCAAGGGATTCGTAGTGGGTACCGGTAGACAg                   <  5:275975/149‑1 (MQ=255)
                                                                                                                                 ggCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATGGGGCGGAAGCGGGCGGCAAGGGATTCGTAGTGGGTACCGGTAGACAGGt                 >  5:8329/1‑149 (MQ=255)
                                                                                                                                      cGAAGCCGGCGTCCTTGAGCCACTGGATGGGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATGGGGCGGAAGCGGGCGGCAAGGGATTCGTAGTGGGTACCGGTAGACAGGTg                >  1:36754/1‑146 (MQ=255)
                                                                                                                                      cGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATGGGGCGGAAGCGGGCGGCAAGGGATTCGTAGTGGGTACCGGTAGACAGGTg                <  2:36754/145‑1 (MQ=255)
                                                                                                                                       gAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATGGGGCGGAAGCGGGCGGCAAg                                              >  6:363199/1‑114 (MQ=255)
                                                                                                                                       gAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATGGGGCGGAAGCGGGCGGCAAg                                              <  5:363199/114‑1 (MQ=255)
                                                                                                                                         aGCCGTCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATGGGGCGGAAGCGGGCGGCAAGGGATTCGTAGTGGGTACCGGTAGACAGGTGGGGCAGt         <  4:249459/149‑1 (MQ=255)
                                                                                                                                               cGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATGGGGCGGAAGCGGGCGGCAAGGGATTCGTAGTGGGTACCGGTAGACAGGTGGGGCAGTGGGGCt   <  4:68363/149‑1 (MQ=255)
                                                                                                                                                gTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATGGGGCGGAAGCGGGCGGCAAGGGATTCGTAGTGGGTACCGGTAGACAGGTGGGGCAGTGGGGCtt  >  1:220759/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
TCGACGAAAGCAAAATGCCCGCGCAGTGCCTGCGGCACATTGGAGTCGGCTTCGGCCAGTTCGATCAGCAGCTGGAACAACTGTGGCAGGGAA‑GCGCCGGCACCGCCCTGTTCGACGGGTACACGCACGGCGCCGAAGCCGGCGTCCTTGAGCCACTGGAT‑GGGCTCATGGGGCAGGGTGCGGTCGCGCTCGCGTGCCACGCTGGATTCGGCGATGCGGGTAAAGATGGGGCGGAAGCGGGCGGCAAGGGATTCGTAGTGGGTACCGGTAGACAGGTGGGGCAGTGGGGCTT  >  NC_002947/3147567‑3147858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑