Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,173,788 G→C 11.3% intergenic (+9/‑42) PP_2785 → / → PP_2786 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,173,7880GC11.3% 140.1 / 10.4 53intergenic (+9/‑42)PP_2785/PP_2786hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (23/24);  new base C (2/4);  total (25/28)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.84e-01

AGGGGATCTTCACCTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGCCGACGACATCATTCGCCAGG  >  NC_002947/3173640‑3173932
                                                                                                                                                    |                                                                                                                                                
aGGGGATCTTCACCTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACcgg                                                                                                                                                  >  5:55604/1‑149 (MQ=255)
    gATCTTCACCTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACcggtgcg                                                                                                                                              >  8:241763/1‑149 (MQ=255)
      tCTTCACCTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTAGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACcggtgcggt                                                                                                                                            >  2:95371/1‑149 (MQ=255)
        ttCACCTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGgtgt                                                                                                                                          >  1:148619/1‑149 (MQ=255)
          cACCTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACcggtgcggt                                                                                                                                            <  6:247824/145‑1 (MQ=255)
          cACCTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGgtgt                                                                                                                                          <  4:322173/147‑1 (MQ=255)
           aCCTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCAATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGc                                                                                                                                        >  5:6830/1‑148 (MQ=255)
            ccTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCg                                                                                                                                       >  4:170866/1‑148 (MQ=255)
             cTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGaa                                                                                                                                     >  3:120936/1‑149 (MQ=255)
             cTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGCACCGGtgtggtca                                                                                                                                     <  2:340712/149‑5 (MQ=255)
                cAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGCACCGGtgtggtcagaa                                                                                                                                  <  2:257085/149‑8 (MQ=255)
                          gACTGGCTCAAGTTGGGGCTCGAAGTCAATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAAt                                                                                                                        <  6:6830/149‑1 (MQ=255)
                            cTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGa                                                                                                                      <  4:120936/149‑1 (MQ=255)
                               gCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGaa                                                                                                                     >  8:160827/1‑147 (MQ=255)
                               gCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGaa                                                                                                                     <  4:180841/147‑1 (MQ=255)
                               gCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGaa                                                                                                                     >  3:180841/1‑147 (MQ=255)
                                cTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGaaaa                                                                                                                   <  3:170866/148‑1 (MQ=255)
                                cTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAg                                                                                                                  <  1:354231/149‑1 (MQ=255)
                                cTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAg                                                                                                                  <  1:348671/149‑1 (MQ=255)
                                 tCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAg                                                                                                                  >  4:299640/1‑148 (MQ=255)
                                 tCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGCACCGGtgtggtca                                                                                                                                     <  5:332700/129‑5 (MQ=255)
                                 tCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGCACCGGtgtggtca                                                                                                                                     >  6:332700/1‑125 (MQ=255)
                                                        gtgtTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATg                                                                                                                       <  3:309821/120‑1 (MQ=255)
                                                        gtgtTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATg                                                                                                                       >  4:309821/1‑120 (MQ=255)
                                                         tgtTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGGGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTc                                                                                          <  2:24493/148‑1 (MQ=255)
                                                           ttcttcGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGaa                                                                                                                     <  7:75549/119‑1 (MQ=255)
                                                           ttcttcGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGaa                                                                                                                     >  8:75549/1‑119 (MQ=255)
                                                              ttcGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGAcc                                                                                    >  8:164725/1‑149 (MQ=255)
                                                                cGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGAccc                                                                                   <  7:241763/148‑1 (MQ=255)
                                                                cGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGAcc                                                                                    <  7:160827/147‑1 (MQ=255)
                                                                     aGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCACCATGTTGCCCATCTTCAAGACCCTGACTg                                                                             >  6:299970/1‑149 (MQ=255)
                                                                         cGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGCACCGGtgtggtca                                                                                                                                     <  5:358209/89‑5 (MQ=255)
                                                                         cGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGCACCGGtgtggtca                                                                                                                                     >  6:358209/1‑85 (MQ=255)
                                                                               ccAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCAct                                                                   >  4:276188/1‑149 (MQ=255)
                                                                                 aGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCActgc                                                                 <  3:299640/149‑1 (MQ=255)
                                                                                   ttCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCActgctg                                                               <  3:276188/149‑1 (MQ=255)
                                                                                      ggTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGtt                                                             >  2:195430/1‑148 (MQ=255)
                                                                                            tACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGcgcc                                                      >  6:156333/1‑149 (MQ=255)
                                                                                                  aaCCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCACCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTgcc                                                <  5:299970/149‑1 (MQ=255)
                                                                                                     cgcgATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTgccgc                                              >  8:340827/1‑148 (MQ=255)
                                                                                                     cgcgATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTgccgc                                              >  8:19368/1‑148 (MQ=255)
                                                                                                            aGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCa                                       <  7:19368/148‑1 (MQ=255)
                                                                                                                 cgcTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGaa                                 <  1:195430/149‑1 (MQ=255)
                                                                                                                  gcTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGaaa                                <  6:55604/149‑1 (MQ=255)
                                                                                                                   cTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCTGAGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAac                               <  1:95371/149‑1 (MQ=255)
                                                                                                                         cgccgGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAAcacgcac                         <  7:164725/149‑1 (MQ=255)
                                                                                                                          gccgGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAAcacgcacg                        >  3:325058/1‑149 (MQ=255)
                                                                                                                                   cTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGCcgacga                >  3:192972/1‑148 (MQ=255)
                                                                                                                                   cTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGCcgacga                >  1:83686/1‑148 (MQ=255)
                                                                                                                                     gTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGCCGACGAcat             >  1:36247/1‑149 (MQ=255)
                                                                                                                                      ttCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGCCgacgac               <  7:340827/146‑1 (MQ=255)
                                                                                                                                      ttCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGCCGACGAcatc            <  5:156333/149‑1 (MQ=255)
                                                                                                                                         tGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGCCGACGACATCAtt         >  6:139707/1‑149 (MQ=255)
                                                                                                                                             cacaCCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGCCGACGACATCATTCGcc     <  2:83686/149‑1 (MQ=255)
                                                                                                                                               cacCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGcgccg                                                     <  7:143007/99‑1 (MQ=255)
                                                                                                                                               cacCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGcgccg                                                     >  8:143007/1‑99 (MQ=255)
                                                                                                                                                acCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGCCGACGACATCATTCGCCAgg  >  4:136041/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
AGGGGATCTTCACCTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGCCGACGACATCATTCGCCAGG  >  NC_002947/3173640‑3173932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑