Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,173,790 G→C 11.0% intergenic (+11/‑40) PP_2785 → / → PP_2786 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,173,7900GC11.0% 155.9 / 8.1 53intergenic (+11/‑40)PP_2785/PP_2786hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (23/24);  new base C (2/4);  total (25/28)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.48e-01

GATCTTCACCTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGCCGACGACATCATTCGCCAGGCCCGG  >  NC_002947/3173644‑3173937
                                                                                                                                                  |                                                                                                                                                   
gATCTTCACCTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACcggtgcg                                                                                                                                                   >  8:241763/1‑149 (MQ=255)
  tCTTCACCTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTAGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACcggtgcggt                                                                                                                                                 >  2:95371/1‑149 (MQ=255)
    ttCACCTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGgtgt                                                                                                                                               >  1:148619/1‑149 (MQ=255)
      cACCTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACcggtgcggt                                                                                                                                                 <  6:247824/145‑1 (MQ=255)
      cACCTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGgtgt                                                                                                                                               <  4:322173/147‑1 (MQ=255)
       aCCTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCAATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGc                                                                                                                                             >  5:6830/1‑148 (MQ=255)
        ccTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCg                                                                                                                                            >  4:170866/1‑148 (MQ=255)
         cTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGaa                                                                                                                                          >  3:120936/1‑149 (MQ=255)
         cTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGCACCGGtgtggtca                                                                                                                                          <  2:340712/149‑5 (MQ=255)
            cAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGCACCGGtgtggtcagaa                                                                                                                                       <  2:257085/149‑8 (MQ=255)
                      gACTGGCTCAAGTTGGGGCTCGAAGTCAATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAAt                                                                                                                             <  6:6830/149‑1 (MQ=255)
                        cTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGa                                                                                                                           <  4:120936/149‑1 (MQ=255)
                           gCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGaa                                                                                                                          >  8:160827/1‑147 (MQ=255)
                           gCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGaa                                                                                                                          <  4:180841/147‑1 (MQ=255)
                           gCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGaa                                                                                                                          >  3:180841/1‑147 (MQ=255)
                            cTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGaaaa                                                                                                                        <  3:170866/148‑1 (MQ=255)
                            cTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAg                                                                                                                       <  1:354231/149‑1 (MQ=255)
                            cTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAg                                                                                                                       <  1:348671/149‑1 (MQ=255)
                             tCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAg                                                                                                                       >  4:299640/1‑148 (MQ=255)
                             tCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGCACCGGtgtggtca                                                                                                                                          <  5:332700/129‑5 (MQ=255)
                             tCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGCACCGGtgtggtca                                                                                                                                          >  6:332700/1‑125 (MQ=255)
                                                    gtgtTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATg                                                                                                                            <  3:309821/120‑1 (MQ=255)
                                                    gtgtTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATg                                                                                                                            >  4:309821/1‑120 (MQ=255)
                                                     tgtTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGGGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTc                                                                                               <  2:24493/148‑1 (MQ=255)
                                                       ttcttcGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGaa                                                                                                                          <  7:75549/119‑1 (MQ=255)
                                                       ttcttcGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGaa                                                                                                                          >  8:75549/1‑119 (MQ=255)
                                                          ttcGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGAcc                                                                                         >  8:164725/1‑149 (MQ=255)
                                                            cGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGAccc                                                                                        <  7:241763/148‑1 (MQ=255)
                                                            cGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGAcc                                                                                         <  7:160827/147‑1 (MQ=255)
                                                                 aGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCACCATGTTGCCCATCTTCAAGACCCTGACTg                                                                                  >  6:299970/1‑149 (MQ=255)
                                                                     cGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGCACCGGtgtggtca                                                                                                                                          >  6:358209/1‑85 (MQ=255)
                                                                     cGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGCACCGGtgtggtca                                                                                                                                          <  5:358209/89‑5 (MQ=255)
                                                                           ccAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCAct                                                                        >  4:276188/1‑149 (MQ=255)
                                                                             aGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCActgc                                                                      <  3:299640/149‑1 (MQ=255)
                                                                               ttCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCActgctg                                                                    <  3:276188/149‑1 (MQ=255)
                                                                                  ggTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGtt                                                                  >  2:195430/1‑148 (MQ=255)
                                                                                        tACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGcgcc                                                           >  6:156333/1‑149 (MQ=255)
                                                                                              aaCCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCACCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTgcc                                                     <  5:299970/149‑1 (MQ=255)
                                                                                                 cgcgATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTgccgc                                                   >  8:19368/1‑148 (MQ=255)
                                                                                                 cgcgATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTgccgc                                                   >  8:340827/1‑148 (MQ=255)
                                                                                                        aGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCa                                            <  7:19368/148‑1 (MQ=255)
                                                                                                             cgcTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGaa                                      <  1:195430/149‑1 (MQ=255)
                                                                                                              gcTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGaaa                                     <  6:55604/149‑1 (MQ=255)
                                                                                                               cTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCTGAGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAac                                    <  1:95371/149‑1 (MQ=255)
                                                                                                                     cgccgGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAAcacgcac                              <  7:164725/149‑1 (MQ=255)
                                                                                                                      gccgGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAAcacgcacg                             >  3:325058/1‑149 (MQ=255)
                                                                                                                               cTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGCcgacga                     >  3:192972/1‑148 (MQ=255)
                                                                                                                               cTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGCcgacga                     >  1:83686/1‑148 (MQ=255)
                                                                                                                                 gTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGCCGACGAcat                  >  1:36247/1‑149 (MQ=255)
                                                                                                                                  ttCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGCCgacgac                    <  7:340827/146‑1 (MQ=255)
                                                                                                                                  ttCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGCCGACGAcatc                 <  5:156333/149‑1 (MQ=255)
                                                                                                                                     tGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGCCGACGACATCAtt              >  6:139707/1‑149 (MQ=255)
                                                                                                                                         cacaCCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGCCGACGACATCATTCGcc          <  2:83686/149‑1 (MQ=255)
                                                                                                                                           cacCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGcgccg                                                          <  7:143007/99‑1 (MQ=255)
                                                                                                                                           cacCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGcgccg                                                          >  8:143007/1‑99 (MQ=255)
                                                                                                                                            acCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGCCGACGACATCATTCGCCAgg       >  4:136041/1‑149 (MQ=255)
                                                                                                                                                 tgcggtgTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGCCGACGACATCATTCGCCAGGCCCgg  <  4:325058/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GATCTTCACCTACAAACCGGTGGACTGGCTCAAGTTGGGGCTCGAAGTCGATGTGTTCTTCGGCAAGCCCGACCGCCAGTTCGGTGCGTACGACAACCGCGATCAGGTGCGCTTGTCCGCCGGTTATCTGTTCTGACCACACCGGTGCGGTGTGCGAACCACTCAAACAATGAAAAGGAAATCGCCATGTTGCCCATCTTCAAGACCCTGACTGTAAGCGCACTGCTGTTGAGCGCCGCTGCCGCCATGGCAGCTGAAACACGCACGGCCGACGACATCATTCGCCAGGCCCGG  >  NC_002947/3173644‑3173937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑