Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,179,890 C→G 5.2% A262P (GCG→CCG)  PP_2790 ← sigma‑54 dependent sensory box protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,179,8900CG 5.2% 250.5 / 3.7 77A262P (GCG→CCG) PP_2790sigma‑54 dependent sensory box protein
Reads supporting (aligned to +/- strand):  ref base C (37/36);  new base G (2/2);  total (39/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.95e-01

GGCACGTTGCACAACAGTGATGTCCTGGCAGGTCAGGACCAGGCCGGTACGTTCGCCGTTCTCCAGGATAGGCAGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTGCGCAAGGGCAGGGTTGAGCGACTGCACCACGCCTT‑GGTTGTCGACCGCTGCTACGCCGGCGGGGATA  >  NC_002947/3179744‑3180035
                                                                                                                                                  |                                                                                                                                                  
ggCACGTTGCACAACAGTGATGTCCTGGCAGGTCAGGACCAGGCCGGTACGTTCGCCGTTCTCCAGGATAGGCAGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCct                                                                                                                                                  <  5:324903/149‑1 (MQ=255)
   aCGTTGCACAACAGTGATGTCCTGGCAGGTCAGGACCAGGCCGGTACGTTCGCCGTTCTCCAGGATAGGCAGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCctgct                                                                                                                                               <  8:23448/149‑1 (MQ=255)
     gTTGCACAACAGTGATGTCCTGGCAGGTCAGGACCAGGCCGGTACGTTCGCCGTTCTCCAGGATAGGCAGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCctgctg                                                                                                                                              >  7:215524/1‑148 (MQ=255)
     gTTGCACAACAGTGATGTCCTGGCAGGTCAGGACCAGGCCGGTACGTTCGCCGTTCTCCAGGATAGGCAGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCtgctgc                                                                                                                                             <  6:141561/149‑1 (MQ=255)
     gTTGCACAACAGTGATGTCCTGGCAGGTCAGGACCAGGCCGGTACGTTCGCCGTTCTCCAGGATAGGCAGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCtgctgc                                                                                                                                             <  8:288314/149‑1 (MQ=255)
               aGTGATGTCCTGGCAGGTCAGGACCAGGCCGGTACGTTCGCCGTTCTCCAGGATAGGCAGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGtt                                                                                                                                   >  2:262930/1‑149 (MQ=255)
                     gTCCTGGCAGGTCAGGACCAGGCCGGTACGTTCGCCGTTCTCCAGGATAGGCAGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCggg                                                                                                                              >  8:154501/1‑148 (MQ=255)
                         tGGCAGGTCAGGACCAGGCCGGTACGTTCGCCGTTCTCCAGGATAGGCAGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAg                                                                                                                         >  4:65254/1‑149 (MQ=255)
                         tGGCAGGTCAGGACCAGGCCGGTACGTTCGCCGTTCTCCAGGATAGGCAGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCAGGCGCTGCAggaag                                                                                                                                       >  8:108015/1‑132 (MQ=255)
                         tGGCAGGTCAGGACCAGGCCGGTACGTTCGCCGTTCTCCAGGATAGGCAGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCAGGCGCTGCAggaag                                                                                                                                       <  7:108015/135‑4 (MQ=255)
                                tCAGGACCAGGCCGGTACGTTCGCCGTTCTCCAGGATAGGCAGTAAATTGATCACCACGGCGTGCGAACCGAGCCCCATCACGCGGTGTTCTTCACCCGTGCCTTCCTGCAGCGCATGCTCCAGGTCCAGTTCCGGGCACAGctgctg                                                                                                                   >  5:177879/1‑148 (MQ=255)
                                  aGGACCAGGCCGGTACGTTCGCCGTTCTCCAGGATAGGCAGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCa                                                                                                                 <  3:65254/148‑1 (MQ=255)
                                    gACCAGGCCGGTACGTTCGCCGTTCTCCAGGATAGGCAGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGcg                                                                                                              >  8:153764/1‑149 (MQ=255)
                                       cAGGCCGGTACGTTCGCCGTTCTCCAGGATAGGCAGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGcggcc                                                                                                           <  8:215524/149‑1 (MQ=255)
                                       cAGGCCGGTACGTTCGCCGTTCTCCAGGATAGGCAGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGcggcc                                                                                                           >  3:98017/1‑149 (MQ=255)
                                       cAGGCCGGTACGTTCGCCGTTCTCCAGGATAGGCAGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTCCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGcggcc                                                                                                           >  8:331967/1‑149 (MQ=255)
                                        aGGCCGGTACGTTCGCCGTTCTCCAGGATAGGCAGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAg                                                                                                                                     <  3:26359/122‑1 (MQ=255)
                                        aGGCCGGTACGTTCGCCGTTCTCCAGGATAGGCAGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAg                                                                                                                                     >  4:26359/1‑122 (MQ=255)
                                         ggCCGGTACGTTCGCCGTTCTCCAGGATAGGCAGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGcggccgg                                                                                                         >  7:140611/1‑149 (MQ=255)
                                             ggTACGTTCGCCGTTCTCCAGGATAGGCAGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCa                                                                                                     <  7:153764/149‑1 (MQ=255)
                                                   ttCGCCGTTCTCCAGGATAGGCAGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAgcgctg                                                                                               >  2:175024/1‑149 (MQ=255)
                                                   ttCGCCGTTCTCCAGGATAGGCAGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAgcgctg                                                                                               >  5:158871/1‑149 (MQ=255)
                                                      gCCGTTCTCCAGGATAGGCAGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAgcgctgcgc                                                                                            >  8:65469/1‑149 (MQ=255)
                                                       ccGTTCACCAGGATAGGCAGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAgcgctgcgc                                                                                            >  1:198708/1‑148 (MQ=255)
                                                              ccAGGATAGGCAGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCgg                                                                                     >  7:23348/1‑148 (MQ=255)
                                                               ccggATAGGCAGTAAATTGTTCAGCTCGTCGTGCGAACCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCgg                                                                                     <  6:177879/145‑1 (MQ=255)
                                                               cAGGATAGGCAGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCAGGCGCTGCAggaa                                                                                                                                        >  7:18530/1‑94 (MQ=255)
                                                               cAGGATAGGCAGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCAGGCGCTGCAggaa                                                                                                                                        <  8:18530/96‑3 (MQ=255)
                                                               cAGGATAGGCAGTAAATTGCTCACCACGGCGTGCGAGCCGAGCAGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCgg                                                                                     >  6:339047/1‑147 (MQ=255)
                                                                aGGATAGGCAGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAg                                                                                  <  7:65469/149‑1 (MQ=255)
                                                                   aTAGGCAGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTc                                                                               <  2:46494/149‑1 (MQ=255)
                                                                     aGGCAGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTc                                                                               >  6:27798/1‑147 (MQ=255)
                                                                       gCAGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCa                                                                                   <  4:94687/141‑1 (MQ=255)
                                                                       gCAGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCa                                                                                   >  3:94687/1‑141 (MQ=255)
                                                                       gCAGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCcagc                                                                           >  5:210946/1‑149 (MQ=255)
                                                                       gCAGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCcagc                                                                           >  6:343616/1‑149 (MQ=255)
                                                                         aGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTc                                                                                                                                  <  8:32417/92‑1 (MQ=255)
                                                                         aGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTc                                                                                                                                  >  7:32417/1‑92 (MQ=255)
                                                                         aGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCcagcag                                                                         >  5:75586/1‑149 (MQ=255)
                                                                         aGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCACCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCcagcag                                                                         >  7:294898/1‑149 (MQ=255)
                                                                           tAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCt                                                                       <  1:175024/149‑1 (MQ=255)
                                                                            aaaTTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTg                                                                      >  5:95190/1‑149 (MQ=255)
                                                                                  cTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCATCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTGCGCaa                                                                 <  1:262930/148‑1 (MQ=255)
                                                                                   tCACCACGGCGTGCGCGCCGAGCCGCATCACGCGGTGTTCTGCCCCCGGGCCTTCCTGCAGCGCCTGCGGCCGGTCCAGGGCCGGGCCCCGCTGCGGCAGCGGCCGGCCCCGCGCTGCGCGAACCGGCAGGTCCAGCAGCTGCGCAAgg                                                               >  5:264914/1‑149 (MQ=255)
                                                                                        actgCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTGCGCAAGGGCAgg                                                          <  4:206643/146‑1 (MQ=255)
                                                                                          ggCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTGCGCAAGGGCAGGGt                                                        >  7:92372/1‑149 (MQ=255)
                                                                                           gCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTgcgc                                                                   <  3:348866/137‑1 (MQ=255)
                                                                                           gCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTgcgc                                                                   >  4:348866/1‑137 (MQ=255)
                                                                                           gCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTGCGCAAGGGCAGGGtt                                                       <  8:23348/149‑1 (MQ=255)
                                                                                           gCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTGCGCAAGGGCAGGGtt                                                       <  8:140611/149‑1 (MQ=255)
                                                                                            cGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTGCGCAAGGGCAGGGTTg                                                      <  4:98017/149‑1 (MQ=255)
                                                                                                cgagccgagcCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTGCGCAAGGGCAGGGTTGAGc                                                   <  6:158871/148‑1 (MQ=255)
                                                                                                cgagccgagcCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTGCGCAAGGGCAGGGTTGAGc                                                   <  6:72383/148‑1 (MQ=255)
                                                                                                cgagccgagcCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTGCGCAAGGGCAGGGTTGAGCg                                                  <  7:154501/149‑1 (MQ=255)
                                                                                                         ccgCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTGCGCaa                                                                 <  3:72086/125‑1 (MQ=255)
                                                                                                         ccgCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTGCGCaa                                                                 >  4:72086/1‑125 (MQ=255)
                                                                                                            cATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTGCGCAAGGGCAGGGTTGAGCGACTGCACCACGc                                      <  7:331967/149‑1 (MQ=255)
                                                                                                               cACGCGGTTCTCTGCACCCGGGCCTTCCTCCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCCGCGCTAACCGGCACGTCCAGCAGCTGCGCAAGGGCAGGGTTGAGCGACTGCACCACCCCtt                                   <  8:92936/149‑1 (MQ=255)
                                                                                                                aCGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTGCGCAAg                                                                <  7:252156/119‑1 (MQ=255)
                                                                                                                aCGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTGCGCAAg                                                                >  8:252156/1‑119 (MQ=255)
                                                                                                                 cgcgTTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTGCGCAAGGGCAGGGTTGAGCGACTGCACCACGCCTT‑gg                                <  6:210946/149‑1 (MQ=255)
                                                                                                                   cgGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTGCGCAAGGGCAGGGTTGAGCGACTGCACCACGCCTT‑GGtt                              <  2:198708/149‑1 (MQ=255)
                                                                                                                          cttcACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTGCGCAAGGGCAGGGTTGAGCGACTGCACCACGCCTT‑GGTTGTCGAcc                       >  2:27427/1‑149 (MQ=255)
                                                                                                                           ttcACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTGCGCAAGGGCAGGGTTGAGCGACTGCACCACGCCTT‑GGTTGTCGACCg                      >  5:59112/1‑149 (MQ=255)
                                                                                                                           ttcACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTGCGCAAGGGCAGGGTTGAGCGACTGCACCACGCCTT‑GGTTGTCGACCg                      >  2:321822/1‑149 (MQ=255)
                                                                                                                             cACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTGCGCAAGGGCAGGGTTGAGCGACTGCACCACGCCTT‑GGTTGTCGACCgc                     <  5:339047/148‑1 (MQ=255)
                                                                                                                              aCCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTGCGCAAGGGCAGGGTTGAGCGACTGCACCACGCCTT‑GGTTGTCGACCgctg                   >  1:292933/1‑149 (MQ=255)
                                                                                                                                ccGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTGCGCAAGGGCAgg                                                          >  4:119257/1‑109 (MQ=255)
                                                                                                                                ccGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTGCGCAAGGGCAgg                                                          <  3:119257/109‑1 (MQ=255)
                                                                                                                                ccGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTGCGCAAGGGCAGGGTTGAGCGACTGCACCACGCCTT‑GGTTGTCGACCgctgc                  >  1:301212/1‑148 (MQ=255)
                                                                                                                                            gCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTGCGCAAGGGCAGGGTTGAGCGACTGCACCACGCCTT‑GGTTGTCGACCGCTGCTACGCCGGCggg      >  5:9319/1‑148 (MQ=255)
                                                                                                                                             cAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTGCGCAAGGGCAGGGTTGAGCGACTGCACCACGCCTTCGGTTGTCGACCGCTGCTACGCCGGCgggg     <  1:27427/149‑1 (MQ=255)
                                                                                                                                             cAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTGCGCAAGGGCAGGGTTGAGCGACTGCACCACGCCTT‑GGTTGTCGACCGCTGCTACGCCGGCgggg     <  8:294898/148‑1 (MQ=255)
                                                                                                                                             cAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTGCGCAAGGGCAGGGTTGAGCGACTGCACCACGCCTT‑GGTTGTCGACCGCTGCTACGCCGGCgggg     <  5:27798/148‑1 (MQ=255)
                                                                                                                                             cAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTGCGCAAGGGCAGGGTTGAGCGACTGCACCACGCCTT‑GGTTGTCGACCGCTGCTACGCCGGCggg      <  1:321822/147‑1 (MQ=255)
                                                                                                                                             cAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTGCGCAAGGGCAGGGTTGAGCGACTGCACCACGCCTT‑GGTTGTCGACCGCTGCTACGCCGGCggg      <  6:75586/147‑1 (MQ=255)
                                                                                                                                              aGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTGCGCAAGGGCAGGGTTGAGCGACTGCACCACGCCTT‑GGTTGTCGACCGCTGCTACGCCGGCGGGGa    >  4:195769/1‑148 (MQ=255)
                                                                                                                                               gcgcCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTGCGCAAGGGCAGGGTTGAGCGACTGCACCACGCCTT‑GGTTGTCGACCGCTGCTACGCCGGCGGGGata  >  5:166842/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GGCACGTTGCACAACAGTGATGTCCTGGCAGGTCAGGACCAGGCCGGTACGTTCGCCGTTCTCCAGGATAGGCAGTAAATTGCTCACCACGGCGTGCGAGCCGAGCCGCATCACGCGGTTTTCTTCACCCGTGCCTTCCTGCAGCGCCTGCTGCAGGTCCAGTTCCGGGCACAGCTGCTGCAGCGGCCGGCCCAGCGCTGCGCTAACCGGCAGGTCCAGCAGCTGCGCAAGGGCAGGGTTGAGCGACTGCACCACGCCTT‑GGTTGTCGACCGCTGCTACGCCGGCGGGGATA  >  NC_002947/3179744‑3180035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑