Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	284,586	T→A	15.4%	intergenic (+33/+30)	betT‑II → / ← atsK	choline/carnitine/betaine transporter/alpha‑ketoglutarate‑dependent sulfonate dioxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	284,586	0	T	A	15.4%	139.1 / 17.1	65	intergenic (+33/+30)	betT‑II/atsK	choline/carnitine/betaine transporter/alpha‑ketoglutarate‑dependent sulfonate dioxygenase
Reads supporting (aligned to +/- strand):  ref base T (30/25);  new base A (5/5);  total (35/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-01

GCAGCCAGAACTATGACCTGGTGGGCTACAGCAAGGAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTGGGTCACGCGGTTGTCCCAGAACGCCACGTCATTCTCC  >  NC_002947/284450‑284733                                                                                                                                          |                                                                                                                                                     gCAGCCAGAACTATGACCTGGTGGGCTACAGCAAGGAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGCCCGCGAaga                                                                                                                                          <  1:274100/149‑1 (MQ=255) gCAGCCAGAACTATGACCTGGTGGGCTACAGCAAGGAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGT‑‑CTGGCCTCTTCGCGGGCAAGCCCGCGaa                                                                                                                                            >  8:97805/1‑146 (MQ=255) gCAGCCAGAACTATGACCTGGTGGGCTACAGCAAGGAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGT‑‑CTGGCCTCTTCGCGGGCAAGCCCGCGAaga                                                                                                                                          <  7:97805/148‑1 (MQ=255)  cAGCCAGAACTATGACCTGGTGGGCTACAGCAAGGAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGT‑CCTGGCCTCTTCTCGGGCTTGCCCGCGAagag                                                                                                                                         <  4:246121/149‑1 (MQ=255)  cAGCCAGAACTATGACCTGGTGGGCTACAGCAAGGAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGCCCGCGAagag                                                                                                                                         <  8:31549/149‑1 (MQ=255)  cAGCCAGAACTATGACCTGGTGGGCTACAGCAAGGAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCAAGCCCGCGAagag                                                                                                                                         <  1:230202/149‑1 (MQ=255)      cAGAACTATGACCTGGTGGGCTACAGCAAGGAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTGCCTGCACCTGGTCCGCTGACCAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCAAGCCCGCGAAGAGGCCa                                                                                                                                     <  7:289927/149‑1 (MQ=255)      cAGAACTATGACCTGGTGGGCTACAGCAAGGAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGccc                                                                                                                                                 <  4:107106/137‑1 (MQ=255)      cAGAACTATGACCTGGTGGGCTACAGCAAGGAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGccc                                                                                                                                                 >  3:107106/1‑137 (MQ=255)      cAGAACTATGACCTGGTGGGCTACAGCAAGGAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGCCCGCGaa                                                                                                                                            <  3:4301/142‑1 (MQ=255)      cAGAACTATGACCTGGTGGGCTACAGCAAGGAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGCCCGCGaa                                                                                                                                            >  4:4301/1‑142 (MQ=255)       aGAACTATGACCTGGTGGGCTACAGCAAGGAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGTAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGA‑CCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGc                                                                                                                                       >  4:50137/1‑146 (MQ=255)            tATGACCTGGTGGGCTACAGCAAGGAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAgg                                                                                                                               >  5:190480/1‑149 (MQ=255)            tATGACCTGGTGGGCTACAGCAAGGAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAgg                                                                                                                               >  5:262905/1‑149 (MQ=255)                   tggtggGCTACAGCAAGGAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTGCTGT‑CCTGGCCTCTTCGCGGGCAAGCCCGCGaa                                                                                                                                            >  1:294947/1‑129 (MQ=255)                   tggtggGCTACAGCAAGGAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCAAGCCCGCGaa                                                                                                                                            <  2:294947/129‑1 (MQ=255)                    ggtggGCTACAGCAAGGAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACC‑Gc                                                                                                                       >  1:20239/1‑148 (MQ=255)                         gCTACAGCAAGGAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGa                                                                                                                   >  3:235741/1‑147 (MQ=255)                            aCAGCAAGGAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGt                                                                                                              >  3:335204/1‑149 (MQ=255)                                      gCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCaa                                                                                                    <  4:335204/149‑1 (MQ=255)                                        aGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAg                                                                                                   >  1:351992/1‑148 (MQ=255)                                         gATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGCCCGCGAAGGGGCCAGACAGGCAACC‑GCTAGAAAGGTTCATCCCCAGGGa                                                                                                 >  8:229073/1‑149 (MQ=255)                                         gATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGa                                                                                                 >  3:212037/1‑149 (MQ=255)                                           tcatcaACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATg                                                                                               <  6:262905/149‑1 (MQ=255)                                           tcatcaACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATg                                                                                               <  2:20239/149‑1 (MQ=255)                                                  cGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAATGATGGTCGccc                                                                                        >  1:155941/1‑149 (MQ=255)                                                   gACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCg                                                                                       <  4:235741/149‑1 (MQ=255)                                                          tCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCCGACAGGCCACC‑GCGAGAAAGGt                                                                                                              >  1:346784/1‑119 (MQ=255)                                                          tCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGGCAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCAt                                                                                 >  8:250812/1‑148 (MQ=255)                                                          tCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCAt                                                                                 >  2:76379/1‑148 (MQ=255)                                                             aCCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCAcc                                                                             >  6:302252/1‑149 (MQ=255)                                                                  tACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACccgccg                                                                        >  1:248462/1‑149 (MQ=255)                                                                     gAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGtt                                                                     <  2:19432/149‑1 (MQ=255)                                                                          gcaattGCAGTACGTGCACCTGGTCCGCTGACAATGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGCCGGCTAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTtcggtc                                                                <  2:155941/144‑1 (MQ=255)                                                                            acaTGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTtcggtcgg                                                              <  6:190480/149‑1 (MQ=255)                                                                                         ctaCCTGGGCCGCTGACCAAGCTCTCCTGT‑CGTGGCCTCTTCGCGGGCTGGCCCGCTATTTGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCAcc                                                  <  2:193965/146‑1 (MQ=255)                                                                                            ccTGGTCCGCTGACCAAGCTCTCCTGTGCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCAcc                                                  >  1:193965/1‑146 (MQ=255)                                                                                            ccTGGTCCGCTGACCAAGCTCTCCTGTGCCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCAcc                                                  >  1:199526/1‑146 (MQ=255)                                                                                                   cGCTGACCAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCt                                        <  4:212037/148‑1 (MQ=255)                                                                                                   cGCTGACCAAGCTCTCCTGA‑CCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTg                                       <  3:50137/149‑1 (MQ=255)                                                                                                    gCTGACCAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTgg                                      <  1:76379/149‑1 (MQ=255)                                                                                                    gCTGACCAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTgg                                      >  3:268242/1‑149 (MQ=255)                                                                                                     cTGACCATGCTCTCCTTT‑CCTGGCCTCTTCGCGGGATTGCCCGCTAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTggg                                     <  2:351992/149‑1 (MQ=255)                                                                                                     cTGACCAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTggg                                     <  2:248462/149‑1 (MQ=255)                                                                                                         ccAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTGGGTCAc                                 >  1:233668/1‑149 (MQ=255)                                                                                                          cAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTg                                       <  8:150138/142‑1 (MQ=255)                                                                                                          cAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTg                                       >  7:150138/1‑142 (MQ=255)                                                                                                            aGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTGGGTCAcgcg                              <  6:80313/149‑1 (MQ=255)                                                                                                                     gt‑cctgGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTGGGTCACGCGGTTGTCCCa                     >  6:201926/1‑149 (MQ=255)                                                                                                                      t‑cctgGCCGCTTCGCGGGGTTGCCCGCGAATATGCCAGACAGGCAACC‑GCTAGAAACGTGCATCCCCAACGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTGGGTCACGCGGTTGTCCCa                     <  7:229073/148‑1 (MQ=255)                                                                                                                           ggCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCAt                                                                                 >  6:270581/1‑84 (MQ=255)                                                                                                                           ggCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCAt                                                                                 <  5:270581/84‑1 (MQ=255)                                                                                                                              ctctTCGCGGGCAAGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTGGGTCACGCGGTTGTCCCAGAACGCCa             >  7:281857/1‑149 (MQ=255)                                                                                                                               tctTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCACCC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTGGGTCACGCGGTTGTCCCAGAACGCCAc            >  8:127/1‑149 (MQ=255)                                                                                                                               tctTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGTCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTg                                       >  7:303289/1‑122 (MQ=255)                                                                                                                               tctTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGTCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTg                                       <  8:303289/122‑1 (MQ=255)                                                                                                                               tctTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTGGGTCACGCGGTTGTCCCAGAACGCCAc            >  2:181648/1‑149 (MQ=255)                                                                                                                               tctTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGCTTCGGTAGGTAATCATCCACCGAAAAATGCTGGGTCACGCGGTTGTCCCAGAACGCCAc            >  5:158886/1‑149 (MQ=255)                                                                                                                               tctTCGCGGGCAAGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTGGGTAACGCGGTTGTCCCAGAACGCCAc            >  7:73732/1‑149 (MQ=255)                                                                                                                                 ttCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTGGGTCACGCGGTTGTCCCAGAACGCCAc            <  2:233668/147‑1 (MQ=255)                                                                                                                                 ttCGCGGGCAAGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTGGGTCACGCGGTTGTCCCAGAACGCCACGt          >  3:237721/1‑149 (MQ=255)                                                                                                                                     cgGGCAAGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTGGGTCACGCGGTTGTCCCAGAACGCCACGTCAtt      <  8:281857/149‑1 (MQ=255)                                                                                                                                         cTTGCCCGCGAAGAGGCCAGACAGGCAACCGGCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTGGGTCACGCGGTTGTCCCAGAACGCCACGTCATtctc   >  1:32578/1‑149 (MQ=255)                                                                                                                                         cTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTGGGTCACGCGGTTGTCCCAGCACGCCACGTCATTCTcc  >  4:167084/1‑149 (MQ=255)                                                                                                                                         cTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTGGGTCACGCGGTTGTCCCAGAACGCCACGTCATTCTcc  <  5:201926/149‑1 (MQ=255)                                                                                                                                          |                                                                                                                                                     GCAGCCAGAACTATGACCTGGTGGGCTACAGCAAGGAGCAGATCATCAACGACATCCTCGACCAGTACGAGCGGCACATGCAGTACCTGCACCTGGTCCGCTGACCAAGCTCTCCTGT‑CCTGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGCCAGACAGGCAACC‑GCTAGAAAGGTGCATCCCCAAGGATGGTCGCCCGGTGCATCACCCGCCGGTTCGGTCGGTAATCATCCACCGCAAAATGCTGGGTCACGCGGTTGTCCCAGAACGCCACGTCATTCTCC  >  NC_002947/284450‑284733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑