Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,224,605 C→T 7.0% E272K (GAA→AAA)  mexT ← transcriptional regulator MexT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,224,6050CT 7.0% 166.5 / 2.1 57E272K (GAA→AAA) mexTtranscriptional regulator MexT
Reads supporting (aligned to +/- strand):  ref base C (24/29);  new base T (2/2);  total (26/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTTGCTCACGAGGTCGTGCGATTTGATCTCAGTGGAAGCCTGTCACAAGCTATCGGGATCCCCAAAGAACATCTGTATCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCCGCAAGCCGCCGGCGGCCGTCAGTGCATCCGCCGT‑GTAGTCCGGTACCGTAGCCACGATATCGGTGCCTGCCAGCAAGCTCCCCAGCCCGTTGAACTGCGGTACCGCCAGCA  >  NC_002947/3224461‑3224751
                                                                                                                                                |                                                                                                                                                   
aTTTGCTCACGAGGTCGTGCGATTTGATCTCAGTGGAAGCCTGTCACAAGCTATCGGGATCCCCAAAGAACATCTGTATCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGaaa                                                                                                                                                 >  8:50147/1‑149 (MQ=255)
     cTCACGAGGTCGTGCGATTTGATCTCAGTGGAAGCCTGTCACAAGCTATCGGGATCCCCAAAGAACATCTGTATCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTgc                                                                                                                                             <  7:50147/148‑1 (MQ=255)
      tCACGAGGTCGTGCGATTTGATCTCAGTGGAAGCCTGTCACAAGCTATCGGGATCCCCAAAGAACATCTGTATCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTTCGAACTgca                                                                                                                                            >  3:192676/1‑147 (MQ=255)
        aCGAGGTCGTGCGATTTGATCTCAGTGGAAGCCTGTCACAAGCTATCGGGATCCCCAAAGAACATCTGTATCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTTCGAACTGcaca                                                                                                                                          <  4:192676/148‑1 (MQ=255)
          gAGGTCGTGCGATTTGATCTCAGTGGAAGCCTGTCACAAGCTATCGGGATCCCCAAAGAACATCTGTATCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACgg                                                                                                                                       <  7:62901/149‑1 (MQ=255)
          gAGGGCGTGCGATTTGATCTCAGTGGAAGCCTGTCACAAGCTATCGGGATCCCCAAAGAACATCTGTATCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACgg                                                                                                                                       <  2:24588/149‑1 (MQ=255)
              tCGTGCGATTTGATCTCAGTGGAAGCCTGTCACAAGCTATCGGGATCCCCAAAGAACATCTGTATCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTAt                                                                                                                                   <  3:343063/149‑1 (MQ=255)
                gTGCGATTTGATCTCAGTGGAAGCCTGTCACAAGCTATCGGGATCCCCAAAGAACATCTGTATCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATc                                                                                                                                  <  4:186648/148‑1 (MQ=255)
                    gATTTGATCTCAGTGGAAGCCTGTCACAAGCTATCGGGATCCCCAGAGAACATCTGTATCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAg                                                                                                                             <  5:83235/149‑1 (MQ=255)
                      tttGATCTCAGTGGAAGCCTGTCACAAGCTATCGGGATCCCCAAAGAACATCTGTATCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGt                                                                                                                           <  2:260622/149‑1 (MQ=255)
                           tctcAGTGGAAGCCTGTCACAAGCTATCGGGATCCCCAAAGAACATCTGTATCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTTCGAACTGcaca                                                                                                                                          <  8:314480/129‑1 (MQ=255)
                           tctcAGTGGAAGCCTGTCACAAGCTATCGGGATCCCCAAAGAACATCTGTATCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTTCGAACTGcaca                                                                                                                                          >  7:314480/1‑129 (MQ=255)
                            ctcAGTGGAAGCCTGTCACAAGCTATCGGGATCCCCAAAGAACATCTGTATCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTgc                                                                                                                                             >  8:272578/1‑125 (MQ=255)
                            ctcAGTGGAAGCCTGTCACAAGCTATCGGGATCCCCAAAGAACATCTGTATCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTgc                                                                                                                                             <  7:272578/125‑1 (MQ=255)
                               aGTGGAAGCCTGTCACAAGCTATCGGGATCCCCAAAGAACATCTGTATCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGcc                                                                                                                   <  6:61888/148‑1 (MQ=255)
                                gTGGAAGCCTGTCACAAGCTATCGGGATCCCCAAAGAACATCTGTATCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCCg                                                                                                                 >  7:38121/1‑149 (MQ=255)
                                                  cTATCGGGATCCCCAAAGAACATCTGTATCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGGTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCCGCAAGCCGCCGGCGGCCGt                                                                                               >  5:263/1‑149 (MQ=255)
                                                   tATCGGGATCCCCAAAGAACATCTGTATCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCCGCAAGCCGCCGGCGGCCGt                                                                                               >  5:361521/1‑148 (MQ=255)
                                                     tCGGGATCCCCAAAGAACATCTGTATCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAg                                                                                                                             >  3:335366/1‑116 (MQ=255)
                                                     tCGGGATCCCCAAAGAACATCTGTATCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAg                                                                                                                             <  4:335366/116‑1 (MQ=255)
                                                     tCGGGATCCCCAAAGAACATCTGTATCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCCGCAAGCCGCCGGCGGCCGTCa                                                                                             <  8:38121/148‑1 (MQ=255)
                                                     tCGGGATCCCCAAAGAACATCTGTATCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCCGCAAGCCGCCGGCGGCCGTCAg                                                                                            >  2:327245/1‑149 (MQ=255)
                                                       gggATCCCCAAAGAACATCTGTATCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCCGCAAGCCGCCGGCGGCCGTCAGTg                                                                                          >  2:122668/1‑149 (MQ=255)
                                                       gggATCCCCAAAGAACATCTGTATCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCCGCAAGCCGCCGGCGGCCGTCAGTg                                                                                          >  8:337343/1‑149 (MQ=255)
                                                        ggATCCCCAAAGAACATCTGTATCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCCGCAAGCCGCCGGCGGCCGTCAGTg                                                                                          >  6:254147/1‑148 (MQ=255)
                                                        ggATCCCCAAACAACATCTGTATCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCCGCAAGCCGCCGGCGGCCGTCAGTGc                                                                                         <  7:295823/149‑1 (MQ=255)
                                                              ccAAAGAACATCTGTATCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCTGCAAGCCGCCGGCGGcc                                                                                                 >  7:295070/1‑135 (MQ=255)
                                                              ccAAAGAACATCTGTATCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCTGCAAGCCGCCGGCGGcc                                                                                                 <  8:295070/135‑1 (MQ=255)
                                                               cAAAGAACATCTGTATCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCCGCAAGCCGCCGGCGGCCGt                                                                                               >  5:238391/1‑136 (MQ=255)
                                                               cAAAGAACATCTGTATCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCCGCAAGCCGCCGGCGGCCGt                                                                                               <  6:238391/136‑1 (MQ=255)
                                                                    aaCATCTGTATCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCCGCAAGCCGCCGGCGGCCGTCAGTGCATCCGCCGT‑GTa                                                                            <  1:344950/149‑1 (MQ=255)
                                                                     tccgagctATCCGCGTCCGCAGCCACCGCTCCGCCGTGTCATTGTCCTGTGCCCCGCGGCAGGCCATGTGCAGTTCGAATCTGCGCACGGGTATCGGCAGGTCCTCGGGCCGCTAGCCGCCGGCGGCCGTCAGTGCATCCGCCGT‑GTa                                                                            <  5:267878/141‑1 (MQ=255)
                                                                      cATCTGTATCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGAGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCCGCAAGCCGCCGGCGGCCGTCAGTGCATCCGCCGT‑GTAGt                                                                          >  1:265910/1‑149 (MQ=255)
                                                                            tATCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCt                                                                                                                        >  1:338219/1‑98 (MQ=255)
                                                                            tATCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCt                                                                                                                        <  2:338219/98‑1 (MQ=255)
                                                                              tCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCCGCAAGCCGCCGGCGGCCGTCAGTGCATCCGCCGT‑GTAGTCCGGTAcc                                                                  >  4:337275/1‑149 (MQ=255)
                                                                              tCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCCGCAAGCCGCCGCCGGCCGTCAGTGCATCCCACGT‑GTAGTCCGGTAcc                                                                  >  4:342495/1‑149 (MQ=255)
                                                                                            ccccGCTCCGCCGGGTCACTGCCCGGGCCCCCGCGCCAGGCCCGCTGCAGGGCGAACCTCCGCACGGGCAGCGGCAGCTCCTCCGCCCCCACGCCGCCGGCGGCCGTCAGTGCACCCGCCGT‑GGAGTCCGGTACCGTAGCCACGATATc                                                    <  6:361521/147‑1 (MQ=255)
                                                                                             aCCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCCGCAAGCCGCCGGCGGCCGTCAGTGCATcc                                                                                     <  8:123443/116‑1 (MQ=255)
                                                                                             aCCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCCGCAAGCCGCCGGCGGCCGTCAGTGCATcc                                                                                     >  7:123443/1‑116 (MQ=255)
                                                                                               cGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCCGCAAGCCGCCGGCGGCCGTCAGTGCATCCGCCGT‑GTAGTCCGGTACCGTAGCCACGATATCGGt                                                 <  1:327245/149‑1 (MQ=255)
                                                                                                 cTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCCGCAAGCCGCCGGCGGCCGTCAGTGCATCCGCCGT‑GTAGTCCGGTACCGTAGCCACGATATCGGtgc                                               <  1:122668/149‑1 (MQ=255)
                                                                                                    cgccgGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCCGCAAGCCGCCGGCGGCCGTCAGTGCATCCGCCGT‑GTAGTCCGGTACCGTAGCCACGATATCGGtgcc                                              <  5:254147/147‑1 (MQ=255)
                                                                                                         ggTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCCGCAAGCCGCCGGCGGCCGTCAGTGCATCCGCCGT‑GTAGTCCGGTACCGTAGCCACGATATCGGTGCCTGCCAGc                                       <  7:337343/149‑1 (MQ=255)
                                                                                                              ttGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCCGCAAGCCGCCGGCGGCCGTCAGTGCATccgccg                                                                                 >  4:200986/1‑103 (MQ=255)
                                                                                                              ttGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCCGCAAGCCGCCGGCGGCCGTCAGTGCATccgccg                                                                                 <  3:200986/103‑1 (MQ=255)
                                                                                                                   cTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCCGCAAGCCGCCGGCGGCCGTCAGTGCATCCGCCGT‑GTAGTCCGGTACCGTAGCCACGATATCGGTGCCTGCCAGCAAGCTCCCCa                             <  6:263/149‑1 (MQ=255)
                                                                                                                    tgtgCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCCGCAAGCCGCCGGCGGCCGTCAGTGCATCCGCCGT‑GTAGTCCGGTACCGTAGCCACGATATCGGTGCCTGCCAGCAAGCTcccc                              >  6:122318/1‑147 (MQ=255)
                                                                                                                    tgtgCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCCGCAAGCCGCCGGCGGCCGTCAGTGCATCCGCCGT‑GTAGTCCGGTACCGTAGCCACGATATCGGTGCCTGCCAGCAAGCTcccc                              <  5:122318/147‑1 (MQ=255)
                                                                                                                         cccGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCCGCAAGCCGCCGGCGGCCGTCAGTGCATCCGCCGT‑GTAGTCCGGTACCGTAGCCACGATATCGGTGCCTGCCAGCAAGCTCCCCAGCCCGt                       >  2:20019/1‑149 (MQ=255)
                                                                                                                          ccGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCCGCAAGCCGCCGGCGGCCGTCAGTGCATCCGCCGT‑GTAGTCCGGTACCGTAGCCACGATATCGGTGCCTGCCAGCAAGCTCCCCAGCCCGtt                      <  1:20019/149‑1 (MQ=255)
                                                                                                                           cgcgCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCCGCAAGCCGCCGGCGGCCGTCTGGGGGGGGGGCGTGGGAATACGGTAACGTAGCCACGATATCGGTGCCTGCCAGCAAGCTCCCCAGCCCgat                      >  1:297967/1‑147 (MQ=255)
                                                                                                                           cgcgCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCCGCAAGCCGCCGGCGGCCGTCAGTGCATCCGCCGT‑GTAGTCCGGTACCGTAGCCACGATATCg                                                   <  3:278157/119‑1 (MQ=255)
                                                                                                                           cgcgCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCCGCAAGCCGCCGGCGGCCGTCAGTGCATCCGCCGT‑GTAGTCCGGTACCGTAGCCACGATATCg                                                   >  4:278157/1‑119 (MQ=255)
                                                                                                                           cgcgCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCCGCAAGCCGCCGGCGGCCGTCAGTGCATCCGCCGT‑GTAGTCCGGTACCGTAGCCACGATATCGGTGCCTGCCAGCAAGCTCCCCAGCCCGtt                      >  7:241138/1‑148 (MQ=255)
                                                                                                                                cAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCCGCAAGCCGCCGGCGGCCGTCAGTGCATCCGCCGT‑GTAGTCCGGTACCGTAGCCACGATATCGGTGCCTGCCAGCAAGCTCCCCAGCCCGTTGAACTg                >  2:22977/1‑149 (MQ=255)
                                                                                                                                              ttCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCCGCAAGCCGCCGGCGGCCGTCAGTGCATCCGCCGT‑GTAGTCCGGTACCGTAGCCACGATATCGGTGCCTGCCAGCAAGCTCCCCAGCCCGTTGAACTGCGGTACCGCCAGca  <  2:265910/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
ATTTGCTCACGAGGTCGTGCGATTTGATCTCAGTGGAAGCCTGTCACAAGCTATCGGGATCCCCAAAGAACATCTGTATCCGCGACCGCAGCCACCGCTCCGCCGGGTCATTGTCCTGTGCCCCGCGCCAGGCCATGTGCAGTTCGAAACTGCGCACGGGTATCGGCAGGTCCTCGGCCCGCAAGCCGCCGGCGGCCGTCAGTGCATCCGCCGT‑GTAGTCCGGTACCGTAGCCACGATATCGGTGCCTGCCAGCAAGCTCCCCAGCCCGTTGAACTGCGGTACCGCCAGCA  >  NC_002947/3224461‑3224751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑