Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,240,419	A→T	6.1%	I39F (ATC→TTC)	PP_2838 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,240,419	0	A	T	6.1%	198.7 / 2.9	66	I39F (ATC→TTC)	PP_2838	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (36/26);  new base T (2/2);  total (38/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.57e-01

CAGTGCCTGTTGCCGCAACCTGACCTCCGCCCGTGATCGCCCATTCCGTCGACGACCCGTTCTACTACCTGCATAACTTCCGCCAGGTCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCAAAGGCGAGTTGTTTCGCAGCGATCGGCTCGACTACGCCGAAATCGGCGATACTGCGCTGGCGCTGCAGCCGTTGCTGGCCCTC  >  NC_002947/3240273‑3240565                                                                                                                                                   |                                                                                                                                                   cAGTGCCTGTTGCCGCAACCTGACCTCCGCCCGTGATCGCCCATTCCGTCGACGACCCGTTCTACTACCTGCATAACTTCCGCCAGGTCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATc                                                                                                                                                  >  7:213807/1‑149 (MQ=255)  aGTGCCTGTTGCCGCAACCTGACCTCCGCCCGTGATCGCCCATTCCGTCGACGACCCGTTCTACTACCTGCATAACTTCCGCCAGGTCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATcc                                                                                                                                                 <  5:34279/149‑1 (MQ=255)    tGCCTGTTGCCGCAACCTGACCTCCGCCCGTGATCGCCCATTCCGTCGACGACCCGTTCTACTACCTGCATAACTTCCGCCAGGTCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGt                                                                                                                                               <  6:224756/149‑1 (MQ=255)          ttGCCGCACCAGGACCTCAGCCAGAGATCGCCCATTCCGTCGACGACCCGTTCTACTACCTGCATAACTTCCGCCAGGTCGTGCTTTGGGTCGAACAGCGCTACAAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCtt                                                                                                                                          <  4:271882/148‑1 (MQ=255)             ccGCAACCTGACCTCCGCCCGTGATCGCCCATTCCGTCGACGACCCGTTCTACTACCTGCATAACTTCCGCCAGGTCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAg                                                                                                                                       <  8:213807/148‑1 (MQ=255)              cGCAACCTGACCTCCGCCCGTGATCGCCCATTCCGTCGACGACCCGTTCTACTACCTGCATAACTTCCGCCAGGTCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTc                                                                                                                                     <  3:174192/149‑1 (MQ=255)                cAACCTGACCTCCGCCCGTGATCGCCCATTCCGTCGACGACCCGTTCTACTACCTGCATAACTTCCGCCAGGTCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCa                                                                                                                                        <  1:40762/144‑1 (MQ=255)                cAACCTGACCTCCGCCCGTGATCGCCCATTCCGTCGACGACCCGTTCTACTACCTGCATAACTTCCGCCAGGTCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCa                                                                                                                                        >  2:40762/1‑144 (MQ=255)                          tCCGCCCGTGATCGCCCATTCCGTCGACGACCCGTTCTACTACCTGCATAACTTCCGCCAGGTCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCCACTGCAAgcgc                                                                                                                         >  3:286981/1‑149 (MQ=255)                          tCCGCCCGTGATCGCCCATTCCGTCGACGACCCGTTCTACTACCTGCATAACTTCCGCCAGGTCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAgcgc                                                                                                                         >  7:77340/1‑149 (MQ=255)                            cGCCCGTGATCGCCCATTCCGTCGACGACCCGTTCTACTACCTGCATAACTTCCGCCAGGTCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCa                                                                                                                       >  2:73205/1‑149 (MQ=255)                                     tCGCCCATTCCGTCGACGACCCGTTCTACTACCTGCATAACTTCCGCCAGGTCGTGCTGTGGCTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGcc                                                                                                              >  5:67529/1‑149 (MQ=255)                                       gCCCATTCCGTCGACGACCCGTTCTACTACCTGCATAACTTCCGCCAGGTCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCtt                                                                                                            <  1:73205/149‑1 (MQ=255)                                       gCCCATTCCGTCGACGACCCGTTCTACTACCTGCATAACTTCCGCCAGGTCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATAACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCtt                                                                                                            >  8:168477/1‑149 (MQ=255)                                       gCCCATTCCGTCGACGACCCGTTCTAATACCTGCATAACTTCCGCCAGGTCATGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCtt                                                                                                            >  1:193323/1‑149 (MQ=255)                                         ccATTCCGTCGACGACCCGTTCTACTACCTGCATAACTTCCGCCAGGTCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGa                                                                                                          <  2:53021/149‑1 (MQ=255)                                         ccATTCCGTCGACGACCCGTTCTACTACCTGCATAACTTCCGCCAGGTCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGATGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGa                                                                                                          >  3:277718/1‑149 (MQ=255)                                             tCCGTCGACGACCGGTTCTACTACCTGCATAACTTGCGGCTGGTCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCGTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATgg                                                                                                       <  7:204621/148‑1 (MQ=255)                                             accGTCGACGACCCGTTCTACTACCTCCATAACTTCCGCCAGGTCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCTTGCCTTCAGTCAACTGCAAGCCCCAGCCCAGGCCATGATgg                                                                                                       >  8:204621/2‑148 (MQ=255)                                                     cgacCCGTTCTACTACCTGCATAACTTCCGCCAGGTCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATgg                                                                                              <  8:77340/149‑1 (MQ=255)                                                     cgacCCGTTCTACTACCTGCATAACTTCCGCCAGGTCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTAGATGGTGCGCATgg                                                                                              <  3:1441/149‑1 (MQ=255)                                                         ccGTTCTACTACCTGCATAACTTCCGCCAGGTCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCtt                                                                                                                                          <  5:44279/101‑1 (MQ=255)                                                         ccGTTCTACTACCTGCATAACTTCCGCCAGGTCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCtt                                                                                                                                          >  6:44279/1‑101 (MQ=255)                                                              ctactaCCTGCATAACTTCCGCCAGGTCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATgcgc                                                                                      >  6:264050/1‑148 (MQ=255)                                                                actacCTGCATAACTTCCGCCAGGTCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCa                                                                                     >  3:301941/1‑147 (MQ=255)                                                                 ctacCTGCATAACTTCCGCCAGGTCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCAAAg                                                                                  >  7:92327/1‑149 (MQ=255)                                                                    ccTGCATAACTTCCGCCAGGTCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCAAAGGc                                                                                >  8:196567/1‑148 (MQ=255)                                                                    ccTGCATAACTTCCGCCAGGTCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCAAAGGc                                                                                >  7:289826/1‑148 (MQ=255)                                                                     cTGCATAACTTCCGCCAGGTCATGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCAAAGGCGa                                                                              <  2:193323/149‑1 (MQ=255)                                                                          tAACTTCCGCCAGGTCGTGCTGTGGGTCCAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTgc                                                                                                    <  8:162081/122‑1 (MQ=255)                                                                             cTTCCGCCAGGTCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCaaa                                                                                   <  4:65978/136‑1 (MQ=255)                                                                             cTTCCGCCAGGTCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCaaa                                                                                   >  3:65978/1‑136 (MQ=255)                                                                             cTTCCGCCAGGTCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCAAAGGCGAGTTGTTTc                                                                      <  8:289826/149‑1 (MQ=255)                                                                              ttCCGCCAGGTCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCAAAGGCGAGTTGTTTCg                                                                     >  1:308060/1‑149 (MQ=255)                                                                              ttCCGCCAGGTCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCAAAGGCGAGTTGTTTCg                                                                     >  5:218825/1‑149 (MQ=255)                                                                              ttCCGCCAGGTCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCAAAGGCGAGTTGTTTCg                                                                     >  3:197647/1‑149 (MQ=255)                                                                              ttCCGCCAGGTCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCAAAGGCGAGTTGTTTCg                                                                     >  3:232962/1‑149 (MQ=255)                                                                              ttCCGCCAGGACGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGACTTCAGTCAACTGCAAGCGCCAGCCCAGGCCATGATGGTGCGCATGGTGATGCGAAAAGGCGAGTTGATTCg                                                                     >  4:3487/1‑149 (MQ=255)                                                                                 cGCCAGGTCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCAAAGGCGAGTTGTTTCGCAg                                                                  >  1:355078/1‑149 (MQ=255)                                                                                  gCCAGGTCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCAAAGGCGAGTTGTTTCGCAGc                                                                 <  4:125522/149‑1 (MQ=255)                                                                                        tCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCAAAGGCGAGTTGTTTCGCAGCGATCgg                                                           <  4:301941/149‑1 (MQ=255)                                                                                           tGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCAAAGGCGAGTTGTTTCGCAGCGATCGGCTc                                                        <  4:197647/149‑1 (MQ=255)                                                                                            gCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCAAAGGCGAGTTGTTTCGCAGCGATCGGCTc                                                        >  8:46941/1‑148 (MQ=255)                                                                                                 gggTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCAAAGGCGAGTTGTTTCGCAGCGATCGGCTCGACTAc                                                  >  8:29384/1‑149 (MQ=255)                                                                                                     cGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCAAAGGCGAGTTGTTTCGCAGCGATCGGCTCGACTACGcc                                               >  6:191741/1‑148 (MQ=255)                                                                                                       aaCAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTgcgc                                                                                                  >  6:265787/1‑95 (MQ=255)                                                                                                            cgcTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCAAAGGCGAGTTGTTTCGCAGCGATCGGCTCGACTACGCCGAAATCgg                                       >  3:260060/1‑149 (MQ=255)                                                                                                              cTACCAGGACCTTCTTGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCAAAGTCGAGTTGTTTCGCAGCTATCGGCTCGACTACGCCGAAATCGGCg                                     <  6:156065/149‑1 (MQ=255)                                                                                                              cTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCAAAGGCGAGTTGTTTCGCAGCGATCGGCTCGACTACGCCGAAATCGGCg                                     <  8:13891/149‑1 (MQ=255)                                                                                                                aCCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCAAAGGCGAGTTGTTTCGCAGCGATCGGCTCGACTACGCCGAAATCGGCGa                                    >  2:87556/1‑148 (MQ=255)                                                                                                                  cAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCAAAGGCGAGTTGTTTCGCAGCGATCGGCTCGACTACGCCGAAATCGGCGATAc                                 <  7:29384/149‑1 (MQ=255)                                                                                                                   aGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCAAAGGCGAGTTGTTTCGCAGCGATCGGCTCGACTACGCCGAAATCGGCGATACt                                >  4:153359/1‑149 (MQ=255)                                                                                                                      aCCTGCTGGATAACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCACAAAGGCGAGTTGTTTCGCAGCGATCGGCTCGACTACGCCGAAATCGGCGATACTgcg                             <  7:168477/149‑1 (MQ=255)                                                                                                                        ctgctgGATGACCAGGAACTGGCCTTCTTCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCAAAGGCGAGTTGTTTCGCAGCGATCGGCTCGACTACGCCGAAATCGGCGATACTgcgct                           >  7:192043/1‑149 (MQ=255)                                                                                                                        ctgctgGATGACCAGGAACTGGCCTTCTTCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCAAAGGCGAGTTGTTTCGCAGCGATCGGCTCGACTACGCCGAAATCGGCGATACTgcgct                           >  7:186844/1‑149 (MQ=255)                                                                                                                              gATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCAAAGGCGAGTTGTTTCGCAGCGATCGGCTCGACTACGCCGAAATCGGCGATCCGgcgctgagat                      >  4:220498/1‑144 (MQ=255)                                                                                                                                   ccAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCAAAGGCGAGTTGTTTCGCAGCGATCGGCTCGACTACGCCGAAATCGGCGATACTGCGCTGGCGCTGCAGc                >  8:187090/1‑149 (MQ=255)                                                                                                                                    cAGGAACTGGCCTTCTTCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCAAAGGCGAGTTGTTTCGCAGCGATCGGCTCGACTACGCCGAAATCGGCGATACTGCGCTGGCGCTGCAGcc               <  8:186844/149‑1 (MQ=255)                                                                                                                                    cAGGAACTGGCCTTCTTCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCAAAGGCGAGTTGTTTCGCAGCGATCGGCTCGACTACGCCGAAATCGGCGATACTGCGCTGGCGCTGCAGcc               <  8:192043/149‑1 (MQ=255)                                                                                                                                    cAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCAAAGGCGAGTTGTTTCGCAGCGATCGGCTCGACTACGCCGAAATCGGCGATACTGCGCTGGCGCTGCAGcc               >  7:241275/1‑149 (MQ=255)                                                                                                                                         aCTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCAAAGGCGAGTTGTTTCGCAGCGATCGGCTCGACTACGCCGAAATCGGCGATACTGCGCTGGCGCTGCAGCCGTTGc          <  8:241275/149‑1 (MQ=255)                                                                                                                                             gCCTTCATCGGTGCCTTCAGTCAACTGCATGCGCCAGCCGAGGCCTTGATGGTGCGCATGGTTATGCGAAAAGGCGAGTTGTTTGGCAGCGATCGGCTCGACTACGCCGAAATCGGCGATACTGCGCTGGCGCTGCAGCCGTTGCTg        <  6:67529/147‑1 (MQ=255)                                                                                                                                             gCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCAAAGGCGAGTTGTTTCGCAGCGATCGGCTCGACTACGCCGAAATCGGCGATACTGCGCTGGCGCTGCAGCCGTTGCTGGc      <  8:92327/149‑1 (MQ=255)                                                                                                                                              ccTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCAAAGGCGAGTTGTTTCGCAGCGATCGGCTCGACTACGCCGAAATCGGCGATACTGCGCTGGCGCTGCAGCCGTTGCTGGcc     >  2:44559/1‑149 (MQ=255)                                                                                                                                               cTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCAAAGGCGAGTTGTTTCGCAGCGATCGGCTCGACTACGCCGAAATCGGCGATACTGCGCTGGCGCTGCAGCCGTTGCTGGccc    >  5:44545/1‑149 (MQ=255)                                                                                                                                                 tCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCAAAGGCGAGTTGTTTCGCAGCGATCGGCTCGACTACGCCGAAATCGGCGATACTGCGCTGGCGCTGCAGCCGTTGCTGGCCCTc  >  2:23145/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CAGTGCCTGTTGCCGCAACCTGACCTCCGCCCGTGATCGCCCATTCCGTCGACGACCCGTTCTACTACCTGCATAACTTCCGCCAGGTCGTGCTGTGGGTCGAACAGCGCTACCAGGACCTGCTGGATGACCAGGAACTGGCCTTCATCCGTGCCTTCAGTCAACTGCAAGCGCCAGCCCAGGCCTTGATGGTGCGCATGGTGATGCGCAAAGGCGAGTTGTTTCGCAGCGATCGGCTCGACTACGCCGAAATCGGCGATACTGCGCTGGCGCTGCAGCCGTTGCTGGCCCTC  >  NC_002947/3240273‑3240565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑