Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 288,319 A→T 7.0% intergenic (‑212/‑263) tauA ← / → oprE taurine ABC transporter substrate‑binding protein/outer‑membrane porin E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947288,3190AT 7.0% 169.1 / 2.7 57intergenic (‑212/‑263)tauA/oprEtaurine ABC transporter substrate‑binding protein/outer‑membrane porin E
Reads supporting (aligned to +/- strand):  ref base A (31/22);  new base T (2/2);  total (33/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

AAATAAATAACTTTTTTGCATTAGCTTAGGGGCCAAACGCTTTAAAGCCATGACCCGTGACGAAGCCAGAGGTGCTTGCAAGACCCTATAGAACGATTTGGCATTAGGTTATGAGCAAACGTTTGCAAATAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCATAAAACAGTCATTTTGGATTTATAGGATTGTCATTATCCTGTAGCGCAGGTGGCGTCTTAGACCAAAGTCGCGAAACGTTT  >  NC_002947/288173‑288467
                                                                                                                                                  |                                                                                                                                                    
aaataaatAACTTTTTTGCATTAGCTTAGGGGCCAAACGCTTTAAAGCCATGACCCGTGACGAAGCCAGAGGTGCTTGCAAGACCCTATAGAACGATTTGGCATTAGGTTATGAGCAAACGTTTGCAAATAACCAAAAGGTATTAAAAt                                                                                                                                                    <  7:174850/149‑1 (MQ=255)
  ataaataaCTTTTTTGCATTAGCTTAGGGGCCAAACGCTTTAATGCCATGCCCCGTGACGAAGCCAGAGGTGCTTGCAAGACCGTAGAGATCGATTTGGCATTAGGTGATGAGCAAGCGTTTGCAATTAACCAAAAGGTATTTAAAgct                                                                                                                                                  >  4:324016/1‑149 (MQ=255)
   taaataaCTTTTTTGCATTAGCTTAGGGGCCAAACGCTTTAAAGCCATGACCCGTGACGAAGCCAGAGGTGCTTGCAAGACCCTATAGAACGATTTGGCATTAGGTTATGAGCGAACGTTTGCAAATAACCAAAAGGTATTAAAAtctt                                                                                                                                                 >  6:345117/1‑149 (MQ=255)
      ataaCTTTTTTGCATTAGCTTAGGGGCCAAACGCTTTAAAGCCATGACCCGTGACGAAGCCAGAGGTGCTTGCAAGACCCTATAGAACGATTTGGCATTAGGTTATGAGCAAACGTTTGCAAATAACCAAAAGGTATTAAAATCTtctc                                                                                                                                              <  2:206952/149‑1 (MQ=255)
        aaCTTTTTTGCATTAGCTTAGGGGCCAAACGCTTTAAAGCCATGACCCGTGACGAAGCCAGAGGTGCTTGCAAGACCCTATAGAACGATTTGGCATTAGGTTATGAGCAAACGTTTGCAAATAACCAAAAGGTATTAAAATCTTCTCTg                                                                                                                                            >  4:359780/1‑149 (MQ=255)
           ttttttGCATTAGCTTAGGGGCCAAACGCTTTAAAGCCATGACCCGTGACGAAGCCAGAGGTGCTTGCAAGACCCTATAGAACGATTTGGCATTAGGTTATGAGCAAACGTTTGCAAATAACCAAAAGGTATTAAAATCTTCTCTGCTa                                                                                                                                         >  5:134971/1‑149 (MQ=255)
                      aGCTTAGGGGCCAAACGCTTTAAAGCCATGACCCGTGACGAAGCCAGAGGTGCTTGCAAGACCCTATAGAACGATTTGGCATTAGGTTATGAGCAAACGTTTGCAAATAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATgg                                                                                                                               <  6:134971/148‑1 (MQ=255)
                                 cAAACGCTTTAAAGCCATGACCCGTGACGAAGCCAGAGGTGCTTGCAAGACCCTATAGAACGATTTGGCATTAGGTTATGAGCAAACGTTTGCAAATAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGttt                                                                                                                   <  2:301538/149‑1 (MQ=255)
                                  aaaCGCTTTAAAGCCATGACCCGTGACGAAGCCAGAGGTGCTTGCAAGACCCTATAGAACGATTTGGCATTAGGTTATGAGCAAACGTTTGCAAATAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTc                                                                                                                  >  4:229/1‑149 (MQ=255)
                                   aaCGCTTTAAAGCCATGACCCGTGACGAAGCCAGAGGTGCTTGCAAGACCCTATAGAACGATTTGGCATTAGGTTATGAGCAAACGTTTGCAAATAACCAAAAGGTAATAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTc                                                                                                                  >  5:312311/1‑148 (MQ=255)
                                     cGCTTTAAAGCCATGACCCGTGACGAAGCCAGAGGTGCTTGCAAGACCCTATAGAACGATTTGGCATTAGGTTATGAGCAAACGTTTGCAAATAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTaa                                                                                                               <  3:229/149‑1 (MQ=255)
                                      gCTTTAAAGCCATGACCCGTGACGAAGCCAGAGGTGCTTGCAAGACCCTATAGAACGATTTGGCATTAGGTTATGAGCAAACGTTTGCAAATAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAat                                                                                                              <  2:137194/149‑1 (MQ=255)
                                               ccATGACCCGTGACGAAGCCAGAGGTGCTTGCAGGACCCTATAGAACGATTTGGCATTAGGTTATGAGCAAACGTTTGCAAATAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCt                                                                                                      >  5:339933/1‑148 (MQ=255)
                                                 aTGACCCGTGACGAAGCCAGAGGTGCTTGCAAGACCCTATAGAACGATTTGGCATTAGGTTATGAGCAAACGTTTGCAAATAACCAAAAGGTATTAAAATCTTCTCTg                                                                                                                                            >  4:50940/1‑108 (MQ=255)
                                                      ccGTGACGAAGCCAGAGGTGCTTGCAAGACCCTATAGAACGATTTGGCATTAGGTTATGAGCAAACGTTTGCAAATAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTc                                                                                                                  >  6:291507/1‑129 (MQ=255)
                                                      ccGTGACGAAGCCAGAGGTGCTTGCAAGACCCTATAGAACGATTTGGCATTAGGTTATGAGCAAACGTTTGCAAATAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTc                                                                                                                  <  5:291507/129‑1 (MQ=255)
                                                      ccGTGACGAAGCCAGAGGTGCTTGCAAGACCCTATAGAACGATTTGGCATTAGGTTATGAGCAAACGTTTGCAAATAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTc                                                                                              <  4:147178/149‑1 (MQ=255)
                                                             gAAGCCAGAGGTGCTTGCAAGACCCTATAGAACGATTTGGCATTAGGTTATGAGCAAACGTTTGCAAATAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAg                                                                                                                       <  5:52465/117‑1 (MQ=255)
                                                             gAAGCCAGAGGTGCTTGCAAGACCCTATAGAACGATTTGGCATTAGGTTATGAGCAAACGTTTGCAAATAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAg                                                                                                                       >  6:52465/1‑117 (MQ=255)
                                                                       gTGCTTGCAAGACCCTATAGAACGATTTGGCATTAGGTTATGAGCAAACGTTTGCAAATAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGttt                                                                                                                   >  4:204232/1‑111 (MQ=255)
                                                                                      tataGAACGATTTGGCATTAGGTTATGAGCAAACGTTTGCAAATAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCATAAAACAGTCATTTTGGAtt                                                               >  3:221670/1‑148 (MQ=255)
                                                                                           aaCGATTTGGCATTAGGTTATGAGCAAACGTTTGCAAATAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTa                                                                                           >  2:45380/1‑115 (MQ=255)
                                                                                           aaCGATTTGGCATTAGGTTATGAGCAAACGTTTGCAAATAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTa                                                                                           <  1:45380/115‑1 (MQ=255)
                                                                                                      aTTAGGTTATGAGCAAACGTTTGCAAATAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCt                                                                                             <  5:317907/102‑1 (MQ=255)
                                                                                                      aTTAGGTTATGAGCAAACGTTTGCAAATAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCt                                                                                             >  6:317907/1‑102 (MQ=255)
                                                                                                       ttAGGTTATGAGCAAACGTTTGCAAATAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCAt                                                                                  <  8:24398/112‑1 (MQ=255)
                                                                                                       ttAGGTTATGAGCAAACGTTTGCAAATAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCAt                                                                                  >  7:24398/1‑112 (MQ=255)
                                                                                                           gTTATGAGCAAACGTTTGCAAATAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCATAAAACAGTCATTTTGGATTTATAGGATTGTCATTATCCTg                                          >  3:325048/1‑148 (MQ=255)
                                                                                                              aTGAGCAAACGTTTGCAAATAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCATAAAACAGTCATTTTGGATTTATAGGATTGTCATTATCCTGTAgc                                      >  3:11578/1‑149 (MQ=255)
                                                                                                                   cAAACGTTTGCAAATAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCATAAAACAGTCATTTTGGATTTATAGGATTGTCATTATCCTGTAGCGCAgg                                 >  1:182955/1‑149 (MQ=255)
                                                                                                                    aaaCGTTTGCAAATAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGGTGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCATAAAACAGTCATTTTGGATTTATAGGATTGTCATTATCCTGTAGCGCAGGt                                >  1:204866/1‑149 (MQ=255)
                                                                                                                    aaaCGTTTGCAAATAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCATAAAACAGTCATTTTGGa                                                                 >  2:350660/1‑116 (MQ=255)
                                                                                                                    aaaCGTTTGCAAATAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCATAAAACAGTCATTTTGGATTTATAGGATTGTCATTATCCTGTAGCGCAGGt                                >  8:17625/1‑149 (MQ=255)
                                                                                                                    aaaCGTTTGCAAATAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCATAAAACAGTCATTTTGGATTTATAGGATTGTCATTATCCTGTAGCGCAGGt                                >  6:62643/1‑149 (MQ=255)
                                                                                                                       cGTTTTCAAATAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCATAAAACAGTCATTTTGGATTTATAGGATTGTCATTATCCTGTAGCGCAGGTGGc                             <  6:339933/149‑1 (MQ=255)
                                                                                                                         tttGCAAATAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCATAAAACAGTCATTTTGGATTTATAGGATTGTCATTATCCTGTAGCGCAGGTGGCGt                           <  3:359780/149‑1 (MQ=255)
                                                                                                                            gCAAATAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCATAAAACAGTCATTTTGGATTTATAGGATTGTCATTATCCTGTa                                        <  5:204968/133‑1 (MQ=255)
                                                                                                                            gCAAATAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCATAAAACAGTCATTTTGGATTTATAGGATTGTCATTATCCTGTa                                        >  6:204968/1‑133 (MQ=255)
                                                                                                                             cgatctaCCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCATAAAACAGTCATTTTGGATTTATAGGATTGTCATTATCCTGTAGCGCAGGTGGCGTCTTa                       <  5:172311/143‑1 (MQ=255)
                                                                                                                              aaaTAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAATGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCATAAAACAGTCATTTTGGATTTATAGGATTGTCATTATCCTGTAGCGCAGGTGGCGTCt                         >  6:6112/1‑146 (MQ=255)
                                                                                                                              aaaTAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCATAAAACAGTCATTTTGGATTTATAGGATTGTCATTATCCTGTAGCGCAGGTGGCGTCt                         >  7:116855/1‑146 (MQ=255)
                                                                                                                              aaaTAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCATAAAACAGTCATTTTGGATTTATAGGATTGTCATTATCCTGTAGCGCAGGTGGCGTCt                         >  2:93583/1‑146 (MQ=255)
                                                                                                                              aaaTAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCATAAAACAGTCATTTTGGATTTATAGGATTGTCATTATCCTGTAGCGCAGGTGGCGTCTTa                       <  4:221670/148‑1 (MQ=255)
                                                                                                                               aaTAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGGTGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCATAAAACAGTCATTTTGGATTTATAGGATTGTCATTATCCTGTAGCGCAGGTGGCGTCTTAGa                     <  2:204866/149‑1 (MQ=255)
                                                                                                                               aaTAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCATAAAACAGTCATTTTGGATTTATAGGATTGTCATTATCCTGTAGCGCAGGTGGCGTCTTAGa                     <  7:17625/149‑1 (MQ=255)
                                                                                                                                    ccAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCATAAAACAGTCATTTTGGATTTATAGGATTGTCATTATCCTGTAGCGCAGGTGGCGTCTTa                       >  6:172311/1‑142 (MQ=255)
                                                                                                                                     cAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCATAAAACAGTCATTTTGGATTTATAGGATTGTCa                                                  <  4:97176/114‑1 (MQ=255)
                                                                                                                                     cAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCATAAAACAGTCATTTTGGATTTATAGGATTGTCa                                                  >  3:97176/1‑114 (MQ=255)
                                                                                                                                       aaaGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCATAAAACAGTCATTTTGGATTTATAGGATTGTCATTATCCTGTAGCGCAGGTGGCGTCTTAGACCAAAGTc             >  2:81359/1‑149 (MQ=255)
                                                                                                                                       aaaGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCATAAAACAGTCATTTTGGATTTATAGGATTGTCATTATCCTGTAGCGCAGGTGGCGTCTTAGACCAAAGTc             >  7:233201/1‑149 (MQ=255)
                                                                                                                                       aaaGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCATAAAACAGTCATTTTGGATTTATAGGATTGTCATTATCCTGTAGCGCAGGTGGCGTCTTAGACCAAAGTc             <  4:325048/149‑1 (MQ=255)
                                                                                                                                         aGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCATAAAACAGTCATTTTGGATTTATAGGATTGTCATTATCCTGTAGCGCAGGTGGCGTCTTAGACCAAAGTcgc           >  5:302383/1‑149 (MQ=255)
                                                                                                                                            tATTAATATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCATAAAACAGTCATTTTGGATTTATAGGATTGTCATTATCCTGTAGCGCAGGTGGCGTCTTAGACCaaa                >  6:226985/1‑141 (MQ=255)
                                                                                                                                            tATTAATATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCATAAAACAGTCATTTTGGATTTATAGGATTGTCATTATCCTGTAGCGCAGGTGGCGTCTTAGACCaaa                >  6:221795/1‑141 (MQ=255)
                                                                                                                                            tATTAATATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCATAAAACAGTCATTTTGGATTTATAGGATTGTCATTATCCTGTAGCGCAGGTGGCGTCTTAGACCaaa                <  5:226985/141‑1 (MQ=255)
                                                                                                                                            tATTAATATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCATAAAACAGTCATTTTGGATTTATAGGATTGTCATTATCCTGTAGCGCAGGTGGCGTCTTAGACCaaa                <  5:221795/141‑1 (MQ=255)
                                                                                                                                              ttAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCATAAAACAGTCATTTTGGATTTATAGGATTGTCATTATCCTGTAGCGCAGGTGGCGTCTTAGACCAAAGTCGCGAAAc      <  4:11578/149‑1 (MQ=255)
                                                                                                                                                aaaaTCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCATAAAACAGTCATTTTGGATTTATAGGATTGTCATTATCCTGTAGCGCAGGTGGCGTCTTAGACCAAAGTCGCGAAACGt    >  2:216131/1‑149 (MQ=255)
                                                                                                                                                  aaTCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCATAAAACAGTCATTTTGGATTTATAGGATTGTCATTATCCTGTAGCGCAGGTGGCGTCTTAGACCAAAGTCGCGAAACGttt  <  2:182955/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
AAATAAATAACTTTTTTGCATTAGCTTAGGGGCCAAACGCTTTAAAGCCATGACCCGTGACGAAGCCAGAGGTGCTTGCAAGACCCTATAGAACGATTTGGCATTAGGTTATGAGCAAACGTTTGCAAATAACCAAAAGGTATTAAAATCTTCTCTGCTACAACGGATGGCAGTAAAGGTTTCTAATATATTCCTTAAAAGTCTTACAAATTCATAAAACAGTCATTTTGGATTTATAGGATTGTCATTATCCTGTAGCGCAGGTGGCGTCTTAGACCAAAGTCGCGAAACGTTT  >  NC_002947/288173‑288467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑