Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,300,600 C→T 9.8% E103K (GAG→AAG) ‡ lpxH ← UDP‑2,3‑diacylglucosamine hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,300,6000CT 9.8% 166.0 / 6.9 62E103K (GAG→AAG) ‡lpxHUDP‑2,3‑diacylglucosamine hydrolase
Reads supporting (aligned to +/- strand):  ref base C (29/26);  new base T (3/3);  total (33/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.57e-01

GCCAGCTTGTAGCGGGCCGACAGCGGCAGGTGGCGTAGGATCCACAAGCTCAGCGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCAGCCGGCGCATGGCCTGGCAGATCGACTG  >  NC_002947/3300452‑3300741
                                                                                                                                                    |                                                                                                                                             
gCCAGCTTGTAGCGGGCCGACAGCGGCAGGTGGCGTAGGATCCACAAGCTCAGCGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTc                                                                                                                                               >  7:205745/1‑149 (MQ=255)
   aGCTTGTAGCGGGCCGACAGCGGCAGGTGGCGTAGGATCCACAAGCTCAGCGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGAt                                                                                                                                            <  2:319468/149‑1 (MQ=255)
   aGCTTGTAGCGGGCCGACAGCGGCAGGTGGCGTAGGATCCACAAGCTCAGCGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGAt                                                                                                                                            <  7:91524/149‑1 (MQ=255)
    gCTTGTAGCGGGCCGACAGCGGCAGGTGGCGTAGGATCCACAAGCTCAGCGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGAt                                                                                                                                            >  7:268102/1‑148 (MQ=255)
             gggCCGACAGCGGCAGGTGGCGTAGGATCCACAAGCTCAGCGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTgggg                                                                                                                                  <  4:165558/149‑1 (MQ=255)
               gCCGACAGCGGCAGGTGGCGTAGGATCCACAAGCTCAGCGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCGTGATCGAGCTgggtgg                                                                                                                                <  1:89787/149‑4 (MQ=255)
                   aCAGCGGCAGGTGGCGTAGGATCCACAAGCTCAGCGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGtt                                                                                                                             >  6:113159/1‑148 (MQ=255)
                    cAGCGGCAGGTGGCGTAGGATCCACAAGCTCAGCGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCATCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCa                                                                                                                           <  1:7918/149‑1 (MQ=255)
                       cGGCAGGTGGCGTAGGATCCACAAGCTCAGCGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACa                                                                                                                        <  2:85426/149‑1 (MQ=255)
                            ggTGGCGTAGGATCCACAAGCTCAGCGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTg                                                                                                                   <  7:209525/149‑1 (MQ=255)
                                gCGTAGGATCCACAAGCTCAGCGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAg                                                                                                                <  8:268102/148‑1 (MQ=255)
                                  gTAGGATCCACAAGCTCAGCGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGccc                                                                                                             >  4:273066/1‑149 (MQ=255)
                                          cacaAGCTCAGCGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGc                                                                                                               <  4:308679/139‑1 (MQ=255)
                                          cacaAGCTCAGCGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGc                                                                                                               >  3:308679/1‑139 (MQ=255)
                                          cacaAGCTCAGCGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGCGCCCTGGTGCACAGCGTGTCCCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCttt                                                                                                     <  1:116813/149‑1 (MQ=255)
                                                cTCAGCGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCaaaa                                                                                               <  3:273066/149‑1 (MQ=255)
                                                 tCAGCGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACCGCGTGCAGCCCGCAGCTTTGCAAAAg                                                                                              >  7:295534/1‑149 (MQ=255)
                                                   aGCGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCGACAGCGTGCAGCCCGCAGCTTTGCAAAAGGc                                                                                            >  6:350026/1‑149 (MQ=255)
                                                     cGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCa                                                                                                          >  3:25885/1‑133 (MQ=255)
                                                     cGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCa                                                                                                          <  4:25885/133‑1 (MQ=255)
                                                            cGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAAt                                                                                   >  2:287696/1‑149 (MQ=255)
                                                             gTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCGACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATc                                                                                  <  5:350026/149‑1 (MQ=255)
                                                             gTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAAt                                                                                   >  2:300165/1‑148 (MQ=255)
                                                                  aGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGaa                                                                             <  8:205745/149‑1 (MQ=255)
                                                                      ggcgCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCa                                                                         >  4:87154/1‑149 (MQ=255)
                                                                         gcATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACgg                                                                      >  2:118865/1‑149 (MQ=255)
                                                                                cATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTg                                                                   <  1:287696/145‑1 (MQ=255)
                                                                                cATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGc                                                                  <  1:300165/146‑1 (MQ=255)
                                                                                 aTGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGtt                                                                    <  7:52648/143‑1 (MQ=255)
                                                                                 aTGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATg                                                              >  1:134791/1‑149 (MQ=255)
                                                                                  tGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGc                                                             <  1:118865/149‑1 (MQ=255)
                                                                                   gTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCa                                                            >  2:101950/1‑149 (MQ=255)
                                                                                        ccAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAgg                                                       >  8:22072/1‑149 (MQ=255)
                                                                                         cAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAgg                                                       >  2:170107/1‑148 (MQ=255)
                                                                                               gcgGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCaa                                                                                                 >  3:343620/1‑100 (MQ=255)
                                                                                               gcgGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCaa                                                                                                 <  4:343620/100‑1 (MQ=255)
                                                                                                    tGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAAt                                                                                   <  1:88281/109‑1 (MQ=255)
                                                                                                    tGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAAt                                                                                   >  2:88281/1‑109 (MQ=255)
                                                                                                       acaGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCa                                        <  7:22072/149‑1 (MQ=255)
                                                                                                       acaGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCa                                        >  5:20483/1‑149 (MQ=255)
                                                                                                           ggTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGt                                    >  4:335526/1‑149 (MQ=255)
                                                                                                                 gCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCTACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCtgt                              <  8:295534/149‑3 (MQ=255)
                                                                                                                 gCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCtgg                              >  1:128127/1‑147 (MQ=255)
                                                                                                                   cATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCAGc                            >  6:289573/1‑149 (MQ=255)
                                                                                                                       cATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTATATGGCCGTGCCACTGTCGCTCa                              <  7:227855/143‑1 (MQ=255)
                                                                                                                       cATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCa                              >  8:227855/1‑143 (MQ=255)
                                                                                                                        aTCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGACATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCAGCCGgcg                       >  3:228331/1‑149 (MQ=255)
                                                                                                                        aTCAGCAGCACCTCATCACCACCCAGCAACATCACCCAGTGCTCCTTCAACATCGTCCAGCCCCCAGATAAGCAAAAGGCCTGGCCAATCATGAACTCACCGTTGCCATGAATCAGGTAAATCGACGTCCCACTCACGCTCAGccgccg                       >  6:303401/1‑149 (MQ=255)
                                                                                                                            gcagcaCCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTgga                                                                                       >  8:235221/1‑80 (MQ=255)
                                                                                                                            gcagcaCCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCAGCCGGCGCATg                   <  5:113159/149‑1 (MQ=255)
                                                                                                                             cagcaCCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCAGCCGgc                        >  7:188969/1‑143 (MQ=255)
                                                                                                                             cagcaCCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCAGCCGgc                        <  8:188969/143‑1 (MQ=255)
                                                                                                                              agcaCCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCAGCCGGCGCATGGc                 >  3:84480/1‑149 (MQ=255)
                                                                                                                                 aCCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCAGCCGGCGCATGGCCTg              >  2:257210/1‑149 (MQ=255)
                                                                                                                                  ccTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCAGCCGGCGCATGGCCTgg             >  4:303182/1‑149 (MQ=255)
                                                                                                                                   cTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCAGCCGGCGCATGGCCTGGc            <  1:101950/149‑1 (MQ=255)
                                                                                                                                         accaccCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCAGCCGGCGCATGGCCTGGCAGATc       >  6:301524/1‑148 (MQ=255)
                                                                                                                                           caccCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCAGCCGGCGCATGGCCTGGCAGATCGAc    <  3:303182/149‑1 (MQ=255)
                                                                                                                                            ccccAGCGTGATCGAGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGt                                                      <  2:22354/97‑1 (MQ=255)
                                                                                                                                            ccccAGCGTGATCGAGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGt                                                      >  1:22354/2‑98 (MQ=255)
                                                                                                                                            ccccAGCGTGATCGAGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCAGCCGGCGCATGGCCTGGCAGAt        <  5:153108/143‑1 (MQ=255)
                                                                                                                                            ccccAGCGTGATCGAGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCAGCCGGCGCATGGCCTGGCAGAt        >  6:153108/2‑144 (MQ=255)
                                                                                                                                             cccAGCGTGATCGAGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCAGCCGGCGCATGGCCTGGCAGATCGActg  >  7:178998/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
GCCAGCTTGTAGCGGGCCGACAGCGGCAGGTGGCGTAGGATCCACAAGCTCAGCGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCAGCCGGCGCATGGCCTGGCAGATCGACTG  >  NC_002947/3300452‑3300741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑