Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,300,605 A→G 9.7% V101A (GTG→GCG) ‡ lpxH ← UDP‑2,3‑diacylglucosamine hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,300,6050AG 9.7% 166.9 / 7.5 62V101A (GTG→GCG) ‡lpxHUDP‑2,3‑diacylglucosamine hydrolase
Reads supporting (aligned to +/- strand):  ref base A (30/26);  new base G (3/3);  total (33/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01

GGGCCGACAGCGGCAGGTGGCGTAGGATCCACAAGCTCAGCGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCAGCCGGCGCATGGCCTGGCAGATCGACTGCTGGAAGGGC  >  NC_002947/3300465‑3300751
                                                                                                                                            |                                                                                                                                                  
gggCCGACAGCGGCAGGTGGCGTAGGATCCACAAGCTCAGCGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTgggg                                                                                                                                            <  4:165558/149‑1 (MQ=255)
  gCCGACAGCGGCAGGTGGCGTAGGATCCACAAGCTCAGCGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCGTGATCGAGCTgggtgg                                                                                                                                          <  1:89787/149‑4 (MQ=255)
      aCAGCGGCAGGTGGCGTAGGATCCACAAGCTCAGCGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGtt                                                                                                                                       >  6:113159/1‑148 (MQ=255)
       cAGCGGCAGGTGGCGTAGGATCCACAAGCTCAGCGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCATCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCa                                                                                                                                     <  1:7918/149‑1 (MQ=255)
          cGGCAGGTGGCGTAGGATCCACAAGCTCAGCGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACa                                                                                                                                  <  2:85426/149‑1 (MQ=255)
               ggTGGCGTAGGATCCACAAGCTCAGCGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTg                                                                                                                             <  7:209525/149‑1 (MQ=255)
                   gCGTAGGATCCACAAGCTCAGCGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAg                                                                                                                          <  8:268102/148‑1 (MQ=255)
                     gTAGGATCCACAAGCTCAGCGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGccc                                                                                                                       >  4:273066/1‑149 (MQ=255)
                             cacaAGCTCAGCGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGc                                                                                                                         <  4:308679/139‑1 (MQ=255)
                             cacaAGCTCAGCGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGc                                                                                                                         >  3:308679/1‑139 (MQ=255)
                             cacaAGCTCAGCGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGCGCCCTGGTGCACAGCGTGTCCCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCttt                                                                                                               <  1:116813/149‑1 (MQ=255)
                                   cTCAGCGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCaaaa                                                                                                         <  3:273066/149‑1 (MQ=255)
                                    tCAGCGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACCGCGTGCAGCCCGCAGCTTTGCAAAAg                                                                                                        >  7:295534/1‑149 (MQ=255)
                                      aGCGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCGACAGCGTGCAGCCCGCAGCTTTGCAAAAGGc                                                                                                      >  6:350026/1‑149 (MQ=255)
                                        cGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCa                                                                                                                    <  4:25885/133‑1 (MQ=255)
                                        cGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCa                                                                                                                    >  3:25885/1‑133 (MQ=255)
                                               cGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAAt                                                                                             >  2:287696/1‑149 (MQ=255)
                                                gTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCGACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATc                                                                                            <  5:350026/149‑1 (MQ=255)
                                                gTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAAt                                                                                             >  2:300165/1‑148 (MQ=255)
                                                     aGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGaa                                                                                       <  8:205745/149‑1 (MQ=255)
                                                         ggcgCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCa                                                                                   >  4:87154/1‑149 (MQ=255)
                                                            gcATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACgg                                                                                >  2:118865/1‑149 (MQ=255)
                                                                   cATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTg                                                                             <  1:287696/145‑1 (MQ=255)
                                                                   cATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGc                                                                            <  1:300165/146‑1 (MQ=255)
                                                                    aTGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGtt                                                                              <  7:52648/143‑1 (MQ=255)
                                                                    aTGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATg                                                                        >  1:134791/1‑149 (MQ=255)
                                                                     tGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGc                                                                       <  1:118865/149‑1 (MQ=255)
                                                                      gTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCa                                                                      >  2:101950/1‑149 (MQ=255)
                                                                           ccAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAgg                                                                 >  8:22072/1‑149 (MQ=255)
                                                                            cAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAgg                                                                 >  2:170107/1‑148 (MQ=255)
                                                                                  gcgGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCaa                                                                                                           <  4:343620/100‑1 (MQ=255)
                                                                                  gcgGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCaa                                                                                                           >  3:343620/1‑100 (MQ=255)
                                                                                       tGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAAt                                                                                             >  2:88281/1‑109 (MQ=255)
                                                                                       tGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAAt                                                                                             <  1:88281/109‑1 (MQ=255)
                                                                                          acaGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCa                                                  <  7:22072/149‑1 (MQ=255)
                                                                                          acaGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCa                                                  >  5:20483/1‑149 (MQ=255)
                                                                                              ggTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGt                                              >  4:335526/1‑149 (MQ=255)
                                                                                                    gCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCTACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCtgt                                        <  8:295534/149‑3 (MQ=255)
                                                                                                    gCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCtgg                                        >  1:128127/1‑147 (MQ=255)
                                                                                                      cATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCAGc                                      >  6:289573/1‑149 (MQ=255)
                                                                                                          cATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTATATGGCCGTGCCACTGTCGCTCa                                        <  7:227855/143‑1 (MQ=255)
                                                                                                          cATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCa                                        >  8:227855/1‑143 (MQ=255)
                                                                                                           aTCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGACATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCAGCCGgcg                                 >  3:228331/1‑149 (MQ=255)
                                                                                                           aTCAGCAGCACCTCATCACCACCCAGCAACATCACCCAGTGCTCCTTCAACATCGTCCAGCCCCCAGATAAGCAAAAGGCCTGGCCAATCATGAACTCACCGTTGCCATGAATCAGGTAAATCGACGTCCCACTCACGCTCAGccgccg                                 >  6:303401/1‑149 (MQ=255)
                                                                                                               gcagcaCCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTgga                                                                                                 >  8:235221/1‑80 (MQ=255)
                                                                                                               gcagcaCCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCAGCCGGCGCATg                             <  5:113159/149‑1 (MQ=255)
                                                                                                                cagcaCCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCAGCCGgc                                  <  8:188969/143‑1 (MQ=255)
                                                                                                                cagcaCCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCAGCCGgc                                  >  7:188969/1‑143 (MQ=255)
                                                                                                                 agcaCCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCAGCCGGCGCATGGc                           >  3:84480/1‑149 (MQ=255)
                                                                                                                    aCCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCAGCCGGCGCATGGCCTg                        >  2:257210/1‑149 (MQ=255)
                                                                                                                     ccTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCAGCCGGCGCATGGCCTgg                       >  4:303182/1‑149 (MQ=255)
                                                                                                                      cTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCAGCCGGCGCATGGCCTGGc                      <  1:101950/149‑1 (MQ=255)
                                                                                                                            accaccCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCAGCCGGCGCATGGCCTGGCAGATc                 >  6:301524/1‑148 (MQ=255)
                                                                                                                              caccCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCAGCCGGCGCATGGCCTGGCAGATCGAc              <  3:303182/149‑1 (MQ=255)
                                                                                                                               ccccAGCGTGATCGAGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGt                                                                <  2:22354/97‑1 (MQ=255)
                                                                                                                               ccccAGCGTGATCGAGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGt                                                                >  1:22354/2‑98 (MQ=255)
                                                                                                                               ccccAGCGTGATCGAGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCAGCCGGCGCATGGCCTGGCAGAt                  >  6:153108/2‑144 (MQ=255)
                                                                                                                               ccccAGCGTGATCGAGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCAGCCGGCGCATGGCCTGGCAGAt                  <  5:153108/143‑1 (MQ=255)
                                                                                                                                cccAGCGTGATCGAGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCAGCCGGCGCATGGCCTGGCAGATCGActg            >  7:178998/1‑149 (MQ=255)
                                                                                                                                        gATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCAGCCGGCGCATGGCCTGGCAGATCGACTGCTGGaa      <  5:301524/147‑1 (MQ=255)
                                                                                                                                        gATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCAGCCGGCGCATGGCCTGGCAGATCAACTGCTGGAAg     <  5:289573/148‑1 (MQ=255)
                                                                                                                                          tCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCAGCCGGCGCATGGCCTGGCAGATCGACTGCTGGAAGGGc  >  4:157055/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                                  
GGGCCGACAGCGGCAGGTGGCGTAGGATCCACAAGCTCAGCGGATTGCGTAGCAGGCGGCGCATCTTCATGTAGCCCAGGTCGCGGGTGCACAGGGTGTCGCCATGCATCAGCAGCACCTGTTCACCACCCAGCTCGATCACGCTGGGGTCGTTCAACAGCGTGCAGCCCGCAGCTTTGCAAAAGGCCTGGCCAATCAGGAAGTCACGGTTGCCATGCATCAGGTAGATGGCCGTGCCACTGTCGCTCAGCCGGCGCATGGCCTGGCAGATCGACTGCTGGAAGGGC  >  NC_002947/3300465‑3300751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑