Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,413,232	C→T	6.6%	A81V (GCC→GTC)	PP_3026 →	recombinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,413,232	0	C	T	6.6%	172.5 / 2.7	60	A81V (GCC→GTC)	PP_3026	recombinase
Reads supporting (aligned to +/- strand):  ref base C (26/30);  new base T (2/2);  total (28/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01

AAGTCCTGGGCTGCAGCAAGGGGGCTTACGCTAGATGAGTCGCTTTCTTTGAGGGATGAGGGCCTGTCTGCATTCCACCAACGGCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGAACCCATACAGGCGCAAGCGCAGCTGGCTCAGATCATCAACGCGGGGATTACCGTGGTCACTGCGAGTGA‑CGGCCGTGAGTACAACAGGGAAAGGCTGA  >  NC_002947/3413087‑3413378                                                                                                                                                  |                                                                                                                                                    aaGTCCTGGGCTGCAGCAAGGGGGCTTACGCTAGATGAGTCGCTTTCTTTGAGGGATGAGGGCCTGTCTGCATTCCACCAACGGCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCccggccgg                                                                                                                                                  >  5:190015/1‑149 (MQ=255)      cTGGGCTGCAGCAAGGGGGCTTACGCTAGATGAGTCGCTTTCTTTGAGGGATGAGGGCCTGTCTGCATTCCACCAACGGCACATTAAACAAGGTGCTTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCg                                                                                                                                             >  6:213544/1‑149 (MQ=255)       tGGGCTGCAGCAAGGGGGCTTACGCTAGATGAGTCGCTTTCTTTGAGGGATGAGGGCCTGTCTGCATTCCACCAACGGCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGt                                                                                                                                            >  6:27935/1‑149 (MQ=255)           cTGCAGCAAGGGGGCTTACGCTAGATGAGTCGCTTTCTTTGAGGGATGAGGGCCTGTCTGCATTCCACCAACGGCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTtg                                                                                                                                        <  6:333359/149‑1 (MQ=255)           cTGCAGCAAGGGGGCTTACGCTAGATGAGTCGCTTTCTTTGAGGGATGAGAGCCTGTCTGCATTCCACCAACGGCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTtg                                                                                                                                        >  1:89803/1‑149 (MQ=255)              cagcaAGGGGGCTTACGCTAGATGAGTCGCTTTCTTTGAGGGATGAGGGCCTGTCTGCATTCCACCAACGGCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTtggtg                                                                                                                                     >  7:11183/1‑149 (MQ=255)              cagcaAGGGGGCTTACGCTAGATGAGTCGCTTTCTTTGAGGGATGAGGGCCTGTCTGCATCCCACCAACGGCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTtgg                                                                                                                                       <  7:192945/147‑1 (MQ=255)               agcaAGGGGGCTTACGCTAGATGAGTCGCTTTCTTTGAGGGATGAGGGCCTGTCTGCATTCCCCCAACGGCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGTCGGCTCCGTCTtggtgg                                                                                                                                    >  7:251537/1‑149 (MQ=255)               agcaAGGGGGCTTACGCTAGATGAGTCGCTTTCTTTGAGGGATGAGGGCCTGTCTGCATTCCACCAACGGCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTtggtgg                                                                                                                                    <  3:237857/149‑1 (MQ=255)               agcaAGGGGGCTTACGCTAGATGAGTCGCTTTCTTTGAGGGATGAGGGCCTGTCTGCATTCCACCAACGGCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTtg                                                                                                                                        <  4:90263/145‑1 (MQ=255)               agcaAGGGGGCTTACGCTAGATGAGTCGCTTTCTTTGAGGGATGAGGGCATGTCTGCATTCCACCAACGGCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGTCGGCTCCGTCTtggtgg                                                                                                                                    >  7:256434/1‑149 (MQ=255)               agcaAGGGGGCTTACGCTAGATGAGTCGCTTTCTTTGAGGGAAGAGGGCCTGTCTGCATTCCACCAACGGCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTtg                                                                                                                                        >  3:90263/1‑145 (MQ=255)                gcaAGGGGGCTTACGCTAGATGAGTCGCTTTCTTTGAGGGATGAGGGCCTGTCTGCATTCCACCAACGGCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTggtggt                                                                                                                                   >  1:347798/1‑149 (MQ=255)                 caAGGGGGCTTACTCTAGATGAGTCGCTTTCTTTGTGGGATGAGGGCCTTTCTGCATTCCACCAACTGCACATTAAACTAGGTGCCTTAGGAGTTTTCCTTCAGGCTGTTGACGAGGGTCGCATCCCGGCCGGCTCCGTCTTGGTGGtt                                                                                                                                  <  1:9953/149‑1 (MQ=255)                 caAGGGGGCTTACGCTAGATGAGTCGCTTTCTTTGAGGGATGAGGGCCTGTCTGCATTCCACCAACGGCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGtt                                                                                                                                  >  2:231521/1‑149 (MQ=255)                 caAGGGGGCTTACGCTAGATGAGTCGCTTTCTTTGAGGGATGAGGGCCTGTCTGCATTCCACCAACGGCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGtt                                                                                                                                  <  3:181067/149‑1 (MQ=255)                  aaGGGGGCTTACGCTAGATGAGTCGCTTTCTTTGAGGGATGAGGGCCTGTCTGCATTCCACCAACGGCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGtt                                                                                                                                  >  3:128508/1‑148 (MQ=255)                   aGGGGGCTTACGCTAGATGAGTCGCTTTCTTTGAGGGATGAGGGCCTGTCTGCATTCCACCAACGGCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGa                                                                                                                                <  5:27935/149‑1 (MQ=255)                            gttcTAGATGAGTCGCGTGCGTTGAGGGATGAGGGCCTGTCTGCATTCCACCAACGGCACATTAAACAAGGTGCCGTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCGGGCCGGGTCCGTCTTGGTGGTTGAAGGCCTGGa                                                                                                                       <  4:259488/146‑1 (MQ=255)                            aCGCTAGATGAGTCGCTTTCTTTGAGGGATGAGGGCCTGTCTGCATTCCACCAACGGCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTgg                                                                                                                        <  1:350281/148‑1 (MQ=255)                            aCGCTAGATGAGTCGCTTTCTTTGAGGGATGAGGGCCTGTCTGCATTCCACCAACGGCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTgg                                                                                                                        <  5:135615/148‑1 (MQ=255)                                  gATGAGTCGCTTTCTTTGAGGGATGAGGGCCTGTCTGCATTCCACCAACGGCACATTAAACTAGGTGCCTTAGGCGTTTTCCTTCAGGCTCTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGa                                                                                                                       >  3:259488/1‑143 (MQ=255)                                          gCTTTCTTTGAGGGATGAGGGCCTGTCTGCATTCCACCAACGGCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCgg                                                                                                          <  2:89803/148‑1 (MQ=255)                                           ctttctttGAGGGATGAGGGCCTGTCTGCATTCCACCAACGGCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGc                                                                                                        <  7:335067/149‑1 (MQ=255)                                           ctttctttGAGGGATGAGGGCCTGTCTGCATTCCACCAACGGCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGc                                                                                                        >  1:340899/1‑149 (MQ=255)                                           ctttctttGAGGGATGAGGGCCTGTCTGCATTCCACCAACGGCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGc                                                                                                        <  1:329081/149‑1 (MQ=255)                                            tttctttGAGGGATGAGGGCCTGTCTGCATTCCACCAACGGCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCccggccgg                                                                                                                                                  <  3:77724/106‑1 (MQ=255)                                            tttctttGAGGGATGAGGGCCTGTCTGCATTCCACCAACGGCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCccggccgg                                                                                                                                                  >  4:77724/1‑106 (MQ=255)                                                 ttGAGGGATGATGGCCTGTCTGCATTCCACCAACGGCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGAAcc                                                                                                  >  7:273242/1‑149 (MQ=255)                                                 ttGAGGGATGAGGGCCTGTCTGCATTCCACCAACGGCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGAAcc                                                                                                  >  5:205873/1‑149 (MQ=255)                                                  tGAGGGATGAGGGCCTGTCTGCATTCCAGCAACGTCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGaa                                                                                                    <  6:190015/146‑1 (MQ=255)                                                  tGAGGGATGAGGGCCTGTCTGCATTCCACCAACGGCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGaa                                                                                                    <  4:128508/146‑1 (MQ=255)                                                      ggATGAGGGCCTGTCTGCATTCCACCAACGGCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGAACCCATAc                                                                                             <  2:340899/149‑1 (MQ=255)                                                                   tCTGCATTCCACCAACGGCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGAACCCATACAGGCGCAAGCGCa                                                                                <  1:197944/149‑1 (MQ=255)                                                                       cATTCCACCAACGGCACATTAAACAAGGTGCTTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGAACCCATACAGGCGCAAGCGCAg                                                                               <  5:213544/146‑1 (MQ=255)                                                                       cATTCCACCAACGGCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGAACCCATACAGGCGCAAGCGCAGCTg                                                                            >  5:220977/1‑149 (MQ=255)                                                                        aTTCCACCAACGGCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGAACCCATACAGGCGCAAGCTCAGCTg                                                                            >  4:283355/1‑148 (MQ=255)                                                                            caccaACGGCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCg                                                                                                       >  1:91153/1‑117 (MQ=255)                                                                            caccaACGGCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCg                                                                                                       <  2:91153/117‑1 (MQ=255)                                                                             accaACGGCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGAACCCATACAGGCGCAAGCGCAGCTGGCTCAg                                                                      <  6:205873/149‑1 (MQ=255)                                                                                        aTTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGAACCCATACAGGCGCAAGCGCAGCTGGCTCAGATCATCAAcgc                                                           >  1:82666/1‑149 (MQ=255)                                                                                           aaaCAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGAACCCATACAGGCGCAAGCGCAGCTGGCTCAGATCATCAACGCgg                                                         <  8:11183/148‑1 (MQ=255)                                                                                             aCAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGAACCCATACAGGCGCaa                                                                                     <  6:326996/118‑1 (MQ=255)                                                                                             aCAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGAACCCATACAGGCGCaa                                                                                     >  5:326996/1‑118 (MQ=255)                                                                                             aCAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGAACCCATACAGGCGCAAGCGCAGCTGGCTCAGATCATCAACGCGGGGa                                                      <  2:347798/149‑1 (MQ=255)                                                                                               aaGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGAACCCATACAGGCGCAAGCGCAGCTGGCTCAGATCATCAACGCGGGGAt                                                     >  7:322652/1‑148 (MQ=255)                                                                                                    gCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGAACCCATACAGGCGCAAGCGCAGCTGGCTCAGATCATCAACGCGGGGa                                                      >  3:146601/1‑142 (MQ=255)                                                                                                    gCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGAACCCATACAGGCGCAAGCGCAGCTGGCTCAGATCATCAACGCGGGGa                                                      <  4:146601/142‑1 (MQ=255)                                                                                                     ccTTATGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGTCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGAACCCATACAGGCGCAAGCGCAGCTGGCGCAGATCATCAACGCGGGGATTACCGTg                                              <  8:256434/149‑1 (MQ=255)                                                                                                     ccTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGTCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGAACCCATACAGGCGCAAGCGCAGCGGGCTCAGATCATCAACGCGGGGATTACCGTg                                              <  8:251537/149‑1 (MQ=255)                                                                                                             gTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGAACCCATACAGGCGCAAGCGCAGCTGGCTCAGATCATCAACGCGGGGATTACCGTGGTCACTGc                                      >  1:82381/1‑149 (MQ=255)                                                                                                                    ttCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGAACCCATACAGGCGCAAGCGCAGCTGGCTCAGATCATCAACGCGGGGATTACCGTGGTCACTGCGAGTGA‑c                              >  6:43969/1‑149 (MQ=255)                                                                                                                             ttGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGAACCCATACAGGCGCAAGCGCAGCTGGCTCAGATCATCAACGCGGGGATTACCGTGGTCACTGCGAGTGA‑CGGCCGTGa                      <  2:82666/148‑1 (MQ=255)                                                                                                                               gACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGAACCCATACAGGCGCAAGCGCAGCTGGCTCAGATCATCAACGCGGGGATTACCGTGGt                                            <  5:233447/125‑1 (MQ=255)                                                                                                                               gACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGAACCCATACAGGCGCAAGCGCAGCTGGCTCAGATCATCAACGCGGGGATTACCGTGGt                                            >  6:233447/1‑125 (MQ=255)                                                                                                                               gACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGAACCCATACAGGCGCAAGCGCAGCTGGCTCAGATCATCAACGCGGGGATTACCGTGGTCACTGCGAGTGA‑CGGCCGTGAGTa                   >  2:152598/1‑149 (MQ=255)                                                                                                                                aCGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGAACCCATACAGGCGCAAGCGCAGCTGGCTCAGATCATCAACGCGGGGATTACCGTGGTCACTGCGAGTGA‑CGGCCGTGAGTa                   <  1:152598/148‑1 (MQ=255)                                                                                                                                       gcgcATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGAACCCATACAGGCGCAAGAGCAGCTGGCTCAGATCATCAACGCGGGGATTACCGTGGTCACTGCGAGTGA‑CGGCCGTGAGTACAACAgg            >  7:182816/1‑148 (MQ=255)                                                                                                                                         gcATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGAACCCATACAGGCGCAAGCGCAGCTGGCTCAGATCATCAACGCGGGGATTACCGTGGTCACTGCGAGTGA‑CGGCCGTGa                      <  3:46645/136‑1 (MQ=255)                                                                                                                                         gcATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGAACCCATACAGGCGCAAGCGCAGCTGGCTCAGATCATCAACGCGGGGATTACCGTGGTCACTGCGAGTGA‑CGGCCGTGa                      >  4:46645/1‑136 (MQ=255)                                                                                                                                          cATCCCGGCCGGCTCCTTCTTGGTGGTTGTTTGCCTGGACCGTCTGAGCCGGGGGGAACCCATACAGGCGCAAGCGCAGCTGGCTCAGATCATGAAGGCGGGGATTACCGTGGTGACTGCGAGTGT‑CGGCCGTGAGTTCAACAGGGaaa        <  8:182816/149‑1 (MQ=255)                                                                                                                                          cATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGAACCCATACAGGCGCAAGCGCAGCTGGCTCAGATCATCAACGCGGGGATTACCGTGGTCACTGCGAGTGA‑CGGCCGTGAGTACAACAGGGaaa        <  6:220977/149‑1 (MQ=255)                                                                                                                                           aTCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGAACCCATACAGGCGCAAGCGCAGCTGGCTCAGATCATCAACGCGGGGATTACCGTGGTCACTGCGAGTGA‑CGGCCGTGAGTACAACAGGGAAAg       >  2:3659/1‑149 (MQ=255)                                                                                                                                              ccggccggCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGAACCCATACAGGCGCAAGCGCAGCTGGCTCAGATCATCAACGCGGGGATTACCGTGGTCACTGCGAGTGACCGGCCGTGAGTACAACAGGGAAAGGc     <  1:3659/149‑1 (MQ=255)                                                                                                                                              ccggccggCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGAACCCATACAGGCGCAAGCGCAGCTGGCTCAGATCATCAACGCGGGGATTACCGTGGTCACTGCGAGTGA‑CGGCCGTGAGTACAACAGGGAAAGGCt    >  5:217325/1‑149 (MQ=255)                                                                                                                                                ggccggcTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGAACCCATACAGGCGCAAGCGCATCTGGCTCAGATCATCAACGCGGGGATTACCGTGGTCACTGCGAGTGA‑CGGCCGTGAGTACAACAGGGAAAGGCTGa  <  8:273242/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                    AAGTCCTGGGCTGCAGCAAGGGGGCTTACGCTAGATGAGTCGCTTTCTTTGAGGGATGAGGGCCTGTCTGCATTCCACCAACGGCACATTAAACAAGGTGCCTTAGGCGTTTTCCTTCAGGCTGTTGACGAGGGGCGCATCCCGGCCGGCTCCGTCTTGGTGGTTGAAGGCCTGGACCGTCTGAGCCGGGCGGAACCCATACAGGCGCAAGCGCAGCTGGCTCAGATCATCAACGCGGGGATTACCGTGGTCACTGCGAGTGA‑CGGCCGTGAGTACAACAGGGAAAGGCTGA  >  NC_002947/3413087‑3413378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑