Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,422,822 G→T 7.3% W327L (TGG→TTG)  PP_3038 → TraC domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,422,8220GT 7.3% 169.4 / 3.7 55W327L (TGG→TTG) PP_3038TraC domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (27/24);  new base T (2/2);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01

TTGTGGGTGGCCAGGTAGTCGGCCCGATCTTCTCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAATGATGAGCGCTGGAGCGGGGTGATCGGTTACAACGCCTTCAGCTCCAAGATCATGCGCCTTCGTGCGGCGCCT  >  NC_002947/3422675‑3422970
                                                                                                                                                   |                                                                                                                                                    
gtgtgGGTGGGCAGGTAGTCGGCCCGATCTTCTCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTgg                                                                                                                                                     <  2:93322/148‑1 (MQ=255)
   tgggtggCCAGGTAGTCGGCCCGATCTTCTCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAg                                                                                                                                                  <  5:293839/149‑1 (MQ=255)
    gggtggCCAGGTAGTCGGCCCGATCTTCTCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACTGGCTACCGGTGGCTGGAAgg                                                                                                                                                 <  6:13389/149‑1 (MQ=255)
    gggtggCCAGGTAGTCGGCCCGATCTTCTCGGCTGAGCCTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCTTTCGCCGGCAAGTCACGGCAGTTATCAAGCCACCGGCTACCGGTGGCTGGAAg                                                                                                                                                  >  4:133669/1‑148 (MQ=255)
       tggCCAGGTAGTCGGCCCGATCTTCTCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACa                                                                                                                                              <  3:75779/149‑1 (MQ=255)
                  tCGGCCCGATCTTCTCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAg                                                                                                                                    >  1:291176/1‑148 (MQ=255)
                     gCCCGATCTTCTCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACt                                                                                                                                >  3:188950/1‑149 (MQ=255)
                        cGATCTTCTCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACt                                                                                                                                >  2:166311/1‑146 (MQ=255)
                         gATCTTCTCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTga                                                                                                                              >  3:120346/1‑147 (MQ=255)
                          aTCTTCTCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGagag                                                                                                                           >  3:67088/1‑149 (MQ=255)
                           tcttctCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCCAGCCACCGGTAGCCGGTGGCTTGat                                                                                                                                                   <  1:61803/124‑2 (MQ=255)
                           tcttctCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCCAGCCACCGGTAGCCGGTGGCTTGat                                                                                                                                                   >  2:61803/1‑123 (MQ=255)
                              tctcGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGaa                                                                                                                                                   >  1:351743/1‑121 (MQ=255)
                              tctcGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGaa                                                                                                                                                   <  2:351743/121‑1 (MQ=255)
                               ctcGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAgtggtg                                                                                                                      >  6:314958/1‑149 (MQ=255)
                               ctcGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGGGGCTGGAAGGACATGCTGGCCAGGACTGAGAgtggt                                                                                                                       <  6:366827/148‑1 (MQ=255)
                                tcGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGc                                                                                                                     >  4:255074/1‑149 (MQ=255)
                                  ggCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGTAGCCGGTGGCTTGatcact                                                                                                                                               >  5:260584/1‑116 (MQ=255)
                                  ggCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGTAGCCGGTGGCTTGatcact                                                                                                                                               <  6:260584/121‑6 (MQ=255)
                                  ggCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGcc                                                                                                                    <  5:314958/148‑1 (MQ=255)
                                   gCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCt                                                                                                                  <  1:166311/149‑1 (MQ=255)
                                               gTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCatat                                                                                                      <  4:67088/149‑1 (MQ=255)
                                                tttaaTGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATg                                                                                                     >  3:103308/4‑149 (MQ=255)
                                                ttAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATg                                                                                                     >  8:15360/1‑149 (MQ=255)
                                                ttAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATg                                                                                                     <  7:15360/149‑1 (MQ=255)
                                                       gACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAAc                                                                                               <  1:341709/148‑1 (MQ=255)
                                                       gACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACg                                                                                              >  2:133291/1‑149 (MQ=255)
                                                              ttCAACGACATGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACt                                                                                       >  4:239473/1‑149 (MQ=255)
                                                               tCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACt                                                                                       >  3:222458/1‑148 (MQ=255)
                                                                cAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGa                                                                                     >  1:43861/1‑149 (MQ=255)
                                                                   cGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGc                                                                                                    <  2:77091/131‑1 (MQ=255)
                                                                   cGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGc                                                                                                    >  1:77091/1‑131 (MQ=255)
                                                                   cGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATcc                                                                                  <  4:120346/149‑1 (MQ=255)
                                                                            ttGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAatg                                                                         <  3:133669/149‑1 (MQ=255)
                                                                               cgcTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAatgatg                                                                      <  3:255074/149‑1 (MQ=255)
                                                                                    aaGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAatgat                                                                       <  8:43182/143‑1 (MQ=255)
                                                                                    aaGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAatgat                                                                       >  7:43182/1‑143 (MQ=255)
                                                                                    aaGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAATGATGAgc                                                                   <  3:239473/147‑1 (MQ=255)
                                                                                     aGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAATGATGAGCGCt                                                                <  4:188950/149‑1 (MQ=255)
                                                                                         cTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAATGATGAGCGCTGGAg                                                            >  1:252597/1‑149 (MQ=255)
                                                                                          ttGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAATGATGAGCGCTGGAGc                                                           >  1:346510/1‑149 (MQ=255)
                                                                                                       gcGGCAAGTCACGGGAGTGATCAAGCCACCTGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCTGAACGTCGAACTGATCCTGGCCAATGATGAGCGCTGGAGCGGGGTGATCGGtt                                              <  4:87231/148‑1 (MQ=255)
                                                                                                       ccGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAATGATGAGCGCTGGAGCGGGGTGATCGGtt                                              <  4:222458/149‑1 (MQ=255)
                                                                                                               tCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAATGATGAGCGCTGGAGCGGGGTGATCGGTTa                                             >  3:318067/1‑142 (MQ=255)
                                                                                                               tCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAATGATGAGCGCTGGAGCGGGGTGATCGGTTa                                             <  4:318067/142‑1 (MQ=255)
                                                                                                               tCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAATGATGAGCGCTGGAGCGGGGTGATCGGTTACAACGcc                                      >  4:69189/1‑149 (MQ=255)
                                                                                                                 aCGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAATGATGAGCGCTGGAGCGGGGTGATCGGTTACAACGCCtt                                    <  2:43861/149‑1 (MQ=255)
                                                                                                                 aCGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAATGATGAGCGCTGGAGCGGGGTGATCGGTTACAACGCCtt                                    >  7:70640/1‑149 (MQ=255)
                                                                                                                   ggCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAATGATGAGCGCTGGAGCGGGGTGATCGGTTACAACGCCtt                                    >  2:278638/1‑147 (MQ=255)
                                                                                                                         gATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGCGTGGTGCCCTGATCGCCCATATGAAGAACGTCGAACTGAACCTGGCCAATGATGAGCGCTGGAGCGGGCTGATACGTTACAACGCCTGCAGcaca                             >  8:313829/1‑145 (MQ=255)
                                                                                                                         gATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAATGATGAGCGCTGGAGCGGGGTGATCGGTTACAACGCCTTCAGCTcc                             <  4:103308/148‑1 (MQ=255)
                                                                                                                          ttccaGCCACCGGTAGCCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAATGATGAGCGCTGGAGCGGGGTGATCGGTTACAACGCCTTCAGCTcc                             >  1:50370/5‑147 (MQ=255)
                                                                                                                             caGCCACCGGTAGCCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAATGATGAGCGCTGGAGCGGGGTGATCGGTTACAACGCCTTCAGCTcc                             >  6:211534/2‑144 (MQ=255)
                                                                                                                                  aCCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAATGATGAGCGCTGGAGCGGGGTGATCGGTTACAACGCCTTCAGCTCCAAGATCATgc                   <  2:346510/149‑1 (MQ=255)
                                                                                                                                          ccGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAATGATGAGCGCTGGAGCGGGGTGATCGGTTACAACGCCTTCAGCTCCAAGATCATGCGCCTTc             <  1:133291/147‑1 (MQ=255)
                                                                                                                                                  tGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAATGATGAGCGCTGGAGCGGGGTGATCGGTTACAACGCCTTCAGCTCCAAGATCATGCGCCTTCGTGCGGCGcc   >  5:198273/1‑149 (MQ=255)
                                                                                                                                                   ggAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAATGATGAGCGCTGGAGCGGGGTGATCGGTTACAACGCCTTCAGCTCCAAGATCATGCGCCTTCGTGCGGCGCCt  >  6:123777/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TTGTGGGTGGCCAGGTAGTCGGCCCGATCTTCTCGGCTGAGCGTGAGGTTAAATGGACCGACTTCAACGACCTGCATTGCGCTGAAGGCCTTGAGGCCGTTCGCCGGCAAGTCACGGCAGTGATCAAGCCACCGGCTACCGGTGGCTGGAAGGACATGCTGGCCAGGACTGAGAGTGGTGCCCTGATCGCCCATATGCAGAACGTCGAACTGATCCTGGCCAATGATGAGCGCTGGAGCGGGGTGATCGGTTACAACGCCTTCAGCTCCAAGATCATGCGCCTTCGTGCGGCGCCT  >  NC_002947/3422675‑3422970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑