| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 28,270 | A→G | 6.9% | C426C (TGT→TGC) | PP_0024 ← | membrane‑associated metal‑dependent hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 28,270 | 0 | A | G | 6.9% | 168.7 / 2.1 | 58 | C426C (TGT→TGC) | PP_0024 | membrane‑associated metal‑dependent hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (25/29); new base G (2/2); total (27/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCAGCGATTCGCCGTGGTCCGAAACGTACATCAGCGCAGTGTCGAACTTGGTGTTGGCCGCCAGGATATCGATGAGATTTGCAGTCACATAATCGGT‑GTAGAGGATTGAGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGCGAGGGGTAGCGCTTGTAGTAGGCCGGGCCGTGACTGCCTTTG‑TAGTGGAGCACCAGGACAGCATCGCCTTCCTGGCGCTTGATGAAGTCTTGCATCT > NC_002947/28128‑28418 | ccAGCGATTCGCCGTGGTCCGAAACGTACATCAGCGCAGTGTCGAACTTGGTGTTGTCCGCCAGGATATCGATGAGATTTGCAGTCACATAATCGGT‑GTAGAGGATTGAGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTACATTTg < 1:43553/149‑1 (MQ=255)ccAGCGATTCGCCGTGGTCCGAAACGTACATCAGCGCAGTGTCGAACTTGGTGTTGGCCGCCAGGATATCGATGAGATTTGCAGTCACATAATCGGT‑GTAGAGGATTGAGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTACATTTg < 3:288172/149‑1 (MQ=255) gATTCGCCGTTGTCCGAAACGTACATCAGCGCAGTGTCGAACTTGGTGTTGGCCGCCAGGATATCGATGATATTTGCAGTCACATAATCGGTAGTAGAGGATTGAGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTACATTTGTCGa < 1:98510/149‑1 (MQ=255) aTTCGCCGTGGTCCGAAACGTACATCAGCGCAGTGTCGAACTTGGTGTTGGCCGCCAGGATATCGATGAGATTTGCAGTCACATAATCGGT‑GTAGAGGATTGAGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTACATTTGTCGAGc < 5:341637/149‑1 (MQ=255) ccGTGGTCCGAAACGTACATCAGCGCATTGTCTAACTTGGTGGTGGCCGCCAGGATATCGATGAGATTTGCAGTAACATAATCGGT‑GTAGAGGATTGAGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTACATTTGTCGAGCT‑CGtt < 3:43596/149‑1 (MQ=255) ccGGGGTCCGAAACGTACATCAGCGCAGTGTCGAACTTGGTGTTTGCCGCCAGGAAATCGATTAGATTTGCAGTCACATAATCGGT‑GTAGAGGATTGAGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTACATTTGTCGAGCT‑CGtt < 8:257753/149‑1 (MQ=255) cGTGGTCCGAAACGTACATCAGCGCAGTGTCGAACTTGGTGTTGGCCGCCAGGATATCGATGAGATTTGCAGTCACATAATCGGT‑GTAGAGGATTGAGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTACATTTGTCGAGCT‑CGTTg < 2:342686/149‑1 (MQ=255) tGGTCCGAAACGTACATCAGCGCAGTGTCGAACTTGGTGTTGGCCGCCAGGATATCGATGAGATTTGCAGTCACATAATCGGT‑GTAGAGGATTGAGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTACATTTGTCGAGCT‑CGTTgg > 7:239444/1‑148 (MQ=255) cGAAACGTACATCAGCGCAGTGTCGAACTTGGTGTTGGCCGCCAGGATATCGATGAGATTTGCAGTCACATAATCGGT‑GTAGAGGATTGAGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTACATTTGTCGAGCT‑CGTTGGTCTCg > 5:117298/1‑148 (MQ=255) gtaaCGTACATCAGCGCATTGTCGAACTTGGTGTTGGCCGCGAGGATATCGATGAGATTTGCAGTCACATAATCGGT‑GTAGAGGATTGAGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTAGATTTGTCGAGCT‑CGTTGGTCTCGca < 4:52663/147‑1 (MQ=255) gTACATCAGCGCAGTGTCGAACTTGGTGTTGGCCGCCAGGATATCGATGAGATTTGCAGTCACATAATCGGT‑GTAGAGGATTGAGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCg > 7:245710/1‑149 (MQ=255) aTCAGCGCAGTGTCGAACTTGGTGTTGGCCGCCAGGATATCGATGAGATTTGCAGTCACATAATCGGT‑GTAGAGGATTGAGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGc > 6:274876/1‑149 (MQ=255) tCAGCGCAGTGTCGAACTTGGTGTTGGCCGCCAGGATATCGATGAGATTTGCAGTCACATAATCGGT‑GTAGAGGATTGAGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGc > 1:214347/1‑148 (MQ=255) gtgtCGAACTTGGTGTTGGCCGCCAGGATATCGATGAGATTTGCAGTCACATAATCGGT‑GTAGAGGATTGAGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCtt < 4:136936/149‑1 (MQ=255) aCTTGGTGTTGGCCGCCAGGATATCGATGAGATTTGCAGTCACATAATCGGT‑GTAGAGGATTGAGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGc < 7:284909/149‑1 (MQ=255) gccgccAGGATATCGATGAGATTTGCAGTCACATAATCGGT‑GTAGAGGATTGAGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGCGAGGGGTAgcg > 4:153335/1‑149 (MQ=255) ccgccAGGATATCGATGAGATTTGCAGTCACATAATCGGT‑GTAGAGGATTGAGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGCGa > 1:153906/1‑139 (MQ=255) ccgccAGGATATCGATGAGATTTGCAGTCACATAATCGGT‑GTAGAGGATTGAGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGCGa < 2:153906/139‑1 (MQ=255) cgccAGGATATCGATGAGATTTGCAGTCACATAATCGGT‑GTAGAGGATTGAGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTAGATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGCGAGGGGTAGCGCt < 5:157603/149‑1 (MQ=255) cgccAGGATATCGATGAGATTTGCAGTCACATAATCGGT‑GTAGAGGATTGAGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGCGAGGGGTAGCGCt > 1:672/1‑149 (MQ=255) aGGATATCGATGAGATTTGCAGTCACATAATCGGT‑GTAGAGGATTGAGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGCGAGGGGTAGCGCTTgta < 3:130819/149‑1 (MQ=255) gATATCGATGAGATTTGCAGTCACATAATCGGT‑GTAGAGGATTGAGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATGGCGAGGGGTAGCGCTTgtag > 6:274040/1‑148 (MQ=255) gATGAGATTTGCAGTCACATAATCGGT‑GTAGAGGATTGAGTTGTCGTAGGCATTGACGACCTCCTCCTGCTTACATTTGTCGAGCT‑CGTTGGTATCGCACACCGGTGAGAATTTCTTGAATTGCGAGGGGTAGCGCTTGTATTAggccg > 8:290035/1‑149 (MQ=255) gATGAGATTTGCAGTCACATAATCGGT‑GTAGAGGATTGAGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGt < 2:192415/106‑1 (MQ=255) gATGAGATTTGCAGTCACATAATCGGT‑GTAGAGGATTGAGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGt > 1:192415/1‑106 (MQ=255) aTTTGCAGTCACATAATCGGT‑GTAGAGGATTGAGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGCGAGGGGTAGCGCTTGTAGTAGGCCGGGCCGt < 8:239444/149‑1 (MQ=255) tttGCAGTCACATAATCGGT‑GTAGAGGATTGAGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTACATTTGt < 8:234577/73‑1 (MQ=255) tttGCAGTCACATAATCGGT‑GTAGAGGATTGAGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTACATTTGt > 7:234577/1‑73 (MQ=255) tCACATAATCGGT‑GTAGAGGATTGAGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGCGAGGGGTAGCGCTTGTAGTAGGCCGGGCCGTGACTGCCt < 5:274876/149‑1 (MQ=255) tCACATAATCGGT‑GTAGAGGATTGAGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTAAATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGCGAGGGGTAGCGCTTGTAGTAGGCCGGGCCGTGACTGCCt > 1:310101/1‑149 (MQ=255) acaTAATCGGT‑GTAGAGGATTGAGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGCGAGGGGTAGCGCTTGTAGTAGGCCGGGCCGTGACTGCCttt > 2:22331/1‑149 (MQ=255) gt‑gtAGAGGATTGAGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGCGAGGGGTAGCGCTTGTAGTAGGCCGGGCCGTGACTGCCTTTG‑TAGTGGAg < 6:117298/149‑1 (MQ=255) agGATTGAGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCg > 8:140555/1‑78 (MQ=255) agGATTGAGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCg < 7:140555/78‑1 (MQ=255) ggATTGAGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGCGAGGGGTAGCGCTTGTAGTAGGCCGGGCCGTGACTGCCTTTG‑TAGTGGAGCACCAg < 2:672/148‑1 (MQ=255) aGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTGCATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGCGAGGGGTAGCGCTTGTAGTAGGCCGGGCCGTGACTGCCTTTG‑TAGTGGAGCACCAGGACAGCa > 3:322222/1‑149 (MQ=255) aGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTGCATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGCGAGGGGTAGCGCTTGTAGTAGGCCGGGCCGTGACTGCCTTTG‑TAGTGGAGCACCAGGACAGCa > 3:317061/1‑149 (MQ=255) aGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTACATTTGTCGAGCTGCGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGCGAGGGGTAGCGCTTGTAGTAGGCCGGGCCGTGACTGCCTTTG‑TTGTGGAGCACCAGGACAGc > 1:84807/1‑149 (MQ=255) ttGTCGTAGGCATTGACCACCTCCTCCTGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGCGAGGGGTAGCGCTTGTAGTAGGCCGGGCCGTGACTGCCTTTG‑TAGTGGAGCACCAGGACAGCATc < 8:245710/149‑1 (MQ=255) tAGGCATTGACCACCTCCTCCTGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGCGAGGGGTAGCGCTTGTAGTAGGCCGGGCCGTGACTGCCTTTG‑TAGTGGAGCACCAGGACAGCATCGCCTTc > 7:6665/1‑149 (MQ=255) gCATTGACCACCTCCTCCTGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGCGAGGGGTAGCGCTTGTAGTAGGCCGGGCCGTGACTGCCTTTG‑TAGTGGAGCACCAGGACAGCATCGCCTTCCTg > 8:223622/1‑149 (MQ=255) accaccTCCTCCTGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGCGa < 1:34331/71‑1 (MQ=255) accaccTCCTCCTGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGCGa > 2:34331/1‑71 (MQ=255) ccaccTCCTCCTGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGCGAGGGGTAGCGCTTGTAGTAGGCCGGGCCGTGACTGCCTTTG‑TAGTGGAGCACCAGGACAGCATCGCCTTCCTGGCGCTtg < 8:6665/149‑1 (MQ=255) caccTCCTCCTGCTTGCATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGCGAGGGGTAGCGCTTGTAGTAGGCCGGGCCGTGACTGCCTTTG‑TAGTGGAGCACCAGGACAGCATCGCCTTCCTGGCGCTtga < 4:322222/149‑1 (MQ=255) caccTCCTCCTGCTTGCATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGCGAGGGGTAGCGCTTGTAGTAGGCCGGGCCGTGACTGCCTTTG‑TAGTGGAGCACCAGGACAGCATCGCCTTCCTGGCGCTtga < 4:317061/149‑1 (MQ=255) caccTCCTCCTGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGCGAgg > 7:51167/1‑71 (MQ=255) caccTCCTCCTGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGCGAgg < 8:51167/71‑1 (MQ=255) caccTCCTCCTGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGCGAGGGGTAGCGCTTGTAGTAGGCCGGGCCGTGACTGCCTTTG‑TAGTGGAGCACCAGGACAGCATCGCCTTCCTGGCGCTtga > 3:306471/1‑149 (MQ=255) cctcctGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGCGAGGGGTAGCGCTTGTAGTAGGCCGGGCCGTGACTGCCTTTG‑TAGTGGAGCACCAGGACa > 1:42572/1‑122 (MQ=255) cctcctGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGCGAGGGGTAGCGCTTGTAGTAGGCCGGGCCGTGACTGCCTTTG‑TAGTGGAGCACCAGGACa < 2:42572/122‑1 (MQ=255) ctcctGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGCGAGGGGTAGCGCTTGTAGTAGGCCGGGCCGTGACTGCCTTTG‑TAGTGGAGCACCAGGACAGCATCGCCTTCCTGGCGCTTGATGAAg < 7:223622/148‑1 (MQ=255) tcctGCATACATTTGTCTAGAT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGCGAGGGGTAGCCCTTGTACTAGGCCGGGCCGTGACTGCCTTTC‑TAGTGCAGCACCAGGACAGCATCGCCTTCCTGGAGCTTGATGAAGTc > 2:2472/1‑149 (MQ=255) ctGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGCGAGGGGTAGCGCTTGTAGTAGGCCGGGCCGTGACTGCCTTTG‑TAGTGGAGCACCAGGACAGCATCGCCTTc < 1:37187/129‑1 (MQ=255) ctGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGCGAGGGGTAGCGCTTGTAGTAGGCCGGGCCGTGACTGCCTTTG‑TAGTGGAGCACCAGGACAGCATCGCCTTc > 2:37187/1‑129 (MQ=255) tGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGCGAGGGGTAGCGCTTGTAGTAGGCCGGGCCGTGACTGCCTTTG‑TAGTGGAGCACCAGGACAGCATCGCCTTCCTGGCGCTTGATGAAGTCTTg > 1:249844/1‑149 (MQ=255) cTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGCGAGGGGTAGCGCTTGTAGTAGGCCGGGCCGTGACTGCCTTTGCTAGTGGAGCACCAGGACAGCATCGCCTTCCTGGCGCTTGATGAAGTCtt < 1:266902/147‑1 (MQ=255) cTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATCGCGAGGGGTAGCGCTTGTAGTAGGCCGGGCCGTGACTGCCTTTG‑TAGTGGAGCACCAGGACAGCATCGCCTTCCTGGCGCTTGATGAAGTCTTg < 5:274040/147‑1 (MQ=255) aCATTTGTCGAGCT‑CGTTGGTCTCGCCCACCGGTGCGAATTTCTTGAAATGCGAGGGGTAGCGCTTGTAGTAGGCCGGGCCGTGACTGCCTTTG‑TAGTGGAGCACCAGGACAGCATCGCCTTCCTGGCGCTTGATGAAGTCTTGCAtct > 1:10253/1‑149 (MQ=255) | CCAGCGATTCGCCGTGGTCCGAAACGTACATCAGCGCAGTGTCGAACTTGGTGTTGGCCGCCAGGATATCGATGAGATTTGCAGTCACATAATCGGT‑GTAGAGGATTGAGTTGTCGTAGGCATTGACCACCTCCTCCTGCTTACATTTGTCGAGCT‑CGTTGGTCTCGCACACCGGTGCGAATTTCTTGAATTGCGAGGGGTAGCGCTTGTAGTAGGCCGGGCCGTGACTGCCTTTG‑TAGTGGAGCACCAGGACAGCATCGCCTTCCTGGCGCTTGATGAAGTCTTGCATCT > NC_002947/28128‑28418 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |