Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,431,783 T→G 5.6% V142V (GTT→GTG)  PP_3047 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,431,7830TG 5.6% 226.4 / 3.3 71V142V (GTT→GTG) PP_3047hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (31/36);  new base G (2/2);  total (33/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

AGGCGGATGAGATCCAGGGCATTCGTGCTTTCGGTGAGCAAACCGAACTGCAGGCAGTGCAGGACGTTGTGAACAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑AATCGAGATCGAGATGGAGCTGGGTACCGCAGGTACCGATGTTCGGGTGAAGTGTGTTGACGCACTGGATGCACAG  >  NC_002947/3431635‑3431921
                                                                                                                                                    |                                                                                                                                           
aGGCGGATGAGATCCAGGGCATTCGTGCTTTCGGTGAGCAAACCGAACTGCAGGCAGTGCAGGACGTTGTGAACAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCgtt                                                                                                                                             <  7:121857/149‑1 (MQ=255)
aGGCGGATGAGATCCAGGGCATTCGTGCTTTCGGTGAGCAAACCGAACTGCAGGCAGTGCAGGACGTTGTGAACAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCgtt                                                                                                                                             <  6:52356/149‑1 (MQ=255)
 ggCGGATGAGATCCAGGGCATTCGTGCTTTCGGTGAGCAAACCGAACTGCAGGCAGTGCAGGACGTTGTGAACAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCgttg                                                                                                                                            >  6:253261/1‑149 (MQ=255)
    ggATGAGATCCAGGGCATTCGTGCTTTCGGTGAGCAAACCGAACTGCAGGCAGTGCAGGACGTTGTGAACAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTc                                                                                                                                         >  1:13924/1‑149 (MQ=255)
      aTGAGATCCAGGGCATTCGTGCTTTCGGTGAGCAAACCGAACTGCAGGCAGTGCAGGACGTTGTGAACAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTg                                                                                                                                       <  8:100131/149‑1 (MQ=255)
        gagaTCCAGGGCATTCGTGCTTTCGGTGAGCAAACCGAACTGCAGGCAGTGCAGGACGTTGTGAACAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGa                                                                                                                                     >  4:46971/1‑149 (MQ=255)
           aTCCAGGGCATTCGTGCTTTCGGTGAGCAAACCGAACTGCAGGCAGTGCAGGACGTTGTGAACAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGAt                                                                                                                                    >  7:118647/1‑147 (MQ=255)
            tCCAGGGCATTCGTGCTTTCGGTGAGCAAACCGAACTGCAGGCAGTGCAGGACGTTGTGAACAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCg                                                                                                                                 >  1:224689/1‑149 (MQ=255)
            tCCAGGGCATTCGTGCTTTCGGTGAGCAAACCGAACTGCAGGCAGTGCAGGACGTTGTGAACAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCg                                                                                                                                 >  8:136416/1‑149 (MQ=255)
            tCCAGGGCATTCGTGCTTTCGGTGAGCAAACCGAACTGCAGGCAGTGCAGGACGTTGTGAACAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCg                                                                                                                                 <  5:253261/149‑1 (MQ=255)
               aGGGCATTCGTGCTTTCGGTGAGCAAACCGAACTGCAGGCAGTGCAGGACGTTGTGAACAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTGGTTCt                                                                                                                                        <  6:54010/139‑1 (MQ=255)
               aGGGCATTCGTGCTTTCGGTGAGCAAACCGAACTGCAGGCAGTGCAGGACGTTGTGAACAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTGGTTCt                                                                                                                                        <  6:48693/139‑1 (MQ=255)
               aGGGCATTCGTGCTTTCGGTGAGCAAACCGAACTGCAGGCAGTGCAGGACGTTGTGAACAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTGGTTCt                                                                                                                                        >  5:54010/1‑139 (MQ=255)
               aGGGCATTCGTGCTTTCGGTGAGCAAACCGAACTGCAGGCAGTGCAGGACGTTGTGAACAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTGGTTCt                                                                                                                                        >  5:48693/1‑139 (MQ=255)
                 ggCATTCGTGCTTTCGTTGAGCAAACAGAACTGCAGGCAGTGCAGGACGTTGTGAACAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCTGACgg                                                                                                                            <  8:88254/149‑1 (MQ=255)
                  gCATTCGTGCTTTCGGTGAGCAAACCGAACTGCAGGCAGTGCAGGACGTTGTGAACAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGt                                                                                                                           <  4:335180/149‑1 (MQ=255)
                   cATTCGTGCTTTCGGTGAGCAAACCGAACTGCAGGCAGTGCAGGACGTTGTGAACAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTa                                                                                                                          <  3:181065/149‑1 (MQ=255)
                           cTTTCGGTGAGCAAACCGAACTGCAGGCAGTGCAGGACGTTGTGAACAAGCGTCTGGTGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGtg                                                                                                                  >  6:117723/1‑149 (MQ=255)
                           cTTTCGGTGAGCAAACCGAACTGCAGGCAGTGCAGGACGTTGTGAACAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCgat                                                                                                                  >  3:15590/1‑147 (MQ=255)
                                 gTGAGCAAACCGAACTGCAGGCAGTGCAGGACGTTGTGAACAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTgccgct                                                                                                             >  2:297590/1‑148 (MQ=255)
                                   gAGCAAACCGAACTGCAGGCAGTGCAGGACGTTGTGAACAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGTGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCaa                                                                                                          >  3:29878/1‑149 (MQ=255)
                                    aGCAAACCGAACTGCAGGCAGTGCAGGACGTTGTGAACAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCaa                                                                                                          >  3:45094/1‑148 (MQ=255)
                                    aGCAAACCGAACTGCAGGCAGTGCAGGACGTTGTGAACAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAat                                                                                                         <  3:105942/149‑1 (MQ=255)
                                      cAAACCGAACTGCAGGCAGTGCAGGACGTTGTGAACAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAatat                                                                                                       >  6:311957/1‑149 (MQ=255)
                                      cAAACCGAACTGCAGGCAGTGCAGGACGTTGTGAACAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAatat                                                                                                       <  5:311957/149‑1 (MQ=255)
                                        aaCCGAACTGCAGGCAGTGCAGGACGTTGTGAACAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCt                                                                                                     <  2:13924/149‑1 (MQ=255)
                                           cGAACTGCAGGCAGTGCAGGACGTTGTGAACAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATg                                                                                                  <  7:136416/149‑1 (MQ=255)
                                                 gcaggcagTGCAGGACGTTGTGAACAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGtt                                                                                            >  7:135084/1‑149 (MQ=255)
                                                  caggcagTGCAGGACGTTGTGAACAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGttt                                                                                           <  8:152645/149‑1 (MQ=255)
                                                   aggcagTGCAGGACGTTGTGAACAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTc                                                                                          <  3:126791/149‑1 (MQ=255)
                                                            aGGACGTTGTGAACAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAg                                                                                 <  2:224689/149‑1 (MQ=255)
                                                                 gTTGTGAACAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑Aat                                                                           >  7:320201/1‑149 (MQ=255)
                                                                  ttGTGAACAAGCGTCTGGTGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑Aatc                                                                          <  5:117723/149‑1 (MQ=255)
                                                                  ttGTGAACAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑Aatc                                                                          >  3:346558/1‑149 (MQ=255)
                                                                      gAACAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑Aatc                                                                          >  4:279701/1‑145 (MQ=255)
                                                                       aaCAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACtt                                                                                    >  6:325934/1‑135 (MQ=255)
                                                                       aaCAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACtt                                                                                    <  5:325934/135‑1 (MQ=255)
                                                                       aaCAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑Aatc                                                                          >  7:10088/1‑144 (MQ=255)
                                                                          aaGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑Aatcgagatc                                                                    >  6:126452/1‑147 (MQ=255)
                                                                               tCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑AAtcgagatcgaga                                                                >  3:16459/1‑146 (MQ=255)
                                                                               tCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑AAtcgagatcgaga                                                                <  4:16459/146‑1 (MQ=255)
                                                                                     gAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGa                                                                                       <  3:75509/118‑1 (MQ=255)
                                                                                     gAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGa                                                                                       >  4:75509/1‑118 (MQ=255)
                                                                                     gAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑AATCGAGATCGAGATGGAGCTgg                                                       >  3:8448/1‑149 (MQ=255)
                                                                                           tcgtcgCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑Aatcgaga                                                                      <  7:103560/128‑1 (MQ=255)
                                                                                           tcgtcgCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑Aatcgaga                                                                      >  8:103560/1‑128 (MQ=255)
                                                                                           tcgtcgCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑AATCGAGATCGAGATGGAGCTGGGTAcct                                                 >  8:107539/1‑148 (MQ=255)
                                                                                           tcgtcgCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑AATCGAGATCGAGATGGAGCTGGGTACCg                                                 >  1:56362/1‑149 (MQ=255)
                                                                                                gCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCCAAATCGAGATCGAGATGGAGCTGGGTACCGCAg                                              <  1:32362/148‑1 (MQ=255)
                                                                                                  cAGTTTGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑AATCGAGATCGAGATGGAGCTGGGTACCGCAGGTAc                                          >  2:41311/1‑149 (MQ=255)
                                                                                                       ggACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑AATCGAGATCGAGATGGAGCTGGGTACCTGCGGTAcccag                                      <  7:107539/148‑4 (MQ=255)
                                                                                                       ggACGCTACGCATGAGGTTCATAGCATGGGCGCGATGATCTGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATTTCTATGATCGTTTCGGTCTTCAGCC‑AATCGAGATCGAGATGGAGCTGGGTACCTGCGGTAcccag                                      <  8:307652/148‑4 (MQ=255)
                                                                                                        gACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑AATCGAGATCGAGATGGAGCTGGGTACCTGCGGTAcccagc                                     <  8:10088/148‑5 (MQ=255)
                                                                                                            cTACGCTTGAGTTTCATGGCATGGGGGCGCTTTACGGCGTTGTTCTGGATGCGGACGGTTGCTCGGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑AATCGAGATCGAGATGGAGCTGGGTACCGCAGGTACCGATGTTCgg                                <  1:342456/149‑1 (MQ=255)
                                                                                                             tACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑AATCGAGATCGAGATGGAGCTGGGTACCGCAGGTACCGATGTTCggg                               >  4:297394/1‑149 (MQ=255)
                                                                                                               cGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑AATCGAGATCGAGATGGAGCTGGGTACCGCAGGTACCGATGTTCggg                               <  3:46971/147‑1 (MQ=255)
                                                                                                                 cATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑AATCGAGATCGAGATGGAGCTGGGTACCGCAGGTACCGATGTTCGGGTGaa                           <  3:297394/149‑1 (MQ=255)
                                                                                                                 cATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑AATCGAGATCGAGATGGAGCTGGGTACCGCAGGTACCGATGTTCGGGTGaa                           <  8:135084/149‑1 (MQ=255)
                                                                                                                  aTGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑AATCGAGATCGAGATGGAGCTGGGTACCGCAGGTACCGATGTTCGGGTGAAg                          >  4:301820/1‑149 (MQ=255)
                                                                                                                   tGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGAGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑AATCGAGATCGAGATGGAGCTGGGTACCGCAGGTACCGATGTTCGGGTGAAgt                         <  8:118647/149‑1 (MQ=255)
                                                                                                                        ttCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑AATCGAGATCGAGATGGAGCTGGGTACCGCAGGTACCGATGTTCGGGTGAAgtgtgt                     <  4:29878/148‑1 (MQ=255)
                                                                                                                         tCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑AATCGAGATCGAGATGGAGCTgg                                                       <  8:202429/113‑1 (MQ=255)
                                                                                                                         tCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑AATCGAGATCGAGATGGAGCTgg                                                       >  7:202429/1‑113 (MQ=255)
                                                                                                                         tCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑AATCGAGATCGAGATGGAGCTGGGTACCGCAGGTACCGATGTTCGGGTGAAGTGTGtt                    <  3:279701/148‑1 (MQ=255)
                                                                                                                              gCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑AATCGAGATCGAGATGGAGCTGGGTACCGCAGGTACCGATGTTCGGGTGAAGTGTGTTGACGCa              <  5:126452/149‑1 (MQ=255)
                                                                                                                                aTGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑AATCGAGATCGAGATGGAGCTGGGTACCGCAGGTACCGATGTTCGGGTGAAGTGTGTTGACGCACt            <  2:56362/149‑1 (MQ=255)
                                                                                                                                 tGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑AATCGAGATCGAGATGGAGCTGGGTACCGCAGGTACCGATGTTCGGGTGAAGTGTGTTGACGCACTg           <  3:301820/149‑1 (MQ=255)
                                                                                                                                     cgcgcTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑AATCGAGATCGAGATGGAGCTGGGTACCGCAGGTACCGATGTTCGGGTGAAGTGTGTTGACGCACTGGATg       <  4:45094/149‑1 (MQ=255)
                                                                                                                                      gcgcTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑AATCGAGATCGAGATGGAGCTGGGTACCGCAGGTACCGATGTTCgg                                >  4:124840/1‑123 (MQ=255)
                                                                                                                                      gcgcTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑AATCGAGATCGAGATGGAGCTGGGTACCGCAGGTACCGATGTTCgg                                <  3:124840/123‑1 (MQ=255)
                                                                                                                                          tGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑AATCGAGATCGAGATGGAGCTGGGTACCGCAGGTACCGATGTTCGGGTGAAGTGTGTTGACGCACTGGATGCACag  <  4:8448/149‑1 (MQ=255)
                                                                                                                                          tGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTAAACTCGTTTCGGACTTCAGCC‑AATCGAGATCGAGATGGAGCTGGGTACCGCAGGTACCGAAGTTCGGGTGAACTGTGTTGCCGCAATGGATGCACag  >  7:252136/1‑149 (MQ=255)
                                                                                                                                              cGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑AATCGAGATCGAGATGGAGCTGGGTACCGCAGGTACCGATGTTCGGGt                              <  6:73523/117‑1 (MQ=255)
                                                                                                                                              cGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑AATCGAGATCGAGATGGAGCTGGGTACCGCAGGTACCGATGTTCGGGt                              >  5:73523/1‑117 (MQ=255)
                                                                                                                                                    |                                                                                                                                           
AGGCGGATGAGATCCAGGGCATTCGTGCTTTCGGTGAGCAAACCGAACTGCAGGCAGTGCAGGACGTTGTGAACAAGCGTCTGGCGAAGGCTCGTCGCCAGTTGGACGCTACGCATGAGTTTCATCGCATGGGCGCGCTGAACGGCGTTGTTCTGGATGCGGACGGTAGCACCGTGCTGCTCAATATCTATGATCGTTTCGGACTTCAGCC‑AATCGAGATCGAGATGGAGCTGGGTACCGCAGGTACCGATGTTCGGGTGAAGTGTGTTGACGCACTGGATGCACAG  >  NC_002947/3431635‑3431921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑