Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,459,307 C→G 5.3% R352P (CGG→CCG)  PP_3075 ← transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,459,3070CG 5.3% 248.0 / 3.5 76R352P (CGG→CCG) PP_3075transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (38/34);  new base G (2/2);  total (40/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.51e-01

GCGCTCCAGCACATTGCGCAGCTCGCGAATGTTCCCCGGCCATGCATGCCTGGCCAGCAGTTGCTGCGCTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCGGGCCATGGCAGCCTGCAGGTCGATGGAGGTGGCGGCAATGATGCGCACATCGCTGCGCAGCATCTGGTTCGACCCGAC  >  NC_002947/3459159‑3459452
                                                                                                                                                    |                                                                                                                                                 
gcgcTCCAGCACATTGCGCAGCTCGCGAATGTTCCCCGGCCATGCATGCCTGGCCAGCAGTTGCTGCGCTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGcc                                                                                                                                                   <  8:51124/149‑1 (MQ=255)
    tCCAGCACATTGCGCAGCTCGCGAATGTTCCCCGGCCATGCATGCCTGGCCAGCAGTTGCTGCGCTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCaa                                                                                                                                               >  6:316848/1‑149 (MQ=255)
      cAGCACATTGCGCAGCTCGCGAATGTTCCCCGGCCATGCATGCCTGGCCAGCAGTTGCTGCGCTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCGGCAAcg                                                                                                                                             >  3:261350/1‑149 (MQ=255)
       aGCACATTGCGCAGCTCGCGAATGTTCCCCGGCCATGCATGCCTGGCCAGCAGTTGCTGCGCTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAAcgg                                                                                                                                            >  2:5671/1‑149 (MQ=255)
         cacaTTGCGCAGCTCGCGAATGTTCCCCGGCCATGCATGCCTGGCCAGCAGTTGCTGCGCTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAAcggcg                                                                                                                                          <  2:140/149‑1 (MQ=255)
          acaTTGCGCAGCTCGCGAATGTTCCCCGGCCATGCATGCCTGGCCAGCAGTTGCTGCGCTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCGGCAAcggctg                                                                                                                                         <  4:261350/149‑3 (MQ=255)
           caTTGCGCAGCTCGCGAATGTTCCCCGGCCATGCATGCCTGGCCAGCAGTTGCTGCGCTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAAcggcgg                                                                                                                                         >  5:168637/1‑148 (MQ=255)
            aTTGCGCAGCTCGCGAATGTTCCCCGGCCATGCATGCCTGGCCAGCAGTTGCTGCGCTTCAGCTTCCAGCTCGTACTGGCTG‑CCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGaa                                                                                                                                       >  3:227613/1‑148 (MQ=255)
                 gcAGCTCGCGAATGTTCCCCGGCCATGCATGCCTGGCCAGCAGTTGCTGCGCTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCCACGGCGGCACatc                                                                                                                                   >  5:30500/1‑148 (MQ=255)
                       cgcgAATGTTCCCCGGCCATGCATGCCTGGCCAGCAGTTGCTGCGCTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACat                                                                                                                                    <  4:241309/141‑1 (MQ=255)
                       cgcgAATGTTCCCCGGCCATGCATGCCTGGCCAGCAGTTGCTGCGCTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACat                                                                                                                                    >  3:241309/1‑141 (MQ=255)
                        gcgAATGTTCCCCGGCCATGCATGCCTGGCCAGCAGTTGCTGCGCTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCgg                                                                                                                             <  1:205783/147‑1 (MQ=255)
                         cgAATGTTCCCCGGCCATGCATGCCTGGCCAGCAGTTGCTGCGCTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGgcag                                                                                                                          >  6:145811/1‑149 (MQ=255)
                          gAATGTTCCCCGGCCATGCATGCCTGGCCAGCAGTTGCTGCGCTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGgcag                                                                                                                          >  7:264832/1‑148 (MQ=255)
                              gTTCCCCGGCCATCCATGCCTGCCCAGCAGTTGCTGCCCTTCAGCTTCCAGCTCGTACTCGCTGCCCACCTCACCCAATATGGCCTCGCACAGCGCCGGCACGACCTCCATCCCATGCCGCAACGGCGGAACATCGATCTCCCGCACAt                                                                                                                     >  8:191037/1‑149 (MQ=255)
                                 ccccGGCCATGCATGCCTGGCCAGCAGTTGCTGCGCTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACAtt                                                                                                                    >  5:109241/1‑147 (MQ=255)
                                  cccGGCCATGCATGCCTGGCCAGCAGTTGCTGCGCTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGCTCGGCAGCACATTCAg                                                                                                                 >  4:277264/1‑149 (MQ=255)
                                    cGGCCATGCATGCCTGGCCAGCAGTTGCTGCGCTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGcc                                                                                                               <  6:288632/149‑1 (MQ=255)
                                        catgcatgCCTGGCCAGCAGTTGCTGCGCTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGt                                                                                                            >  6:158289/1‑148 (MQ=255)
                                           gcatgcCTGGCCAGCAGTTGCTGCGCTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCg                                                                                                              <  2:344152/143‑1 (MQ=255)
                                           gcatgcCTGGCCAGCAGTTGCTGCGCTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCg                                                                                                              >  1:344152/1‑143 (MQ=255)
                                            catgcCTGGCCAGCAGTTGCTGCGCTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATa                                                                                                        <  5:316848/148‑1 (MQ=255)
                                            catgcCTGGCCAGCAGTTGCTGCGCTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATa                                                                                                        <  5:158289/148‑1 (MQ=255)
                                               gcCTGGCCAGCAGTTGCTGCGCTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAgg                                                                                                    >  2:49037/1‑149 (MQ=255)
                                                ccTGGCCAGCAGTTGCTGCGCTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAgg                                                                                                    <  1:382/148‑1 (MQ=255)
                                                    gCCAGCAGTTGCTGCGCTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGgc                                                                                               >  3:35259/1‑149 (MQ=255)
                                                     ccAGCAGTTGCTGCGCTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAAcggcgg                                                                                                                                         <  4:51123/106‑1 (MQ=255)
                                                     ccAGCAGTTGCTGCGCTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAAcggcgg                                                                                                                                         >  3:51123/1‑106 (MQ=255)
                                                      cagcagTTGCTGCGCTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGgcgc                                                                                             <  8:264832/149‑1 (MQ=255)
                                                       agcagTTGCTGCGCTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGcgcg                                                                                            <  5:145811/149‑1 (MQ=255)
                                                          agTTGCTGCGCTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGaa                                                                                         <  4:35259/149‑1 (MQ=255)
                                                            ttGCTGCGCTTCAGCTTCCAGCTCGTACTGGCTGCCCAGAGCATCCAATTTGGCCTCGCACAGCGCGTGCAGGGCCTCCAGCCGTTGCCGCAACGGCTGAACATCGATCGGCATCACATTCAGCCGGTAATACAGGTCGTCGCGGAAgt                                                                                       <  6:321460/149‑2 (MQ=255)
                                                             tgctgcGCTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACat                                                                                                                                    >  3:51551/1‑103 (MQ=255)
                                                             tgctgcGCTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACat                                                                                                                                    <  4:51551/103‑1 (MQ=255)
                                                                tgcGCTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGt                                                                                                            >  7:253215/1‑124 (MQ=255)
                                                                tgcGCTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGt                                                                                                            <  8:253215/124‑1 (MQ=255)
                                                                 gcgcTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCg                                                                                                 >  6:184208/1‑134 (MQ=255)
                                                                 gcgcTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCg                                                                                                 <  5:184208/134‑1 (MQ=255)
                                                                  cgcTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCcgc                                                                                 >  6:292239/1‑149 (MQ=255)
                                                                   gcTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCcgcg                                                                                >  5:271222/1‑149 (MQ=255)
                                                                       cAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCggg                                                                              <  6:109241/147‑1 (MQ=255)
                                                                        aGCTTCCAGCTCGTACTGGCTG‑CCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCGGGCCAt                                                                          <  4:227613/149‑1 (MQ=255)
                                                                           ttCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCGGGCCATgg                                                                        >  5:287410/1‑149 (MQ=255)
                                                                           ttCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCGGGCCATgg                                                                        >  7:323075/1‑149 (MQ=255)
                                                                             ccAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCGGGCCATgg                                                                        <  7:30533/147‑1 (MQ=255)
                                                                                  tCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCGGGCCATGGCAGCCTg                                                                 >  8:51349/1‑149 (MQ=255)
                                                                                    gTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGACGGCCCCGCGGGCCATGGCAGCCTGCa                                                               <  1:5671/149‑1 (MQ=255)
                                                                                    gTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCGGGCCATGGCAGCCTGCa                                                               <  1:49037/149‑1 (MQ=255)
                                                                                     tACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCGGGCCATGGCAGCCTGCAg                                                              >  4:336204/1‑149 (MQ=255)
                                                                                       cTGGCTGCCCAGGTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGGCGGTAATACAGGTCGGCGCGGATGGCCCCGCGGGCCATGGGAGCCTGCAgg                                                             <  7:191037/148‑1 (MQ=255)
                                                                                       cTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCGGGCCATGGCAGCCTGCAGGt                                                            <  3:336204/149‑1 (MQ=255)
                                                                                          gCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCGGGCCATGGCAGCCTGCAGGTCGa                                                         >  2:278371/1‑149 (MQ=255)
                                                                                              cccAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCGGGCCATGGCAGCCTGCAGGTCGATg                                                       <  7:51349/147‑1 (MQ=255)
                                                                                              cccAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCGGGCCATGGCAGCCTGCAGGTCGATg                                                       <  6:168637/147‑1 (MQ=255)
                                                                                                cAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCGGGCCATGGCAGCCTGCAGGTCGATGGAgg                                                   >  1:232327/1‑149 (MQ=255)
                                                                                                      aGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCGGGCCATGGCAGCCTGCAGGTCGATGGAGGTggcgg                                             >  3:8884/1‑149 (MQ=255)
                                                                                                       gCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCGGGCCATGGCAGCCTGCAGGTCGATGGAGGTggcgg                                             >  8:61393/1‑148 (MQ=255)
                                                                                                          aaTATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCGGGCCATGGCAGCCTGCAGGTCGATGGAGGTGGCGGCAat                                         >  2:272516/1‑149 (MQ=255)
                                                                                                          aaTATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCGGGCCATGGCAGCCTGCAGGTCGATGGAGGTGGCGGCAat                                         <  6:30500/149‑1 (MQ=255)
                                                                                                                gCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCGGGCCATGGCAGCCTGCAGGTCGATGGAGGTGGCGGCAATGATgc                                    <  3:87116/148‑1 (MQ=255)
                                                                                                                gCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCGGGCCATGGCAGCCTGCAGGTCGATGGAGGTGGCGGCAATGATgc                                    <  5:292239/148‑1 (MQ=255)
                                                                                                                 ccTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCGGGCCATGGCAGCCTGCAGGTCGATGGAGGTGGCGGCAATGATgcg                                   >  6:124762/1‑148 (MQ=255)
                                                                                                                   tCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCGGGCCATGGCAGCCTGCAGGTCGATGGAGGTGGCGGCAATGATGCGca                                 >  2:309669/1‑148 (MQ=255)
                                                                                                                     gCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCGGGCCATGGCAGCCTGCAGGTCGATGGAGGTGGCGGCAATGATGCGCACAt                              <  3:277264/149‑1 (MQ=255)
                                                                                                                               ggCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCGGGCCATGGCAGCCTGCAGGTCGATGGAGGTGGCGGCAATGATgcg                                   >  5:252272/1‑134 (MQ=255)
                                                                                                                               ggCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCGGGCCATGGCAGCCTGCAGGTCGATGGAGGTGGCGGCAATGATgcg                                   <  6:252272/134‑1 (MQ=255)
                                                                                                                                gCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCGGGCCATGGCAGCCTGCAGGTCGATGGAGGTGGCGGCAATGATGCGCACATCGCTGCgcag                    >  7:99496/1‑148 (MQ=255)
                                                                                                                                    gTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCGGGCCATGGCAGCCTGCAGGTCGATGGAGGTggcg                                              <  3:306065/118‑1 (MQ=255)
                                                                                                                                    gTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCGGGCCATGGCAGCCTGCAGGTCGATGGAGGTggcg                                              >  4:306065/1‑118 (MQ=255)
                                                                                                                                       ctccAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCGGGCCATGGCAGCCTGCAGGTCGATGGAGGTGGCGGCAATGATGCGCACATCGCTGCGCAGCATCTGGt            >  4:341911/1‑149 (MQ=255)
                                                                                                                                        tccAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCGGGCCATGGCAGCCTGCAGGTCGATGGAGGTGGCGGCAATGATGCGCACATCGCTGCGCAGCATCTGGtt           >  4:188972/1‑149 (MQ=255)
                                                                                                                                         tccgCCGTTGCGGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCGGGCCATGGCAGCCTGCAGGTCGATGGAGGTGGCGGCAATGATGCGCACATCGCTGCGCAGCATCTGGTTc          >  1:145418/4‑149 (MQ=255)
                                                                                                                                          ccgCCGTTGCGGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCGGGCCATGGCAGCCTGCAGGTCGATGGAGGTGGCGGCAATGATGCGCACATCGCTGCGCAGCATCTGGTTc          <  2:145418/146‑1 (MQ=255)
                                                                                                                                          cAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCGGGCCATGGCAGCCTGCAGGTCGATGGAGGTGGCGGCAATGATGCGCACATCGCTGCGCAGCATCTGGTTc          >  2:211557/1‑148 (MQ=255)
                                                                                                                                          cAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCGGGCCATGGCAGCCTGCAGGTCGATGGAGGTGGCGGCAATGATGCGCACATCGCTGCGCAGCATCTGGTTc          <  1:278371/148‑1 (MQ=255)
                                                                                                                                            gCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCGGGCCATGGCAGCCTGCAGGTCGATGGAGGTGGCGGCAATGATGCGCACATCGCTGCGCAGCATCTGGTTcgac       <  1:272516/149‑1 (MQ=255)
                                                                                                                                                  gCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCGGGCCATGGCAGCCTGCAGGTCGATGGAGGTGGCGGCAATGATGCGCACATCGCTGCGCAGCATCTGGTTcgacccgac  <  6:287410/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GCGCTCCAGCACATTGCGCAGCTCGCGAATGTTCCCCGGCCATGCATGCCTGGCCAGCAGTTGCTGCGCTTCAGCTTCCAGCTCGTACTGGCTGCCCAGCTCAGCCAATATGGCCTCGCACAGCGCGGGCAGGTCCTCCAGCCGTTGCCGCAACGGCGGAACATCGATCGGCAGCACATTCAGCCGGTAATACAGGTCGGCGCGGAAGGCCCCGCGGGCCATGGCAGCCTGCAGGTCGATGGAGGTGGCGGCAATGATGCGCACATCGCTGCGCAGCATCTGGTTCGACCCGAC  >  NC_002947/3459159‑3459452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑