Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,466,057	A→T	6.7%	noncoding (103/119 nt)	PP_mr39 ←	P15 ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,466,057	0	A	T	6.7%	170.6 / 2.7	60	noncoding (103/119 nt)	PP_mr39	P15 ncRNA
Reads supporting (aligned to +/- strand):  ref base A (29/27);  new base T (2/2);  total (31/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01

GAGGCGGCAGGCTGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAGCACAGGGCGCAG  >  NC_002947/3465913‑3466203                                                                                                                                                    |                                                                                                                                                   gAGGCGGCAGGCTGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGcc                                                                                                                                                 >  4:47777/1‑148 (MQ=255)       gcAGGCTGGCCCGAACTGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCggg                                                                                                                                          <  4:105296/149‑1 (MQ=255)       gcAGGCTGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCggg                                                                                                                                          <  2:139085/149‑1 (MQ=255)        cAGGCTGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCgggg                                                                                                                                         <  7:236304/149‑1 (MQ=255)        cAGGCTGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCgggg                                                                                                                                         <  8:297122/149‑1 (MQ=255)          ggCTGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGt                                                                                                                                       <  5:59903/149‑1 (MQ=255)            cTGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAACCAAATCC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGttt                                                                                                                                     <  4:235354/149‑1 (MQ=255)               gCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGa                                                                                                                                   <  3:305636/148‑1 (MQ=255)                   gAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGCTTCCCGACCGGGGGttttgttttt                                                                                                                              <  7:82375/149‑8 (MQ=38)                                 aaaTCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGCTTCCCGACCggggg                                                                                                                                        >  6:294334/1‑125 (MQ=255)                                 aaaTCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGGCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGCTTCCCGACCggggg                                                                                                                                        <  5:294334/125‑1 (MQ=255)                                   aTCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGcc                                                                                                              <  3:342504/149‑1 (MQ=255)                                      gCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCt                                                                                                           >  3:90249/1‑149 (MQ=255)                                           gCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGAGt                                                                                                                                 >  8:39292/1‑122 (MQ=255)                                           gCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGAGt                                                                                                                                 <  7:39292/122‑1 (MQ=255)                                                 gtgtTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGAc                                                                                                <  5:48566/149‑1 (MQ=255)                                                          tagtaATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTctgcgg                                                                                                                >  7:144751/1‑124 (MQ=255)                                                          tagtaATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTcggcgg                                                                                                                <  8:144751/124‑1 (MQ=255)                                                             taataaGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTATCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTa                                                                                    >  8:262079/1‑149 (MQ=255)                                                              aataaGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGCCCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTAt                                                                                   >  2:359027/1‑149 (MQ=255)                                                               ataaGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGa                                                                                                                                   >  6:117854/1‑100 (MQ=255)                                                               ataaGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGa                                                                                                                                   <  5:117854/100‑1 (MQ=255)                                                                  aGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGgc                                                                               <  1:359027/149‑1 (MQ=255)                                                                   gTGGCTTAGTTAATGAATCTGTCCTCAAATTGATTCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGgc                                                                               <  2:167809/148‑1 (MQ=255)                                                                   gTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACCTGGCCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCg                                                                                 >  1:112276/1‑149 (MQ=255)                                                                    tGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCGGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATc                                                                                  <  6:237013/144‑1 (MQ=255)                                                                    tGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATc                                                                                  >  5:237013/1‑144 (MQ=255)                                                                         tAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTa                                                                          <  4:239222/147‑1 (MQ=255)                                                                         tAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTa                                                                          >  3:239222/1‑147 (MQ=255)                                                                              cATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGTGCCTAGTGGCt                                                                    >  4:33738/1‑148 (MQ=255)                                                                               aTGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTa                                                                   >  6:221656/1‑148 (MQ=255)                                                                                 gAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAAc                                                                >  1:239802/1‑149 (MQ=255)                                                                                 gAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAAc                                                                >  6:91194/1‑149 (MQ=255)                                                                                  aaTCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAAc                                                                <  4:90249/148‑1 (MQ=255)                                                                                          cTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATa                                                           >  5:244989/1‑145 (MQ=255)                                                                                          cTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATa                                                           <  6:244989/145‑1 (MQ=255)                                                                                          cTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGCTCCGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGTCCCCTCGAAGCTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATAccc                                                        >  7:345127/1‑148 (MQ=255)                                                                                           tCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCa                                                       >  2:212342/1‑148 (MQ=255)                                                                                           tCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCa                                                       >  2:153598/1‑148 (MQ=255)                                                                                           tCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCAGCCCGGAGGTATGAGTAATCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAACCCAATACCCa                                                       >  5:251592/1‑148 (MQ=255)                                                                                            cAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCa                                                       >  1:272886/1‑147 (MQ=255)                                                                                                        gtgtTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCAAGTTAGGCGCCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGCCTAAACCAATACCCAAAGTAATAGCTGGc                                         >  7:184209/1‑149 (MQ=255)                                                                                                          gtTGGTCAAAGGGATGAAAAACAAAACC‑‑‑CCCGGTGTGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCTGCGGCCCCTCGTAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGTCTAAACCAATACCCAAAGTAATAGCTGGCa                                        <  8:345127/148‑1 (MQ=255)                                                                                                             ggTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAAc                                                                >  6:155825/1‑121 (MQ=255)                                                                                                             ggTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAAc                                                                <  5:155825/121‑1 (MQ=255)                                                                                                                 aaaGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCAc                                <  5:91194/149‑1 (MQ=255)                                                                                                                   aGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGgc                                                                               >  1:296882/1‑100 (MQ=255)                                                                                                                   aGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGgc                                                                               <  2:296882/100‑1 (MQ=255)                                                                                                                    ggCCTGAAAAACAACACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCa                             <  8:194605/149‑1 (MQ=255)                                                                                                                        tGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCg                         >  5:81079/1‑149 (MQ=255)                                                                                                                                   acC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAg              >  5:268221/1‑149 (MQ=255)                                                                                                                                     c‑‑‑cccGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAGca            >  6:122471/1‑149 (MQ=255)                                                                                                                                     c‑‑‑cccGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAGca            >  8:26831/1‑149 (MQ=255)                                                                                                                                     c‑‑‑cccGGTCGGCTTCCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAGca            >  8:259929/1‑149 (MQ=255)                                                                                                                                          ccGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAGcaca          <  6:81079/149‑1 (MQ=255)                                                                                                                                          ccGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAGca            >  1:157261/1‑147 (MQ=255)                                                                                                                                            ggTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAGCACAgg        <  1:212342/149‑1 (MQ=255)                                                                                                                                                 ggAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAGCACAGGGCGCa   <  3:47777/149‑1 (MQ=255)                                                                                                                                                  gAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAGCACAGGGCGCAg  <  2:272886/149‑1 (MQ=255)                                                                                                                                                  gAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAGCACAGGGCGCAg  >  8:320106/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                   GAGGCGGCAGGCTGGCCCGAACGGGATTGGCTAAATCGCCAGGCGTAGGTGTTTGCGTAGTAATAAGTGGCGTAGTTCATGAATCTGTCCTCAAAGTGAATCGGTGTTGGTCAAAGGCCTGAAAAACAAAACC‑‑‑CCCGGTCGGGAAGCCGACCGGGGGTTTGAGTATTCTCATGTTCGGCGGCCCCTCGAAGGTGGACGCCGGGTGGGTATCGGCGCCTAGTGGCTAAACCAATACCCAAAGTAATAGCTGGCAGGTGCCACGCAGCCGGCAACGGCCAGCACAGGGCGCAG  >  NC_002947/3465913‑3466203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑