| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,569,457 | A→T | 12.5% | intergenic (+16/+22) | PP_3150 → / ← sad‑II | hypothetical protein/NAD+‑dependent succinate semialdehyde dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,569,457 | 0 | A | T | 12.5% | 77.6 / 3.7 | 32 | intergenic (+16/+22) | PP_3150/sad‑II | hypothetical protein/NAD+‑dependent succinate semialdehyde dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (17/11); new base T (2/2); total (19/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01 | |||||||||||
AGGTGGCGGGTAACCGCAATGAACGGGCGCGTATGCTGGCGGGGTTGTATACCGCCTGTTCGCGGGCGCGGCATGAATTGATCGTGCCGGGGGGGATGCTGGACTGGGTCAAGGACCAGGTTCGGGATTGAAGCAGCTGGGGCCGCAAAGCAGCCCCAGGGTCATGAATCAGTTACGGTCCAGCCACACGGTCTGGGCATTGGTGAACTCGCGCACACCAAAGTGCGACAGCTCACGGCCGAAACCACTCTTCTTCACGCCACCAAACGCCACGCGGGGGTC > NC_002947/3569311‑3569592 | aGGTGGCGGGTAACCGCAATGAACGGGCGCGTATGCTGGCGGGGTTGTATACCGCCTGTTCGCGGGCGCGGCATGAATTGATCGTGCCGGGGGGGATGCTGGACTGGGTCAAGGACCAGGTTCGGGATTGAAGCAGCTGGGGCCGCaaa < 8:153940/149‑1 (MQ=255) aCGGGCGCTTATGCTGGCGGGGTTGTATACCGCCTGTTCGCGGGCGCGGCATGAATTGATCGTGCCGGGGGGGATGCTGGACTGGGTCAAGGACCAGGTTCGGGATTGAAGCAGCTGGGGCCGCAAAGCAGCCCCAGGGTCATGAATCa < 5:82029/149‑1 (MQ=255) cgTATGCTGGCGGGGTTGTATACCGACTGTTCGCGGGCGCGGCATGAATTGATCGTGCCGGGGGGGATGCTGGACTGGGTCAAGGACCAGGTTCGGGATTGAAGCAGCTGGGGCCGCAAAGCAGCCCCAGGGTCATGAATCAGTTACgg > 4:59007/1‑149 (MQ=255) ccGCCTGTTCGCGGGCGCGGCATGAATTGATCGTGCCGGGGGGGATGCTGGACTGGGTCAAGGACCAGGTTCGGGATTGAAGCAGCTGGGGCTGCTTTGCGGCCCCAgctgc > 1:223847/1‑108 (MQ=255) ccGCCTGTTCGCGGGCGCGGCATGAATTGATCGTGCCGGGGGGGATGCTGGACTGGGTCAAGGACCAGGTTCGGGATTGAAGCAGCTGGGGCTGCTTTGCGGCCCCAgctgc < 2:223847/112‑5 (MQ=255) cGCCTGTTCGCGGGCGCGGCATGAATTGATCGTGCCGGGGGGGATGCTGGACTGGGTCAAGGACCAGGTTCGGGATTGAAGCAGCTGGGGCCGCAAAGCAGcccc > 4:21919/1‑105 (MQ=255) cTGTTCGCGGGCGCGGCATGAATTGATCGTGCCGGGGGGGATGCTGGACTGGGTCAAGGACCAGGTTCGGGATTGAAGCAGCTGGGGCCGCAAAGCAGCCCCAGGGTCATGAATCAGTTACGGTCCAGCCACACGGTCTGGGCATTGGt < 7:96754/149‑1 (MQ=255) cTGTTCGCGGGCGCGGCATGAATTGATCGTGCCGGGGGGGATGCTGGACTGGGTCAAGGACCAGGATCGGGATTGAAGCAGCTGGGGCCGCAAAGCAGCCCCAGGGTCATGAATCAGTTACGGTCCAGCCACACGGTCTGGGCATTCGt > 4:288771/1‑149 (MQ=255) cgcgGGCGCGGCATGAATTGATCGTGCCGGGGGGGATGCTGGACTGGGTCAAGGACCAGGTTCGGGATTGAAGCAGCTGGGGCCGCAAAGCAGCCCCAGGGTCATGAATCAGTTACGGTCCAGCCACACGGTCTGGGCATTGGTGAAc < 8:110707/148‑1 (MQ=255) gAATTGATCGTGCCGGGGGGGATGCTGGACTGGGTCAAGGACCAGGTTCGGGATTGAAGCAGCTGGGGCCGCAAAGCAGCCCCAGGGTCATGAATCAGTTACGGTCCAGCCACACGGTCTGGGCATTGGTGAACTCGCGCACACCAAAg > 4:107315/1‑149 (MQ=255) aTTGATCGTGCCGGGGGGGATGCTGGACTGGGTCAAGGACCAGGTTCGGGATTGAAGCAGCTGGGGCCGCAAAGCAGCCCCAGGGTCATGAATCAGTTACGGTCCAGCCACACGGTCTGGGCATTGGTGAACTCGCGCACACCAAAGTg > 7:47706/1‑149 (MQ=255) cTGGACTGGGTCAAGGACCAGGTTCGGGATTGAAGCAGCTGGGGCCGCAAAGCAGCCCCAGGGTCATGAATCAGTTACGGTCCAGCCACACGGTCTGGGCATTGGTGAACTCGCGCACACCAAAGTGCGACAGCTCACGGCCGAAACCa < 3:59007/149‑1 (MQ=255) tGGACTGGGTCAAGGACCAGGTTCGGGATTGAAGCAGCTGGGGCCGCAAAGCAGCCCCAGGGTCATGAATCAGTTACGGTCCAGCCACACGGTCTGGGCATTGGTGAACTCGCGCACACCAAAGTGCGACAGCTCACGGCCGAAACCa > 1:322096/1‑148 (MQ=255) gACTGGGTCAAGGACCAGGTTCGGGATTGAAGCAGCTGGGGCCGCAAAGCAGCCCCAGGGTCATGAATCAGTTACGGTCCAGCCACACGGTCTGGGCATTGGTGAACTCGCGCACACCAAAGTGCGACAGCTCACGGCCGAAACCAct > 6:245432/1‑148 (MQ=255) ggTCAAGGACCAGGTTCGGGATTGAAGCAGCTGGGGCCGCAAAGCAGCCCCAGGGTCATGAATCAGTTACTGTCCAGCCACACGGTCTGGGCATTGGTGAACTCGCGCACACCAAAGTGCGACAGCTCACGGCCGAAACCACTcttctt < 8:47706/149‑1 (MQ=255) gTCAAGGACCAGGTTCGGGATTGAAGCAGCTGGGGCCGCAAAGCAGCCCCAGGGTCATGAATCAGTTACGGTCCAGCCACACGGTCTGGGCATTGGTGAACTCGCGCACACCAAAGTGCGACAGCTCACGGCCGAAACCACTcttctt > 1:254358/1‑148 (MQ=255) tCAAGGACCAGGTTCGGGATTGAAGCAGCTGGGGCCGCAAAGCAGCCCCAGGGTCATGAATCAGTTACGGTCCAGCCACACGGTCTGGGCATTGGTGAACTCGCGCACACCAAAGTGCGACAGCTCACGGCCGAAACCACTCTTCTTCa > 2:172256/1‑149 (MQ=255) gACCAGGTTCGGGATTGAAGCAGCTGGGGCCGCAAAGCAGCCCCAGGGTCATGAATCAGTTACGGTCCAGCCACACGGTCTGGGCATTGGTGAACTCGCGCACACCAAAGTGCGACAGCTCACGGCCGAAACCACTCTTCTTCACGcca > 6:151859/1‑149 (MQ=255) ccAGGTTCGGGATTGAAGCAGCTTGGGCCGCAAAGCAGCCCCAGGGTCATGAATCAGTTACGGTCCAGCCACACGGTCTGGGCATTGGTGAACTCGCGCACACCAAAGTGCGACAGCTCACGGCCGAAACCACTCTTCTTCACGccacc < 2:322096/149‑1 (MQ=255) ttCGGGATTGAAGCAGCTGGGGCCGCAAAGCAGCCCCAGGGTCATGAATCAGTTACGGTCCAGCCACACGGTCTGGGCATTGGTGAACTCGCGCACACCAAAGTGCGACAGCTCACGGCCGAAACCActct < 8:153684/131‑1 (MQ=255) ttCGGGATTGAAGCAGCTGGGGCCGCAAAGCAGCCCCAGGGTCATGAATCAGTTACGGTCCAGCCACACGGTCTGGGCATTGGTGAACTCGCGCACACCAAAGTGCGACAGCTCACGGCCGAAACCActct > 7:153684/1‑131 (MQ=255) tCGGGATTGAAGCAGCTGGGGCCGCAAAGCAGCCCCAGGGTCATGAATCAGTTACGGTCCAGCCACACGGTTTGGGCATTGGTGAACTCGCGCACACCAAAGTGCGACAGCTCACGGCCGAAACCACTCTTCTTCAc < 1:106680/137‑1 (MQ=255) tCGGGATTGAAGCAGCTGGGGCCGCAAAGCAGCCCCAGGGTCATGAATCAGTTACGGTCCAGCCACACGGACTGGGCATTGGTGAACTCGCGCACACCAAAGTGCGACAGCTCACGGCCGAAACCACTCTTCTTCAc > 2:106680/1‑137 (MQ=255) ttGAAGCAGCTGGGGCCGCAAAGCAGCCCCAGGGTCATGAATCAGTTACGGTCCAGCCACACGGTCTGGGCATTGGTGAACTCGCGCACACCAAAGTGCGACAGCTCACGGCCGAAAc > 5:353857/1‑118 (MQ=255) ttGAAGCAGCTGGGGCCGCAAAGCAGCCCCAGGGTCATGAATCAGTTACGGTCCAGCCACACGGTCTGGGCATTGGTGAACTCGCGCACACCAAAGTGCGACAGCTCACGGCCGAAAc < 6:353857/118‑1 (MQ=255) gAAGCAGCTGGGGCCGCAAAGCAGCCCCAGGGTCATGAATCAGTTACGGTCCAGCCACACGGTCTGGGCATTGGTGAACTCGCGCACACCAAAGTGCGACAGCTCACGGCCGAAACCACTCTTCTTCACGCCACCAAACGCCACGCgg < 5:245432/148‑1 (MQ=255) aaGCAGCTGGGGCCGCAAAGCAGCCCCAGGGTCATGAATCAGTTACGGTCCAGCCACACGGTCTGGGCATTGGTGAACTCGCGCACACCAAAGTGCGACAGCTCACGGCCGAAACCACTCTTCTTCACGCCCCCAAACGCCACGCgggg > 2:234681/1‑149 (MQ=255) aaGCAGCTGGGGCCGCAAAGCAGCCCCAGGGTCATGAATCAGTTACGGTCCAGCCACACGGTCTGGGCATTGGTGAACTCGCGCACACCAAAGTGCGACAGCTCACGGCCGAAACCACTCTTCTTCACGCCACCAAACGCCACGCgggg > 8:32385/1‑149 (MQ=255) aaGCAGCTGGGGCCGCAAAGCAGCCCCAGGGTCATGAATCAGTTACGGTCCAGCCACACGGTCTGGGCATTGGTGAACTCGCGCACACCAAAGTGCGACAGCTCACGGCCGAAACCACTCTTCTTCACGCCACCAAACGCCACGCggg < 3:107315/148‑1 (MQ=255) gcagcTGGGGCCGCAAAGTAGCCCCAGGGTCATGAATCAGTTACGGTCCAGCCACACGGTCTGGGCATTGGTGAACTCGCGCACACCAAAGTGCGACAGCTCACGGCCGAAACCACTCTTCTTCACGCCACCAAACGCCACGCGGGGGt > 6:253538/1‑149 (MQ=255) gcagcTGGGGCCGCAAAGCAGCCCCAGGGTCATGAATCAGTTACGGTCCAGCCACACGGTCTGGGCATTGGTGAACTCGCGCACACCAAAGTGCGACAGCTCACGGCCGAAACCACTCTTCTTCACGCCACCAAACGCCACGCGGGGGt > 2:114771/1‑149 (MQ=255) ccTGGGGCTGCTTTGCGGCCCCAGGGTCATGAATCAGTTACGGTCCAGCCACACGGTCTGGGCATTGGTGAACTCGCGCACACCAAAGTGCGACAGCTCACGGCCGAAACCACTCTTCTTCACGCCACCAAACGCCACGCGGGGGTc < 6:48457/146‑1 (MQ=255) ccTGGGGCTGCTTTGCGGCCCCAGGGTCATGAATCAGTTACGGTCCAGCCACACGGTCTGGGCATTGGTGAACTCGCGCACACCAAAGTGCGACAGCTCACGGCCGAAACCACTCTTCTTCACGCCACCAAACGCCACGCGGGGGTc > 5:48457/2‑147 (MQ=255) | AGGTGGCGGGTAACCGCAATGAACGGGCGCGTATGCTGGCGGGGTTGTATACCGCCTGTTCGCGGGCGCGGCATGAATTGATCGTGCCGGGGGGGATGCTGGACTGGGTCAAGGACCAGGTTCGGGATTGAAGCAGCTGGGGCCGCAAAGCAGCCCCAGGGTCATGAATCAGTTACGGTCCAGCCACACGGTCTGGGCATTGGTGAACTCGCGCACACCAAAGTGCGACAGCTCACGGCCGAAACCACTCTTCTTCACGCCACCAAACGCCACGCGGGGGTC > NC_002947/3569311‑3569592 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |