Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,620,295 T→G 6.3% S20R (AGC→CGC)  PP_3190 ← ornithine cyclodeaminase/mu‑crystallin family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,620,2950TG 6.3% 198.3 / 4.2 63S20R (AGC→CGC) PP_3190ornithine cyclodeaminase/mu‑crystallin family protein
Reads supporting (aligned to +/- strand):  ref base T (27/32);  new base G (2/2);  total (29/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

GACGGTGAAGGTCGGGCTGCCTTCGATGTAGGCCGACTTCACGCAACAGTCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACACAGGATCACGGCGACACGCTTGCCCTGATAGCGTTCGGCGAG  >  NC_002947/3620155‑3620438
                                                                                                                                            |                                                                                                                                               
gACGGTGAAGGTCGGGCTGCCTTCGATGTAGGCCGACTTCACGCAACAGTCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGcc                                                                                                                                          >  4:53812/1‑148 (MQ=255)
    gTGAAGGTCGGGCTGCCTTCGATGTAGGCCGACTTCACGCAACAGTCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCGGTCAGCCAACtg                                                                                                                                     <  3:215752/149‑2 (MQ=255)
       aaGGTCGGGCTGCCTTCGATGTAGGCCGACTTCACGCAACAGTCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGGAGCCTTCCTCCAGTTGGCTGACGGCCagggccgg                                                                                                                                  >  8:281607/1‑142 (MQ=255)
                   ccTTCGATGTAGGCCGACTTCACGCAACAGTCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATggg                                                                                                                       <  8:42391/148‑1 (MQ=255)
                   ccTTCGATGTAGGCCGACTTCACGCAACAGTCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATgg                                                                                                                        <  8:339882/147‑1 (MQ=255)
                   ccTTCGATGTAGGCCGACTTCACGCAACAGTCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGc                                                                                                                      <  7:300675/149‑1 (MQ=255)
                     ttCGATGTAGGCCGACTTCACGCAACAGTCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAAt                                                                                                                          <  7:179866/143‑1 (MQ=255)
                     ttCGATGTAGGCCGACTTCACGCAACAGTCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAAt                                                                                                                          >  8:179866/1‑143 (MQ=255)
                     ttCGATGTAGGCCGACTTCACGCAACAGTCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGcga                                                                                                                    >  2:121432/1‑149 (MQ=255)
                       cGATGTAGGCCGACTTCACGCAACAGTCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGcgac                                                                                                                   <  4:22171/148‑1 (MQ=255)
                             aGGCCGACTTCACGCAACAGTCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGcc                                                                                                                                          <  6:266783/119‑1 (MQ=255)
                              ggCCGACTTCACGCAACAGTCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCGGTCAGCCAACtggagga                                                                                                                                <  5:206736/128‑7 (MQ=255)
                              ggCCGACTTCACGCAACAGTCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCGGTCAGCCAACtggagga                                                                                                                                >  6:206736/1‑122 (MQ=255)
                                ccGACTTCACGCAACAGTCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGcc                                                                                                         <  1:121432/149‑1 (MQ=255)
                                        gcGCAACAGTCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTg                                                                                                   >  8:86146/2‑147 (MQ=255)
                                          gCAACAGTCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGa                                                                                               >  2:245561/1‑149 (MQ=255)
                                            aaCAGTCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGAcc                                                                                             >  7:277179/1‑149 (MQ=255)
                                             aCAGTCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGAccc                                                                                            <  7:86146/149‑1 (MQ=255)
                                             aCAGTCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGAccc                                                                                            <  5:177298/149‑1 (MQ=255)
                                             aCAGTCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGAccc                                                                                            <  2:128314/149‑1 (MQ=255)
                                                   aCCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCAtt                                                                                       <  1:245561/148‑1 (MQ=255)
                                                   aCCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCAtt                                                                                       >  5:217365/1‑148 (MQ=255)
                                                      gTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAAc                                                                                   <  3:53812/149‑1 (MQ=255)
                                                         cgcgCCCTTGAATGCGAATCCCTGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGTCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAACgg                                                                                 <  5:202482/148‑1 (MQ=255)
                                                          gcgcCCTTGAATGCGAAGCCCTGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAAc                                                                                   >  5:280513/1‑145 (MQ=255)
                                                          gcgcCCTTGAATGCGAAGCCCTGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAAc                                                                                   <  6:280513/145‑1 (MQ=255)
                                                          gcgcCCTTGAATGCGAAGCCCTGAACGGGCGGCACCTGCACCTTGCCTTCGTAATAGGCGATGAAGCCCTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTTCGCCTTGCTGAAGACCCAGATTGAACGGc                                                                                >  2:326477/1‑148 (MQ=255)
                                                           cgcCCTTGAATGCGAAGCCCTGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAAc                                                                                   >  6:309/1‑144 (MQ=255)
                                                           cgcCCTTGAATGCGAAGCCCTGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAAc                                                                                   <  5:309/144‑1 (MQ=255)
                                                              ccTTGAATGCGAAGCCCTGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAACGGCCTc                                                                             >  3:228697/1‑147 (MQ=255)
                                                                  gAATGCGAAGCCCTGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAACGGCCTCGAgg                                                                         <  4:228697/147‑1 (MQ=255)
                                                                        gAAGCCCTGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAACGGCCTCGAGGAACTTTTc                                                                 <  6:217365/149‑1 (MQ=255)
                                                                          aGCCCTGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAACGGCCTCGAGGAACTTTTCca                                                               <  8:277179/149‑1 (MQ=255)
                                                                          aGCCCTGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGACCTGCGCCTTGCTGAAGACCCTCATTGAACGGCCTCGAGGAACTTTTCca                                                               <  7:281607/149‑1 (MQ=255)
                                                                             ccTGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAACGGCCTCGAGGAACTTTTCcagca                                                            <  5:309252/149‑1 (MQ=255)
                                                                               tGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAACGGCCTCGAGGAACTTTTCcagcag                                                           >  7:237246/1‑148 (MQ=255)
                                                                                             ctgcacctTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCcaca                                            >  3:190239/1‑149 (MQ=255)
                                                                                                 acctTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACACTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACAAAg                                         >  7:137319/1‑148 (MQ=255)
                                                                                                  cctTGCCTTCGGAATAGGCGATTAATCCCTCCTCCAGTTGTCTGACCGCCAACTCCGGGTTGAAATGTGAGACGATCTGCGCCTTGCTGAAGACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGTCCACACAGGa                                       <  1:326477/149‑1 (MQ=255)
                                                                                                   ctTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACACAg                                         >  8:336144/1‑146 (MQ=255)
                                                                                                   ctTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACACAg                                         <  7:336144/146‑1 (MQ=255)
                                                                                                   ctTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACACAGGa                                       >  3:128634/1‑148 (MQ=255)
                                                                                                   ctTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACACAGGAt                                      >  5:58798/1‑149 (MQ=255)
                                                                                                   ctTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACACAGGAt                                      >  6:307129/1‑149 (MQ=255)
                                                                                                         ttCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACACAGGATCACGGc                                >  7:5541/1‑149 (MQ=255)
                                                                                                            ggAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAACGGCCTCGAGGAACTTTTACAGCAGGATGTTACGGCCACACAGGATCACGGCGac                             <  8:5541/149‑1 (MQ=255)
                                                                                                                    cGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCca                                              <  4:330652/124‑1 (MQ=255)
                                                                                                                     gATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACACAGGATCACGGCGACACGCTTGcc                    <  4:128634/149‑1 (MQ=255)
                                                                                                                     gATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACACAGGATCACGGCGACACGCTTGcc                    >  3:71299/1‑149 (MQ=255)
                                                                                                                        gAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACgg                                                 >  2:317414/1‑117 (MQ=255)
                                                                                                                        gAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACgg                                                 <  1:317414/117‑1 (MQ=255)
                                                                                                                             cTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACACAGGATCACGGCGac                             <  7:253216/132‑1 (MQ=255)
                                                                                                                             cTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACACAGGATCACGGCGac                             >  8:253216/1‑132 (MQ=255)
                                                                                                                              ttCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACACAGGATCACGGCGACACGCTTGCCCTGATAGCg           >  8:120706/1‑149 (MQ=255)
                                                                                                                               tcctccAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACACAGGATCACGGCGACACGCTTGc                     <  6:29191/138‑1 (MQ=255)
                                                                                                                               tcctccAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACACAGGATCACGGCGACACGCTTGc                     >  5:29191/1‑138 (MQ=255)
                                                                                                                                   ggaGTTGGCGGTCAGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAACGGCCTCGAGGAACTTTACCAGCAGGATGTTACGGCCACACAGGATCACGGCGACACGCTTGCCCTGATAGCGTACgg      >  2:83365/3‑149 (MQ=255)
                                                                                                                                   ccAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACACAGGATCACGGCGACACGCTTGCCCTGATAGCGTTCgg      >  8:91733/1‑149 (MQ=255)
                                                                                                                                    cAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACACAGGATCACGGCGACACGCTTGCCCTGATAGCGTTCgg      <  4:190239/148‑1 (MQ=255)
                                                                                                                                    cAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACACAGGATCACGGCGACACGCTTGCCCTGATAGCGTTCg       <  4:71299/147‑1 (MQ=255)
                                                                                                                                    cAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACACAGGATCACGGCGACACGCTTGCCCTGATAGCGTTCg       <  5:307129/147‑1 (MQ=255)
                                                                                                                                      gTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACACAGGATCACGGCGACACGCTTGCCCTGATAGCGTTCGGCGa   <  7:120706/149‑1 (MQ=255)
                                                                                                                                       ttGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACACAGGATCACGGCGACACGCTTGCCCTGATAGCGTTCGGCGAg  >  8:134730/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                               
GACGGTGAAGGTCGGGCTGCCTTCGATGTAGGCCGACTTCACGCAACAGTCACCGTTCGCGCCCTTGAATGCGAAGCCCTGCACGGGCGGCACCTGCACCTTGCCTTCGGAATAGGCGATGAAGCCTTCCTCCAGTTGGCTGACCGCCAACTCCGGGTTGAAATGGGCGACGATCTGCGCCTTGCTGAAGACCCTCATTGAACGGCCTCGAGGAACTTTTCCAGCAGGATGTTACGGCCACACAGGATCACGGCGACACGCTTGCCCTGATAGCGTTCGGCGAG  >  NC_002947/3620155‑3620438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑