Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,624,144	G→C	10.1%	intergenic (‑92/+81)	PP_3194 ← / ← PP_3195	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,624,144	0	G	C	10.1%	161.9 / 6.8	58	intergenic (‑92/+81)	PP_3194/PP_3195	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (31/21);  new base C (4/2);  total (35/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

GCCAGTGCCGTCGAACAGGGTGGAGCAGTGCAGGTGCAGGGCACTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCGGCGATCCAGTCGGCGATGTAGTGCCCGGCATTGATG  >  NC_002947/3624005‑3624288                                                                                                                                            |                                                                                                                                                  gCCAGTGCCGTCGAACAGGGTGGAGCAGTGCAGGTGCAGGGCACTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATAc                                                                                                                                         >  1:193129/1‑149 (MQ=255) gCCAGTGCCGTCGAACAGGGTGGAGCAGTGCAGGTGCAGGGCACTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATAc                                                                                                                                         >  5:145747/1‑149 (MQ=255)  ccAGTGCCGTCGAACAGGGTGGAGCAGTGCAGGTGCAGGGCACTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGCC‑ATCGATAcc                                                                                                                                        <  1:253302/149‑1 (MQ=255)  ccAGTGCCGTCGAACAGGGTGGAGCAGTGCAGGTGCAGGGCACTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGCC‑ATCGATAcc                                                                                                                                        <  5:314025/149‑1 (MQ=255)           tCGAACAGGGTGGAGCAGTGCAGGTGCAGGGCACTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGC‑At                                                                                                                                               >  5:366626/1‑133 (MQ=255)           tCGAACAGGGTGGAGCAGTGCAGGTGCAGGGCACTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGC‑At                                                                                                                                               <  6:366626/133‑1 (MQ=255)               aCAGGGTGGAGCAGTGCAGGTGCAGGGCACTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTgg                                                                                                                           >  4:341194/1‑149 (MQ=255)                   ggtctctcAGTTCAGGTGCAGGGCACTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTACCGATGGC‑ATCGATACCTGGCGCTCTCTGgc                                                                                                                          <  4:192519/139‑1 (MQ=255)                   ggTGGAGCAGTGCAGGTGCAGGGCACTGATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGgcgc                                                                                                                        <  2:187633/148‑1 (MQ=255)                   ggTGGAGCAGTGCAGGTGCAGGGCACTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGTCTGGCCGGCAGCCGCTGATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGgc                                                                                                                          >  3:192519/1‑146 (MQ=255)                   ggTGGAGCAGTGCAGGTGCAGGGCACTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGgcgc                                                                                                                        >  1:187633/1‑148 (MQ=255)                                aggtgcaggGCACTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACgg                                                                                                          >  1:99597/1‑149 (MQ=255)                                  gtgcaggGCACTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGgcg                                                                                                        >  2:67604/1‑149 (MQ=255)                                   tgcaggGCACTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGgcgc                                                                                                       >  1:3336/1‑149 (MQ=255)                                     caggGCACTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGcc                                                                                                      >  4:93354/1‑148 (MQ=255)                                        ggCACTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATc                                                                                                  >  8:172421/1‑149 (MQ=255)                                        ggCACTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGACCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATc                                                                                                  >  1:174932/1‑149 (MQ=255)                                          cACTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCgg                                                                                                <  1:67604/149‑1 (MQ=255)                                           aCTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGgc                                                                                               <  2:99597/149‑1 (MQ=255)                                           aCTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGgc                                                                                               <  2:193129/149‑1 (MQ=255)                                                    tGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCGATTGGcgccgttctc                                                                                      <  6:103163/149‑6 (MQ=255)                                                    tGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCaa                                                                                      <  7:172421/149‑1 (MQ=255)                                                           tctcATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAggg                                                                               >  4:222748/1‑149 (MQ=255)                                                               aTCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCgg                                                                           >  7:125216/1‑149 (MQ=255)                                                                            tgCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAg                                                              <  3:341194/149‑1 (MQ=255)                                                                             gCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATACATGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAgg                                                             >  6:280160/1‑149 (MQ=255)                                                                                    taggctGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGTCCGtgtg                                                      >  6:250770/1‑149 (MQ=255)                                                                                             gCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAg                                             >  4:308548/1‑149 (MQ=255)                                                                                              cGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAgg                                            <  3:308548/149‑1 (MQ=255)                                                                                               gACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGCGATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGcc                                                                    >  1:79566/1‑125 (MQ=255)                                                                                               gACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGgc                                                                                                         >  4:304936/1‑87 (MQ=255)                                                                                               gACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGgc                                                                                                         <  3:304936/87‑1 (MQ=255)                                                                                               gACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGcc                                                                    <  2:79566/124‑1 (MQ=255)                                                                                                     ccGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCgg                                     <  5:250770/149‑1 (MQ=255)                                                                                                       ggCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCgg                                     >  6:81535/1‑147 (MQ=255)                                                                                                       ggCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCgg                                     <  5:81535/147‑1 (MQ=255)                                                                                                       ggCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGA‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCGGc                                    >  3:49692/1‑148 (MQ=255)                                                                                                        gCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCGGCGa                                  <  5:280160/149‑1 (MQ=255)                                                                                                          aCCAGCAGATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCGGCGATc                                <  6:145747/149‑1 (MQ=255)                                                                                                            cagcagATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCGGCGATc                                >  4:356133/1‑147 (MQ=255)                                                                                                              gcagATGCTATTGATGGCCAGGTATCGATGGC‑ATCTATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAgg                                            <  7:53414/133‑1 (MQ=255)                                                                                                              gcagATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAgg                                            >  8:53414/1‑133 (MQ=255)                                                                                                              gcagATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCGGCGATCCAGt                            <  2:3336/149‑1 (MQ=255)                                                                                                               cagATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCGGCGATCCAGTc                           >  5:166463/1‑149 (MQ=255)                                                                                                                    gCTATTGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGc                                                                          >  3:180017/1‑97 (MQ=255)                                                                                                                        ttGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCGGCGATCCAGTCGGCGATGTa                  <  5:157189/149‑1 (MQ=255)                                                                                                                            tGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCGGCGATCCAGTCGGCGATGTAGTGc              >  8:26541/1‑149 (MQ=255)                                                                                                                             cgCCAGGTATCGATGCC‑ATCGATACCTGGTGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCGGCGATCCAGTCGGCGATGTAGTGcc             >  5:208897/2‑149 (MQ=255)                                                                                                                               ccAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGgcg                                                                                                        <  4:187832/56‑1 (MQ=255)                                                                                                                               ccAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGgcg                                                                                                        >  3:187832/1‑56 (MQ=255)                                                                                                                               ccAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCGGCGATCCAGTCGGCGATGTAGTGCCCg           >  2:99591/1‑149 (MQ=255)                                                                                                                                cAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGgccgcc                                                <  8:94454/111‑1 (MQ=255)                                                                                                                                cAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCGGCGATCCAGTCGGCGCTGTAGTGCCggg          >  8:298220/1‑149 (MQ=255)                                                                                                                                cAGGTATCGATGCC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCGGCGATCCAGTCGGCGATGTAGTGCCCgg          >  3:269981/1‑149 (MQ=255)                                                                                                                                cAGGTATCGATGCC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCGGCGATCCAGTCGGCGATGTAGTGCCCgg          >  1:353935/1‑149 (MQ=255)                                                                                                                                cAGGTATCGATGCC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCGGCGATCCAGTCGGCGATGTAGTGCCCgg          >  5:268826/1‑149 (MQ=255)                                                                                                                                   gTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCGGCGATCCAGTCGGCGATGTAGTGCCCGGCAt       >  4:197563/1‑149 (MQ=255)                                                                                                                                        gATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCGGCGATCCAGTCGGCGATGTAGTGCCCGGCATTGATg  >  5:290257/1‑149 (MQ=255)                                                                                                                                            |                                                                                                                                                  GCCAGTGCCGTCGAACAGGGTGGAGCAGTGCAGGTGCAGGGCACTCATTGGTGAGCTCTCTCATCTTGGGGGTGGTGCAAGGCTAGGCTGTGGCGACTGCCCGGCACCAGCAGATGCTATTGATGGCCAGGTATCGATGGC‑ATCGATACCTGGCGCTCTCTGGCGCAGAAACGAGAACGGCGCCAATCGGCGCCGCCCAAGCCAGGGCCGGCAGCGCCGATCAGGCCGTGTGGCCGCCAAGGGTTTCGGCGATCCAGTCGGCGATGTAGTGCCCGGCATTGATG  >  NC_002947/3624005‑3624288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑