Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,637,179 C→T 9.7% V369V (GTC→GTT)  PP_3203 → MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,637,1790CT 9.7% 111.8 / 2.3 41V369V (GTC→GTT) PP_3203MFS transporter
Reads supporting (aligned to +/- strand):  ref base C (18/19);  new base T (2/2);  total (20/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01

GCAGGTTGGCTTCGATCAGCGCGGCCGTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCACGATGAACGAAATTTTCCTTGGCGGGCGAGCCAATGCCAGTAGCTCTCTGCGCAGCGCCATGTGGCTGAC  >  NC_002947/3637038‑3637327
                                                                                                                                             |                                                                                                                                                    
gcagGTTGGATTCGATCAGCGCGGCCGTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCg                                                                                                                                               <  1:123277/149‑1 (MQ=255)
     ttGGCTTCGATAAGCGCGGCCGTGCCACGGGTGGGTTCACTTCGGCGATTTTAGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACaa                                                                                                                                          >  6:97102/1‑149 (MQ=255)
      tGGCTTCGATCAGCGCGGCCGTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAAc                                                                                                                                         >  7:265654/1‑149 (MQ=255)
       ggCTTCGATCAGCGCGGCCGTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAAct                                                                                                                                        >  8:52185/1‑149 (MQ=255)
               tCAGCGCGGCCGTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTgg                                                                                                                                >  3:40912/1‑149 (MQ=255)
                          gTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGc                                                                                                                     <  8:265654/149‑1 (MQ=255)
                          gTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGc                                                                                                                     <  3:42377/149‑1 (MQ=255)
                          gTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGc                                                                                                                     <  4:168933/149‑1 (MQ=255)
                                       gtTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTTGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATgc                                                                                                        >  2:167302/1‑149 (MQ=255)
                                       gtTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTTGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATgc                                                                                                        >  2:172767/1‑149 (MQ=255)
                                       gtTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAATCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATgc                                                                                                        <  5:141982/149‑1 (MQ=255)
                                        ttCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATgcg                                                                                                       >  7:292604/1‑149 (MQ=255)
                                             ttCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCAcc                                                                                                  >  4:319088/1‑149 (MQ=255)
                                                  cGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAActgctgct                                                                                                                                  >  1:58994/1‑112 (MQ=255)
                                                  cGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAActgctgct                                                                                                                                  <  2:58994/112‑1 (MQ=255)
                                                    aTTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGc                                                                                           <  2:208535/149‑1 (MQ=255)
                                                            gggTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACgg                                                                                   >  8:288783/1‑149 (MQ=255)
                                                             ggTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGt                                                                                  <  4:40912/149‑1 (MQ=255)
                                                                  gTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAg                                                                             >  7:187802/1‑149 (MQ=255)
                                                                     cGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGt                                                                           <  6:369034/148‑1 (MQ=255)
                                                                      gTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTg                                                                          <  8:187802/148‑1 (MQ=255)
                                                                        cAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTg                                                                                       >  6:326793/1‑133 (MQ=255)
                                                                           tCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCACGAt                                                                    <  5:97102/149‑1 (MQ=255)
                                                                              aTTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCACGATGaa                                                                 >  2:185522/1‑149 (MQ=255)
                                                                                  aTCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCACGATGAACGa                                                              >  5:67178/1‑148 (MQ=255)
                                                                                             aTTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAg                                                                             <  8:93377/122‑1 (MQ=255)
                                                                                              ttGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCACGATGAACGAAATTTTCCTTggc                                                 <  7:52185/149‑1 (MQ=255)
                                                                                                 aCCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCACGATGAACGAAATTTTcc                                                      >  2:28389/1‑141 (MQ=255)
                                                                                                 aCCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACCGTGGCAGGTGCACGATGAACGAAATTTTcc                                                      <  1:28389/141‑1 (MQ=255)
                                                                                                        gtgtGGCTACGCACCTGCCGCATGCACTGCTGCTGGTTGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCACGATGAACGAAATTTTCCTTGGCGGGCGAGc                                         <  1:172767/147‑1 (MQ=255)
                                                                                                        gtgtGGCTACGCACCTGCCGCATGCACTGCTGCTGGTTGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCACGATGAACGAAATTTTCCTTGGCGGGCGAGc                                         <  1:167302/147‑1 (MQ=255)
                                                                                                          gtgGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCACGATGAACGAAATTTTCCTTGGCGGGCGAGCCAAt                                     <  8:292604/149‑1 (MQ=255)
                                                                                                             gCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCACGATGAACGAAATTTTCCTTGGCGGGCGAGCCAATGc                                   <  3:319088/148‑1 (MQ=255)
                                                                                                                     ccTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCACGATGAACGAAATTTTCCTTGGCGGGCGAGCCAATGCCAGTAGctc                          >  2:35647/1‑149 (MQ=255)
                                                                                                                                 aCTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCACGATGAACGAAATTTTCCTTGGCGGGCGAGCCAATGc                                   >  5:364194/1‑128 (MQ=255)
                                                                                                                                 aCTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCACGATGAACGAAATTTTCCTTGGCGGGCGAGCCAATGc                                   <  6:364194/128‑1 (MQ=255)
                                                                                                                                     ctgctgGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCACGATGAACGAAATTTTCCTTGGCGGGCGAGCCAATGCCAGTAGCTCTCTGCGCAgc                >  4:132442/1‑143 (MQ=255)
                                                                                                                                     ctgctgGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCACGATGAACGAAATTTTCCTTGGCGGGCGAGCCAATGCCAGTAGCTCTCTGCGCAgc                <  3:132442/143‑1 (MQ=255)
                                                                                                                                     ctgctgGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCACGATGAACGAAATTTTCCTTGGCGGGCGAGCCAATGCCAGTAGCTCTCTGCGCAGCGCCAtg          >  3:291336/1‑149 (MQ=255)
                                                                                                                                        ctgGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCACGATGAACGAAATTTTCCTTGGCGGGCGAGCCAATGCCAGTAGCTCTCTGCGCAGCGCCAtgt         <  7:288783/147‑1 (MQ=255)
                                                                                                                                            tCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCACGATGAACGAAATTTTCCTTGGCGGGCGAGCCAATGCCAGTAGCTCTCTGCGCAGCGCCATGTGGCTGa   >  3:25109/1‑149 (MQ=255)
                                                                                                                                             cGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCACGATGAACGAAATTTTCCTTGGCGGGCGAGCCAATGCCAGTAGCTCTCTGCGCAGCGCCATGTGGCTGAc  >  7:37729/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
GCAGGTTGGCTTCGATCAGCGCGGCCGTGCCACGGGTGGGTTCACTTCGGCGATTTTCGCGGGTGAGTTCGTCAGTCCATTGATCGTCCTGGCATTGACCGCTGGTGTGGCTACGCACCTGCCGCATGCACTGCTGCTGGTCGCCATCGGACAACTGCTGCTGGCGCCTGTGTGCCTGTCCTTGATGCGCCACCGGCGTGCGGTGACGGTGGCAGGTGCACGATGAACGAAATTTTCCTTGGCGGGCGAGCCAATGCCAGTAGCTCTCTGCGCAGCGCCATGTGGCTGAC  >  NC_002947/3637038‑3637327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑