Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,655,760 C→T 7.1% L211L (CTG→CTA)  PP_3220 ← ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,655,7600CT 7.1% 163.2 / 2.1 56L211L (CTG→CTA) PP_3220ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (24/28);  new base T (2/2);  total (26/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01

TCGAGCAGGGCGCGGGTGTAGGGTTGCCGGGCGCCCCAGAGGATCTGCCGGGGCGAGCCGTGTTCGACCTGCTCACCCTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCGAGAATACGCCGCTGCACAGTCACGTCCAGCGCACTGGTGGGCTCGTCGGCGATGATCAACCGTGGCTCGCCGGCCAGGGCGATGGCAATCAGCACCCGCTG  >  NC_002947/3655620‑3655906
                                                                                                                                            |                                                                                                                                                  
tCGAGCAGGGCGCGGGTGTAGGGTTGCCGGGCGCCCCAGAGGATCTGCCGGGGCTAGCCGTGTTCGACCTGCTCACCCTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCga                                                                                                                                            <  1:177020/149‑1 (MQ=255)
tCGAGCAGGGCGCGGGTGTAGGGTTGCCGGGCGCCCCAGAGGATCTGCCGGGGCGAGCCGTGTTCGACCTGCTCACCCTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCga                                                                                                                                            <  8:102174/149‑1 (MQ=255)
  gAGCAGGGCGCGGGTGTAGGGTTGCCGGGCGCCCCAGAGGATCTGCCGGGGCGAGCCGTGTTCGACCTGCTCACCCTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCagcagc                                                                                                                                              >  4:121352/1‑145 (MQ=255)
    gCAGGGCGCGGGTGTAGGGTTGCCGGGCGCCCCAGAGGATCTGCCGGGGCGAGCCGTGTTCGACCTGCTCACCCTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGTCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATg                                                                                                                                        <  1:247566/149‑1 (MQ=255)
           gcgGGTGTAGGGTTGCCGGGCGCCCCAGAGGATCTGCCGGGGCGAGCCGTGTTCGACCTGCTCACCCTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCt                                                                                                                                 >  5:33086/1‑149 (MQ=255)
             gggTGTAGGGTTGCCGGGCGCCCCAGAGGATCTGCCGGGGCGAGCCGTGTTCGACCTGCTCACCCTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCg                                                                                                                               >  3:158718/1‑149 (MQ=255)
                            gggCGCCCCAGAGGATCTGCCGGGGCGAGCCGTGTTCGACCTGCTCACCCTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTg                                                                                                                 >  3:57304/1‑148 (MQ=255)
                             ggCGCCCCAGAGGATCTGCCGGGGCGAGCCGTGTTCGACCTGCTCACCCTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTg                                                                                                                 >  1:349024/1‑147 (MQ=255)
                               cgcCCCAGAGGATCTGCCGGGGCGAGCCGTGTTCGACCAGCTCACCCTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAgg                                                                                                             >  8:96202/1‑149 (MQ=255)
                                 ccccAGAGGATCTGCCGGGGCGAGCCGTGTTCGACCTGCTCACCCTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGt                                                                                                            >  5:87658/1‑148 (MQ=255)
                                  cccAGAGGATCTGCCGGGGCGAGCCGTGTTCGACCTGCTCACCCTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGa                                                                                                          >  3:74563/1‑149 (MQ=255)
                                     agagGATCTGCCGGGGCGAGCCGTGTTCGACCTGCTCACCCTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATTAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATc                                                                                                        >  4:159064/1‑148 (MQ=255)
                                     agagGATCTGCCGGGGCGAGCCGTGTTCGACCTGCTCACCCTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATTAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATc                                                                                                        <  3:153554/148‑1 (MQ=255)
                                     agagGATCTGCCGGGGCGAGCCGTGTTCGACCTGCTCACCCTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATTAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATc                                                                                                        <  3:159064/148‑1 (MQ=255)
                                     agagGATCTGCCGGGGCGAGCCGTGTTCGACCTGCTCACCCTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATTAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATc                                                                                                        >  4:153554/1‑148 (MQ=255)
                                        ggATCTGCCGGGGCGAGCCGTGTTCGACCTGCTCACCCTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGTCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCgaga                                                                                                    <  5:291171/149‑1 (MQ=255)
                                             tGCCGGGGCGAGCCGTGTTCGACCTGCTCACCCTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCGAGAATAcg                                                                                               >  3:146737/1‑149 (MQ=255)
                                               ccGGGGCGAGCCGTGTTCGACCTGCTCACCCTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGAc                                                                                                                          >  7:2548/1‑120 (MQ=255)
                                               ccGGGGCGAGCCGTGTTCGACCTGCTCACCCTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGAc                                                                                                                          <  8:2548/120‑1 (MQ=255)
                                               ccGGGGCGAGCCGTGTTCGACCTGCTCACCCTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCGAGAATAcgc                                                                                              <  6:87658/148‑1 (MQ=255)
                                                    gCGAGCCGTGTTCGACCTGCTCACCCTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCGAGAATACGCCGCTGc                                                                                        <  8:246269/149‑1 (MQ=255)
                                                      gAGCCGTGTTCGACCTGCTCACCCTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCGAGAATa                                                                                                 <  4:258368/138‑1 (MQ=255)
                                                      gAGCCGTGTTCGACCTGCTCACCCTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGAGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCGAGAATa                                                                                                 >  3:258368/1‑138 (MQ=255)
                                                          cGTGTTCGACCTGCTCACCCTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCGGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCGAGAATACGCCGCTGCACAGt                                                                                   >  5:74657/1‑148 (MQ=255)
                                                          cGTGTTCGACCTGCTCACCCTGCATCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCATCAGCGAGATGCCGCGCTCGGCGACGAGCAGTTGCAGGTGATCGAGAATACGCCGCTGCACAGTc                                                                                  <  4:51700/149‑1 (MQ=255)
                                                            tgtTCGACCTGCTCACCCTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCGAGAATACGCCGCTGCACAGTCAc                                                                                >  1:7686/1‑149 (MQ=255)
                                                              ttCGACCTGCTCACCCTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCGAGAATACGCCGCTGCACAGTCACGt                                                                              <  3:121352/149‑1 (MQ=255)
                                                               tCGACCTGCTCACCCTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCGAGAATACGCCGCTGCACAGTCACGt                                                                              <  7:203038/148‑1 (MQ=255)
                                                                 gACCTGCTCACCCTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCGAGAATACGCCGCTg                                                                                         >  8:270212/1‑135 (MQ=255)
                                                                 gACCTGCTCACCCTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCGAGAATACGCCGCTg                                                                                         <  7:270212/135‑1 (MQ=255)
                                                                 gACCTGCTCACCCTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCGAGAATACGCCGCTGCACAGTCACGTCCa                                                                           <  6:33086/149‑1 (MQ=255)
                                                                   ccTGCTCACCCTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGTTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCGAGAATACGCCGCTGCACAGTCACGTCCAgc                                                                         <  4:74563/149‑1 (MQ=255)
                                                                      gCTCACCCTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCGAGAATACGCCGCTGCACAGTCACGTCCAGCGCa                                                                      <  7:96202/149‑1 (MQ=255)
                                                                          aCCCTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCga                                                                                                      <  6:91230/113‑1 (MQ=255)
                                                                          aCCCTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCGAGAATACGCCGCTGCACAGTCACGTCCAGCGCACtgg                                                                  <  1:154967/149‑1 (MQ=255)
                                                                           cccTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCGAGAATACGCCGCTGCACAGTCACGTCCAGCGCACtggt                                                                 <  4:57304/149‑1 (MQ=255)
                                                                                cTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCGAGAATACGCCGCTGCACAGTCACGTCCAGCGCACTGGTGGGc                                                             <  2:7686/148‑1 (MQ=255)
                                                                                  tcatcaCCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCGAGAATAcg                                                                                               >  7:261793/1‑112 (MQ=255)
                                                                                  tcatcaCCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCGAGAATAcg                                                                                               <  8:261793/112‑1 (MQ=255)
                                                                                     tcaCCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCGAGAATACGCCGCTGCACAGTCACGTCCAGCGCACTGGTGGGCtcgtcg                                                       >  5:316323/1‑149 (MQ=255)
                                                                                              ggCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCGAGAATACGCCGCTGCACAGTCACGTCCAGCGCACTGGTGGGCTCGTCGGCgatgat                                               >  1:51323/1‑148 (MQ=255)
                                                                                                 ggTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCGAGAATACGCCGCTGCACAGTCACGTCCAGCGCACTGGTGGGCTCGTCGGCGATGATCAAc                                           <  1:142309/149‑1 (MQ=255)
                                                                                                   tCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCGAGAATACGCCGCTGCACAGTCACGTCCa                                                                           <  4:72681/115‑1 (MQ=255)
                                                                                                   tCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATAGAGAATACGCCGCTGCACAGTCACGTCCa                                                                           >  3:72681/1‑115 (MQ=255)
                                                                                                     ggCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCGAGAATACGCCGCTGCACAGTCACGTCCAGCGCACTGGTGGGCTCGTCGGCGATGATCAACCGt                                        <  6:316323/148‑1 (MQ=255)
                                                                                                     ggCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCGAGAATACGCCGCTGCACAGTCACGTCCAGCGCACTGGTGGGCTCGTCGGCGATGATCAACCGTg                                       <  2:51323/149‑1 (MQ=255)
                                                                                                       cgcgGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCGAGAATACGCCGCTGCACAGTCACGTCCAGCGCACTGGTGGGCTCGTCGGCGATGATCAACCGTgg                                      >  3:94246/1‑148 (MQ=255)
                                                                                                              acaGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCGAGAATACGCCGCTGCACAGTCACGTCCAGCGCACTGGTGGGCTCGTCGGCCATGATCAACCGTGGCTCGCCgg                              >  7:120358/1‑149 (MQ=255)
                                                                                                               caGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCGAGAATACGCCGCTGCACAGTCACGTCCAGCGCACTGGTGGGCTCGTCGGCGATGATCAACCGTGGCTCGCCGGc                             >  2:353432/1‑149 (MQ=255)
                                                                                                                aGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCGAGAATACGCCGCTGCACAGTCACGTCCAGCGCACTGGTGGGCTCGTCGGCGATGATCAACCGTGGCTCGCCGGc                             >  2:203363/1‑148 (MQ=255)
                                                                                                                 ggCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCGAGAATACGCCGCTGCACAGTCACGTCCAGCGCACTGGTGGGCTCGTCGGCGATGATCAACCGTGGCTCGCCGGCCa                           <  4:146737/149‑1 (MQ=255)
                                                                                                                      tGCCCAGATCATGGGTGATGGTCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCGAGAATACGCCGCTGCACAGTCACGTCCAGCGCACTGGTGGGCTCGTCGGCGATGATCAACCGTGGCTCGCCGGCCAGGGc                       >  4:68692/1‑148 (MQ=255)
                                                                                                                         ccAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCGAGAATACGCCGCTGCACAGTCACGTCCAGCGCACTGGTGGGCTCGTCGGCGATGATCAACCGTGGCTCGCCGGCCAGGGCGATg                   <  4:158718/149‑1 (MQ=255)
                                                                                                                                aTGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCGAGAATACGCCGCTGCACAGTCACGTCCAGCGCACTGGTGGGCTCGTCGGCGATGATCAACCGTGGCTCGCCGGCCAGGGCGATGGCAATc             <  2:216167/148‑1 (MQ=255)
                                                                                                                                aTGGGTGATGATCAGCAGCGAGATGCCGCGCACGGCGACCAGCAGTTGCAGGTGATCGAGAATACGCCGCTGCACAGTCACGTCCAGCGCACTGGTGGGCTCGTCGGCGATGATCAACCGTGGCTCGCCGGCCAGGGCGATGGCAATc             >  1:216167/1‑148 (MQ=255)
                                                                                                                                           tCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCGAGAATACGCCGCTGCACAGTCACGTCCAGCGCACTGGTGGGCTCGTCGGCGATGATCAACCGTGGCTCGCCGGCCAGGGCGATGGCAATCAGCACCCGCTg  <  4:94246/148‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                  
TCGAGCAGGGCGCGGGTGTAGGGTTGCCGGGCGCCCCAGAGGATCTGCCGGGGCGAGCCGTGTTCGACCTGCTCACCCTGCTTCATCACCAGCAGGCGGTCGGCGCGGTCACAGGCCATGCCCAGATCATGGGTGATGATCAGCAGCGAGATGCCGCGCTCGGCGACCAGCAGTTGCAGGTGATCGAGAATACGCCGCTGCACAGTCACGTCCAGCGCACTGGTGGGCTCGTCGGCGATGATCAACCGTGGCTCGCCGGCCAGGGCGATGGCAATCAGCACCCGCTG  >  NC_002947/3655620‑3655906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑