Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,718,102 A→C 9.3% L164R (CTG→CGG)  paaG ← 2‑(1,2‑epoxy‑1,2‑dihydrophenyl)acetyl‑CoA isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,718,1020AC 9.3% 159.0 / 8.0 54L164R (CTG→CGG) paaG2‑(1,2‑epoxy‑1,2‑dihydrophenyl)acetyl‑CoA isomerase
Reads supporting (aligned to +/- strand):  ref base A (24/25);  new base C (3/2);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.85e-01

CAGTGTCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCC  >  NC_002947/3717957‑3718233
                                                                                                                                                 |                                                                                                                                    
cAGTGTCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAgcg                                                                                                                                   >  4:248151/1‑149 (MQ=255)
     tCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCtt                                                                                                                               <  3:248151/148‑1 (MQ=255)
     tCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTg                                                                                                                              >  1:178772/1‑149 (MQ=255)
           cGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCgg                                                                                                                        >  1:129772/1‑149 (MQ=255)
           cGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCgg                                                                                                                        <  2:178772/149‑1 (MQ=255)
             tAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGc                                                                                                                      <  8:338290/149‑1 (MQ=255)
               ggTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGcc                                                                                                                     <  5:186059/148‑1 (MQ=255)
                   ggctgggcGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGc                                                                                                                 <  3:300963/148‑1 (MQ=255)
                           ggcgAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATg                                                                                                                  >  3:86416/1‑139 (MQ=255)
                           ggcgAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATg                                                                                                                  <  4:86416/139‑1 (MQ=255)
                              gAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGcgcg                                                                                                    <  4:12557/149‑1 (MQ=255)
                                 gTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGgca                                                                                                 <  3:358436/149‑1 (MQ=255)
                                   ggcgggcgAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGgcag                                                                                                >  6:33074/1‑148 (MQ=255)
                                    gcgggcgAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGgcagca                                                                                              <  2:156420/149‑1 (MQ=255)
                                         cgAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCaa                                                                                         <  5:348802/149‑1 (MQ=255)
                                                  gggCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCaa                                                                                         <  7:252020/140‑1 (MQ=255)
                                                  gggCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCaa                                                                                         >  8:252020/1‑140 (MQ=255)
                                                          tcaCGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCgg                                                                        >  1:225705/1‑149 (MQ=255)
                                                            aCGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCa                                                                      >  7:139069/1‑149 (MQ=255)
                                                              gCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCAcc                                                                    <  8:139069/149‑1 (MQ=255)
                                                                       catcatCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATc                                                           >  4:86227/1‑149 (MQ=255)
                                                                                ccacGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAg                                                  <  3:86227/149‑1 (MQ=255)
                                                                                      ggTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTgg                                            <  7:219866/149‑1 (MQ=255)
                                                                                        tAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGgc                                                                                                  >  3:258457/1‑93 (MQ=255)
                                                                                        tAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGgc                                                                                                  <  4:258457/93‑1 (MQ=255)
                                                                                           aTCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATc                                                           <  4:89219/129‑1 (MQ=255)
                                                                                           aTCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATc                                                           >  3:89219/1‑129 (MQ=255)
                                                                                                cccccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGAt                                          <  8:7910/141‑1 (MQ=255)
                                                                                                cccccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGAt                                          >  7:7910/1‑141 (MQ=255)
                                                                                                  cccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCAcc                                                                    >  1:162398/1‑113 (MQ=255)
                                                                                                  cccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCAcc                                                                    <  2:162398/113‑1 (MQ=255)
                                                                                                  cccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGcc                                <  5:33074/149‑1 (MQ=255)
                                                                                                      ttGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAgc                            <  2:196400/149‑1 (MQ=255)
                                                                                                              ccTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCgcccgc                          >  2:57710/1‑138 (MQ=255)
                                                                                                              ccTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCgcccgc                          <  1:57710/143‑6 (MQ=255)
                                                                                                              ccTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGggcggc                     >  8:14629/1‑148 (MQ=255)
                                                                                                                  ttCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCa                >  8:233741/1‑149 (MQ=255)
                                                                                                                   tCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCAc               >  8:63154/1‑149 (MQ=255)
                                                                                                                      gcgcCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAg            <  7:63154/149‑1 (MQ=255)
                                                                                                                          cAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCGCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAgcggg                         >  1:256843/1‑133 (MQ=255)
                                                                                                                          cAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAgcggg                         <  2:256843/132‑1 (MQ=255)
                                                                                                                          cAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCg        >  7:143387/1‑149 (MQ=255)
                                                                                                                           aaGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCCGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCg        >  2:281155/1‑148 (MQ=255)
                                                                                                                           aaGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCg        >  2:230564/1‑148 (MQ=255)
                                                                                                                                gcTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTg                                                        <  1:245476/95‑1 (MQ=255)
                                                                                                                                gcTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTg                                                        >  2:245476/1‑95 (MQ=255)
                                                                                                                                gcTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGCGCGGGCGGCCAGCACCAGGTCGCAGGc   >  5:285086/1‑148 (MQ=255)
                                                                                                                                gcTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGcc  <  7:233741/149‑1 (MQ=255)
                                                                                                                                gcTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGc   >  7:248961/1‑148 (MQ=255)
                                                                                                                                    ggtcggCATGGCCCGGGCCAAGGCGCTGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCa             <  1:311980/129‑1 (MQ=38)
                                                                                                                                    ggtcggCATGGCCCGGGCCAAGGCGCTGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCa             >  2:311980/6‑134 (MQ=38)
                                                                                                                                      tcggCATGGCCCGGGCCAAGGCGCTGGCCATGC‑TGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAg     >  2:321321/4‑140 (MQ=38)
                                                                                                                                         gCATGGCCCGGGCCAAGGCGCTGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCa             <  6:305389/129‑1 (MQ=255)
                                                                                                                                         gCATGGCCCGGGCCAAGGCGCTGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCa             >  5:305389/1‑129 (MQ=255)
                                                                                                                                                 |                                                                                                                                    
CAGTGTCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCC  >  NC_002947/3717957‑3718233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑