Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,718,108	T→A	9.4%	K162M (AAG→ATG) ‡	paaG ←	2‑(1,2‑epoxy‑1,2‑dihydrophenyl)acetyl‑CoA isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,718,108	0	T	A	9.4%	149.4 / 5.7	53	K162M (AAG→ATG) ‡	paaG	2‑(1,2‑epoxy‑1,2‑dihydrophenyl)acetyl‑CoA isomerase
Reads supporting (aligned to +/- strand):  ref base T (24/24);  new base A (3/2);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

TCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCG  >  NC_002947/3717962‑3718251                                                                                                                                                   |                                                                                                                                                 tCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCtt                                                                                                                                                 <  3:248151/148‑1 (MQ=255) tCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTg                                                                                                                                                >  1:178772/1‑149 (MQ=255)       cGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCgg                                                                                                                                          >  1:129772/1‑149 (MQ=255)       cGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCgg                                                                                                                                          <  2:178772/149‑1 (MQ=255)         tAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGc                                                                                                                                        <  8:338290/149‑1 (MQ=255)           ggTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGcc                                                                                                                                       <  5:186059/148‑1 (MQ=255)               ggctgggcGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGc                                                                                                                                   <  3:300963/148‑1 (MQ=255)                       ggcgAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATg                                                                                                                                    >  3:86416/1‑139 (MQ=255)                       ggcgAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATg                                                                                                                                    <  4:86416/139‑1 (MQ=255)                          gAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGcgcg                                                                                                                      <  4:12557/149‑1 (MQ=255)                             gTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGgca                                                                                                                   <  3:358436/149‑1 (MQ=255)                               ggcgggcgAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGgcag                                                                                                                  >  6:33074/1‑148 (MQ=255)                                gcgggcgAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGgcagca                                                                                                                <  2:156420/149‑1 (MQ=255)                                     cgAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCaa                                                                                                           <  5:348802/149‑1 (MQ=255)                                              gggCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCaa                                                                                                           <  7:252020/140‑1 (MQ=255)                                              gggCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCaa                                                                                                           >  8:252020/1‑140 (MQ=255)                                                      tcaCGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCgg                                                                                          >  1:225705/1‑149 (MQ=255)                                                        aCGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCa                                                                                        >  7:139069/1‑149 (MQ=255)                                                          gCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCAcc                                                                                      <  8:139069/149‑1 (MQ=255)                                                                   catcatCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATc                                                                             >  4:86227/1‑149 (MQ=255)                                                                            ccacGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAg                                                                    <  3:86227/149‑1 (MQ=255)                                                                                  ggTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTgg                                                              <  7:219866/149‑1 (MQ=255)                                                                                    tAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGgc                                                                                                                    >  3:258457/1‑93 (MQ=255)                                                                                    tAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGgc                                                                                                                    <  4:258457/93‑1 (MQ=255)                                                                                       aTCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATc                                                                             <  4:89219/129‑1 (MQ=255)                                                                                       aTCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATc                                                                             >  3:89219/1‑129 (MQ=255)                                                                                            cccccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGAt                                                            <  8:7910/141‑1 (MQ=255)                                                                                            cccccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGAt                                                            >  7:7910/1‑141 (MQ=255)                                                                                              cccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCAcc                                                                                      >  1:162398/1‑113 (MQ=255)                                                                                              cccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCAcc                                                                                      <  2:162398/113‑1 (MQ=255)                                                                                              cccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGcc                                                  <  5:33074/149‑1 (MQ=255)                                                                                                  ttGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAgc                                              <  2:196400/149‑1 (MQ=255)                                                                                                          ccTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCgcccgc                                            <  1:57710/143‑6 (MQ=255)                                                                                                          ccTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCgcccgc                                            >  2:57710/1‑138 (MQ=255)                                                                                                          ccTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGggcggc                                       >  8:14629/1‑148 (MQ=255)                                                                                                              ttCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCa                                  >  8:233741/1‑149 (MQ=255)                                                                                                               tCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCAc                                 >  8:63154/1‑149 (MQ=255)                                                                                                                  gcgcCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAg                              <  7:63154/149‑1 (MQ=255)                                                                                                                      cAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCGCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAgcggg                                           >  1:256843/1‑133 (MQ=255)                                                                                                                      cAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAgcggg                                           <  2:256843/132‑1 (MQ=255)                                                                                                                      cAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCg                          >  7:143387/1‑149 (MQ=255)                                                                                                                       aaGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCCGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCg                          >  2:281155/1‑148 (MQ=255)                                                                                                                       aaGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCg                          >  2:230564/1‑148 (MQ=255)                                                                                                                            gcTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTg                                                                          >  2:245476/1‑95 (MQ=255)                                                                                                                            gcTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTg                                                                          <  1:245476/95‑1 (MQ=255)                                                                                                                            gcTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGCGCGGGCGGCCAGCACCAGGTCGCAGGc                     >  5:285086/1‑148 (MQ=255)                                                                                                                            gcTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGcc                    <  7:233741/149‑1 (MQ=255)                                                                                                                            gcTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGc                     >  7:248961/1‑148 (MQ=255)                                                                                                                                ggtcggCATGGCCCGGGCCAAGGCGCTGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCa                               <  1:311980/129‑1 (MQ=38)                                                                                                                                ggtcggCATGGCCCGGGCCAAGGCGCTGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCa                               >  2:311980/6‑134 (MQ=38)                                                                                                                                  tcggCATGGCCCGGGCCAAGGCGCTGGCCATGC‑TGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAg                       >  2:321321/4‑140 (MQ=38)                                                                                                                                     gCATGGCCCGGGCCAAGGCGCTGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCa                               <  6:305389/129‑1 (MQ=255)                                                                                                                                     gCATGGCCCGGGCCAAGGCGCTGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCa                               >  5:305389/1‑129 (MQ=255)                                                                                                                                              gcgcCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTggcaccg  >  8:263524/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                 TCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCG  >  NC_002947/3717962‑3718251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑