Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,718,115	G→T	9.6%	R160R (CGG→AGG) ‡	paaG ←	2‑(1,2‑epoxy‑1,2‑dihydrophenyl)acetyl‑CoA isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,718,115	0	G	T	9.6%	160.2 / 7.2	52	R160R (CGG→AGG) ‡	paaG	2‑(1,2‑epoxy‑1,2‑dihydrophenyl)acetyl‑CoA isomerase
Reads supporting (aligned to +/- strand):  ref base G (24/23);  new base T (3/2);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.98e-01

CGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCC  >  NC_002947/3717968‑3718260                                                                                                                                                    |                                                                                                                                                   cGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCgg                                                                                                                                                   >  1:129772/1‑149 (MQ=255) cGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCgg                                                                                                                                                   <  2:178772/149‑1 (MQ=255)   tAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGc                                                                                                                                                 <  8:338290/149‑1 (MQ=255)     ggTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGcc                                                                                                                                                <  5:186059/148‑1 (MQ=255)         ggctgggcGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGc                                                                                                                                            <  3:300963/148‑1 (MQ=255)                 ggcgAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATg                                                                                                                                             >  3:86416/1‑139 (MQ=255)                 ggcgAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATg                                                                                                                                             <  4:86416/139‑1 (MQ=255)                    gAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGcgcg                                                                                                                               <  4:12557/149‑1 (MQ=255)                       gTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGgca                                                                                                                            <  3:358436/149‑1 (MQ=255)                         ggcgggcgAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGgcag                                                                                                                           >  6:33074/1‑148 (MQ=255)                          gcgggcgAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGgcagca                                                                                                                         <  2:156420/149‑1 (MQ=255)                               cgAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCaa                                                                                                                    <  5:348802/149‑1 (MQ=255)                                        gggCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCaa                                                                                                                    <  7:252020/140‑1 (MQ=255)                                        gggCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCaa                                                                                                                    >  8:252020/1‑140 (MQ=255)                                                tcaCGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCgg                                                                                                   >  1:225705/1‑149 (MQ=255)                                                  aCGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCa                                                                                                 >  7:139069/1‑149 (MQ=255)                                                    gCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCAcc                                                                                               <  8:139069/149‑1 (MQ=255)                                                             catcatCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATc                                                                                      >  4:86227/1‑149 (MQ=255)                                                                      ccacGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAg                                                                             <  3:86227/149‑1 (MQ=255)                                                                            ggTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTgg                                                                       <  7:219866/149‑1 (MQ=255)                                                                              tAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGgc                                                                                                                             >  3:258457/1‑93 (MQ=255)                                                                              tAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGgc                                                                                                                             <  4:258457/93‑1 (MQ=255)                                                                                 aTCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATc                                                                                      <  4:89219/129‑1 (MQ=255)                                                                                 aTCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATc                                                                                      >  3:89219/1‑129 (MQ=255)                                                                                      cccccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGAt                                                                     <  8:7910/141‑1 (MQ=255)                                                                                      cccccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGAt                                                                     >  7:7910/1‑141 (MQ=255)                                                                                        cccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCAcc                                                                                               >  1:162398/1‑113 (MQ=255)                                                                                        cccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCAcc                                                                                               <  2:162398/113‑1 (MQ=255)                                                                                        cccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGcc                                                           <  5:33074/149‑1 (MQ=255)                                                                                            ttGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAgc                                                       <  2:196400/149‑1 (MQ=255)                                                                                                    ccTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCgcccgc                                                     >  2:57710/1‑138 (MQ=255)                                                                                                    ccTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCgcccgc                                                     <  1:57710/143‑6 (MQ=255)                                                                                                    ccTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGggcggc                                                >  8:14629/1‑148 (MQ=255)                                                                                                        ttCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCa                                           >  8:233741/1‑149 (MQ=255)                                                                                                         tCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCAc                                          >  8:63154/1‑149 (MQ=255)                                                                                                            gcgcCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAg                                       <  7:63154/149‑1 (MQ=255)                                                                                                                cAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCGCGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAgcggg                                                    >  1:256843/1‑133 (MQ=255)                                                                                                                cAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAgcggg                                                    <  2:256843/132‑1 (MQ=255)                                                                                                                cAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCg                                   >  7:143387/1‑149 (MQ=255)                                                                                                                 aaGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCCGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCg                                   >  2:281155/1‑148 (MQ=255)                                                                                                                 aaGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCg                                   >  2:230564/1‑148 (MQ=255)                                                                                                                      gcTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTg                                                                                   <  1:245476/95‑1 (MQ=255)                                                                                                                      gcTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTg                                                                                   >  2:245476/1‑95 (MQ=255)                                                                                                                      gcTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGCGCGGGCGGCCAGCACCAGGTCGCAGGc                              >  5:285086/1‑148 (MQ=255)                                                                                                                      gcTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGcc                             <  7:233741/149‑1 (MQ=255)                                                                                                                      gcTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGc                              >  7:248961/1‑148 (MQ=255)                                                                                                                          ggtcggCATGGCCCGGGCCAAGGCGCTGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCa                                        <  1:311980/129‑1 (MQ=38)                                                                                                                          ggtcggCATGGCCCGGGCCAAGGCGCTGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCa                                        >  2:311980/6‑134 (MQ=38)                                                                                                                            tcggCATGGCCCGGGCCAAGGCGCTGGCCATGC‑TGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAg                                >  2:321321/4‑140 (MQ=38)                                                                                                                               gCATGGCCCGGGCCAAGGCGCTGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCa                                        <  6:305389/129‑1 (MQ=255)                                                                                                                               gCATGGCCCGGGCCAAGGCGCTGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCa                                        >  5:305389/1‑129 (MQ=255)                                                                                                                                        gcgcCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTggcaccg           >  8:263524/1‑149 (MQ=255)                                                                                                                                            catGGCCCGGGCCAAGG‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTggcaccggcac       >  4:255979/3‑149 (MQ=255)                                                                                                                                                 cccGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGgcc  >  6:331474/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                   CGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGC‑CGACCAAGCGCGGCAGCAGCCAAGTACCGCCGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTGGCACCGGCACCGGCC  >  NC_002947/3717968‑3718260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑