Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,740,687 C→T 6.2% T377M (ACG→ATG)  PP_3304 → Bcr/CflA family multidrug resistance transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,740,6870CT 6.2% 187.1 / 2.2 65T377M (ACG→ATG) PP_3304Bcr/CflA family multidrug resistance transporter
Reads supporting (aligned to +/- strand):  ref base C (31/29);  new base T (2/2);  total (33/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01

GCTTTTCCTATGGGGTATGGGTATTGCCAATCCGCTGGGAACGGCCTTGACCATGACACCGTTCGGGAAAGAAGCAGGACTGGCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGTGTGCTTTCTGGCAATGGTGCTTTT‑CGCTTGGTATGTCAAACGTAAGGCAATTGTCAGGTAGGAGTATCTCTGCATGAGGTTTGCTCCTGCTTCGCCTG  >  NC_002947/3740541‑3740833
                                                                                                                                                  |                                                                                                                                                    
gCTTTTCCTATGGGGTATGGGTATTGCCAATCCGCTGGGAACGGCCTTGACCATGACACCGTTCGGGAAAGAAGCAGGACTGGCATCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGc                                                                                                                                                    >  6:233115/1‑149 (MQ=255)
 cTTTTCCTATGGGGTATGGGTATTGCCAATCCGCTGGGAACGGCCTTGACCATGACACCGTTCGGGAAAGAAGCAGGACTGGCTTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGc                                                                                                                                                    <  5:233115/148‑1 (MQ=255)
       cTATGGGGTATGGGTATTGCCAATCCGCTGGGAACGGCCTTGACCATGACACCGTTCGGGAAAGAAGCAGGACTGGCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGtt                                                                                                                                             >  8:34363/1‑149 (MQ=255)
       cTATGGGGTATGGGTATTGCCAATCCGCTGGGAACGGCCTTGACCATGACACCGTTCGGGAAAGAAGCAGGACTGGCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGtt                                                                                                                                             >  1:330523/1‑149 (MQ=255)
        tATGGGGTATGGGTATTGCCAATCCGCTGGGAACGGCCTTGACCATGACACCGTTCGGGAAAGAAGCAGGACTGGCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTc                                                                                                                                            >  4:265780/1‑149 (MQ=255)
        tATGGGGTATGGGTATTGCCAATCCGCTGGGAACGGCCTTGACCATGACACCGTTCGGGAAAGAAGCAGGACTGGCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTc                                                                                                                                            >  2:345955/1‑149 (MQ=255)
         aTGGGGTATGGGTATTGCCAATCCGCTGGGAACGGCCTTGACCATGACACCGTTCGGGAAAGAAGCAGGACTGGCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTcc                                                                                                                                           >  7:318631/1‑149 (MQ=255)
          tGGGGTATGGGTATTGCCAATCCGCTGGGAACGGCCTTGACCATGACACCGTTCGGGAAAGAAGCAGGACTGGCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTccc                                                                                                                                          <  6:98016/149‑1 (MQ=255)
          tGGGGTATGGGTATTGCCAATCCGCTGGGAACGGCCTTGACCATGACACCGTTCGGGAAAGAAGCAGGACTGGCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTc                                                                                                                                            <  5:285123/147‑1 (MQ=255)
                  gggtatTGCCAATCCGCTGGGAACGGCCTTGACCATGACACCGTTCGGGAAAGAAGCAGGACTGGCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTg                                                                                                                                  >  1:337693/1‑149 (MQ=255)
                   ggtatTGCCAATCCGCTGGGAACGGCCTTGACCATGACACCGTTCGGGAAAGAAGCAGGACTGGCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGc                                                                                                                                 >  3:285746/1‑149 (MQ=255)
                          ccAATCCGCTGGGAACGGCCTTGACCATGACACCGTTCGGGAAAGAAGCAGGACTGGCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACt                                                                                                                           <  2:316206/148‑1 (MQ=255)
                           cAATCCGCTGGGAACGGCCTTGACCATGACACCGTTCGGGAAAGAAGCAGGACTGGCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTgg                                                                                                                         >  3:309016/1‑149 (MQ=255)
                              tCCGCTGGGAACGGCCTTGACCATGACACCGTTCGGGAAAGAAGCAGGACTGGCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTg                                                                                                                      >  8:188455/1‑149 (MQ=255)
                                            ccTTGACCATGACACCGTTCGGGAAAGAAGCAGGACTGGCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCg                                                                                                       <  1:345955/149‑1 (MQ=255)
                                            ccTTGACCATGACACCGTTCGGGAAAGAAGCAGGACTGGCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCg                                                                                                       <  2:330523/149‑1 (MQ=255)
                                              ttGACCATGACACCGTTCGGGAAAGAAGCAGGACTGGCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCAACTCAAA‑CCTCGgt                                                                                                     >  4:342637/1‑149 (MQ=255)
                                              ttGACAATGACACCGTTCGGGAAAGAAGCAGGACTGGCGTCAGCCCTTATGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGgt                                                                                                     >  2:236226/1‑149 (MQ=255)
                                                gACCATGACACCGTTCGGGAAAGAAGCAGGACTGGCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCATGCCg                                                                                                                                                  <  7:187033/103‑1 (MQ=255)
                                                gACCATGACACCGTTCGGGAAAGAAGCAGGACTGGCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCATGCCg                                                                                                                                                  >  8:187033/1‑103 (MQ=255)
                                                gACCATGACACCGTTCGGGAAAGAAGCAGGACTGGCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCATGCCg                                                                                                                                                  <  7:182004/103‑1 (MQ=255)
                                                gACCATGACACCGTTCGGGAAAGAAGCAGGACTGGCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCATGCCg                                                                                                                                                  >  8:182004/1‑103 (MQ=255)
                                                gACCATGACACCGTTCGGGAAAGAAGCAGGACTGGCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGgt                                                                                                     <  7:188455/147‑1 (MQ=255)
                                                gACCATGACACCGTTCGGGAAAGAAGCAGGACTGGCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGgt                                                                                                     <  7:34363/147‑1 (MQ=255)
                                                         acCGTTCGGGAAAGAAGCAGGACTGGCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGTGTGCTTTCTgg                                                                                          <  1:219027/149‑1 (MQ=255)
                                                         acCGTTCGGGAAAGAAGCAGGACTGGCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGTGTGCTTTCTgg                                                                                          >  1:28655/1‑149 (MQ=255)
                                                                gggAAAGAAGCAGGACTGGCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGTGTGCTTTCTGGCAATgg                                                                                    <  1:333246/148‑1 (MQ=255)
                                                                 ggAAAGAAGCAGGACTGGCGTCAGCCCTTTTGGGCTTCCTGACCATGGTGATCGGAGCAGTGTCGCCCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGTGTGCTTTCTGGCAATGGTg                                                                                  >  7:92223/1‑149 (MQ=255)
                                                                  gAAAGAAGCAGGACTGGCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGTGTGCTTTCTGGCAATGGTGc                                                                                 >  3:279243/1‑149 (MQ=255)
                                                                      gaagCAGGACTGGCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGTGTGCTTTCTGGCAATGGTGCtttt                                                                             <  2:337693/149‑1 (MQ=255)
                                                                         gCAGGACTGGCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGTGTGCTTTCTGGCAATGGTGCTTTT‑CGc                                                                         >  2:236505/1‑149 (MQ=255)
                                                                           aGGACTGGCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGTGTGCTTTCTGGCAATGGTGCTTTT‑CGCtt                                                                       <  8:318631/149‑1 (MQ=255)
                                                                            ggACTGGCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGTGTGCTTTCTGGCAATGGTGCTTTT‑CGCTTg                                                                      <  4:285746/149‑1 (MQ=255)
                                                                             gACTGGCGTCAGCCCTTTTGGGCTTGCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑GCTCGGTGTGCTTTCTGGCAATgtt                                                                                   <  1:182136/136‑3 (MQ=255)
                                                                             gACTGGCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAACCCTCGGTGTGCTTTCTGGCAATGGTGCTTTT‑CGCTTg                                                                      <  1:236505/149‑1 (MQ=255)
                                                                              aCTGGCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGTGTGCTTTCTGGCAATGGTGCTTTT‑CGCTTGGt                                                                    <  4:279243/149‑1 (MQ=255)
                                                                                  gCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGtgtg                                                                                                  <  5:173351/116‑1 (MQ=255)
                                                                                  gCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGtgtg                                                                                                  >  6:173351/1‑116 (MQ=255)
                                                                                  gCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGTGTGCTTTCTGGCAATg                                                                                     <  7:168328/129‑1 (MQ=255)
                                                                                  gCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGTGTGCTTTCTGGCAATGGTGCTTTT‑CGCTTGGTATg                                                                 >  3:143149/1‑148 (MQ=255)
                                                                                  gCGTCAGACCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGTGTGCTTTCTGGCAATg                                                                                     >  8:168328/1‑129 (MQ=255)
                                                                                     tCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGTGTGCTTTCTGGCAATGGTGCTTTT‑CGCTTGGTATGTCaa                                                             <  5:197743/149‑1 (MQ=255)
                                                                                        gCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGTGTGCTTTCTGGCAATGGTGCTTTT‑CGCTTGGTATGTCAAACg                                                          >  7:131400/1‑149 (MQ=255)
                                                                                            ttttGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGTGTGCTTTCTGGCAATGGTGCTTTT‑CGCTTGGTATGTCAAACGTAAg                                                      >  3:310159/1‑149 (MQ=255)
                                                                                            ttttGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGTGTGCTTTCTGGCAATGGTGCTTTT‑CGCTTGGTATGTCAAACGTAAg                                                      >  3:341957/1‑149 (MQ=255)
                                                                                            ttttGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGTGTGCTTTCTGGCAATGGTGCTTTT‑CGCTTGGTATGTCAAACGTAAg                                                      >  7:264265/1‑149 (MQ=255)
                                                                                                gggCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CATCGGTGGCATTACTGGCAATGGTGCTTTT‑CGATTGGTATGTAAAACTTAAGGCaa                                                  >  2:103938/1‑149 (MQ=255)
                                                                                                      ccTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGTGTGCTTTCTGGCAATGGTGCTTTT‑CGCTTGGTATGTCAAACGTAAGGCAATTGt                                              >  5:305575/1‑147 (MQ=255)
                                                                                                       cTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGTGTGCTTTCTGGCAATGGTGCTTTT‑CGCTTGGTATGTCAAACGTAAGGCAATTGTCa                                            <  4:341957/148‑1 (MQ=255)
                                                                                                       cTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGTGTGCTTTCTGGCAATGGTGCTTTT‑CGCTTGGTATGTCAAACGTAAGGCAATTGTCAg                                           >  4:179718/1‑149 (MQ=255)
                                                                                                         gACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTg                                                                                                                          <  5:333395/70‑1 (MQ=255)
                                                                                                         gACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTg                                                                                                                          >  6:333395/1‑70 (MQ=255)
                                                                                                           ccATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGTGTGCTTTCTGGCAATGGTGCTTTTCCGCTTGGTATGTCAAACGTAAGGCAATTGTCAGGt                                         <  1:236226/148‑1 (MQ=255)
                                                                                                             aTGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGTGTGCTTTCTGGCAATGGTGCTTTT‑CGCTTGGTATGTCAAACGTAAGGCAATTGTCAGGTAGGa                                     <  6:305575/149‑1 (MQ=255)
                                                                                                              tGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGTGTGCTTTCTGGCAATGGTGCTTTT‑CGCTTGGTATGTCAAACGTAAGGCAATTGTCAGGTAg                                       >  4:32459/1‑146 (MQ=255)
                                                                                                              tGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGTGTGCTTTCTGGCAATGGTGCTTTT‑CGCTTGGTATGTCAAACGTAAGGCAATTGTCAGGTAg                                       <  3:32459/146‑1 (MQ=255)
                                                                                                                gCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGTGTGCTTTCTGGCAATGGTGCTTTT‑CGCTTGGTATGTCAAACGTAAGGCAATTGTCAGGTAGGAGTa                                  >  8:7129/1‑149 (MQ=255)
                                                                                                                       gAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGTGTGCTTTCTGGCAATGGTGCTTTT‑CGCTTGGTATGTCAAACGTAAGGCAATTGTCAGGTAGGAGTATCTCTGc                           <  8:131400/149‑1 (MQ=255)
                                                                                                                                 gACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGTGTGCTTTCTGGCAATGGTGCTTTT‑CGCTTGGTATGTCAAACGTAAGGCAATTGTCAGGTAGGAGTAtctc                              >  7:52853/1‑136 (MQ=255)
                                                                                                                                 gACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGTGTGCTTTCTGGCAATGGTGCTTTT‑CGCTTGGTATGTCAAACGTAAGGCAATTGTCAGGTAGGAGTAtctc                              <  8:52853/136‑1 (MQ=255)
                                                                                                                                        aTGGTTTCCAGGCCGAAGGTCCCCTCGTTTTGCGTTCTGTGTGCGACTGAAA‑CCTTGGTGTGCTTTCTGGCAATGGTGGTTTT‑CGGTTGGTTTGTCATTGGTTTGGCAATTGTCAGGTAGGAGTATCTCTGCATTACGTTTGGTCCTg           <  8:264265/148‑1 (MQ=255)
                                                                                                                                             ttCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGTGTGCTTTCTGGCAATGGTGCTTTT‑CGCTTGGTATGTCAAACGTAAGGCAATTGTCAGGTAGGAGTATCTCTGCATGAGGTTTGCTCCt            >  8:206100/1‑142 (MQ=255)
                                                                                                                                             ttCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGTGTGCTTTCTGGCAATGGTGCTTTT‑CGCTTGGTATGTCAAACGTAAGGCAATTGTCAGGTAGGAGTATCTCTGCATGAGGTTTGCTCCt            <  7:206100/142‑1 (MQ=255)
                                                                                                                                               ccACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGTGTGCTTTCTGGCAATGGTGCTTTT‑CGCTTGGTATGTCAAACGTAAGGCAATTGTCAGGTAGGAGTATCTCTGCATGAGGTTTGCTCCTGCTTCGcc    <  4:309016/148‑1 (MQ=255)
                                                                                                                                                cACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGTGTGCTTTCTGGCAATGGTGCTTTT‑CGCTTGGTATGTCAAACGTAAGGCAATTGTCAGGTAGGAGTATCTCTGCATGAGGTTTGCTCCTGCTTCGCCTg  <  3:265780/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GCTTTTCCTATGGGGTATGGGTATTGCCAATCCGCTGGGAACGGCCTTGACCATGACACCGTTCGGGAAAGAAGCAGGACTGGCGTCAGCCCTTTTGGGCTTCCTGACCATGGCGATCGGAGCAGTGTCGACCTGGATGGTTTCCACGCCGAAGTTCCCCACGATTGCCGTACTGGGTGCCACTCAAA‑CCTCGGTGTGCTTTCTGGCAATGGTGCTTTT‑CGCTTGGTATGTCAAACGTAAGGCAATTGTCAGGTAGGAGTATCTCTGCATGAGGTTTGCTCCTGCTTCGCCTG  >  NC_002947/3740541‑3740833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑