Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,762,926	T→C	6.2%	F57L (TTC→CTC)	PP_3326 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,762,926	0	T	C	6.2%	236.5 / 4.6	80	F57L (TTC→CTC)	PP_3326	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (38/37);  new base C (2/3);  total (40/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01

GCTTCCCTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATC  >  NC_002947/3762779‑3763073                                                                                                                                                    |                                                                                                                                                     gCTTCCCTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGtt                                                                                                                                                     >  5:88997/1‑149 (MQ=255)  cTTCCCTGTTGGCCCTGATGGTCAGCGTCAACTCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTc                                                                                                                                                    <  2:206482/149‑1 (MQ=255)   ttCCCTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTc                                                                                                                                                    >  3:270825/1‑148 (MQ=255)   ttCCCTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGCTc                                                                                                                                                    >  3:212425/1‑148 (MQ=255)             gCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCgg                                                                                                                                        >  7:43684/1‑149 (MQ=255)             gCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCgg                                                                                                                                        >  6:16534/1‑149 (MQ=255)                    tGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACAt                                                                                                                                  >  8:333842/1‑148 (MQ=255)                      gTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGAt                                                                                                                               <  8:43684/149‑1 (MQ=255)                      gTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGAt                                                                                                                               >  7:65054/1‑149 (MQ=255)                            gTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGt                                                                                                                         >  4:355755/1‑149 (MQ=255)                            gTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGCTCGACCGTGACCGGGATCATGTTcc                                                                                                                             <  6:126660/146‑1 (MQ=255)                            gTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGCTCGACCGTGACCGGGATCATGTTcc                                                                                                                             >  5:126660/1‑146 (MQ=255)                              cAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCa                                                                                                                       >  5:128771/1‑149 (MQ=255)                               aaCGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCa                                                                                                                       <  7:1589/148‑1 (MQ=255)                               aaCGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGCTCGACCGTGACCGGGATCATGTTCCCGGTCa                                                                                                                       <  8:78694/149‑1 (MQ=39)                                aCGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACg                                                                                                                     >  7:36740/1‑149 (MQ=255)                                     ggCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGa                                                                                                                <  5:16534/149‑1 (MQ=255)                                      gcgcCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAg                                                                                                               >  7:261698/1‑149 (MQ=255)                                         cccACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAgcgc                                                                                                            <  8:9028/149‑1 (MQ=255)                                         cccACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGCTCGACCGTGACCGGGATCATGTTCCCGGTCAC‑GTCGAacgc                                                                                                            <  4:212425/149‑1 (MQ=38)                                           cACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCt                                                                                                          >  2:230262/1‑149 (MQ=255)                                            aCGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTg                                                                                                         <  4:270993/149‑1 (MQ=255)                                                   gtgGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAgcgc                                                                                                            <  7:166619/139‑1 (MQ=255)                                                   gtgGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAgcgc                                                                                                            >  8:166619/1‑139 (MQ=255)                                                       aCCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATg                                                                                               <  8:315065/148‑1 (MQ=255)                                                          gaggagCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGt                                                                                           <  7:333842/149‑1 (MQ=255)                                                             gagCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGtgct                                                                                        <  8:261698/149‑1 (MQ=255)                                                             gagCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGtgct                                                                                        <  6:88997/149‑1 (MQ=255)                                                              agCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGtgctg                                                                                       >  6:350636/1‑149 (MQ=255)                                                               gCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGtgctgc                                                                                      <  3:146243/149‑1 (MQ=255)                                                               gCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGtgctg                                                                                       >  7:164243/1‑148 (MQ=255)                                                                   cGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAAcc                                                                                  <  1:230262/149‑1 (MQ=255)                                                                    gTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCa                                                                                 <  4:270825/149‑1 (MQ=255)                                                                     tGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATccc                                                                                                                            <  5:228947/105‑1 (MQ=255)                                                                     tGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATccc                                                                                                                            >  6:228947/1‑105 (MQ=255)                                                                     tGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCAc                                                                                >  5:172757/1‑149 (MQ=255)                                                                     tGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCAc                                                                                <  6:128771/149‑1 (MQ=255)                                                                        cAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCTCGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTa                                                                             <  1:251514/149‑1 (MQ=255)                                                                        cAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTa                                                                             >  2:251514/1‑149 (MQ=255)                                                                                   ccGTGTCCGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCg                                                                  <  3:247615/149‑1 (MQ=255)                                                                                   ccGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCg                                                                  >  1:17125/1‑149 (MQ=255)                                                                                   ccGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCg                                                                  <  3:252416/149‑1 (MQ=255)                                                                                     gtgtACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCgg                                                                 >  1:335463/1‑148 (MQ=255)                                                                                           ggCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGc                                                          >  6:215382/1‑149 (MQ=255)                                                                                            gCCCCGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACCTGATCCCGGTCCCGGTCGCGCGCCTGGCGGCTCCTGCCGTGCTGCACCCACGTACCCCGCCTGCGGCACTGGcc                                                         >  1:346927/1‑149 (MQ=255)                                                                                            gCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTGACGGTCGAGCGCCTGGCGGCTCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGcc                                                         >  1:352499/1‑149 (MQ=255)                                                                                            gCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGcc                                                         >  1:27388/1‑149 (MQ=255)                                                                                             ccACGGCGCCGAGGATAACGCCTACAAGGCGTAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCg                                                        >  1:94868/1‑149 (MQ=255)                                                                                               aCGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGtg                                                      >  6:367235/1‑149 (MQ=255)                                                                                                  gcgcCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGtgctg                                                   <  2:335463/149‑1 (MQ=255)                                                                                                       gAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGa                                              >  1:23932/1‑149 (MQ=255)                                                                                                       gAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGa                                              >  8:207055/1‑149 (MQ=255)                                                                                                          gATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACaa                                           >  2:186290/1‑149 (MQ=255)                                                                                                             aaCGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACgg                                        >  3:119846/1‑149 (MQ=255)                                                                                                              aCGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACgg                                        <  2:17125/148‑1 (MQ=255)                                                                                                                gCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGcc                                     >  1:253165/1‑149 (MQ=255)                                                                                                                   tACAAGGCGGAAAAGATCAAGCGCGGCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCAGGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTg                                  <  8:164243/149‑1 (MQ=255)                                                                                                                    aCAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGtgct                                                    >  1:217820/1‑130 (MQ=255)                                                                                                                    aCAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGtgct                                                    <  2:217820/130‑1 (MQ=255)                                                                                                                     cAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTAcc                                                                           <  8:129110/106‑1 (MQ=255)                                                                                                                     cAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTAcc                                                                           >  7:129110/1‑106 (MQ=255)                                                                                                                     cAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTg                                  <  7:207055/147‑1 (MQ=255)                                                                                                                     cAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTg                                  <  5:350636/147‑1 (MQ=255)                                                                                                                      aaGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGt                                >  1:224235/1‑148 (MQ=255)                                                                                                                         gCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTcaca                            <  3:355755/149‑1 (MQ=255)                                                                                                                           ggAAAAGATAAAGCGCGCCTGGGCGTTCGA‑CCTGACCGGGAACATGATCCCGGTAACGGTCGACCGCCTGGCGGATCATGCCGTCCTGCAACCACATACCCCCCCTGCGGCACTTACCCTGCTGATCTACAACGGGCCTTCGTcacacc                          >  2:281075/1‑147 (MQ=255)                                                                                                                                  aTCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGtg                                                                                          <  3:183778/78‑1 (MQ=255)                                                                                                                                  aTCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCGGGCGGATCATGCCGtg                                                                                          >  4:183778/1‑78 (MQ=255)                                                                                                                                     aaGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCACCCCCTGACCCCGCCTGCGGCACTTGCCGTGCTGCGGGCCAACGGGCCTAGGTCACAGGGCCCGGAc                  >  3:216152/1‑147 (MQ=255)                                                                                                                                      aGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAg               <  5:215382/149‑1 (MQ=255)                                                                                                                                       gcgcgcCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAg               >  5:261570/1‑148 (MQ=255)                                                                                                                                          cgcCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAAt           <  2:23932/149‑1 (MQ=255)                                                                                                                                          cgcCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAAt           <  2:94868/149‑1 (MQ=255)                                                                                                                                            ccTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATgg         <  2:253165/149‑1 (MQ=255)                                                                                                                                             cTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGt                                >  4:125774/1‑125 (MQ=255)                                                                                                                                             cTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGt                                <  3:125774/125‑1 (MQ=255)                                                                                                                                             cTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATggg        >  5:53358/1‑149 (MQ=255)                                                                                                                                             cTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATggg        <  4:119846/149‑1 (MQ=255)                                                                                                                                                 gCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCaa    <  1:186290/149‑1 (MQ=255)                                                                                                                                                 gCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCaa    <  7:289333/149‑1 (MQ=255)                                                                                                                                                   gTTCGA‑CGCGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATc  <  2:75106/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     GCTTCCCTGTTGGCCCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATC  >  NC_002947/3762779‑3763073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑