Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,830,229 T→A 6.3% D384V (GAC→GTC)  PP_3383 ← gluconate 2‑dehydrogenase flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,830,2290TA 6.3% 184.1 / 3.0 63D384V (GAC→GTC) PP_3383gluconate 2‑dehydrogenase flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base T (28/31);  new base A (2/2);  total (30/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.60e-01

AGTAGTCGGCCGTGGCTTTCTTCCAGGCACTGCCCCAGGCCGGAGTGCCCGGCGGGTTGGAGGTACCGGCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAGTTGTTGGTGTAGGTGTCCTTGTCGAAGAACGCCTTCACTGTACCCATGTTCTGATAGGCGAAGTTACGCCCGACCACACCCTCGTTCTTTACCGGGTC  >  NC_002947/3830083‑3830377
                                                                                                                                                  |                                                                                                                                                     
agtagtCGGCCGTGGCTTTCTTCCAGGCACTGCCCCAGGCCGGAGTGCCCGGCGGGTTGGAGGTACCGGCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCg                                                                                                                                                     >  7:169975/1‑149 (MQ=255)
 gtagtCGGCCGTGGCTTTCTTCCAGGCACTGCCCCAGGCCGGAGTGCCCGGCGGGTTGGAGGTACCGGCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCgg                                                                                                                                                    >  4:274803/1‑149 (MQ=255)
 gtagtCGGCCGTGGCTTTCTTCCAGGCACTGCCCCAGGCCGGAGTGCCCGGCGGGTTGGAGGTACCGGCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCg                                                                                                                                                     <  6:274257/148‑1 (MQ=255)
 gtagtCGGCCGTGGCTTTCTTCCAGGCACTGCCCCAGGCCGGAGTGCCCGGCGGGTTGGAGGTACCGGCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCg                                                                                                                                                     <  2:68234/148‑1 (MQ=255)
    gtCGGCCGTGGCTTTCTTCCAGGCACTGCCCCAGGCCGGAGTGCCCGGCGGGTTGGAGGTACCGGCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGt                                                                                                                                                 >  1:202765/1‑149 (MQ=255)
    gtCGGCCGTGGCTTTCTTCCAGGCACTGCCCCAGGCCGGAGTGCCCGGCGGGTTGGAGGTACCGGCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGt                                                                                                                                                 >  8:196504/1‑149 (MQ=255)
               ccgccctcCAGGCACTGCCCCAGGCCGGAGTGCCCGGCGGGTTGGAGGTACCGGCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAAtcgt                                                                                                                                        <  1:305722/141‑1 (MQ=255)
                tttCTTCCAGGCACTGCCCCAGGCCGGAGTGCCCGGCGGGTTGGAGGTACCGGCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑a                                                                                                                                    >  1:161208/1‑149 (MQ=255)
                tttCTTCCAGGCACTGCCCCAGGCCGGAGTGCCCGGCGGGTTGGAGGTACCGGCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGACGGCGTTGAAATCGTCG‑a                                                                                                                                    >  7:289863/1‑149 (MQ=255)
                tttCTTCCAGGCACTGCCACAGGCCGGAGTGACCGGCGGGTTGGAGGTACCTGCAATCGGCCGGCTGCCGGACTCGTTCACCCACATTGGCGAACCGCCGACGAAGACGTGCGGGACATGGTCGAAGTTGTCGGCCTTGAAATCGTCG‑a                                                                                                                                    >  7:201944/1‑149 (MQ=255)
                  tcttcCAGGCACTGCCCCAGGCCGGAGTGCCCGGCGGGTTGGAGGTACCGGCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑a                                                                                                                                    <  5:32320/147‑1 (MQ=255)
                      ccAGGCACTGCCCCAGGCCGGAGTGCCCGGCGGGTTGGAGGTACCGGCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATgg                                                                                                                                 <  7:196504/146‑1 (MQ=255)
                       cAGGCACTGCCCCAGGCCGGAGTGCCCGGCGGGTTGGAGGTACCGGCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAAt                                                                                                                             >  7:266592/1‑149 (MQ=255)
                        aGGCACTGCCCCAGGCCGGAGTGCCCGGCGGGTTGGAGGTACCGGCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATg                                                                                                                            >  3:20766/1‑149 (MQ=255)
                         ggCACTGCCCCAGGCCGGAGTGCCCGGCGGGTTGGAGGTACCGGCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGc                                                                                                                           <  2:161208/149‑1 (MQ=255)
                           cACTGCCCCAGGCCGGAGTGCCCGGCGGGTTGGAGGTACCGGCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCa                                                                                                                         >  4:270371/1‑149 (MQ=255)
                           cACTGCCCCAGGCCAGAGTGCCCGGCGGGTTGGAGGTACCGGCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCa                                                                                                                         <  5:109874/149‑1 (MQ=255)
                                  ccAGGCCGGAGTGCCCGGCGGGTTGGAGGTACCGGCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGACGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCAc                                                                                                                  >  7:134284/1‑149 (MQ=255)
                                   cAGGCCGGAGTGCCCGGCGGGTTGGAGGTACCGGCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCAcc                                                                                                                 <  2:160235/149‑1 (MQ=255)
                                     ggCCGGAGTGCCCGGCGGGTTGGAGGTACCGGCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGACGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCcg                                                                                                               <  8:134284/149‑1 (MQ=255)
                                             tGCCCGGCGGGTTGGAGGTACCGGCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGAt                                                                                                        >  1:270097/1‑148 (MQ=255)
                                               cccGGCGGGTTGGAGGTACCGGCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGaa                                                                                                     >  4:181600/1‑149 (MQ=255)
                                                    cggGTTGGAGGTACCGGCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAgttgt                                                                                                <  2:202765/149‑1 (MQ=255)
                                                     gggTTGGAGGTACCGGCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAgttgtt                                                                                               <  8:266592/149‑1 (MQ=255)
                                                      ggTTTGAGGTACCTGCAATAGTCCGGGTGCCGGCCTGTTTCACCCAAATTGGCGAACCGCAGACGAAGCCGTGCTGGGCATGTTCGAAGTTTTCGGCTTTGAAATCGTCT‑ATGGCAATGCCATTGCCACCCGGGCCGATGAAGttgttg                                                                                              <  8:201944/149‑1 (MQ=255)
                                                      ggTTGGAGGTACCGGCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAGttgttg                                                                                              <  3:181600/149‑1 (MQ=255)
                                                          ggAGGTACCGGCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAGTTGTTGgtg                                                                                           <  2:270097/148‑1 (MQ=255)
                                                           gAGGTACCGGCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAAtcgtc                                                                                                                                       <  2:73350/104‑1 (MQ=255)
                                                           gAGGTACCGGCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAAtcgtc                                                                                                                                       >  1:73350/1‑104 (MQ=255)
                                                           gAGGTACCGGCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGACGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAGTTGTTGGTGTa                                                                                         <  8:289863/149‑1 (MQ=255)
                                                            aGGTACCGGCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAGTTGTTGGTGTAg                                                                                        <  4:158469/149‑1 (MQ=255)
                                                            aGGTACCGGCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAGTTGTTGGTGTAg                                                                                        <  4:219960/149‑1 (MQ=255)
                                                              gTACCGGCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAGTTGTTGGTGTAGgt                                                                                      >  4:268427/1‑149 (MQ=255)
                                                                 ccGGCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAGTTGTTGGTGTAGgtgt                                                                                    <  3:268427/148‑1 (MQ=255)
                                                                  cGGCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAGttgttg                                                                                              >  5:24856/1‑137 (MQ=255)
                                                                  cGGCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAGttgttg                                                                                              <  6:24856/137‑1 (MQ=255)
                                                                   ggCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAgttgtt                                                                                               <  6:60816/135‑1 (MQ=255)
                                                                   ggCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAgttgtt                                                                                               >  5:60816/1‑135 (MQ=255)
                                                                          ggccggcTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAGTTGTTGGTGTAGGTGTCCTTGTCgaa                                                                          <  3:270371/149‑1 (MQ=255)
                                                                            ccggcTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAGTTGTTGGTGTAGGTGTCCTTGTCgaaga                                                                        <  5:87814/149‑1 (MQ=255)
                                                                            ccggcTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAGTTGTTGGTGTAGGTGTCCTTGTCgaag                                                                         >  8:59754/1‑148 (MQ=255)
                                                                              ggcTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAGTTGTTGGTGTAGGTGTCCTTGTCGAAGAAc                                                                      >  8:296700/1‑149 (MQ=255)
                                                                              ggcTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAGTTGTTGGTGTAGGTGTCCTTGTCGAAGAAc                                                                      <  7:296700/149‑1 (MQ=255)
                                                                                   ccGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAGTTGTTgg                                                                                             <  4:321180/121‑1 (MQ=255)
                                                                                   ccGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAGTTGTTgg                                                                                             >  3:321180/1‑121 (MQ=255)
                                                                                           gTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAGTTGTTGGTGTAGGTGTCCTTGTCGAAGAACGcc                                                                   <  2:81958/139‑1 (MQ=255)
                                                                                           gTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAGTTGTTGGTGTAGGTGTCCTTGTCGAAGAACGcc                                                                   >  1:81958/1‑139 (MQ=255)
                                                                                           gTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAGTTGTTGGTGTAGGTGTCCTTGTCGAAGAACGCCTTCACTGTAc                                                         >  2:45114/1‑149 (MQ=255)
                                                                                               aCCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAGTTGTTGGTGTAGGTGTCCTTGTCGAAGAACGc                                                                    <  4:153442/134‑1 (MQ=255)
                                                                                               aCCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAGTTGTTGGTGTAGGTGTCCTTGTCGAAGAACGc                                                                    >  3:153442/1‑134 (MQ=255)
                                                                                                 ccACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAGTTGTTGGTGTAGGTGTCCTTGTCGAAGAACGCCTTCACTGTACCCATGt                                                   >  2:137283/1‑149 (MQ=255)
                                                                                                 ccACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAGTTGTTGGTGTAGGTGTCCTTGTCGAAGAACGCCTTCACTGTACCCATGt                                                   >  5:261289/1‑149 (MQ=255)
                                                                                                  cacaTTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAGTTGTTGGTGTAGGTGTCCTTGTCGAAGAACGCCTTCACTGTACCCATGtt                                                  >  7:162757/1‑149 (MQ=255)
                                                                                                      ttGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCACTTCCATTGCCACCCGCGCCGATGAAGTTGTTGGTGTAGGTGTCCTTGTCGAAGAACGCCTTCACTGTACCCATGTTCTGa                                              <  7:230102/149‑1 (MQ=255)
                                                                                                        ggCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAGTTGTTGGTGTAGGTGTCCTTGTCGAAGAACGCCTTCACTGTACCCATGTTCTGAt                                             <  3:274803/148‑1 (MQ=255)
                                                                                                           gAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAGTTGTTGGTGTAGGTGTCCTTGTCGAAGAACGCCTTCACTGTACCCATGTTCTGATAGGc                                         <  6:261289/149‑1 (MQ=255)
                                                                                                              ccgccgACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAGTTGTTGGTGTAGGTGTCCTTGTCGAAGAACGCCTTCACTGTACCCATGTTCTGATAGGCGaa                                      <  4:20766/149‑1 (MQ=255)
                                                                                                              ccgccgACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAGTTGTTGGTGTAGGTGTCCTTGTCGAAGAACGCCTTCACTGTACCCATGTTCTGATAGGCGaa                                      <  8:169975/149‑1 (MQ=255)
                                                                                                                    acgaAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAGTTGTTGGTGTAGGTGTCCTTGTCGAAGAACGCCTTCACTGTACCCAAGTTCTGATAGGCGAAGTTACg                                >  6:372571/1‑149 (MQ=255)
                                                                                                                     cgaAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAGTTGTTGGTGTAGGTGTCCTTGTCGAAGAACGCCTTCACTGTACCCATGTTCTGATAGGCGAAGTTACGc                               <  8:162757/149‑1 (MQ=255)
                                                                                                                      gaAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCGAATGGCAATGCCATTGCCACCCGCGCCGATGAAGTTGTTGGTGTAGGTGTCCTTGTCGAAGAACGCCTTCACTGt                                                           >  1:78207/1‑121 (MQ=255)
                                                                                                                        aGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAGTTGTTGGTGTAGGTGTCCTTGTCGAAGAACGCCTTCACTGTACCCATGTTCTGATAGGCGAAGTTACGccc                             >  2:304900/1‑148 (MQ=255)
                                                                                                                                           gAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAGTTGTTGGTGTAGGTGTCCTTGTCGAAGAACGCCTTCACTGTACCCATGTTCTGATAGGCGAAGTTACGCCCGACCACACCCTCGTTCTTTa         >  1:5624/1‑149 (MQ=255)
                                                                                                                                                  tCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAGTTGTTGGTGTAGGTGTCCTTGTCGAAGAACGCCTTCACTGTACCCATGTTCTGATAGGCGAAGTTACGCCCGACCACACCCTCGTTCTTTACCGGGTc  >  2:182877/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
AGTAGTCGGCCGTGGCTTTCTTCCAGGCACTGCCCCAGGCCGGAGTGCCCGGCGGGTTGGAGGTACCGGCAATCGGCCGGCTGCCGGCCTGGTTCACCCACATTGGCGAACCGCCGACGAAGCCGTGCGGGCCATGGTCGAAGTTGTCGGCGTTGAAATCGTCG‑ATGGCAATGCCATTGCCACCCGCGCCGATGAAGTTGTTGGTGTAGGTGTCCTTGTCGAAGAACGCCTTCACTGTACCCATGTTCTGATAGGCGAAGTTACGCCCGACCACACCCTCGTTCTTTACCGGGTC  >  NC_002947/3830083‑3830377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑