Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,863,840 A→G 5.4% P921P (CCA→CCG)  PP_3413 → two‑component system sensor histidine kinase/response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,863,8400AG 5.4% 227.0 / 2.1 74P921P (CCA→CCG) PP_3413two‑component system sensor histidine kinase/response regulator
Reads supporting (aligned to +/- strand):  ref base A (35/35);  new base G (2/2);  total (37/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.73e-01

GACACTGGCGGAGAATGGCGAGATCGCCCTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCACCTGCCCCAGGTGCGCCCTCAGCAGC  >  NC_002947/3863693‑3863985
                                                                                                                                                   |                                                                                                                                                  
gACACTGGCGGAGAATGGCGAGATCGCCCTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGACCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCca                                                                                                                                                    <  7:211901/148‑1 (MQ=255)
   acTGGCGGAGAATGGCGAGATCGCCCTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCcagcag                                                                                                                                                <  8:290582/149‑1 (MQ=255)
    cTGGCGGAGAATGGCGAGATCGCCCTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAgg                                                                                                                                               <  5:356130/149‑1 (MQ=255)
     tGGCGGAGAATGGCGAGATCGCCCTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGc                                                                                                                                              >  8:240588/1‑149 (MQ=255)
       gCGGAGAATGGCGAGATCGGCCTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGcc                                                                                                                                             <  7:240588/148‑1 (MQ=255)
       gCGGAGAATGGCGAGATCGCCCTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGcc                                                                                                                                             <  8:235147/148‑1 (MQ=255)
       gCGGAGAATGGCGAGATCGCCCTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCg                                                                                                                                            >  2:325324/1‑149 (MQ=255)
          gagaATGGCGAGATCGCCCTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCACGGCCTGCTGGCAGGCCgcgc                                                                                                                                         <  7:140052/149‑4 (MQ=255)
               tGGCGAGATCGCCCTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTg                                                                                                                                    >  5:693/1‑149 (MQ=255)
                 gCGAGATCGCCCTTGCACGCTGGCAGGAAGAGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGc                                                                                                                                  <  7:267292/149‑1 (MQ=255)
                      aTCGCCCTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGAc                                                                                                                             <  7:188727/149‑1 (MQ=255)
                           ccTTGCACGCCGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCa                                                                                                                        <  6:50510/149‑1 (MQ=255)
                            cTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCaa                                                                                                                       <  2:236952/149‑1 (MQ=255)
                             ttGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAAt                                                                                                                      >  2:64467/1‑149 (MQ=255)
                             ttGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAAt                                                                                                                      >  5:372339/1‑149 (MQ=255)
                                cACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGcc                                                                                                                   <  8:233799/149‑1 (MQ=255)
                                 aCGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGcc                                                                                                                   >  4:294069/1‑148 (MQ=255)
                                 aCGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGcc                                                                                                                   >  4:64655/1‑148 (MQ=255)
                                 aCGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGcc                                                                                                                   >  8:28276/1‑148 (MQ=255)
                                  cGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCa                                                                                                                 <  4:10769/149‑1 (MQ=255)
                                     tGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGc                                                                                                              >  7:136994/1‑149 (MQ=255)
                                        cAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGcc                                                                                                             >  8:88450/1‑147 (MQ=255)
                                              gcgcgcTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCg                                                                                                     <  8:183812/149‑1 (MQ=255)
                                                 cgcTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTAc                                                                                                  <  6:372339/149‑1 (MQ=255)
                                                   cTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTc                                                                                                >  2:288441/1‑149 (MQ=255)
                                                         ccTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTAtt                                                                                                                                        <  6:183548/103‑1 (MQ=255)
                                                         ccTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTAtt                                                                                                                                        >  5:183548/1‑103 (MQ=255)
                                                            ggTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTgg                                                                                       >  3:313735/1‑149 (MQ=255)
                                                            ggTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTgg                                                                                       >  3:318976/1‑149 (MQ=255)
                                                             gTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGc                                                                                      <  4:313735/149‑1 (MQ=255)
                                                             gTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGc                                                                                      <  4:318976/149‑1 (MQ=255)
                                                                   aCCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCTGGCAGGCCg                                                                                                                                            >  1:63519/1‑89 (MQ=255)
                                                                   aCCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCTGGCAGGCCg                                                                                                                                            <  2:63519/89‑1 (MQ=255)
                                                                   aCCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCg                                                                                 <  3:64655/148‑1 (MQ=255)
                                                                    ccGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCt                                                                                         <  8:85835/139‑1 (MQ=255)
                                                                    ccGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCt                                                                                         >  7:85835/1‑139 (MQ=255)
                                                                       aTTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATg                                                                            >  3:343859/1‑149 (MQ=255)
                                                                           gttcaTGCCCGTCCATGACCGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGAc                                                                         >  8:28057/4‑148 (MQ=255)
                                                                                 gCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGcc                                                                  >  2:104439/1‑149 (MQ=255)
                                                                                  ccGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCt                                                                 <  1:64467/149‑1 (MQ=255)
                                                                                        aTGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCaa                                                          <  8:136994/149‑1 (MQ=255)
                                                                                         tGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAg                                                         >  6:290837/1‑149 (MQ=255)
                                                                                             cGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAGCCGa                                                     <  7:321811/149‑1 (MQ=255)
                                                                                                gCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAGCCGATTg                                                  >  2:224617/1‑149 (MQ=255)
                                                                                                gCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAGCCGATTg                                                  >  8:38738/1‑149 (MQ=255)
                                                                                                  aTGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAGCCGATTGGt                                                <  7:28276/149‑1 (MQ=255)
                                                                                                        tCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAGCCGATTGGTCTgcgc                                          >  7:314429/1‑149 (MQ=255)
                                                                                                          aCCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAGCCGAt                                                    >  2:247506/1‑137 (MQ=255)
                                                                                                          aCCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAGCCGAt                                                    <  1:247506/137‑1 (MQ=255)
                                                                                                          aCCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAGCCGATTg                                                  <  2:328058/139‑1 (MQ=255)
                                                                                                          aCCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAGCCGATTg                                                  >  1:328058/1‑139 (MQ=255)
                                                                                                          aCCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAGCCGATTGGTCTgcgc                                          <  7:88450/147‑1 (MQ=255)
                                                                                                          aCCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAGCCGATTGGTCTGCGCAc                                        >  3:157827/1‑149 (MQ=255)
                                                                                                                 gcATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAGCCGATTGGTCTGCGCACGCTCAAg                                 <  4:157827/149‑1 (MQ=255)
                                                                                                                  cATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAGCCGATTGGTCTGCGCACGCTCAAGa                                <  1:288441/149‑1 (MQ=255)
                                                                                                                  cATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAGCCGATTGGTCTGCGCACGCTCAAGa                                <  3:294069/149‑1 (MQ=255)
                                                                                                                     tCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAGCCGATTGGTCTGCGCACGCTCAAGAccc                             >  7:7840/1‑149 (MQ=255)
                                                                                                                       gCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGc                                                                                                      >  7:244357/1‑75 (MQ=255)
                                                                                                                       gCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCAc                           >  5:372495/1‑149 (MQ=255)
                                                                                                                        cAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAGCCGATTGGTCTGCGCACGCTCAAGacacac                           >  8:142435/1‑148 (MQ=255)
                                                                                                                        cAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAGCCGATTGGGCTGCGCACGCTCAAGACCCAcc                          >  7:155970/1‑149 (MQ=255)
                                                                                                                          gCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCACCTg                        >  3:12738/1‑149 (MQ=255)
                                                                                                                                aGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCACCTGCCCCAg                  <  8:314429/149‑1 (MQ=255)
                                                                                                                                 gggCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCACCTggggcagg                 <  8:155970/149‑8 (MQ=255)
                                                                                                                                  ggCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCACCTGCCCCAGGt                <  4:343859/149‑1 (MQ=255)
                                                                                                                                  ggCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCACCTGCCCCAGGt                <  4:12738/149‑1 (MQ=255)
                                                                                                                                  ggCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCACCTGCCCCAGGt                >  6:1687/1‑149 (MQ=255)
                                                                                                                                    cGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCACCTGCCCCAGGTgc              <  7:28057/149‑1 (MQ=255)
                                                                                                                                     gaatacgGCCTGCTGGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCACCTGCCCCAGGTgcg             >  7:58754/6‑149 (MQ=255)
                                                                                                                                     gAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCACCTGCCCCAGGTgcg             >  3:123398/1‑149 (MQ=255)
                                                                                                                                       gcgcGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCACCTGCCCCAGGTGCGcc           <  6:372495/149‑1 (MQ=255)
                                                                                                                                           ggCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTGTTTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCACCTGCCCCAGGTGCGCCCTc        <  1:93423/149‑1 (MQ=255)
                                                                                                                                              cTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCACCTGCCCCAGGTGCGCCCTcagca    <  5:1687/149‑1 (MQ=255)
                                                                                                                                               tGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCACCTGCCCCAGGTGCGCCCTcagcag   >  3:167029/1‑149 (MQ=255)
                                                                                                                                               tGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCACCTGCCCCAGGTGCGCCCTcagcag   >  2:173698/1‑149 (MQ=255)
                                                                                                                                                 ccAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCACCTGCCCCAGGTGCGCCCTCagcagc  <  8:7840/148‑1 (MQ=255)
                                                                                                                                                   agcagGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCACCTGCCCCAg                  >  4:14379/1‑130 (MQ=255)
                                                                                                                                                   agcagGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCACCTGCCCCAg                  <  3:14379/130‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GACACTGGCGGAGAATGGCGAGATCGCCCTTGCACGCTGGCAGGAAGCGCGCTTCGACCTGGTGATCACCGATTGCAGCATGCCGGTCATGGACGGGCATGAACTCACCCGGCGCATTCGCAGCCTGGAGGGCGAGCGCGGCCTGCCAGCAGGCCGTATTCTTGGCGTGACCGCCAATGCCCAGGCCGAGGAGCGTACTCGCTGCCTGGCCAGCGGCATGGACGAATGCCTG‑TTCAAGCCGATTGGTCTGCGCACGCTCAAGACCCACCTGCCCCAGGTGCGCCCTCAGCAGC  >  NC_002947/3863693‑3863985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑