Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,891,050 A→G 5.0% S12G (AGC→GGC)  PP_3435 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,891,0500AG 5.0% 242.5 / 2.1 79S12G (AGC→GGC) PP_3435hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (39/36);  new base G (2/2);  total (41/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

ATTCGTAAATCGATTCTACATTGCGTAAAATGGTTTGTCGCGTTTTAGTTGCTCGTACGCGGCGACAAACGGCTATCCTCTCGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTGAAATGCAGGCCAGCAGCCACGCCACCACCCGCCAGGCGTTGGAGCACGTCGAACACATCCTCGA  >  NC_002947/3890907‑3891198
                                                                                                                                                |                                                                                                                                                     
aTTCGTAAATCGATTCTACATTGCGTAAAATGGTTTGTCGCGTTTTAGTTGCTCGTACGCGGCGACAAACGGCTATCCTCTCGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTgg                                                                                                                                                  >  6:58891/1‑149 (MQ=255)
 ttCGTAAATCGATTCTACATTGCGTAAAATGGTTTGTCGCGTTTTAGTTGCTCGTACGCGGCGACAAACGGCTATCCTCTCGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTgg                                                                                                                                                  <  5:80605/148‑1 (MQ=255)
    gTAAATCGATTCTACATTGCGTAAAATGGTTTGTCGCGTTTTAGTTGCTCGTACGCGGCGACAAACGGCTATCCTCTCGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCa                                                                                                                                              >  3:329335/1‑149 (MQ=255)
       aaTCGATTCTACATTGCGTAAAATGGTTTGTCGCGTTTTAGTTGCTCGTACGCGGCGACAAACGGCTATCCTCTCGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑Gc                                                                                                                                          >  5:234807/1‑149 (MQ=255)
         tCGATACTACATTGCGTAAAATGGTTTGTCGCGTTTTAGTTGCTCGTACGCGGCGACAAACGGCTATCCTCTCGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑Gctc                                                                                                                                        <  1:359206/149‑1 (MQ=255)
             ttCTACATTGCGTAAAATGGTTTGTCGCGTTTTAGTTGCTCGTACGCGGCGACAAACGGCTATCCTCTCGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGc                                                                                                                                    >  3:225653/1‑149 (MQ=255)
                tACATTGCGTAAAATGGTTTGTCGCGTTTTAGTTGCTCGTACGCGGCGACAAACGGCTATCCTCTCGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAA‑GCTCTTGCCCt                                                                                                                                 >  5:170431/1‑149 (MQ=255)
                 aCATTGCGTAAAATGGTTTGTCGCGTTTTAGTTGCTCGTACGCGGCGACAAACGGCTATCCTCTCGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTg                                                                                                                                >  1:172892/1‑149 (MQ=255)
                 aCATTGCGTAAAATGGTTTGTCGCGTTTTAGTTGCTCGTACGCGGCGACAAACGGCTATCCTCTCGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTg                                                                                                                                >  1:340595/1‑149 (MQ=255)
                  cATTGCGTAAATTGGTTTGTCGCGTTTTAGTTGCTCGTACGCGGCGACAAACGGCTATCCTCTCGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCt                                                                                                                                 <  3:70680/147‑1 (MQ=255)
                     tGCGTAAAATGGTTTGTCGCGTTTTAGTTGCTCGTACGCGGCGACAAACGGCTATCCTCTCGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGgtc                                                                                                                            >  4:124898/1‑149 (MQ=255)
                          cacgggacaggttgtcgaggatgtgttcgacgtgctccaacgcctggcgggtggtggcgtggctgctggcctgcatttcacgttcgacctgccagttcatcacggccagGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGtcgtcg                                                                                                                        <  6:123122‑M1/40‑1 (MQ=255)
                            aaTGGTTTGTCGCGTTTTAGTTGCTCGTACGCGGCGACAAACGGCTATCCTCTCGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCg                                                                                                                     <  8:225070/149‑1 (MQ=255)
                             aTGGTTTGTCGCGTTTTAGTTGCTCGTACGCGGCGACAAACGGCTATCCTCTCGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGt                                                                                                                    <  2:23420/149‑1 (MQ=255)
                                gTTTGTCGCGTTTTAGTTGCTCGTACGCGGCGACAAACGGCTATCCTCTCGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGtgct                                                                                                                 >  6:121436/1‑149 (MQ=255)
                                gTTTGTCGCGTTTTAGTTGCTCGTACGCGGCGACAAACGGCTATCCTCTCGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGtgc                                                                                                                  <  4:329335/148‑1 (MQ=255)
                                 tttGTCGCGTTTTAGTTGCTCGTACGCGGCGACAAACGGCTATCCTCTCGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGtgctg                                                                                                                >  4:73085/1‑149 (MQ=255)
                                     tCGCGTTTTAGTTGCTCGTACGCGGCGACAAACGGCTATCCTCTCGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCg                                                                                                             <  3:124898/148‑1 (MQ=255)
                                          gaggatgtgttcgacgtgctccaacgcctggcgggtggtggcgtggctgctggcctgcatttcacgttcgacctgccagttcatcacggccagGCCGCACATGACCGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCg                                                                                                             >  4:1791‑M1/94‑144 (MQ=255)
                                          gaggatgtgttcgacgtgctccaacgcctggcgggtggtggcgtggctgctggcctgcatttcacgttcgacctgccagttcatcacggccagGCCGCACATGACCGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCg                                                                                                             <  3:1791‑M1/51‑1 (MQ=255)
                                               gTTGCTCGTACGCGGCGACAAACGGCTATCCTCTCGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCgg                                                                                                  >  3:260492/1‑149 (MQ=255)
                                               gTTGCTCGTACGCGGCGACAAACGGCTATCCTCTCGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCg                                                                                                   <  6:234807/148‑1 (MQ=255)
                                                 tgttcgacgtgctccaacgcctggcgggtggtggcgtggctgctggcctgcatttcacattcgacctgccagttcatcacggccagGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGc                                                                                                 >  6:133253‑M1/87‑149 (MQ=255)
                                                   ttcgacgtgctccaacgcctggcgggtggtggcgtggctgctggcctgcatttcacgttcgacctgccagttcatcacggccagGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGcc                                                                                                >  5:123122‑M1/85‑148 (MQ=255)
                                                   cTCGTACGCGGCGACAAACGGCTATCCTCTCGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCGGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTg                                                                                              >  2:89838/1‑149 (MQ=255)
                                                   cTCGTACGCGGCGACAAACGGCTATCCTCTCGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCt                                                                                               <  2:172892/148‑1 (MQ=255)
                                                    tCGTACGCGGCGACAAACGGCTATCCTCTCGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTgg                                                                                             >  4:14938/1‑149 (MQ=255)
                                                      gTACGCGGCGACAAACGGCTATCCTCTCGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTgg                                                                                             <  5:235413/147‑1 (MQ=255)
                                                       tACGCGGCGACAAACGGCTATCCTCTCGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCACGGCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGcc                                                                                           <  1:261280/149‑1 (MQ=255)
                                                         cgcgGCGACAAACGGCTATCCTCTCGTTTTAACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCGC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGtg                                                                                        <  7:253148/149‑1 (MQ=255)
                                                          gcggcgACAAACGGCTATCCTCTCGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCGGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGtga                                                                                       <  1:89838/149‑1 (MQ=255)
                                                          gcggcgACAAACGGCTATCCTCTCGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGtga                                                                                       <  5:121436/149‑1 (MQ=255)
                                                           cggcgACAAACGGCTATCCTCTCGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGtgat                                                                                      <  8:186483/149‑1 (MQ=255)
                                                           cggcgACAAACGGCTATCCTCTCGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGtgat                                                                                      <  4:260492/149‑1 (MQ=255)
                                                           cggcgACAAACGGCTATCCTCTCGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGtgat                                                                                      >  1:61724/1‑149 (MQ=255)
                                                               gACAAACGGCTATCCTCTCGTTTTTACTCCATGATAGTCAGATCAGCATCCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTCCACTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATCaa                                                                                   >  2:300638/1‑148 (MQ=255)
                                                                  aaaCGGCTATCCTCTCGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTgg                                                                               >  2:83990/1‑149 (MQ=255)
                                                                              tctcGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGTGCAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACg                                                                    >  1:78617/1‑149 (MQ=255)
                                                                               ctcGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGt                                                                   >  5:25114/1‑148 (MQ=255)
                                                                                tcGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGtga                                                                                       >  7:300039/1‑127 (MQ=255)
                                                                                tcGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGtga                                                                                       <  8:300039/127‑1 (MQ=255)
                                                                                tcGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGt                                                                   <  8:157023/147‑1 (MQ=255)
                                                                                tcGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGt                                                                   >  7:157023/1‑147 (MQ=255)
                                                                                  gTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTg                                                                  <  5:58891/146‑1 (MQ=255)
                                                                                  gTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTGaaa                                                               >  6:247071/1‑149 (MQ=255)
                                                                                  gTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTGa                                                                 <  1:46893/147‑1 (MQ=255)
                                                                                      ttACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTGAAATGc                                                            <  4:225653/148‑1 (MQ=255)
                                                                                        aCTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTGAAATGCAg                                                          <  6:25114/148‑1 (MQ=255)
                                                                                          tCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAg                                                                            >  4:255830/1‑128 (MQ=255)
                                                                                            cATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGc                                                                                            >  4:234463/1‑110 (MQ=255)
                                                                                            cATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGc                                                                                            <  3:234463/110‑1 (MQ=255)
                                                                                            cATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTGAAATGCAGGCcag                                                     >  3:76567/1‑149 (MQ=255)
                                                                                             aTGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTGAAAt                                                              >  5:131083/1‑139 (MQ=255)
                                                                                             aTGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTGAAAt                                                              <  6:131083/139‑1 (MQ=255)
                                                                                               gATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTGAAATGc                                                            <  1:219711/139‑1 (MQ=255)
                                                                                               gATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTGAAATGc                                                            >  2:219711/1‑139 (MQ=255)
                                                                                                aTAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTGAAATGCAGGCcagcag                                                  <  1:212280/148‑1 (MQ=255)
                                                                                                      aGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTGAAATGCAGGCCAGCAgccacgcc                                           >  5:280924/1‑149 (MQ=255)
                                                                                                       gATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTGAAATGCAGGCCAGCAgccacgcca                                          <  3:73085/149‑1 (MQ=255)
                                                                                                            gCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTGAAATGCAGGCCAGCAGCCACGCCaccacc                                     <  1:83990/149‑1 (MQ=255)
                                                                                                               tGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTGAAATGCAGGCCAGCAGCCACGCCACCACCCGc                                  <  2:78617/149‑1 (MQ=255)
                                                                                                                 ccccTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTGAAATGCCGGCCAGCAGCCACGCCACCACCCGCCa                                <  6:170431/149‑1 (MQ=255)
                                                                                                                  cccTTACCGT‑CAAACGCCGCGCCGCACTGAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTGAAATGCAGGCCAGCAGCCACGCCACCACCCGCCAg                               >  1:205470/1‑149 (MQ=255)
                                                                                                                  cccTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTGAAATGCAGGCCAGCAGCCACGCCACCACCCGCCAg                               >  1:174549/1‑149 (MQ=255)
                                                                                                                  cccTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTGAAATGCAGGCCAGCAGCCACGCCACCACCCGCCAg                               >  4:207940/1‑149 (MQ=255)
                                                                                                                         cGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTGAAATGCAGGCCAGCAGCCACGCCACCACCCGCCAGGCGTTg                         >  3:177166/1‑148 (MQ=255)
                                                                                                                          gT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTGAAATGCAGGCCAGCAgccac                                              >  7:140541/1‑126 (MQ=255)
                                                                                                                          gT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTGAAATGCAGGCCAGCAgccac                                              <  8:140541/126‑1 (MQ=255)
                                                                                                                           t‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTGAAATGCAGGCCAGCAGCCACGCCACCACCCGCCa                                >  5:88708/1‑139 (MQ=255)
                                                                                                                           t‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTGAAATGCAGGCCAGCAGCCACGCCACCACCCGCCa                                <  6:88708/139‑1 (MQ=255)
                                                                                                                             cAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTGAAATGCAGGCCAGCAGCCACGCCACCACCCGCCAGGCGTTGGAg                      >  1:240877/1‑148 (MQ=255)
                                                                                                                              aaaCGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTGAAATGCAGGCCAGCAGCCACGCCACCACCCGCCAGGCGTTGGAGc                     <  4:177166/148‑1 (MQ=255)
                                                                                                                              aaaCGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTGAAATGCAGGCCAGCAGCCACGCCACCACCCGCCAGGCGTTGGAGCa                    >  5:307376/1‑149 (MQ=255)
                                                                                                                               aaCGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTGAAATGCAGGCCAGCAGCCACGCCACCACCCGCCAGGCGTTGGAGCAc                   >  5:232467/1‑149 (MQ=255)
                                                                                                                               aaCGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTGAAATGCAGGCCAGCAGCCACGCCACCACCCGCCAGGCGTTGGAGCAc                   <  2:340595/149‑1 (MQ=255)
                                                                                                                                aCGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTGAAATGCAGGCCAGCAGCCACGCCACCACCCGCCAGGCGTTGGAGCACg                  >  8:297972/1‑149 (MQ=255)
                                                                                                                                 cgccgcgccgCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTGAAATGCAGGCCAGCAGCCACGCCACCACCCGCCAGGCGTTGGAGCACGt                 <  6:280924/149‑1 (MQ=255)
                                                                                                                                              tCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTGAAATGCAGGCCAGCAGCCACGCCaccacc                                     >  5:200806/1‑116 (MQ=255)
                                                                                                                                              tCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTGAAATGCAGGCCAGCAGCCACGCCaccacc                                     <  6:200806/116‑1 (MQ=255)
                                                                                                                                                aGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTGAAATGCAGGCCAGCAGCCACGCCACCACCCGCCAGGCGTTGGAGCACGTCGAACACATCCTCg   <  2:205470/148‑1 (MQ=255)
                                                                                                                                                aGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTGAAATGCAGGCCAGCAGCCACGCCACCACCCGCCAGGCGTTGGAGCACGTCGAACACATCCTCGa  >  7:310047/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
ATTCGTAAATCGATTCTACATTGCGTAAAATGGTTTGTCGCGTTTTAGTTGCTCGTACGCGGCGACAAACGGCTATCCTCTCGTTTTTACTCCATGATAGTCAGATCAGCATGCCCCTTACCGT‑CAAACGCCGCGCCGCACTCAGCTGGCGCAC‑GCTCTTGCCCTGGGTCGTCGGCGTGCTGCCGGTCATGTGCGGCCTGGCCGTGATGAACTGGCAGGTCGAACGTGAAATGCAGGCCAGCAGCCACGCCACCACCCGCCAGGCGTTGGAGCACGTCGAACACATCCTCGA  >  NC_002947/3890907‑3891198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑